P54755 (EPHA5_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ephrin type-A receptor 5
EC=2.7.10.1

Alternative name(s):
EPH-like kinase 7
Short name=EK7
Short name=cEK7

Gene names

Name:	EPHA5
Synonyms:	CEK7

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1013 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses By similarity.
Subcellular location	Cell membrane By similarity; Single-pass type I membrane protein By similarity. Cell projection › axon By similarity. Cell projection › dendrite By similarity.
Tissue specificity	Detected in the 10-day embryonic brain, weaker expression in the rest of the 10-day embryo. Undetected in adult tissues.
Post-translational modification	Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 1 Eph LBD (Eph ligand-binding) domain. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Biological process	Neurogenesis
Cellular component	Cell membrane Cell projection Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axon guidance Inferred from sequence or structural similarity. Source: UniProtKB hippocampus development Inferred from sequence or structural similarity. Source: UniProtKB regulation of Rac GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of actin cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	axon Inferred from electronic annotation. Source: UniProtKB-SubCell dendrite Inferred from sequence or structural similarity. Source: UniProtKB integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GPI-linked ephrin receptor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 3 (identifier: P54755-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: P54755-2)

The sequence of this isoform differs from the canonical sequence as follows:
     573-595: SCCDHGCGWASSLRAVAYPSLIW → R

Isoform 2 (identifier: P54755-3)

The sequence of this isoform differs from the canonical sequence as follows:
     280-443: Missing.
     573-595: SCCDHGCGWASSLRAVAYPSLIW → R

