P54781 (ERG5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 61
EC=1.14.-.-

Alternative name(s):
C-22 sterol desaturase

Gene names

Name:	ERG5
Synonyms:	CYP61
Ordered Locus Names:	YMR015C
ORF Names:	YM9711.02C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required to form the C-22(23) double bond in the sterol side chain.
Cofactor	Heme group By similarity.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 4/5.
Subunit structure	Interacts with ERG28. Ref.8
Miscellaneous	Present with 24700 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ergosterol biosynthetic process Inferred from direct assay Ref.5. Source: SGD
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: SGD
Molecular function	C-22 sterol desaturase activity Inferred from direct assay Ref.5. Source: SGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Cytochrome P450 61		PRO_0000052050
Sites
	Metal binding	476	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Cross-link	164		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7
	Cross-link	198		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7
Experimental info
	Sequence conflict	286	1	I → T in AAU09769. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P54781 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2CE8DC6CE0315AC3	FASTA	538	61,334
	10 20 30 40 50 60 MSSVAENIIQ HATHNSTLHQ LAKDQPSVGV TTAFSILDTL KSMSYLKIFA TLICILLVWD 70 80 90 100 110 120 QVAYQIKKGS IAGPKFKFWP IIGPFLESLD PKFEEYKAKW ASGPLSCVSI FHKFVVIAST 130 140 150 160 170 180 RDLARKILQS SKFVKPCVVD VAVKILRPCN WVFLDGKAHT DYRKSLNGLF TKQALAQYLP 190 200 210 220 230 240 SLEQIMDKYM DKFVRLSKEN NYEPQVFFHE MREILCALSL NSFCGNYITE DQVRKIADDY 250 260 270 280 290 300 YLVTAALELV NFPIIIPYTK TWYGKKTADM AMKIFENCAQ MAKDHIAAGG KPVCVMDAWC 310 320 330 340 350 360 KLMHDAKNSN DDDSRIYHRE FTNKEISEAV FTFLFASQDA SSSLACWLFQ IVADRPDVLA 370 380 390 400 410 420 KIREEQLAVR NNDMSTELNL DLIEKMKYTN MVIKETLRYR PPVLMVPYVV KKNFPVSPNY 430 440 450 460 470 480 TAPKGAMLIP TLYPALHDPE VYENPDEFIP ERWVEGSKAS EAKKNWLVFG CGPHVCLGQT 490 500 510 520 530 YVMITFAALL GKFALYTDFH HTVTPLSEKI KVFATIFPKD DLLLTFKKRD PITGEVFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis." Skaggs B.A., Alexander J.F., Pierson C.A., Schweitzer K.S., Chun K.T., Koegel C., Barbuch R., Bard M. Gene 169:105-109(1996) [PubMed: 8635732] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase." Kelly S.L., Lamb D.C., Corran A.J., Baldwin B.C., Parks L.W., Kelly D.E. FEBS Lett. 377:217-220(1995) [PubMed: 8543054] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-42. Strain: DK2.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-164 AND LYS-198, MASS SPECTROMETRY. Strain: SUB592.
[8]	"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex." Mo C., Bard M. J. Lipid Res. 46:1991-1998(2005) [PubMed: 15995173] [Abstract] Cited for: INTERACTION WITH ERG28.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34636 Genomic DNA. Translation: AAB06217.1. Z49211 Genomic DNA. Translation: CAA89116.1. AY723852 Genomic DNA. Translation: AAU09769.1. BK006946 Genomic DNA. Translation: DAA09913.1.
PIR	S54015.
RefSeq	NP_013728.1. NM_001182511.1.
3D structure databases
ProteinModelPortal	P54781.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54781. 31 interactions.
MINT	MINT-1323986.
STRING	P54781.
Proteomic databases
PeptideAtlas	P54781.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR015C; YMR015C; YMR015C.
GeneID	855029.
KEGG	sce:YMR015C.
NMPDR	fig|4932.3.peg.4774.
Organism-specific databases
CYGD	YMR015c.
SGD	S000004617. ERG5.
Phylogenomic databases
eggNOG	fuNOG05691.
HOGENOM	HBG592092.
OMA	PLSCVSI.
OrthoDB	EOG4H49C4.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER3O-232.
Gene expression databases
ArrayExpress	P54781.
Genevestigator	P54781.
GermOnline	YMR015C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K09831.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978227.

Entry information

Entry name

ERG5_YEAST

Accession

Primary (citable) accession number: P54781
Secondary accession number(s): D6VZI9, E9P965

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program