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Protein

Origin recognition complex subunit 1

Gene

ORC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi479 – 486ATPSequence analysis8
Nucleotide bindingi726 – 733ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: SGD
  • chromatin binding Source: SGD
  • DNA replication origin binding Source: SGD

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • DNA replication initiation Source: SGD
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32660-MONOMER.
ReactomeiR-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-68616. Assembly of the ORC complex at the origin of replication.
R-SCE-68689. CDC6 association with the ORC:origin complex.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-68962. Activation of the pre-replicative complex.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69298. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 1
Alternative name(s):
Origin recognition complex 120 kDa subunit
Gene namesi
Name:ORC1
Ordered Locus Names:YML065W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML065W.
SGDiS000004530. ORC1.

Subcellular locationi

GO - Cellular componenti

  • DNA replication preinitiation complex Source: SGD
  • nuclear origin of replication recognition complex Source: SGD
  • nuclear pre-replicative complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001270741 – 914Origin recognition complex subunit 1Add BLAST914

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei237PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP54784.
PRIDEiP54784.

PTM databases

iPTMnetiP54784.

Interactioni

Subunit structurei

Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. Interacts with MCM10 and TAH11.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ORC4P547914EBI-12568,EBI-12580

Protein-protein interaction databases

BioGridi35101. 331 interactors.
DIPiDIP-2284N.
IntActiP54784. 34 interactors.
MINTiMINT-616045.

Structurei

Secondary structure

1914
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 9Combined sources4
Beta strandi11 – 16Combined sources6
Beta strandi18 – 20Combined sources3
Beta strandi38 – 43Combined sources6
Turni44 – 46Combined sources3
Beta strandi55 – 60Combined sources6
Turni61 – 64Combined sources4
Beta strandi65 – 76Combined sources12
Turni78 – 80Combined sources3
Beta strandi83 – 91Combined sources9
Helixi93 – 95Combined sources3
Helixi98 – 105Combined sources8
Helixi107 – 111Combined sources5
Helixi116 – 126Combined sources11
Beta strandi131 – 141Combined sources11
Helixi143 – 145Combined sources3
Beta strandi146 – 153Combined sources8
Helixi155 – 161Combined sources7
Helixi162 – 164Combined sources3
Turni167 – 169Combined sources3
Beta strandi170 – 176Combined sources7
Turni179 – 181Combined sources3
Beta strandi182 – 186Combined sources5
Helixi189 – 196Combined sources8
Helixi201 – 210Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M4ZX-ray2.20A/B1-235[»]
1ZBXX-ray2.50A1-219[»]
1ZHIX-ray2.70A1-219[»]
ProteinModelPortaliP54784.
SMRiP54784.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54784.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 188BAHPROSITE-ProRule annotationAdd BLAST141

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi281 – 292Poly-GluAdd BLAST12
Compositional biasi759 – 768Poly-Asp10

Domaini

The N-terminus is dedicated to mating-type repression.

