Reviewed, UniProtKB/Swiss-Prot P54752 (ARGD_ANASP)

Names and origin

Protein names

Recommended name:
    Acetylornithine aminotransferase
      Short name=ACOAT
    EC=2.6.1.11

Gene names

Name:	argD
Ordered Locus Names:	alr1080

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate. HAMAP MF_01107
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4. HAMAP MF_01107
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Miscellaneous	May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis. HAMAP MF_01107
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Acetylornithine aminotransferase HAMAP MF_01107		PRO_0000112714
Regions
	Region	248 – 251	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	157	1	Pyridoxal phosphate; via carbonyl oxygen By similarity
	Binding site	160	1	N(2)-acetyl-L-ornithine By similarity
	Binding site	304	1	N(2)-acetyl-L-ornithine By similarity
	Binding site	305	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	277	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54752-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6AF594BB4B3B134E	FASTA	427	46,140
	10 20 30 40 50 60 MSLQTLIEQA TNPPESGSAA SSPFSTDSFD ASVMSTYGRF PLALERGAGC RVWDTQGKEY 70 80 90 100 110 120 LDFVAGIATC TLGHAHPAMV EAVTRQIQKL HHVSNLYYIP EQGELAQWII QHSCADRVFF 130 140 150 160 170 180 CNSGAEANEA AIKLARKYAH TVLDIEKPII LTANASFHGR TLATITATGQ AKYQKYFDPL 190 200 210 220 230 240 VPGFHYVNYN DISAVEAAIS ELDEGDYRVA AILIEPLQGE GGVRPGDVEY FQKLRQICDD 250 260 270 280 290 300 TGILLMFDEV QVGMGRSGKL WGYEYLGVEP DIFTSAKGLG GGIPIGAMMS KKFCDVFQPG 310 320 330 340 350 360 EHASTFGGNP FACGVALAVC QTLERENILQ NVQDRGEQLR SGLRAIAAKY PHHLTEVRGW 370 380 390 400 410 420 GLINGLELAA DIPLTAADVV KAAINEGLLL VPAGPKVVRF VPPLIVTEAE INTALKLLEK ALATVTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of expression of the argC and argD genes in the cyanobacterium Anabaena sp. strain PCC 7120." Floriano B., Herrero A., Flores E. J. Bacteriol. 176:6397-6401(1994) [PubMed: 7929012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78854 Genomic DNA. Translation: CAA55410.1. BA000019 Genomic DNA. Translation: BAB73037.1.
PIR	AE1941.
RefSeq	NP_485123.1.
3D structure databases
HSSP	HSSP built from PDB template 1QJ3 based on UniProtKB P12995.
ModBase	Search...
Genome annotation databases
GeneID	1104674.
GenomeReviews	Gene locus alr1080 in contig BA000019_GR.
KEGG	ana:alr1080.
NMPDR	fig|103690.1.peg.1390.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P54752.
OMA	P54752. EHNALLV.
Enzyme and pathway databases
BioCyc	NSP103690:ALR1080-MON.
Family and domain databases
HAMAP	MF_01107. [Tree]
InterPro	IPR005814. Aminotrans_3. IPR004636. ArgD_aminotrans. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF19. ArgD_aminotrans. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00707. argD. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGD_ANASP

Accession

Primary (citable) accession number: P54752

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)