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 1013	982	Ephrin type-A receptor 5		PRO_0000016815
Regions
	Topological domain	32 – 549	518	Extracellular Potential
	Transmembrane	550 – 570	21	Helical; Potential
	Topological domain	571 – 1013	443	Cytoplasmic Potential
	Domain	36 – 214	179	Eph LBD
	Domain	333 – 435	103	Fibronectin type-III 1
	Domain	445 – 535	91	Fibronectin type-III 2
	Domain	651 – 912	262	Protein kinase
	Domain	941 – 1013	73	SAM
	Nucleotide binding	657 – 665	9	ATP By similarity
	Motif	1011 – 1013	3	PDZ-binding Potential
	Compositional bias	196 – 330	135	Cys-rich
Sites
	Active site	776	1	Proton acceptor By similarity
	Binding site	683	1	ATP By similarity
Amino acid modifications
	Modified residue	626	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	632	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	809	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	958	1	Phosphotyrosine; by autocatalysis By similarity
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	275	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	399	1	N-linked (GlcNAc...) Potential
	Glycosylation	412	1	N-linked (GlcNAc...) Potential
	Glycosylation	437	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	280 – 443	164	Missing in isoform 2.		VSP_003004
	Alternative sequence	573 – 595	23	SCCDH…PSLIW → R in isoform 1 and isoform 2.		VSP_003005
Experimental info
	Sequence conflict	981 – 1013	33	LRRLG…GMVPL → ESPCEKWSLTLHPLFPTGYQ T in CAA79508. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: AC36FD6AEBF38382	FASTA	1,013	112,246
	10 20 30 40 50 60 MGLRGGGGRA GGPAPGWTCL LLCAALRSLL ASPGSEVNLL DSRTVMGDLG WIAYPKNGWE 70 80 90 100 110 120 EIGEVDENYA PIHTYQVCKV MEQNQNNWLL TSWISNEGRP ASSFELKFTL RDCNSLPGGL 130 140 150 160 170 180 GTCKETFNMY YFESDDEDGR NIRENQYIKI DTIAADESFT ELDLGDRVMK LNTEVRDVGP 190 200 210 220 230 240 LTKKGFYLAF QDVGACIALV SVRVYYKKCP SVIRNLARFP DTITGADSSQ LLEVSGVCVN 250 260 270 280 290 300 HSVTDEAPKM HCSAEGEWLV PIGKCLCKAG YEEKNNTCQV CRPGFFKASP HSPSCSKCPP 310 320 330 340 350 360 HSYTLDEAST SCLCEEHYFR RESDPPTMAC TRPPSAPRSA ISNVNETSVF LEWIPPADTG 370 380 390 400 410 420 GRKDVSYYIA CKKCNSHSGL CEACGSHVRY LPQQTGLKNT SVMMVDLLAH TNYTFEIEAV 430 440 450 460 470 480 NGVSDQNPGA RQFVSVNVTT NQAAPSPVSS VKKGKITKNS ISLSWQEPDR PNGIILEYEI 490 500 510 520 530 540 KYFEKDQETS YTIIKSKETA ITADGLKPGS AYVFQIRART AAGYGGFSRR FEFETSPVLA 550 560 570 580 590 600 ASSDQSQIPI IVVSVTVGVI LLAVVIGFLL SGSCCDHGCG WASSLRAVAY PSLIWRCGYS 610 620 630 640 650 660 KAKQDPEEEK MHFHNGHIKL PGVRTYIDPH TYEDPNQAVH EFAKEIEASC ITIERVIGAG 670 680 690 700 710 720 EFGEVCSGRL KLQGKREFPV AIKTLKVGYT EKQRRDFLGE ASIMGQFDHP NIIHLEGVVT 730 740 750 760 770 780 KSKPVMIVTE YMENGSLDTF LKKNDGQFTV IQLVGMLRGI ASGMKYLSDM GYVHRDLAAR 790 800 810 820 830 840 NILINSNLVC KVSDFGLSRV LEDDPEAAYT TRGGKIPIRW TAPEAIAFRK FTSASDVWSY 850 860 870 880 890 900 GIVMWEVMSY GERPYWEMTN QDVIKAVEEG YRLPSPMDCP AALYQLMLDC WQKDRNSRPK 910 920 930 940 950 960 FDEIVSMLDK LIRNPSSLKT LVNASSRVSN LLVEHSPVGS GAYRSVGEWL EAIKMGRYTE 970 980 990 1000 1010 IFMENGYSSM DSVAQVTLED LRRLGVTLVG HQKKIMNSLQ EMKVQLVNGM VPL
	Isoform 1 [UniParc]. Checksum: BA52CF37A5DAFFAD	FASTA	991	109,937
	10 20 30 40 50 60 MGLRGGGGRA GGPAPGWTCL LLCAALRSLL ASPGSEVNLL DSRTVMGDLG WIAYPKNGWE 70 80 90 100 110 120 EIGEVDENYA PIHTYQVCKV MEQNQNNWLL TSWISNEGRP ASSFELKFTL RDCNSLPGGL 130 140 150 160 170 180 GTCKETFNMY YFESDDEDGR NIRENQYIKI DTIAADESFT ELDLGDRVMK LNTEVRDVGP 190 200 210 220 230 240 LTKKGFYLAF QDVGACIALV SVRVYYKKCP SVIRNLARFP DTITGADSSQ LLEVSGVCVN 250 260 270 280 290 300 HSVTDEAPKM HCSAEGEWLV PIGKCLCKAG YEEKNNTCQV CRPGFFKASP HSPSCSKCPP 310 320 330 340 350 360 HSYTLDEAST SCLCEEHYFR RESDPPTMAC TRPPSAPRSA ISNVNETSVF LEWIPPADTG 370 380 390 400 410 420 GRKDVSYYIA CKKCNSHSGL CEACGSHVRY LPQQTGLKNT SVMMVDLLAH TNYTFEIEAV 430 440 450 460 470 480 NGVSDQNPGA RQFVSVNVTT NQAAPSPVSS VKKGKITKNS ISLSWQEPDR PNGIILEYEI 490 500 510 520 530 540 KYFEKDQETS YTIIKSKETA ITADGLKPGS AYVFQIRART AAGYGGFSRR FEFETSPVLA 550 560 570 580 590 600 ASSDQSQIPI IVVSVTVGVI LLAVVIGFLL SGRRCGYSKA KQDPEEEKMH FHNGHIKLPG 610 620 630 640 650 660 VRTYIDPHTY EDPNQAVHEF AKEIEASCIT IERVIGAGEF GEVCSGRLKL QGKREFPVAI 670 680 690 700 710 720 KTLKVGYTEK QRRDFLGEAS IMGQFDHPNI IHLEGVVTKS KPVMIVTEYM ENGSLDTFLK 730 740 750 760 770 780 KNDGQFTVIQ LVGMLRGIAS GMKYLSDMGY VHRDLAARNI LINSNLVCKV SDFGLSRVLE 790 800 810 820 830 840 DDPEAAYTTR GGKIPIRWTA PEAIAFRKFT SASDVWSYGI VMWEVMSYGE RPYWEMTNQD 850 860 870 880 890 900 VIKAVEEGYR LPSPMDCPAA LYQLMLDCWQ KDRNSRPKFD EIVSMLDKLI RNPSSLKTLV 910 920 930 940 950 960 NASSRVSNLL VEHSPVGSGA YRSVGEWLEA IKMGRYTEIF MENGYSSMDS VAQVTLEDLR 970 980 990 RLGVTLVGHQ KKIMNSLQEM KVQLVNGMVP L
	Isoform 2 [UniParc]. Checksum: 125397E45C0D3A14	FASTA	827	92,018
	10 20 30 40 50 60 MGLRGGGGRA GGPAPGWTCL LLCAALRSLL ASPGSEVNLL DSRTVMGDLG WIAYPKNGWE 70 80 90 100 110 120 EIGEVDENYA PIHTYQVCKV MEQNQNNWLL TSWISNEGRP ASSFELKFTL RDCNSLPGGL 130 140 150 160 170 180 GTCKETFNMY YFESDDEDGR NIRENQYIKI DTIAADESFT ELDLGDRVMK LNTEVRDVGP 190 200 210 220 230 240 LTKKGFYLAF QDVGACIALV SVRVYYKKCP SVIRNLARFP DTITGADSSQ LLEVSGVCVN 250 260 270 280 290 300 HSVTDEAPKM HCSAEGEWLV PIGKCLCKAG YEEKNNTCQA PSPVSSVKKG KITKNSISLS 310 320 330 340 350 360 WQEPDRPNGI ILEYEIKYFE KDQETSYTII KSKETAITAD GLKPGSAYVF QIRARTAAGY 370 380 390 400 410 420 GGFSRRFEFE TSPVLAASSD QSQIPIIVVS VTVGVILLAV VIGFLLSGRR CGYSKAKQDP 430 440 450 460 470 480 EEEKMHFHNG HIKLPGVRTY IDPHTYEDPN QAVHEFAKEI EASCITIERV IGAGEFGEVC 490 500 510 520 530 540 SGRLKLQGKR EFPVAIKTLK VGYTEKQRRD FLGEASIMGQ FDHPNIIHLE GVVTKSKPVM 550 560 570 580 590 600 IVTEYMENGS LDTFLKKNDG QFTVIQLVGM LRGIASGMKY LSDMGYVHRD LAARNILINS 610 620 630 640 650 660 NLVCKVSDFG LSRVLEDDPE AAYTTRGGKI PIRWTAPEAI AFRKFTSASD VWSYGIVMWE 670 680 690 700 710 720 VMSYGERPYW EMTNQDVIKA VEEGYRLPSP MDCPAALYQL MLDCWQKDRN SRPKFDEIVS 730 740 750 760 770 780 MLDKLIRNPS SLKTLVNASS RVSNLLVEHS PVGSGAYRSV GEWLEAIKMG RYTEIFMENG 790 800 810 820 YSSMDSVAQV TLEDLRRLGV TLVGHQKKIM NSLQEMKVQL VNGMVPL

