Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome P450 61

Gene

ERG5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required to form the C-22(23) double bond in the sterol side chain.2 Publications

Catalytic activityi

Ergosta-5,7,24(28)-trien-3-beta-ol + NADPH + O2 = ergosta-5,7,22,24(28)-tetraen-3-beta-ol + NADP+ + 2 H2O.2 Publications

Cofactori

hemeBy similarity

Pathwayi: ergosterol biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes ergosterol from zymosterol.1 Publication
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Sterol 24-C-methyltransferase (ERG6)
  2. C-8 sterol isomerase (ERG2)
  3. Delta(7)-sterol 5(6)-desaturase (ERG3)
  4. Cytochrome P450 61 (ERG5)
  5. Delta(24(24(1)))-sterol reductase (ERG4)
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi476 – 4761Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • C-22 sterol desaturase activity Source: SGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • monooxygenase activity Source: InterPro

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-232.
YEAST:MONOMER3O-232.
UniPathwayiUPA00768; UER00763.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 611 Publication (EC:1.14.19.412 Publications)
Alternative name(s):
C-22 sterol desaturase1 Publication
Gene namesi
Name:ERG51 Publication
Synonyms:CYP611 Publication
Ordered Locus Names:YMR015CImported
ORF Names:YM9711.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR015C.
SGDiS000004617. ERG5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Cytochrome P450 61PRO_0000052050Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki164 – 164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki198 – 198Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP54781.
PeptideAtlasiP54781.

Interactioni

Subunit structurei

Interacts with ERG28.1 Publication

Protein-protein interaction databases

BioGridi35186. 155 interactions.
IntActiP54781. 16 interactions.
MINTiMINT-1323986.

Structurei

3D structure databases

ProteinModelPortaliP54781.
SMRiP54781. Positions 80-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

GeneTreeiENSGT00830000129055.
HOGENOMiHOG000181358.
InParanoidiP54781.
KOiK09831.
OMAiKDEEISC.
OrthoDBiEOG7Z69N7.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54781-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVAENIIQ HATHNSTLHQ LAKDQPSVGV TTAFSILDTL KSMSYLKIFA
60 70 80 90 100
TLICILLVWD QVAYQIKKGS IAGPKFKFWP IIGPFLESLD PKFEEYKAKW
110 120 130 140 150
ASGPLSCVSI FHKFVVIAST RDLARKILQS SKFVKPCVVD VAVKILRPCN
160 170 180 190 200
WVFLDGKAHT DYRKSLNGLF TKQALAQYLP SLEQIMDKYM DKFVRLSKEN
210 220 230 240 250
NYEPQVFFHE MREILCALSL NSFCGNYITE DQVRKIADDY YLVTAALELV
260 270 280 290 300
NFPIIIPYTK TWYGKKTADM AMKIFENCAQ MAKDHIAAGG KPVCVMDAWC
310 320 330 340 350
KLMHDAKNSN DDDSRIYHRE FTNKEISEAV FTFLFASQDA SSSLACWLFQ
360 370 380 390 400
IVADRPDVLA KIREEQLAVR NNDMSTELNL DLIEKMKYTN MVIKETLRYR
410 420 430 440 450
PPVLMVPYVV KKNFPVSPNY TAPKGAMLIP TLYPALHDPE VYENPDEFIP
460 470 480 490 500
ERWVEGSKAS EAKKNWLVFG CGPHVCLGQT YVMITFAALL GKFALYTDFH
510 520 530
HTVTPLSEKI KVFATIFPKD DLLLTFKKRD PITGEVFE
Length:538
Mass (Da):61,334
Last modified:October 1, 1996 - v1
Checksum:i2CE8DC6CE0315AC3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti286 – 2861I → T in AAU09769 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34636 Genomic DNA. Translation: AAB06217.1.
Z49211 Genomic DNA. Translation: CAA89116.1.
AY723852 Genomic DNA. Translation: AAU09769.1.
BK006946 Genomic DNA. Translation: DAA09913.1.
PIRiS54015.
RefSeqiNP_013728.1. NM_001182511.1.

Genome annotation databases

EnsemblFungiiYMR015C; YMR015C; YMR015C.
GeneIDi855029.
KEGGisce:YMR015C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34636 Genomic DNA. Translation: AAB06217.1.
Z49211 Genomic DNA. Translation: CAA89116.1.
AY723852 Genomic DNA. Translation: AAU09769.1.
BK006946 Genomic DNA. Translation: DAA09913.1.
PIRiS54015.
RefSeqiNP_013728.1. NM_001182511.1.

3D structure databases

ProteinModelPortaliP54781.
SMRiP54781. Positions 80-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35186. 155 interactions.
IntActiP54781. 16 interactions.
MINTiMINT-1323986.

Proteomic databases

MaxQBiP54781.
PeptideAtlasiP54781.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR015C; YMR015C; YMR015C.
GeneIDi855029.
KEGGisce:YMR015C.

Organism-specific databases

EuPathDBiFungiDB:YMR015C.
SGDiS000004617. ERG5.

Phylogenomic databases

GeneTreeiENSGT00830000129055.
HOGENOMiHOG000181358.
InParanoidiP54781.
KOiK09831.
OMAiKDEEISC.
OrthoDBiEOG7Z69N7.

Enzyme and pathway databases

UniPathwayiUPA00768; UER00763.
BioCyciMetaCyc:MONOMER3O-232.
YEAST:MONOMER3O-232.

Miscellaneous databases

PROiP54781.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis."
    Skaggs B.A., Alexander J.F., Pierson C.A., Schweitzer K.S., Chun K.T., Koegel C., Barbuch R., Bard M.
    Gene 169:105-109(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase."
    Kelly S.L., Lamb D.C., Corran A.J., Baldwin B.C., Parks L.W., Kelly D.E.
    FEBS Lett. 377:217-220(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 32-42, FUNCTION, CATALYTIC ACTIVITY.
    Strain: DK2.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-164 AND LYS-198.
    Strain: SUB592.
  8. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
    Mo C., Bard M.
    J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ERG28.

Entry informationi

Entry nameiERG5_YEAST
AccessioniPrimary (citable) accession number: P54781
Secondary accession number(s): D6VZI9, E9P965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 24700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.