Reviewed,
UniProtKB/Swiss-Prot P54783 (ALO_YEAST)
Last modified
November 3, 2009.
Version 85.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: D-arabinono-1,4-lactone oxidase Short name=ALO EC=1.1.3.37 Alternative name(s): L-galactono-gamma-lactone oxidase | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 526 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Can oxidize L-gulono-1,4-lactone as well as D-arabinono-1,4-lactone and L-galactono-1,4-lactone. |
| Catalytic activity | D-arabinono-1,4-lactone + O2 = dehydro-D-arabinono-1,4-lactone + H2O2. |
| Cofactor | FAD. |
| Pathway | |
| Subcellular location | Mitochondrion membrane. Note: Membrane-embedded. |
| Post-translational modification | The N-terminus is blocked. |
| Miscellaneous | Present with 6190 molecules/cell in log phase SD medium. Ref.5 |
| Sequence similarities | Belongs to the oxygen-dependent FAD-linked oxidoreductase family. Contains 1 FAD-binding PCMH-type domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane Mitochondrion |
| Ligand | FAD Flavoprotein |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | dehydro-D-arabinono-1,4-lactone biosynthetic process Ref.1 Inferred from mutant phenotype. Source: SGD oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Ref.1Inferred from mutant phenotype. Source: SGD |
| Cellular component | integral to mitochondrial outer membrane Inferred from direct assay. Source: SGD |
| Molecular function | D-arabinono-1,4-lactone oxidase activity Ref.1 Inferred from direct assay. Source: SGD FAD bindingInferred from electronic annotation. Source: InterPro electron carrier activityInferred from electronic annotation. Source: InterPro identical protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 526 | 526 | D-arabinono-1,4-lactone oxidase | PRO_0000128171 | |||||
Regions | |||||||||
| Domain | 19 – 193 | 175 | FAD-binding PCMH-type | ||||||
Amino acid modifications | |||||||||
| Modified residue | 56 | 1 | Tele-8alpha-FAD histidine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 417 | 1 | A → P in BAA23804. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "D-erythroascorbic acid is an important antioxidant molecule in Saccharomyces cerevisiae." Huh W.-K., Lee B.-H., Kim S.-T., Kim Y.-R., Rhie G.-E., Baek Y.-W., Hwang C.-S., Lee J.-S., Kang S.-O. Mol. Microbiol. 30:895-903(1998) [PubMed: 10094636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 86-105 AND 349-363. Strain: ATCC 44774 / DBY747. |
| [2] | "Identification of the yeast genomic sequence encoding L-galactono-gamma-lactone oxidase." Nishikimi M., Ohata Y., Ishikawa T. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.  Barrell B.G.Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [4] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U40390 Genomic DNA. Translation: AAC98538.1. AB009401 Genomic DNA. Translation: BAA23804.1. Z46660 Genomic DNA. Translation: CAA86652.1. AY693120 Genomic DNA. Translation: AAT93139.1. | |
| PIR | S49641. |
| RefSeq | NP_013624.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P54783. 64 interactions. |
| STRING | P54783. |
Proteomic databases | |
| PeptideAtlas | P54783. |
| PRIDE | P54783. |
Genome annotation databases | |
| Ensembl | YML086C; YML086C; YML086C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 854888. |
| GenomeReviews | Gene locus YML086C in contig Z71257_GR. |
| KEGG | sce:YML086C. |
| NMPDR | fig|4932.3.peg.4661. |
Organism-specific databases | |
| CYGD | YML086c. |
| SGD | S000004551. ALO1. |
Phylogenomic databases | |
| HOGENOM | P54783. |
| OMA | DCLFSQF. |
Enzyme and pathway databases | |
| BRENDA | 1.1.3.37. 250. |
Gene expression databases | |
| ArrayExpress | P54783. |
| Genevestigator | P54783. |
| GermOnline | YML086C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR007173. ALO. IPR016166. FAD-bd_2. IPR010031. FAD_lactone_oxidase. IPR006094. Oxid_FAD_bind_N. IPR006093. Oxy_OxRdtase_FAD_BS. [Graphical view] |
| Pfam | PF04030. ALO. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01678. FAD_lactone_ox. 1 hit. |
| PROSITE | PS51387. FAD_PCMH. 1 hit. PS00862. OX2_COVAL_FAD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 977843. |
Entry information
| Entry name | ALO_YEAST | ||||||||
| Accession | Primary (citable) accession number: P54783 Secondary accession number(s): O42618 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIII Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names |