References

[1]	"Identification of a complete Cek7 receptor protein tyrosine kinase coding sequence and cDNAs of alternatively spliced transcripts." Siever D.A., Verderame M.F. Gene 148:219-226(1994) [PubMed: 7958948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Body wall.
[2]	"Five novel avian Eph-related tyrosine kinases are differentially expressed." Sajjadi F.G., Pasquale E.B. Oncogene 8:1807-1813(1993) [PubMed: 8510926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 512-1013. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U03910 mRNA. Translation: AAB60613.1. U03910 mRNA. Translation: AAB60614.1. U03910 mRNA. Translation: AAB60612.1. Z19058 mRNA. Translation: CAA79508.1.
IPI	IPI00583134. IPI00586583. IPI00594604.
PIR	I50615.
UniGene	Gga.3047.
3D structure databases
ProteinModelPortal	P54755.
SMR	P54755. Positions 35-211, 625-936, 942-1002.
ModBase	Search...
Proteomic databases
PRIDE	P54755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	veNOG05547.
GeneTree	ENSGT00600000084371.
HOGENOM	HBG755340.
HOVERGEN	HBG062180.
InParanoid	P54755.
OrthoDB	EOG4894KT.
Enzyme and pathway databases
BRENDA	2.7.10.1. 1306.
Family and domain databases
InterPro	IPR001090. Ephrin_rcpt_lig-bd. IPR003961. Fibronectin_type3. IPR008979. Galactose-bd-like. IPR009030. Growth_fac_rcpt. IPR013783. Ig-like_fold. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. IPR001245. Ser-Thr/Tyr_kinase. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. IPR016257. Tyr_kinase_ephrin_rcpt. IPR001426. Tyr_kinase_rcpt_V_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF07714. Pkinase_Tyr. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00109. TYRKINASE.
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 2 hits. SSF49785. Gal_bind_like. 1 hit. SSF57184. Grow_fac_recept. 1 hit. SSF56112. Kinase_like. 1 hit. SSF47769. SAM_homology. 1 hit.
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS51550. EPH_LBD. 1 hit. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPHA5_CHICK

Accession

Primary (citable) accession number: P54755
Secondary accession number(s): Q07495

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program