Reviewed, UniProtKB/Swiss-Prot P54783 (ALO_YEAST)

Names and origin

Protein names

Recommended name:
    D-arabinono-1,4-lactone oxidase
      Short name=ALO
    EC=1.1.3.37
Alternative name(s):
    L-galactono-gamma-lactone oxidase

Gene names

Name:	ALO1
Ordered Locus Names:	YML086C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can oxidize L-gulono-1,4-lactone as well as D-arabinono-1,4-lactone and L-galactono-1,4-lactone.
Catalytic activity	D-arabinono-1,4-lactone + O(2) = dehydro-D-arabinono-1,4-lactone + H(2)O(2).
Cofactor	FAD.
Pathway	Cofactor biosynthesis; D-erythroascorbic acid biosynthesis; D-erythro-ascorbic acid from D-arabinose: step 2/2.
Subcellular location	Mitochondrion membrane. Note= Membrane-embedded.
Post-translational modification	The N-terminus is blocked.
Miscellaneous	Present with 6190 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the oxygen-dependent FAD-linked oxidoreductase family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to mitochondrial outer membrane Inferred from direct assay. Source: SGD
Molecular function	D-arabinono-1,4-lactone oxidase activity Ref.1 Inferred from electronic annotation. Source: InterPro FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	D-arabinono-1,4-lactone oxidase		PRO_0000128171
Regions
	Domain	19 – 193	175	FAD-binding PCMH-type
Amino acid modifications
	Modified residue	56	1	Tele-8alpha-FAD histidine By similarity
Experimental info
	Sequence conflict	417	1	A → P in BAA23804. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P54783-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 942177A74A738EC8	FASTA	526	59,494
	10 20 30 40 50 60 MSTIPFRKNY VFKNWAGIYS AKPERYFQPS SIDEVVELVK SARLAEKSLV TVGSGHSPSN 70 80 90 100 110 120 MCVTDEWLVN LDRLDKVQKF VEYPELHYAD VTVDAGMRLY QLNEFLGAKG YSIQNLGSIS 130 140 150 160 170 180 EQSVAGIIST GSHGSSPYHG LISSQYVNLT IVNGKGELKF LDAENDPEVF KAALLSVGKI 190 200 210 220 230 240 GIIVSATIRV VPGFNIKSTQ EVITFENLLK QWDTLWTSSE FIRVWWYPYT RKCVLWRGNK 250 260 270 280 290 300 TTDAQNGPAK SWWGTKLGRF FYETLLWIST KIYAPLTPFV EKFVFNRQYG KLEKSSTGDV 310 320 330 340 350 360 NVTDSISGFN MDCLFSQFVD EWGCPMDNGL EVLRSLDHSI AQAAINKEFY VHVPMEVRCS 370 380 390 400 410 420 NTTLPSEPLD TSKRTNTSPG PVYGNVCRPF LDNTPSHCRF APLENVTNSQ LTLYINATIY 430 440 450 460 470 480 RPFGCNTPIH KWFTLFENTM MVAGGKPHWA KNFLGSTTLA AGPVKKDTDY DDFEMRGMAL 490 500 510 520 KVEEWYGEDL KKFRKIRKEQ DPDNVFLANK QWAIINGIID PSELSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"D-erythroascorbic acid is an important antioxidant molecule in Saccharomyces cerevisiae." Huh W.-K., Lee B.-H., Kim S.-T., Kim Y.-R., Rhie G.-E., Baek Y.-W., Hwang C.-S., Lee J.-S., Kang S.-O. Mol. Microbiol. 30:895-903(1998) [PubMed: 10094636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 86-105 AND 349-363. Strain: ATCC 44774 / DBY747.
[2]	"Identification of the yeast genomic sequence encoding L-galactono-gamma-lactone oxidase." Nishikimi M., Ohata Y., Ishikawa T. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40390 Genomic DNA. Translation: AAC98538.1. AB009401 Genomic DNA. Translation: BAA23804.1. Z46660 Genomic DNA. Translation: CAA86652.1. AY693120 Genomic DNA. Translation: AAT93139.1.
PIR	S49641.
RefSeq	NP_013624.1.
3D structure databases
ModBase	Search...
Proteomic databases
PeptideAtlas	P54783.
Genome annotation databases
Ensembl	YML086C. Saccharomyces cerevisiae. [Contig view]
GeneID	854888.
GenomeReviews	Gene locus YML086C in contig Z71257_GR.
KEGG	sce:YML086C.
NMPDR	fig|4932.3.peg.4661.
Organism-specific databases
CYGD	YML086c.
SGD	S000004551. ALO1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P54783.
Gene expression databases
GermOnline	YML086C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007173. ALO. IPR010031. FAD_lactone_oxidase. IPR006094. Oxid_FAD_bind_N. IPR006093. Oxy_OxRdtase_FAD_BS. [Graphical view]
Pfam	PF04030. ALO. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR01678. FAD_lactone_ox. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. PS00862. OX2_COVAL_FAD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P54783.
NextBio	977843.

Entry information

Entry name

ALO_YEAST

Accession

Primary (citable) accession number: P54783
Secondary accession number(s): O42618

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)