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Protein

Peroxisome assembly factor 2

Gene

Pex6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in peroxisome biosynthesis. Required for stability of the PTS1 receptor. Probably required for protein import into peroxisomes. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi470 – 4778ATPSequence analysis
Nucleotide bindingi742 – 7498ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • peroxisome organization Source: RGD
  • protein import into peroxisome matrix, translocation Source: Ensembl
  • protein stabilization Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Peroxisome biogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisome assembly factor 2
Short name:
PAF-2
Alternative name(s):
Peroxin-6
Peroxisomal biogenesis factor 6
Peroxisomal-type ATPase 1
Gene namesi
Name:Pex6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi621637. Pex6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • peroxisomal membrane Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi476 – 4761K → A: No loss of function. 1 Publication
Mutagenesisi748 – 7481K → A: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 978978Peroxisome assembly factor 2PRO_0000084609Add
BLAST

Proteomic databases

PaxDbiP54777.
PRIDEiP54777.

Expressioni

Gene expression databases

GenevisibleiP54777. RN.

Interactioni

Subunit structurei

Interacts directly with PEX26 and PEX1. Mediates the indirect interaction between PEX1 and PEX26. Interacts with ZFAND6 (By similarity).By similarity

Protein-protein interaction databases

BioGridi250712. 2 interactions.
STRINGi10116.ENSRNOP00000022582.

Structurei

3D structure databases

ProteinModelPortaliP54777.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0736. Eukaryota.
ENOG410XNT9. LUCA.
GeneTreeiENSGT00550000074953.
HOGENOMiHOG000241031.
HOVERGENiHBG002311.
InParanoidiP54777.
KOiK13339.
OMAiPSVSWHD.
OrthoDBiEOG74J979.
PhylomeDBiP54777.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALAVLHVLE PFPTETPPLA VLLPPGGPWP VTGVGLVLAL RPASESPAGP
60 70 80 90 100
ALLVAAVEGS GAQCEQRGPG PPPLLVSRTL LRVLALSPGA RVRARPVRRP
110 120 130 140 150
PALGWALLGT SPGPGLGPRV GPLLVRRGET LPVPGSRVLE TRPALQGLLG
160 170 180 190 200
PGTRLAVTEL QGRTKLDPES RDHNHPPPPP VVSSFAVSHS IRQLRGVLGG
210 220 230 240 250
TGDALGVSRS CLRSLGLFQG EWVWVARVGE LPNTSQPHLA QVQVLEPRWD
260 270 280 290 300
LSARLGPNSG QPGEPLADGL VFVPATLAFN LGCDPLEVGE LRIQRYLEDS
310 320 330 340 350
TAAEDKGSCS LLPGPPFARE LHIEVLPSPH CGVNGKYDHV LYQHFHTPRV
360 370 380 390 400
VQEGDVLCVS TAGQVEILEG SLERLPRWRE VFFKVKKTVG EAPDGPASAF
410 420 430 440 450
LADTTHTSLY LAGTTLSRVP PLPSGRSPPW DSLSPPGLEA LVNELCAVLK
460 470 480 490 500
PHLQPGGTLL TGTSCVLLQG PPGSGKTTAV TAACSRLGLH LLKVPCSSLC
510 520 530 540 550
ADSSRTVETK LQTTFSRARR CRPVVLLLTA LDLLGRDRDG LGEDARVVAT
560 570 580 590 600
LRHLLLDEDP LSRCPPLMVV ATTSRVQDLP TDVRTAFPHE LEVPVLSESQ
610 620 630 640 650
RLSVLQALTA HLPLGQEVNL SQLARRCAGF VVGDLYALLT HASRAACTRI
660 670 680 690 700
KAAGLAMSEE DEGELCAAGF PLLAEDFGQA LDQLQTAHSQ AVGAPKIPSV
710 720 730 740 750
SWHDVGGLQD VKKEILETIQ LPLEHPELLS LGLRRSGLLL HGPPGTGKTL
760 770 780 790 800
LAKAVATECS LTFLSVKGPE LINMYVGQSE ENVREVFARA RAAAPCIIFF
810 820 830 840 850
DELDSLAPSR GRSGDSGGVM DRVVSQLLAE LDGLHSTQDV FVIGATNRPD
860 870 880 890 900
LLDPALLRPG RFDKLVFVGA SEDRASQLRV LSAITRKFKL EASVSLMNVL
910 920 930 940 950
DCCPPQLTGA DLYSLCSDAM MTALKRRVRD LEEGLEPRSS ALLLTMEDLL
960 970
QAAARLQPSV SEQELLRYKR IQRKFAAC
Length:978
Mass (Da):104,426
Last modified:October 1, 1996 - v1
Checksum:iF723193B7E95EA97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 2991D → G in BAA24931 (Ref. 2) Curated
Sequence conflicti333 – 3331V → A in BAA24931 (Ref. 2) Curated
Sequence conflicti343 – 3431Q → R in BAA24931 (Ref. 2) Curated
Sequence conflicti546 – 5461R → C in BAA24931 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63673 mRNA. Translation: BAA09824.1.
D89660 Genomic DNA. Translation: BAA24931.1.
RefSeqiNP_476466.1. NM_057125.1.
UniGeneiRn.10675.

Genome annotation databases

EnsembliENSRNOT00000022582; ENSRNOP00000022582; ENSRNOG00000016655.
GeneIDi117265.
KEGGirno:117265.
UCSCiRGD:621637. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63673 mRNA. Translation: BAA09824.1.
D89660 Genomic DNA. Translation: BAA24931.1.
RefSeqiNP_476466.1. NM_057125.1.
UniGeneiRn.10675.

3D structure databases

ProteinModelPortaliP54777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250712. 2 interactions.
STRINGi10116.ENSRNOP00000022582.

Proteomic databases

PaxDbiP54777.
PRIDEiP54777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022582; ENSRNOP00000022582; ENSRNOG00000016655.
GeneIDi117265.
KEGGirno:117265.
UCSCiRGD:621637. rat.

Organism-specific databases

CTDi5190.
RGDi621637. Pex6.

Phylogenomic databases

eggNOGiKOG0736. Eukaryota.
ENOG410XNT9. LUCA.
GeneTreeiENSGT00550000074953.
HOGENOMiHOG000241031.
HOVERGENiHBG002311.
InParanoidiP54777.
KOiK13339.
OMAiPSVSWHD.
OrthoDBiEOG74J979.
PhylomeDBiP54777.

Miscellaneous databases

PROiP54777.

Gene expression databases

GenevisibleiP54777. RN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Peroxisome assembly factor-2, a putative ATPase cloned by functional complementation on a peroxisome-deficient mammalian cell mutant."
    Tsukamoto T., Miura S., Nakai T., Yokota S., Shimozawa N., Suzuki Y., Orii T., Fujiki Y., Sakai F., Bogaki A., Yasumo H., Osumi T.
    Nat. Genet. 11:395-401(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-476 AND LYS-748.
    Strain: Fischer 344.
    Tissue: Liver.
  2. Tsukamoto T., Hashiguchi N.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiPEX6_RAT
AccessioniPrimary (citable) accession number: P54777
Secondary accession number(s): O55097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.