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Protein

Ephrin type-A receptor 5

Gene

EPHA5

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei683ATPPROSITE-ProRule annotation1
Active sitei776Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi657 – 665ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Neurogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1306.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-A receptor 5 (EC:2.7.10.1)
Alternative name(s):
EPH-like kinase 7
Short name:
EK7
Short name:
cEK7
Gene namesi
Name:EPHA5
Synonyms:CEK7
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
  • Cell projectionaxon By similarity
  • Cell projectiondendrite By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 549ExtracellularSequence analysisAdd BLAST518
Transmembranei550 – 570HelicalSequence analysisAdd BLAST21
Topological domaini571 – 1013CytoplasmicSequence analysisAdd BLAST443

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000001681532 – 1013Ephrin type-A receptor 5Add BLAST982

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1
Glycosylationi275N-linked (GlcNAc...)Sequence analysis1
Glycosylationi345N-linked (GlcNAc...)Sequence analysis1
Glycosylationi399N-linked (GlcNAc...)Sequence analysis1
Glycosylationi412N-linked (GlcNAc...)Sequence analysis1
Glycosylationi437N-linked (GlcNAc...)Sequence analysis1
Modified residuei626Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei632Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei809Phosphotyrosine; by autocatalysisSequence analysis1
Modified residuei958Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5 (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP54755.
PRIDEiP54755.

Expressioni

Tissue specificityi

Detected in the 10-day embryonic brain, weaker expression in the rest of the 10-day embryo. Undetected in adult tissues.

Interactioni

Subunit structurei

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000019068.

Structurei

3D structure databases

ProteinModelPortaliP54755.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 214Eph LBDPROSITE-ProRule annotationAdd BLAST179
Domaini333 – 443Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST111
Domaini444 – 538Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST95
Domaini651 – 912Protein kinasePROSITE-ProRule annotationAdd BLAST262
Domaini941 – 1013SAMPROSITE-ProRule annotationAdd BLAST73

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1011 – 1013PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi196 – 330Cys-richAdd BLAST135

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
Contains 1 Eph LBD (Eph ligand-binding) domain.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0196. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233856.
HOVERGENiHBG062180.
InParanoidiP54755.
PhylomeDBiP54755.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS01186. EGF_2. 1 hit.
PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 3 (identifier: P54755-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLRGGGGRA GGPAPGWTCL LLCAALRSLL ASPGSEVNLL DSRTVMGDLG
60 70 80 90 100
WIAYPKNGWE EIGEVDENYA PIHTYQVCKV MEQNQNNWLL TSWISNEGRP
110 120 130 140 150
ASSFELKFTL RDCNSLPGGL GTCKETFNMY YFESDDEDGR NIRENQYIKI
160 170 180 190 200
DTIAADESFT ELDLGDRVMK LNTEVRDVGP LTKKGFYLAF QDVGACIALV
210 220 230 240 250
SVRVYYKKCP SVIRNLARFP DTITGADSSQ LLEVSGVCVN HSVTDEAPKM
260 270 280 290 300
HCSAEGEWLV PIGKCLCKAG YEEKNNTCQV CRPGFFKASP HSPSCSKCPP
310 320 330 340 350
HSYTLDEAST SCLCEEHYFR RESDPPTMAC TRPPSAPRSA ISNVNETSVF
360 370 380 390 400
LEWIPPADTG GRKDVSYYIA CKKCNSHSGL CEACGSHVRY LPQQTGLKNT
410 420 430 440 450
SVMMVDLLAH TNYTFEIEAV NGVSDQNPGA RQFVSVNVTT NQAAPSPVSS
460 470 480 490 500
VKKGKITKNS ISLSWQEPDR PNGIILEYEI KYFEKDQETS YTIIKSKETA
510 520 530 540 550
ITADGLKPGS AYVFQIRART AAGYGGFSRR FEFETSPVLA ASSDQSQIPI
560 570 580 590 600
IVVSVTVGVI LLAVVIGFLL SGSCCDHGCG WASSLRAVAY PSLIWRCGYS
610 620 630 640 650
KAKQDPEEEK MHFHNGHIKL PGVRTYIDPH TYEDPNQAVH EFAKEIEASC
660 670 680 690 700
ITIERVIGAG EFGEVCSGRL KLQGKREFPV AIKTLKVGYT EKQRRDFLGE
710 720 730 740 750
ASIMGQFDHP NIIHLEGVVT KSKPVMIVTE YMENGSLDTF LKKNDGQFTV
760 770 780 790 800
IQLVGMLRGI ASGMKYLSDM GYVHRDLAAR NILINSNLVC KVSDFGLSRV
810 820 830 840 850
LEDDPEAAYT TRGGKIPIRW TAPEAIAFRK FTSASDVWSY GIVMWEVMSY
860 870 880 890 900
GERPYWEMTN QDVIKAVEEG YRLPSPMDCP AALYQLMLDC WQKDRNSRPK
910 920 930 940 950
FDEIVSMLDK LIRNPSSLKT LVNASSRVSN LLVEHSPVGS GAYRSVGEWL
960 970 980 990 1000
EAIKMGRYTE IFMENGYSSM DSVAQVTLED LRRLGVTLVG HQKKIMNSLQ
1010
EMKVQLVNGM VPL
Length:1,013
Mass (Da):112,246
Last modified:October 1, 1996 - v1
Checksum:iAC36FD6AEBF38382
GO
Isoform 1 (identifier: P54755-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     573-595: SCCDHGCGWASSLRAVAYPSLIW → R

Show »
Length:991
Mass (Da):109,937
Checksum:iBA52CF37A5DAFFAD
GO
Isoform 2 (identifier: P54755-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     280-443: Missing.
     573-595: SCCDHGCGWASSLRAVAYPSLIW → R

Show »
Length:827
Mass (Da):92,018
Checksum:i125397E45C0D3A14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti981 – 1013LRRLG…GMVPL → ESPCEKWSLTLHPLFPTGYQ T in CAA79508 (PubMed:8510926).CuratedAdd BLAST33

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003004280 – 443Missing in isoform 2. CuratedAdd BLAST164
Alternative sequenceiVSP_003005573 – 595SCCDH…PSLIW → R in isoform 1 and isoform 2. CuratedAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03910 mRNA. Translation: AAB60613.1.
U03910 mRNA. Translation: AAB60614.1.
U03910 mRNA. Translation: AAB60612.1.
Z19058 mRNA. Translation: CAA79508.1.
PIRiI50615.
UniGeneiGga.3047.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03910 mRNA. Translation: AAB60613.1.
U03910 mRNA. Translation: AAB60614.1.
U03910 mRNA. Translation: AAB60612.1.
Z19058 mRNA. Translation: CAA79508.1.
PIRiI50615.
UniGeneiGga.3047.

3D structure databases

ProteinModelPortaliP54755.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000019068.

Proteomic databases

PaxDbiP54755.
PRIDEiP54755.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0196. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233856.
HOVERGENiHBG062180.
InParanoidiP54755.
PhylomeDBiP54755.

Enzyme and pathway databases

BRENDAi2.7.10.1. 1306.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS01186. EGF_2. 1 hit.
PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPHA5_CHICK
AccessioniPrimary (citable) accession number: P54755
Secondary accession number(s): Q07495
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.