Reviewed, UniProtKB/Swiss-Prot P54781 (ERG5_YEAST)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 61
    EC=1.14.14.-
Alternative name(s):
    C-22 sterol desaturase

Gene names

Name:	ERG5
Synonyms:	CYP61
Ordered Locus Names:	YMR015C
ORF Names:	YM9711.02C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required to form the C-22(23) double bond in the sterol side chain.
Cofactor	Heme group By similarity.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 4/5.
Subunit structure	Interacts with ERG28. Ref.6
Miscellaneous	Present with 24700 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q12164	1	EBI-6563,EBI-30000
AGP1	P25376	1	EBI-6563,EBI-2357
AYT1	Q12226	1	EBI-6563,EBI-2057459
CAN1	P04817	1	EBI-6563,EBI-3993
CHO1	P08456	1	EBI-6563,EBI-14055
ELO1	P39540	1	EBI-6563,EBI-2049096
ERG11	P10614	1	EBI-6563,EBI-5127
ERP1	Q05359	1	EBI-6563,EBI-6581
ERV25	P54837	1	EBI-6563,EBI-6642
GAP1	P19145	1	EBI-6563,EBI-7314
HXT3	P32466	1	EBI-6563,EBI-8770
HXT7	P39004	1	EBI-6563,EBI-8790
MYO1	P08964	1	EBI-6563,EBI-11650
OLE1	P21147	1	EBI-6563,EBI-2098
OST1	P41543	1	EBI-6563,EBI-12651
PHO84	P25297	1	EBI-6563,EBI-13320
PMP2	P40975	1	EBI-6563,EBI-2043041
PMP3	P87284	1	EBI-6563,EBI-13555
RTN1	Q04947	1	EBI-6563,EBI-38020
SAC1	P32368	1	EBI-6563,EBI-16210
SPF1	P39986	1	EBI-6563,EBI-3128
UBP1	P25037	1	EBI-6563,EBI-19819
VRG4	P40107	1	EBI-6563,EBI-7764
YER053C-A	Q3E7B0	1	EBI-6563,EBI-2047907

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Cytochrome P450 61		PRO_0000052050
Sites
	Metal binding	476	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Cross-link	164		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5
	Cross-link	198		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P54781-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2CE8DC6CE0315AC3	FASTA	538	61,334
	10 20 30 40 50 60 MSSVAENIIQ HATHNSTLHQ LAKDQPSVGV TTAFSILDTL KSMSYLKIFA TLICILLVWD 70 80 90 100 110 120 QVAYQIKKGS IAGPKFKFWP IIGPFLESLD PKFEEYKAKW ASGPLSCVSI FHKFVVIAST 130 140 150 160 170 180 RDLARKILQS SKFVKPCVVD VAVKILRPCN WVFLDGKAHT DYRKSLNGLF TKQALAQYLP 190 200 210 220 230 240 SLEQIMDKYM DKFVRLSKEN NYEPQVFFHE MREILCALSL NSFCGNYITE DQVRKIADDY 250 260 270 280 290 300 YLVTAALELV NFPIIIPYTK TWYGKKTADM AMKIFENCAQ MAKDHIAAGG KPVCVMDAWC 310 320 330 340 350 360 KLMHDAKNSN DDDSRIYHRE FTNKEISEAV FTFLFASQDA SSSLACWLFQ IVADRPDVLA 370 380 390 400 410 420 KIREEQLAVR NNDMSTELNL DLIEKMKYTN MVIKETLRYR PPVLMVPYVV KKNFPVSPNY 430 440 450 460 470 480 TAPKGAMLIP TLYPALHDPE VYENPDEFIP ERWVEGSKAS EAKKNWLVFG CGPHVCLGQT 490 500 510 520 530 YVMITFAALL GKFALYTDFH HTVTPLSEKI KVFATIFPKD DLLLTFKKRD PITGEVFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis." Skaggs B.A., Alexander J.F., Pierson C.A., Schweitzer K.S., Chun K.T., Koegel C., Barbuch R., Bard M. Gene 169:105-109(1996) [PubMed: 8635732] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase." Kelly S.L., Lamb D.C., Corran A.J., Baldwin B.C., Parks L.W., Kelly D.E. FEBS Lett. 377:217-220(1995) [PubMed: 8543054] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-42. Strain: DK2.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-164 AND LYS-198, MASS SPECTROMETRY.
[6]	"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex." Mo C., Bard M. J. Lipid Res. 46:1991-1998(2005) [PubMed: 15995173] [Abstract] Cited for: INTERACTION WITH ERG28.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U34636 Genomic DNA. Translation: AAB06217.1. Z49211 Genomic DNA. Translation: CAA89116.1.
PIR	S54015.
RefSeq	NP_013728.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P54781. 37 interactions.
Proteomic databases
PeptideAtlas	P54781.
Genome annotation databases
Ensembl	YMR015C. Saccharomyces cerevisiae. [Contig view]
GeneID	855029.
GenomeReviews	Gene locus YMR015C in contig Z71257_GR.
KEGG	sce:YMR015C.
NMPDR	fig|4932.3.peg.4774.
Organism-specific databases
CYGD	YMR015c.
SGD	S000004617. ERG5.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P54781.
OMA	P54781. IPERWVE.
Gene expression databases
ArrayExpress	P54781.
GermOnline	YMR015C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978227.

Entry information

Entry name

ERG5_YEAST

Accession

Primary (citable) accession number: P54781

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)