Ephrin type-B receptor 3 precursor - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot P54754 (EPHB3_MOUSE)

Last modified February 9, 2010. Version 103. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Ephrin type-B receptor 3
    EC=2.7.10.1
Alternative name(s):
    Developmental kinase 5
      Short name=mDK-5
    Tyrosine-protein kinase receptor SEK-4

Gene names

Name:	Ephb3
Synonyms:	Etk2, Mdk5, Sek4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	993 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Receptor for members of the ephrin-B family.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

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Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	central nervous system projection neuron axonogenesis Inferred from direct assay. Source: MGI protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro retinal ganglion cell axon guidance Inferred from direct assay. Source: MGI transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to plasma membrane Traceable author statement. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW axon guidance receptor activity Inferred from direct assay. Source: MGI protein binding Inferred from electronic annotation. Source: InterPro transmembrane-ephrin receptor activity Traceable author statement. Source: MGI
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 29

Potential

Chain

30 – 993

964

Ephrin type-B receptor 3

PRO_0000016832

Regions

Topological domain

30 – 554

525

Extracellular Potential

Transmembrane

555 – 575

Potential

Topological domain

576 – 993

418

Cytoplasmic Potential

Domain

332 – 438

107

Fibronectin type-III 1

Domain

448 – 539

Fibronectin type-III 2

Domain

628 – 891

264

Protein kinase

Domain

920 – 984

SAM

Nucleotide binding

634 – 642

ATP By similarity

Motif

991 – 993

PDZ-binding Potential

Compositional bias

191 – 328

138

Cys-rich

Sites

Active site

753

Proton acceptor By similarity

Binding site

660

ATP By similarity

Amino acid modifications

Modified residue

595

Phosphotyrosine By similarity

Modified residue

603

Phosphotyrosine By similarity

Modified residue

609

Phosphotyrosine By similarity

Modified residue

787

Phosphotyrosine By similarity

Modified residue

966

Phosphothreonine By similarity

Glycosylation

343

N-linked (GlcNAc...) Potential

Glycosylation

440

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

719

R → Q in CAA53599. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P54754-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 0B66A4D391266C79
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FASTA

993

109,586

        10         20         30         40         50         60 
MAGARPPPGL LPLLAPLLLP LLLPAGCWAL EETLMDTKWV TSELAWTSHP ESGWEEVSGY 

        70         80         90        100        110        120 
DEAMNPIRTY QVCNVRESSQ NNWLRTGFIW RREVQRVYVE LKFTVRDCNS IPNIPGSCKE 

       130        140        150        160        170        180 
TFNLFYYEAD SDVASASSPF WMENPYVKVD TIAPDESFSR LDAGRVNTKV RSFGPLSKAG 

       190        200        210        220        230        240 
FYLAFQDQGA CMSLISVRAG DKKCASTTAG FALFPETLTG AEPTSLVIAP GTCIANAVEV 

       250        260        270        280        290        300 
SVPLKLYCNG DGEWMVPVGA CTCATGHEPA AKESQCRACP PGSYKAKQGE GPCLPCPPNS 

       310        320        330        340        350        360 
RTTSPAASIC TCHNNFYRAD SDSADSACTT EPSPPRGVIS NVNETSLILE WSEPRDLGGR 

       370        380        390        400        410        420 
DDLLYNVICK KCRGSSGAGG PATCSRCDDN VEFVPRQLGL TERRVHISHL LAHTRYTFEV 

       430        440        450        460        470        480 
QAVNGVSGKS PLPPRYAAVN ITTNQAAPSE VPTLHLHSSS GSSLTLSWAP PERPNGVILD 

       490        500        510        520        530        540 
YEMKYFEKSK GIASTVTSQK NSVQLDGLQP DARYVVQVRA RTVAGYGQYS HPAEFETTSE 

       550        560        570        580        590        600 
RGSGAQQLQE QLPLIVGSTV AGFVFMMVVV VIALVCLRKQ RHGPDAEYTE KLQQYIAPGM 

       610        620        630        640        650        660 
KVYIDPFTYE DPNEAVREFA KEIDVSCVKI EEVIGAGEFG EVCRGRLKLP GRREVFVAIK 

       670        680        690        700        710        720 
TLKVGYTERQ RRDFLSEASI MGQFDHPNII RLEGVVTKSR PVMILTEFME NCALDSFLRL 

       730        740        750        760        770        780 
NDGQFTVIQL VGMLRGIAAG MKYLSEMNYV HRDLAARNIL VNSNLVCKVS DFGLSRFLED 

       790        800        810        820        830        840 
DPSDPTYTSS LGGKIPIRWT APEAIAYRKF TSASDVWSYG IVMWEVMSYG ERPYWDMSNQ 

       850        860        870        880        890        900 
DVINAVEQDY RLPPPMDCPT ALHQLMLDCW VRDRNLRPKF SQIVNTLDKL IRNAASLKVT 

       910        920        930        940        950        960 
ASAPSGMSQP LLDRTVPDYT TFTTVGDWLD AIKMGRYKES FVGAGFASFD LVAQMTAEDL 

       970        980        990 
LRIGVTLAGH QKKILCSIQD MRLQMNQTLP VQV

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References

[1]	"Cloning, characterization, and differential expression of MDK2 and MDK5, two novel receptor tyrosine kinases of the eck/eph family." Ciossek T., Lerch M.M., Ullrich A. Oncogene 11:2085-2095(1995) [PubMed: 7478528] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Embryo.
[2]	"Several receptor tyrosine kinase genes of the Eph family are segmentally expressed in the developing hindbrain." Becker N., Seitanidou T., Murphy P., Mattei M.-G., Topilko P., Nieto A., Wilkinson D.G., Charnay P., Gilardi P. Mech. Dev. 47:3-17(1994) [PubMed: 7947319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 719-993. Strain: BALB/c. Tissue: Brain.
[3]	"Complementary expression of transmembrane ephrins and their receptors in the mouse spinal cord: a possible role in constraining the orientation of longitudinally projecting axons." Imondi R., Wideman C., Kaprielian Z. Development 127:1397-1410(2000) [PubMed: 10704386] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49086 mRNA. Translation: CAA88910.1. X76012 mRNA. Translation: CAA53599.1.
IPI	IPI00130442.
PIR	I48653. I48761.
UniGene	Mm.6972
3D structure databases
SMR	P54754. Positions 30-204, 445-542, 601-898, 909-988.
ModBase	Search...
Protein-protein interaction databases
STRING	P54754.
PTM databases
PhosphoSite	P54754.
Proteomic databases
PRIDE	P54754.
Genome annotation databases
Ensembl	ENSMUST00000006112; ENSMUSP00000006112; ENSMUSG00000005958; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:104770. Ephb3.
Phylogenomic databases
eggNOG	roNOG12692.
HOGENOM	HBG755340.
HOVERGEN	P54754.
InParanoid	P54754.
Enzyme and pathway databases
BRENDA	2.7.10.1. 244.
Gene expression databases
ArrayExpress	P54754.
Bgee	P54754.
CleanEx	MM_EPHB3.
Genevestigator	P54754.
GermOnline	ENSMUSG00000005958. Mus musculus.
Family and domain databases
InterPro	IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR008979. Galactose-bd-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR010993. SAM_homology. IPR013761. SAM_type. IPR021129. SAM_type1. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR016257. Tyr_prot_kinase_ephrin_rcpt. IPR001426. Tyr_prot_kinase_rcpt_V_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF00536. SAM_1. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII.
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

EPHB3_MOUSE

Accession

Primary (citable) accession number: P54754
Secondary accession number(s): Q62214

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents