P54767 (DCE_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutamate decarboxylase Short name=GAD EC=4.1.1.15 Alternative name(s): ERT D1
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the production of GABA. The calmodulin-binding is calcium-dependent and it is proposed that this may, directly or indirectly, form a calcium regulated control of GABA biosynthesis By similarity.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Pyridoxal phosphate.
Sequence similarities	Belongs to the group II decarboxylase family.

Ontologies

Keywords
Ligand	Calmodulin-binding Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutamate decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Glutamate decarboxylase		PRO_0000146975
Regions
	Region	471 – 502	32	Calmodulin-binding By similarity
Amino acid modifications
	Modified residue	278	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54767 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1C5F9BD0084272A6	FASTA	502	56,785
	10 20 30 40 50 60 MVLTTTSIRD SEESLHCTFA SRYVQEPLPK FKMPKKSMPK EAAYQIVNDE LMLDGNPRLN 70 80 90 100 110 120 LASFVSTWME PECDKLIMSS INKNYVDMDE YPVTTELQNR CVNMLAHLFH APVGDDETAV 130 140 150 160 170 180 GVGTVGSSEA IMLAGLAFKR KWQSKRKAEG KPFDKPNIVT GANVQVCWEK FARYFEVELK 190 200 210 220 230 240 EVKLKEGYYV MDPAKAVEIV DENTICVAAI LGSTLTGEFE DVKLLNELLT KKNKETGWET 250 260 270 280 290 300 PIHVDAASGG FIAPFLWPDL EWDFRLPLVK SINVSGHKYG LVYAGVGWVI WRSKEDLPDE 310 320 330 340 350 360 LVFHINYLGS DQPTFTLNFS KGSYQIIAQY YQLIRLGFEG YKNVMKNCLS NAKVLTEGIT 370 380 390 400 410 420 KMGRFDIVSK DVGVPVVAFS LRDSSKYTVF EVSEHLRRFG WIVPAYTMPP DAEHIAVLRV 430 440 450 460 470 480 VIREDFSHSL AERLVSDIEK ILSELDTQPP RLPTKAVRVT AEEVRDDKGD GLHHFHMDTV 490 500 ETQKDIIKHW RKIAGKKTSG VC

References

[1]

"A role for glutamate decarboxylase during tomato ripening: the characterisation of a cDNA encoding a putative glutamate decarboxylase with a calmodulin-binding site." Gallego P.P., Whotton L., Picton S., Grierson D., Gray J.E. Plant Mol. Biol. 27:1143-1151(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Ailsa Craig. Tissue: Pericarp.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80840 mRNA. Translation: CAA56812.1.
PIR	S56177.
RefSeq	NP_001234041.1. NM_001247112.1.
UniGene	Les.21402.
3D structure databases
ProteinModelPortal	P54767.
ModBase	Search...
Proteomic databases
ProMEX	P54767.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	544313.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15560.
Family and domain databases
Gene3D	3.40.640.10. 1 hit.
InterPro	IPR010107. Glutamate_decarboxylase. IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11999:SF1. PTHR11999:SF1. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01788. Glu-decarb-GAD. 1 hit.
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCE_SOLLC

Accession

Primary (citable) accession number: P54767

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program