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P54783

- ALO_YEAST

UniProt

P54783 - ALO_YEAST

Protein

D-arabinono-1,4-lactone oxidase

Gene

ALO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Can oxidize L-gulono-1,4-lactone as well as D-arabinono-1,4-lactone and L-galactono-1,4-lactone.

    Catalytic activityi

    D-arabinono-1,4-lactone + O2 = dehydro-D-arabinono-1,4-lactone + H2O2.

    Cofactori

    FAD.

    Pathwayi

    GO - Molecular functioni

    1. D-arabinono-1,4-lactone oxidase activity Source: SGD
    2. flavin adenine dinucleotide binding Source: InterPro
    3. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro

    GO - Biological processi

    1. cellular response to oxidative stress Source: SGD
    2. dehydro-D-arabinono-1,4-lactone biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMetaCyc:YML086C-MONOMER.
    YEAST:YML086C-MONOMER.
    UniPathwayiUPA00771; UER00766.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-arabinono-1,4-lactone oxidase (EC:1.1.3.37)
    Short name:
    ALO
    Alternative name(s):
    L-galactono-gamma-lactone oxidase
    Gene namesi
    Name:ALO1
    Ordered Locus Names:YML086C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML086c.
    SGDiS000004551. ALO1.

    Subcellular locationi

    Mitochondrion membrane
    Note: Membrane-embedded.

    GO - Cellular componenti

    1. integral component of mitochondrial outer membrane Source: SGD
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 526526D-arabinono-1,4-lactone oxidasePRO_0000128171Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei56 – 561Pros-8alpha-FAD histidineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    MaxQBiP54783.
    PaxDbiP54783.
    PeptideAtlasiP54783.

    Expressioni

    Gene expression databases

    GenevestigatoriP54783.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-2519,EBI-2519
    ACB1P317871EBI-2519,EBI-2060
    ADE17P380091EBI-2519,EBI-14223
    ARF1P110761EBI-2519,EBI-2816
    ARO8P530901EBI-2519,EBI-2042933
    CWP2P434971EBI-2519,EBI-2044078
    CYS4P325821EBI-2519,EBI-4167
    DAP1Q120911EBI-2519,EBI-5554
    DBP5P204491EBI-2519,EBI-5617
    DNM1P548611EBI-2519,EBI-6002
    EHT1P382951EBI-2519,EBI-20890
    FAA3P390021EBI-2519,EBI-10089
    FUN14P184111EBI-2519,EBI-20609
    GLN1P322881EBI-2519,EBI-7665
    GTT1P405821EBI-2519,EBI-7941
    HSP12P229431EBI-2519,EBI-8548
    HUG1Q6Q5K61EBI-2519,EBI-392766
    HYR1P405811EBI-2519,EBI-7869
    IML2P470311EBI-2519,EBI-25966
    INO1P119861EBI-2519,EBI-9257
    IPP1P008171EBI-2519,EBI-9338
    KES1P358441EBI-2519,EBI-9648
    LSP1Q122301EBI-2519,EBI-34978
    MCR1P360601EBI-2519,EBI-10565
    MSP1P287371EBI-2519,EBI-11435
    OYE2Q035581EBI-2519,EBI-12729
    PCK1P109631EBI-2519,EBI-13770
    PGM2P370121EBI-2519,EBI-13296
    PMP3P872841EBI-2519,EBI-13555
    QCR9P222891EBI-2519,EBI-19947
    RNR1P215241EBI-2519,EBI-15234
    RNR2P099381EBI-2519,EBI-15240
    SAH1P399541EBI-2519,EBI-16451
    SAM35P146931EBI-2519,EBI-24602
    SEC4P075601EBI-2519,EBI-16858
    SIS1P252941EBI-2519,EBI-17244
    SNC2P333281EBI-2519,EBI-17512
    SNF7P399291EBI-2519,EBI-17554
    SSL2Q005781EBI-2519,EBI-14683
    TOM20P351801EBI-2519,EBI-12522
    TOM70P072131EBI-2519,EBI-12551
    TSC10P383421EBI-2519,EBI-21124
    UIP4Q089261EBI-2519,EBI-2044051
    URA7P282741EBI-2519,EBI-20128
    VAS1P07806-11EBI-2519,EBI-1009942From a different organism.
    YBL039W-BP0C2681EBI-2519,EBI-2044092
    YDR381C-AQ3E6R51EBI-2519,EBI-2044020
    YGR012WP532061EBI-2519,EBI-2044073
    YHR003CP387561EBI-2519,EBI-24431
    YHR146WP388451EBI-2519,EBI-24770
    YPT1P011231EBI-2519,EBI-29496
    YPT31P385551EBI-2519,EBI-29379
    YPT32P519961EBI-2519,EBI-29384

    Protein-protein interaction databases

    BioGridi35055. 53 interactions.
    IntActiP54783. 39 interactions.
    MINTiMINT-4497062.

    Structurei

    3D structure databases

    ProteinModelPortaliP54783.
    SMRiP54783. Positions 14-204.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 193175FAD-binding PCMH-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0277.
    GeneTreeiENSGT00510000049722.
    HOGENOMiHOG000204635.
    KOiK00107.
    OMAiMDCLFSQ.
    OrthoDBiEOG7JX3CZ.