Sequence similaritiesi

Belongs to the ORC1 family.Curated
Contains 1 BAH domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000063498.
HOGENOMiHOG000115288.
InParanoidiP54784.
KOiK02603.
OMAiLKVCKRA.
OrthoDBiEOG092C0XFO.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51038. BAH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTLKDLQG WEIITTDEQG NIIDGGQKRL RRRGAKTEHY LKRSSDGIKL
60 70 80 90 100
GRGDSVVMHN EAAGTYSVYM IQELRLNTLN NVVELWALTY LRWFEVNPLA
110 120 130 140 150
HYRQFNPDAN ILNRPLNYYN KLFSETANKN ELYLTAELAE LQLFNFIRVA
160 170 180 190 200
NVMDGSKWEV LKGNVDPERD FTVRYICEPT GEKFVDINIE DVKAYIKKVE
210 220 230 240 250
PREAQEYLKD LTLPSKKKEI KRGPQKKDKA TQTAQISDAE TRATDITDNE
260 270 280 290 300
DGNEDESSDY ESPSDIDVSE DMDSGEISAD ELEEEEDEEE DEDEEEKEAR
310 320 330 340 350
HTNSPRKRGR KIKLGKDDID ASVQPPPKKR GRKPKDPSKP RQMLLISSCR
360 370 380 390 400
ANNTPVIRKF TKKNVARAKK KYTPFSKRFK SIAAIPDLTS LPEFYGNSSE
410 420 430 440 450
LMASRFENKL KTTQKHQIVE TIFSKVKKQL NSSYVKEEIL KSANFQDYLP
460 470 480 490 500
ARENEFASIY LSAYSAIESD SATTIYVAGT PGVGKTLTVR EVVKELLSSS
510 520 530 540 550
AQREIPDFLY VEINGLKMVK PTDCYETLWN KVSGERLTWA ASMESLEFYF
560 570 580 590 600
KRVPKNKKKT IVVLLDELDA MVTKSQDIMY NFFNWTTYEN AKLIVIAVAN
610 620 630 640 650
TMDLPERQLG NKITSRIGFT RIMFTGYTHE ELKNIIDLRL KGLNDSFFYV
660 670 680 690 700
DTKTGNAILI DAAGNDTTVK QTLPEDVRKV RLRMSADAIE IASRKVASVS
710 720 730 740 750
GDARRALKVC KRAAEIAEKH YMAKHGYGYD GKTVIEDENE EQIYDDEDKD
760 770 780 790 800
LIESNKAKDD NDDDDDNDGV QTVHITHVMK ALNETLNSHV ITFMTRLSFT
810 820 830 840 850
AKLFIYALLN LMKKNGSQEQ ELGDIVDEIK LLIEVNGSNK FVMEIAKTLF
860 870 880 890 900
QQGSDNISEQ LRIISWDFVL NQLLDAGILF KQTMKNDRIC CVKLNISVEE
910
AKRAMNEDET LRNL
Length:914
Mass (Da):104,400
Last modified:October 1, 1996 - v1
Checksum:iBDAA9D3E40B4B76A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34860 Genomic DNA. Translation: AAB38248.1.
Z38114 Genomic DNA. Translation: CAA86256.1.
BK006946 Genomic DNA. Translation: DAA09832.1.
PIRiS48333.
RefSeqiNP_013646.1. NM_001182424.1.

Genome annotation databases

EnsemblFungiiYML065W; YML065W; YML065W.
GeneIDi854937.
KEGGisce:YML065W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34860 Genomic DNA. Translation: AAB38248.1.
Z38114 Genomic DNA. Translation: CAA86256.1.
BK006946 Genomic DNA. Translation: DAA09832.1.
PIRiS48333.
RefSeqiNP_013646.1. NM_001182424.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M4ZX-ray2.20A/B1-235[»]
1ZBXX-ray2.50A1-219[»]
1ZHIX-ray2.70A1-219[»]
ProteinModelPortaliP54784.
SMRiP54784.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35101. 331 interactors.
DIPiDIP-2284N.
IntActiP54784. 34 interactors.
MINTiMINT-616045.

PTM databases

iPTMnetiP54784.

Proteomic databases

MaxQBiP54784.
PRIDEiP54784.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML065W; YML065W; YML065W.
GeneIDi854937.
KEGGisce:YML065W.

Organism-specific databases

EuPathDBiFungiDB:YML065W.
SGDiS000004530. ORC1.

Phylogenomic databases

GeneTreeiENSGT00530000063498.
HOGENOMiHOG000115288.
InParanoidiP54784.
KOiK02603.
OMAiLKVCKRA.
OrthoDBiEOG092C0XFO.

Enzyme and pathway databases

BioCyciYEAST:G3O-32660-MONOMER.
ReactomeiR-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-68616. Assembly of the ORC complex at the origin of replication.
R-SCE-68689. CDC6 association with the ORC:origin complex.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-68962. Activation of the pre-replicative complex.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69298. Association of licensing factors with the pre-replicative complex.

Miscellaneous databases

EvolutionaryTraceiP54784.
PROiP54784.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR001025. BAH_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF01426. BAH. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00439. BAH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51038. BAH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiORC1_YEAST
AccessioniPrimary (citable) accession number: P54784
Secondary accession number(s): D6VZA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3930 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.