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P54783

- ALO_YEAST

UniProt

P54783 - ALO_YEAST

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Protein

D-arabinono-1,4-lactone oxidase

Gene

ALO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Can oxidize L-gulono-1,4-lactone as well as D-arabinono-1,4-lactone and L-galactono-1,4-lactone.

Catalytic activityi

D-arabinono-1,4-lactone + O2 = dehydro-D-arabinono-1,4-lactone + H2O2.

Cofactori

Pathwayi

GO - Molecular functioni

  1. D-arabinono-1,4-lactone oxidase activity Source: SGD
  2. flavin adenine dinucleotide binding Source: InterPro
  3. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro

GO - Biological processi

  1. cellular response to oxidative stress Source: SGD
  2. dehydro-D-arabinono-1,4-lactone biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:YML086C-MONOMER.
YEAST:YML086C-MONOMER.
UniPathwayiUPA00771; UER00766.

Names & Taxonomyi

Protein namesi
Recommended name:
D-arabinono-1,4-lactone oxidase (EC:1.1.3.37)
Short name:
ALO
Alternative name(s):
L-galactono-gamma-lactone oxidase
Gene namesi
Name:ALO1
Ordered Locus Names:YML086C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML086c.
SGDiS000004551. ALO1.

Subcellular locationi

Mitochondrion membrane
Note: Membrane-embedded.

GO - Cellular componenti

  1. integral component of mitochondrial outer membrane Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526D-arabinono-1,4-lactone oxidasePRO_0000128171Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561Pros-8alpha-FAD histidineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiP54783.
PaxDbiP54783.
PeptideAtlasiP54783.

Expressioni

Gene expression databases

GenevestigatoriP54783.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-2519,EBI-2519
ACB1P317871EBI-2519,EBI-2060
ADE17P380091EBI-2519,EBI-14223
ARF1P110761EBI-2519,EBI-2816
ARO8P530901EBI-2519,EBI-2042933
CWP2P434971EBI-2519,EBI-2044078
CYS4P325821EBI-2519,EBI-4167
DAP1Q120911EBI-2519,EBI-5554
DBP5P204491EBI-2519,EBI-5617
DNM1P548611EBI-2519,EBI-6002
EHT1P382951EBI-2519,EBI-20890
FAA3P390021EBI-2519,EBI-10089
FUN14P184111EBI-2519,EBI-20609
GLN1P322881EBI-2519,EBI-7665
GTT1P405821EBI-2519,EBI-7941
HSP12P229431EBI-2519,EBI-8548
HUG1Q6Q5K61EBI-2519,EBI-392766
HYR1P405811EBI-2519,EBI-7869
IML2P470311EBI-2519,EBI-25966
INO1P119861EBI-2519,EBI-9257
IPP1P008171EBI-2519,EBI-9338
KES1P358441EBI-2519,EBI-9648
LSP1Q122301EBI-2519,EBI-34978
MCR1P360601EBI-2519,EBI-10565
MSP1P287371EBI-2519,EBI-11435
OYE2Q035581EBI-2519,EBI-12729
PCK1P109631EBI-2519,EBI-13770
PGM2P370121EBI-2519,EBI-13296
PMP3P872841EBI-2519,EBI-13555
QCR9P222891EBI-2519,EBI-19947
RNR1P215241EBI-2519,EBI-15234
RNR2P099381EBI-2519,EBI-15240
SAH1P399541EBI-2519,EBI-16451
SAM35P146931EBI-2519,EBI-24602
SEC4P075601EBI-2519,EBI-16858
SIS1P252941EBI-2519,EBI-17244
SNC2P333281EBI-2519,EBI-17512
SNF7P399291EBI-2519,EBI-17554
SSL2Q005781EBI-2519,EBI-14683
TOM20P351801EBI-2519,EBI-12522
TOM70P072131EBI-2519,EBI-12551
TSC10P383421EBI-2519,EBI-21124
UIP4Q089261EBI-2519,EBI-2044051
URA7P282741EBI-2519,EBI-20128
VAS1P07806-11EBI-2519,EBI-1009942From a different organism.
YBL039W-BP0C2681EBI-2519,EBI-2044092
YDR381C-AQ3E6R51EBI-2519,EBI-2044020
YGR012WP532061EBI-2519,EBI-2044073
YHR003CP387561EBI-2519,EBI-24431
YHR146WP388451EBI-2519,EBI-24770
YPT1P011231EBI-2519,EBI-29496
YPT31P385551EBI-2519,EBI-29379
YPT32P519961EBI-2519,EBI-29384

Protein-protein interaction databases

BioGridi35055. 53 interactions.
IntActiP54783. 39 interactions.
MINTiMINT-4497062.

