Reviewed, UniProtKB/Swiss-Prot P54758 (EPHA6_RAT)

Names and origin

Protein names

Recommended name:
    Ephrin type-A receptor 6
    EC=2.7.10.1
Alternative name(s):
    EPH homology kinase 2
      Short name=EHK-2

Gene names

Name:	Epha6
Synonyms:	Ehk-2, Ehk2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1035 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-A family.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Brain.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 1035	1013	Ephrin type-A receptor 6		PRO_0000016817
Regions
	Topological domain	23 – 549	527	Extracellular Potential
	Transmembrane	550 – 570	21	Potential
	Topological domain	571 – 1035	465	Cytoplasmic Potential
	Domain	330 – 434	105	Fibronectin type-III 1
	Domain	438 – 533	96	Fibronectin type-III 2
	Domain	630 – 943	314	Protein kinase
	Domain	960 – 1024	65	SAM
	Nucleotide binding	636 – 644	9	ATP By similarity
	Region	33 – 206	174	Ephrin-binding
	Motif	1033 – 1035	3	PDZ-binding Potential
	Compositional bias	193 – 327	135	Cys-rich
Sites
	Active site	797	1	Proton acceptor By similarity
	Binding site	662	1	ATP By similarity
Amino acid modifications
	Modified residue	605	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	611	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	830	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	977	1	Phosphotyrosine; by autocatalysis Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	396	1	N-linked (GlcNAc...) Potential
	Glycosylation	409	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P54758-1 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 5ADEDB20A8B5AD95	FASTA	1,035	116,209
	10 20 30 40 50 60 MGGCEVREFL LQFGFFLPLL TAWTGDCSHV SNQVVLLDTS TVMGELGWKT YPLNGWDAIT 70 80 90 100 110 120 EMDEHNRPIH TYQVCNVMEP NQNNWLRTNW ISRDAAQKIY VEMKFTLRDC NSIPWVLGTC 130 140 150 160 170 180 KETFTLYYIE SDESHGTKFK PSQYIKIDTI AADESFTQMD LGDRILKLNT EVREVGPIER 190 200 210 220 230 240 KGFYLAFQDI GACIALVSVR VFYKKCPFTV RNLAMFPDTI PRVDSSSLVE VRGSCVKSSE 250 260 270 280 290 300 ERDTPKLYCG ADGDWLVPLG RCICTTGYEE IEGSCHACRP GFYKAFAGNT KCSKCPPHSS 310 320 330 340 350 360 TFVEATSVCH CEKGYFRAEK DPPSMACTRP PSAPRNVAFN INETALILEW SPPSDTGGRK 370 380 390 400 410 420 DLTYSVICKK CGVDASQCED CGAGLRFIPR PTGLINNSVV VLDFVSHVNY TFEIEAMNGV 430 440 450 460 470 480 SELSISPKPF TAITVTTDQD APSLIGMMRK DWASQNSLAL SWQAPAFSNG AILDYEIKYY 490 500 510 520 530 540 EKEHEQLTYS STRSKAPSVI ITGLKPATTY IFHIRVRTAT GYSGYSQKFE FETGDETSDM 550 560 570 580 590 600 AAEQGQILVI ATAAVGGFTL LVILTLFFLI TGRCQWYIKA KMKSEEKRRT HLQNSHLRFP 610 620 630 640 650 660 GIKTYIDPDT YEDPSLAVHE FEKEIDPSRI RIERVIGAGE FGEVCSGRLK TPGKREIPVA 670 680 690 700 710 720 IKTLKGGHMD RQRRDFLREA SIMGQFDHPN IIRLEGVVTK RSFPAIGVEA FCPSFLRAGF 730 740 750 760 770 780 LNGIQAPHPV TAGGSLPPRI PAGRPVMIVV EYMENGSLDS FLRKHDGHFT VIQLVGMLRG 790 800 810 820 830 840 IASGMKYLSD MGYVHRDLAA RNILVNSNLV CKVSDFGLSR VLEDDPEAAY TTTGGKIPIR 850 860 870 880 890 900 WTAPEAIAYR KFSSASDVWS YGIVMWEVMS YGERPYWEMS NQDVILSIEE GYRLPAPMGC 910 920 930 940 950 960 PPSLHQLMLH CWQKERNHRP KFTDIVSFLD KLIRNPSALH TLVEDILVMP ESPGDVPEYP 970 980 990 1000 1010 1020 LFVTVGDWLD SIKMGQYKSN FMAAGFTTFD LISRMSIEDI RRIGVILIGH QRRIVSSIQT 1030 LRLHMMHIQE KGFHV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"Ehk-1 and Ehk-2: two novel members of the Eph receptor-like tyrosine kinase family with distinctive structures and neuronal expression." Maisonpierre P.C., Barrezueta N.X., Yancopoulos G.D. Oncogene 8:3277-3288(1993) [PubMed: 7504232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-948. Strain: Sprague-Dawley. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABR03078339 Genomic DNA. No translation available. AABR03079251 Genomic DNA. No translation available. AABR03078729 Genomic DNA. No translation available. AABR03078861 Genomic DNA. No translation available. AABR03078320 Genomic DNA. No translation available. AABR03078411 Genomic DNA. No translation available. AABR03078368 Genomic DNA. No translation available. AABR03080204 Genomic DNA. No translation available. AABR03000001 Genomic DNA. No translation available.
IPI	IPI00763168.
PIR	S51605.
UniGene	Rn.102933
3D structure databases
SMR	P54758. Positions 33-207, 329-533, 451-535, 617-937, 957-1029.
ModBase	Search...
Protein-protein interaction databases
STRING	P54758.
PTM databases
PhosphoSite	P54758.
Genome annotation databases
Ensembl	ENSRNOT00000047310; ENSRNOP00000049409; ENSRNOG00000029184; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	1304614. Epha6.
Phylogenomic databases
eggNOG	roNOG06939.
HOVERGEN	P54758.
InParanoid	P54758.
Enzyme and pathway databases
BRENDA	2.7.10.1. 248.
Gene expression databases
Genevestigator	P54758.
Family and domain databases
InterPro	IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR008979. Galactose-bd-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR010993. SAM_homology. IPR021129. SAM_type1. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR016257. Tyr_prot_kinase_ephrin_rcpt. IPR001426. Tyr_prot_kinase_rcpt_V_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF00536. SAM_1. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII.
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPHA6_RAT

Accession

Primary (citable) accession number: P54758

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 16, 2006
Last modified:	February 9, 2010
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)