Reviewed, UniProtKB/Swiss-Prot P54761 (EPHB4_MOUSE)

Names and origin

Protein names

Recommended name:
    Ephrin type-B receptor 4
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor MDK-2
    Developmental kinase 2
    Tyrosine kinase MYK-1

Gene names

Name:	Ephb4
Synonyms:	Htk, Mdk2, Myk1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	987 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for members of the ephrin-B family. Binds to ephrin-B2.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Developing cardiovascular system.
Developmental stage	Abundant expression at E16.5 in various organ systems, including thymus, heart, lungs and kidney, which appeared to be associated with cells of endothelial origin.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Chain	16 – 987	972	Ephrin type-B receptor 4		PRO_0000016835
Regions
	Topological domain	16 – 539	524	Extracellular Potential
	Transmembrane	540 – 560	21	Potential
	Topological domain	561 – 987	427	Cytoplasmic Potential
	Domain	323 – 428	106	Fibronectin type-III 1
	Domain	434 – 526	93	Fibronectin type-III 2
	Domain	615 – 899	285	Protein kinase
	Domain	907 – 971	65	SAM
	Nucleotide binding	621 – 629	9	ATP By similarity
	Motif	985 – 987	3	PDZ-binding Potential
	Compositional bias	184 – 320	137	Cys-rich
Sites
	Active site	740	1	Proton acceptor By similarity
	Binding site	647	1	ATP By similarity
Amino acid modifications
	Modified residue	769	1	Phosphoserine By similarity
	Modified residue	911	1	Phosphoserine By similarity
	Modified residue	953	1	Phosphothreonine By similarity
	Glycosylation	203	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	351 – 353	3	GRE → RPR in AAA18591. Ref.2
	Sequence conflict	389	1	P → R in AAA18591. Ref.2
	Sequence conflict	659	1	R → A in AAA18591. Ref.2
	Sequence conflict	783	1	S → P in AAA18591. Ref.2
	Sequence conflict	805	1	S → R in AAA18591. Ref.2
	Sequence conflict	913	1	G → V in AAA18591. Ref.2
	Sequence conflict	938	1	V → M in AAA18591. Ref.2
	Sequence conflict	970 – 971	2	SQ → WE in AAA18591. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P54761-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 471F809579D98A02	FASTA	987	108,923
	10 20 30 40 50 60 MELRALLCWA SLATALEETL LNTKLETADL KWVTYPQAEG QWEELSGLDE EQHSVRTYEV 70 80 90 100 110 120 CDMKRPGGQA HWLRTGWVPR RGAVHVYATI RFTMMECLSL PRASRSCKET FTVFYYESER 130 140 150 160 170 180 DTATAHTPAW MENPYIKVDT VAAEHLTRKR PGAEATGKVN IKTLRLGPLS KAGFYLAFQD 190 200 210 220 230 240 QGACMALLSL HLFYKKCSWL ITNLTYFPET VPRELVVPVA GSCVANAVPT ANPSPSLYCR 250 260 270 280 290 300 EDGQWAEQQV TGCSCAPGYE AAESNKVCRA CGQGTFKPQI GDESCLPCPA NSHSNNIGSP 310 320 330 340 350 360 VCLCRIGYYR ARSDPRSSPC TTPPSAPRSV VHHLNGSTLR LEWSAPLESG GREDLTYAVR 370 380 390 400 410 420 CRECRPGGSC LPCGGDMTFD PGPRDLVEPW VAIRGLRPDV TYTFEVAALN GVSTLATGPP 430 440 450 460 470 480 PFEPVNVTTD REVPPAVSDI RVTRSSPSSL ILSWAIPRAP SGAVLDYEVK YHEKGAEGPS 490 500 510 520 530 540 SVRFLKTSEN RAELRGLKRG ASYLVQVRAR SEAGYGPFGQ EHHSQTQLDE SESWREQLAL 550 560 570 580 590 600 IAGTAVVGVV LVLVVVIIAV LCLRKQSNGR EVEYSDKHGQ YLIGHGTKVY IDPFTYEDPN 610 620 630 640 650 660 EAVREFAKEI DVSYVKIEEV IGAGEFGEVC RGRLKAPGKK ESCVAIKTLK GGYTERQRRE 670 680 690 700 710 720 FLSEASIMGQ FEHPNIIRLE GVVTNSVPVM ILTEFMENGA LDSFLRLNDG QFTVIQLVGM 730 740 750 760 770 780 LRGIASGMRY LAEMSYVHRD LAARNILVNS NLVCKVSDFG LSRFLEENSS DPTYTSSLGG 790 800 810 820 830 840 KISIRWTAPE AIAFRKFTSA SDAWSYGIVM WEVMSFGERP YWDMSNQDVI NAIEQDYRLP 850 860 870 880 890 900 PPPDCPTSLH QLMLDCWQKD RNARPRFPQV VSALDKMIRN PASLKIVARE NGGASHPLLD 910 920 930 940 950 960 QRQPHYSAFG SVGEWLRAIK MGRYEESFAA AGFGSFEVVS QISAEDLLRI GVTLAGHQKK 970 980 ILASVQHMKS QAKPGAPGGT GGPAQQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization, and differential expression of MDK2 and MDK5, two novel receptor tyrosine kinases of the eck/eph family." Ciossek T., Lerch M.M., Ullrich A. Oncogene 11:2085-2095(1995) [PubMed: 7478528] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Kidney.
[2]	"Expression of two novel eph-related receptor protein tyrosine kinases in mammary gland development and carcinogenesis." Andres A.-C., Reid H.H., Zurcher G., Blaschke R.J., Albrecht D., Ziemiecki A. Oncogene 9:1461-1467(1994) [PubMed: 8152808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Lung.
[3]	"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-590 AND TYR-774, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49085 mRNA. Translation: CAA88909.1. U06834 mRNA. Translation: AAA18591.1.
IPI	IPI00758066.
PIR	I48652. I48953.
UniGene	Mm.34533
3D structure databases
HSSP	HSSP built from PDB template 1JPA based on UniProtKB P54763.
SMR	P54761. Positions 17-196, 588-885.
ModBase	Search...
PTM databases
PhosphoSite	P54761.
Proteomic databases
PRIDE	P54761.
Genome annotation databases
Ensembl	ENSMUSG00000029710. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:104757. Ephb4.
Phylogenomic databases
HOVERGEN	P54761.
Enzyme and pathway databases
BRENDA	2.7.10.1. 244.
Gene expression databases
ArrayExpress	P54761.
Bgee	P54761.
CleanEx	MM_EPHB4.
GermOnline	ENSMUSG00000029710. Mus musculus.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR011510. SAM_2. IPR013761. SAM_type. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016257. TyrPK_ephrin_receptor. IPR001426. YKase_receptorV_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF07714. Pkinase_Tyr. 1 hit. PF07647. SAM_2. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00109. TYRKINASE.
ProDom	PD001495. Ephrin_receptor. 1 hit. PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

EPHB4_MOUSE

Accession

Primary (citable) accession number: P54761
Secondary accession number(s): Q60627

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)