Structurei

3D structure databases

ProteinModelPortaliP54783.
SMRiP54783. Positions 14-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 193175FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0277.
GeneTreeiENSGT00510000049722.
HOGENOMiHOG000204635.
InParanoidiP54783.
KOiK00107.
OMAiMDCLFSQ.
OrthoDBiEOG7JX3CZ.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR007173. ALO.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR010031. FAD_lactone_oxidase.
IPR023595. LGO_GLO.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
[Graphical view]
PfamiPF04030. ALO. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
PIRSFiPIRSF000136. LGO_GLO. 1 hit.
SUPFAMiSSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR01678. FAD_lactone_ox. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54783-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTIPFRKNY VFKNWAGIYS AKPERYFQPS SIDEVVELVK SARLAEKSLV
60 70 80 90 100
TVGSGHSPSN MCVTDEWLVN LDRLDKVQKF VEYPELHYAD VTVDAGMRLY
110 120 130 140 150
QLNEFLGAKG YSIQNLGSIS EQSVAGIIST GSHGSSPYHG LISSQYVNLT
160 170 180 190 200
IVNGKGELKF LDAENDPEVF KAALLSVGKI GIIVSATIRV VPGFNIKSTQ
210 220 230 240 250
EVITFENLLK QWDTLWTSSE FIRVWWYPYT RKCVLWRGNK TTDAQNGPAK
260 270 280 290 300
SWWGTKLGRF FYETLLWIST KIYAPLTPFV EKFVFNRQYG KLEKSSTGDV
310 320 330 340 350
NVTDSISGFN MDCLFSQFVD EWGCPMDNGL EVLRSLDHSI AQAAINKEFY
360 370 380 390 400
VHVPMEVRCS NTTLPSEPLD TSKRTNTSPG PVYGNVCRPF LDNTPSHCRF
410 420 430 440 450
APLENVTNSQ LTLYINATIY RPFGCNTPIH KWFTLFENTM MVAGGKPHWA
460 470 480 490 500
KNFLGSTTLA AGPVKKDTDY DDFEMRGMAL KVEEWYGEDL KKFRKIRKEQ
510 520
DPDNVFLANK QWAIINGIID PSELSD
Length:526
Mass (Da):59,494
Last modified:October 1, 1996 - v1
Checksum:i942177A74A738EC8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti417 – 4171A → P in BAA23804. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40390 Genomic DNA. Translation: AAC98538.1.
AB009401 Genomic DNA. Translation: BAA23804.1.
Z46660 Genomic DNA. Translation: CAA86652.1.
AY693120 Genomic DNA. Translation: AAT93139.1.
BK006946 Genomic DNA. Translation: DAA09811.1.
PIRiS49641.
RefSeqiNP_013624.1. NM_001182445.1.

Genome annotation databases

EnsemblFungiiYML086C; YML086C; YML086C.
GeneIDi854888.
KEGGisce:YML086C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40390 Genomic DNA. Translation: AAC98538.1 .
AB009401 Genomic DNA. Translation: BAA23804.1 .
Z46660 Genomic DNA. Translation: CAA86652.1 .
AY693120 Genomic DNA. Translation: AAT93139.1 .
BK006946 Genomic DNA. Translation: DAA09811.1 .
PIRi S49641.
RefSeqi NP_013624.1. NM_001182445.1.

3D structure databases

ProteinModelPortali P54783.
SMRi P54783. Positions 14-204.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35055. 53 interactions.
IntActi P54783. 39 interactions.
MINTi MINT-4497062.

Proteomic databases

MaxQBi P54783.
PaxDbi P54783.
PeptideAtlasi P54783.

Protocols and materials databases

DNASUi 854888.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML086C ; YML086C ; YML086C .
GeneIDi 854888.
KEGGi sce:YML086C.

Organism-specific databases

CYGDi YML086c.
SGDi S000004551. ALO1.

Phylogenomic databases

eggNOGi COG0277.
GeneTreei ENSGT00510000049722.
HOGENOMi HOG000204635.
InParanoidi P54783.
KOi K00107.
OMAi MDCLFSQ.
OrthoDBi EOG7JX3CZ.

Enzyme and pathway databases

UniPathwayi UPA00771 ; UER00766 .
BioCyci MetaCyc:YML086C-MONOMER.
YEAST:YML086C-MONOMER.

Miscellaneous databases

NextBioi 977843.
PROi P54783.

Gene expression databases

Genevestigatori P54783.

Family and domain databases

Gene3Di 3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProi IPR007173. ALO.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR010031. FAD_lactone_oxidase.
IPR023595. LGO_GLO.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
[Graphical view ]
Pfami PF04030. ALO. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view ]
PIRSFi PIRSF000136. LGO_GLO. 1 hit.
SUPFAMi SSF56176. SSF56176. 1 hit.
TIGRFAMsi TIGR01678. FAD_lactone_ox. 1 hit.
PROSITEi PS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "D-erythroascorbic acid is an important antioxidant molecule in Saccharomyces cerevisiae."
    Huh W.-K., Lee B.-H., Kim S.-T., Kim Y.-R., Rhie G.-E., Baek Y.-W., Hwang C.-S., Lee J.-S., Kang S.-O.
    Mol. Microbiol. 30:895-903(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 86-105 AND 349-363.
    Strain: ATCC 44774 / DBY747.
  2. "Identification of the yeast genomic sequence encoding L-galactono-gamma-lactone oxidase."
    Nishikimi M., Ohata Y., Ishikawa T.
    Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALO_YEAST
AccessioniPrimary (citable) accession number: P54783
Secondary accession number(s): D6W0J7, O42618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6190 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3