    Family and domain databases

    Gene3Di3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProiIPR007173. ALO.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR010031. FAD_lactone_oxidase.
    IPR023595. LGO_GLO.
    IPR006094. Oxid_FAD_bind_N.
    IPR006093. Oxy_OxRdtase_FAD_BS.
    [Graphical view]
    PfamiPF04030. ALO. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000136. LGO_GLO. 1 hit.
    SUPFAMiSSF56176. SSF56176. 1 hit.
    TIGRFAMsiTIGR01678. FAD_lactone_ox. 1 hit.
    PROSITEiPS51387. FAD_PCMH. 1 hit.
    PS00862. OX2_COVAL_FAD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54783-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTIPFRKNY VFKNWAGIYS AKPERYFQPS SIDEVVELVK SARLAEKSLV    50
    TVGSGHSPSN MCVTDEWLVN LDRLDKVQKF VEYPELHYAD VTVDAGMRLY 100
    QLNEFLGAKG YSIQNLGSIS EQSVAGIIST GSHGSSPYHG LISSQYVNLT 150
    IVNGKGELKF LDAENDPEVF KAALLSVGKI GIIVSATIRV VPGFNIKSTQ 200
    EVITFENLLK QWDTLWTSSE FIRVWWYPYT RKCVLWRGNK TTDAQNGPAK 250
    SWWGTKLGRF FYETLLWIST KIYAPLTPFV EKFVFNRQYG KLEKSSTGDV 300
    NVTDSISGFN MDCLFSQFVD EWGCPMDNGL EVLRSLDHSI AQAAINKEFY 350
    VHVPMEVRCS NTTLPSEPLD TSKRTNTSPG PVYGNVCRPF LDNTPSHCRF 400
    APLENVTNSQ LTLYINATIY RPFGCNTPIH KWFTLFENTM MVAGGKPHWA 450
    KNFLGSTTLA AGPVKKDTDY DDFEMRGMAL KVEEWYGEDL KKFRKIRKEQ 500
    DPDNVFLANK QWAIINGIID PSELSD 526
    Length:526
    Mass (Da):59,494
    Last modified:October 1, 1996 - v1
    Checksum:i942177A74A738EC8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti417 – 4171A → P in BAA23804. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U40390 Genomic DNA. Translation: AAC98538.1.
    AB009401 Genomic DNA. Translation: BAA23804.1.
    Z46660 Genomic DNA. Translation: CAA86652.1.
    AY693120 Genomic DNA. Translation: AAT93139.1.
    BK006946 Genomic DNA. Translation: DAA09811.1.
    PIRiS49641.
    RefSeqiNP_013624.1. NM_001182445.1.

    Genome annotation databases

    EnsemblFungiiYML086C; YML086C; YML086C.
    GeneIDi854888.
    KEGGisce:YML086C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U40390 Genomic DNA. Translation: AAC98538.1 .
    AB009401 Genomic DNA. Translation: BAA23804.1 .
    Z46660 Genomic DNA. Translation: CAA86652.1 .
    AY693120 Genomic DNA. Translation: AAT93139.1 .
    BK006946 Genomic DNA. Translation: DAA09811.1 .
    PIRi S49641.
    RefSeqi NP_013624.1. NM_001182445.1.

    3D structure databases

    ProteinModelPortali P54783.
    SMRi P54783. Positions 14-204.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35055. 53 interactions.
    IntActi P54783. 39 interactions.
    MINTi MINT-4497062.

    Proteomic databases

    MaxQBi P54783.
    PaxDbi P54783.
    PeptideAtlasi P54783.

    Protocols and materials databases

    DNASUi 854888.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML086C ; YML086C ; YML086C .
    GeneIDi 854888.
    KEGGi sce:YML086C.

    Organism-specific databases

    CYGDi YML086c.
    SGDi S000004551. ALO1.

    Phylogenomic databases

    eggNOGi COG0277.
    GeneTreei ENSGT00510000049722.
    HOGENOMi HOG000204635.
    KOi K00107.
    OMAi MDCLFSQ.
    OrthoDBi EOG7JX3CZ.

    Enzyme and pathway databases

    UniPathwayi UPA00771 ; UER00766 .
    BioCyci MetaCyc:YML086C-MONOMER.
    YEAST:YML086C-MONOMER.

    Miscellaneous databases

    NextBioi 977843.
    PROi P54783.

    Gene expression databases

    Genevestigatori P54783.

    Family and domain databases

    Gene3Di 3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    InterProi IPR007173. ALO.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR010031. FAD_lactone_oxidase.
    IPR023595. LGO_GLO.
    IPR006094. Oxid_FAD_bind_N.
    IPR006093. Oxy_OxRdtase_FAD_BS.
    [Graphical view ]
    Pfami PF04030. ALO. 1 hit.
    PF01565. FAD_binding_4. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000136. LGO_GLO. 1 hit.
    SUPFAMi SSF56176. SSF56176. 1 hit.
    TIGRFAMsi TIGR01678. FAD_lactone_ox. 1 hit.
    PROSITEi PS51387. FAD_PCMH. 1 hit.
    PS00862. OX2_COVAL_FAD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "D-erythroascorbic acid is an important antioxidant molecule in Saccharomyces cerevisiae."
      Huh W.-K., Lee B.-H., Kim S.-T., Kim Y.-R., Rhie G.-E., Baek Y.-W., Hwang C.-S., Lee J.-S., Kang S.-O.
      Mol. Microbiol. 30:895-903(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 86-105 AND 349-363.
      Strain: ATCC 44774 / DBY747.
    2. "Identification of the yeast genomic sequence encoding L-galactono-gamma-lactone oxidase."
      Nishikimi M., Ohata Y., Ishikawa T.
      Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiALO_YEAST
    AccessioniPrimary (citable) accession number: P54783
    Secondary accession number(s): D6W0J7, O42618
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6190 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3