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Protein

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1

Gene

St3gal1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of the sequence NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- found on sugar chains O-linked to Thr or Ser and also as a terminal sequence on certain gangliosides. SIAT4A and SIAT4B sialylate the same acceptor substrates but exhibit different Km values.2 Publications

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-R.2 Publications

Kineticsi

Vmax is 4 fold higher with Gal-beta-1,3-GalNAc than with Gal-beta-1,3-GlcNAc as substrate.1 Publication
  1. KM=3.2 mM for Gal-beta-1,3-GlcNAc1 Publication
  2. KM=51 µM for Gal-beta-1,3-GalNAc1 Publication
  3. KM=0.11 mM for Gal-beta-1,3-GalNAc1 Publication
  4. KM=0.03 mM for CMP-N-acetyl-beta-neuraminate1 Publication
  5. KM=1.5 mM for GM11 Publication
  6. KM=0.37 mM for asialo-GM11 Publication

    pH dependencei

    Optimum pH is 6.4.1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei102SubstrateBy similarity1
    Binding sitei144SubstrateBy similarity1
    Binding sitei167SubstrateBy similarity1
    Binding sitei227SubstrateBy similarity1
    Binding sitei263SubstrateBy similarity1
    Binding sitei267Substrate; via amide nitrogenBy similarity1
    Binding sitei287Substrate; via amide nitrogenBy similarity1
    Binding sitei296SubstrateBy similarity1
    Binding sitei313SubstrateBy similarity1

    GO - Molecular functioni

    • beta-galactoside (CMP) alpha-2,3-sialyltransferase activity Source: MGI

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.99.4 3474
    ReactomeiR-MMU-2022854 Keratan sulfate biosynthesis
    R-MMU-4085001 Sialic acid metabolism
    R-MMU-977068 Termination of O-glycan biosynthesis
    UniPathwayiUPA00378

    Protein family/group databases

    CAZyiGT29 Glycosyltransferase Family 29

    Chemistry databases

    SwissLipidsiSLP:000001413

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 (EC:2.4.99.42 Publications)
    Short name:
    Alpha 2,3-ST 1
    Short name:
    Beta-galactoside alpha-2,3-sialyltransferase 1
    Alternative name(s):
    Gal-NAc6S
    Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
    ST3Gal I
    Short name:
    ST3GalI
    ST3GalA.1
    ST3O
    Sialyltransferase 4A
    Short name:
    SIAT4-A
    Gene namesi
    Name:St3gal1
    Synonyms:Siat4, Siat4a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 15

    Organism-specific databases

    MGIiMGI:98304 St3gal1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 4CytoplasmicSequence analysis4
    Transmembranei5 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini26 – 337LumenalSequence analysisAdd BLAST312

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001492541 – 337CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1Add BLAST337

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi56 ↔ 61By similarity
    Disulfide bondi58 ↔ 136By similarity
    Glycosylationi76N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi139 ↔ 278By similarity
    Glycosylationi320N-linked (GlcNAc...) asparagineSequence analysis1

    Post-translational modificationi

    The soluble form derives from the membrane form by proteolytic processing.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    EPDiP54751
    PaxDbiP54751
    PeptideAtlasiP54751
    PRIDEiP54751

    PTM databases

    iPTMnetiP54751
    PhosphoSitePlusiP54751

    Expressioni

    Tissue specificityi

    Highly expressed in submaxillary gland and to a much lesser extent in liver, lung, kidney, heart and brain.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000013846
    ExpressionAtlasiP54751 baseline and differential
    GenevisibleiP54751 MM

    Interactioni

    Protein-protein interaction databases

    IntActiP54751, 1 interactor
    MINTiP54751
    STRINGi10090.ENSMUSP00000090307

    Structurei

    3D structure databases

    ProteinModelPortaliP54751
    SMRiP54751
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2692 Eukaryota
    ENOG410XT8P LUCA
    GeneTreeiENSGT00760000119095
    HOGENOMiHOG000126811
    HOVERGENiHBG054227
    InParanoidiP54751
    KOiK00780
    OMAiTWFPKQM
    OrthoDBiEOG091G08DE
    PhylomeDBiP54751
    TreeFamiTF354325

    Family and domain databases

    Gene3Di3.90.1480.20, 1 hit
    InterProiView protein in InterPro
    IPR001675 Glyco_trans_29
    IPR038578 GT29-like_sf
    IPR012163 Sialyl_trans
    PfamiView protein in Pfam
    PF00777 Glyco_transf_29, 1 hit
    PIRSFiPIRSF005557 Sialyl_trans, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P54751-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRRKTLKYLT FFLLFIFLTS FVLNYSNTGV PSAWFPKQML LELSENFRRF
    60 70 80 90 100
    IKSQPCTCRH CISQDKVSYW FDQRFNKTMQ PLLTVHNALM EEDTYRWWLR
    110 120 130 140 150
    LQRERKPNNL SDTVKELFRL VPGNVDPMLN KRLVGCRRCA VVGNSGNLKD
    160 170 180 190 200
    SSYGPEIDSH DFVLRMNKAP TVGFEADVGS RTTHHLVYPE SFRELGENVN
    210 220 230 240 250
    MVLVPFKTTD LQWVISATTT GTITHTYVPV PPKIKVKQEK ILIYHPAFIK
    260 270 280 290 300
    YVFDNWLQGH GRYPSTGILS IIFSIHICDE VDLYGFGADS KGNWHHYWEN
    310 320 330
    NPSAGAFRKT GVHDGDFEYN ITTTLAAINK IRIFKGR
    Length:337
    Mass (Da):39,073
    Last modified:October 1, 1996 - v1
    Checksum:iCB54929D1EC047D1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X73523 mRNA Translation: CAA51919.1
    BC084730 mRNA Translation: AAH84730.1
    CCDSiCCDS27511.1
    PIRiS36824
    RefSeqiNP_033203.1, NM_009177.4
    XP_006520727.1, XM_006520664.3
    XP_006520728.1, XM_006520665.3
    XP_011243831.1, XM_011245529.2
    UniGeneiMm.248334
    Mm.442417
    Mm.461082

    Genome annotation databases

    EnsembliENSMUST00000092640; ENSMUSP00000090307; ENSMUSG00000013846
    GeneIDi20442
    KEGGimmu:20442
    UCSCiuc007wba.1 mouse

    Similar proteinsi

    Entry informationi

    Entry nameiSIA4A_MOUSE
    AccessioniPrimary (citable) accession number: P54751
    Secondary accession number(s): Q11202
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: March 28, 2018
    This is version 142 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

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