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Protein

Mannosylglycerate hydrolase

Gene

mngB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May hydrolyze mannosyl-D-glycerate to mannose-6-phosphate and glycerate.1 Publication

Catalytic activityi

2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate + H2O = D-mannose 6-phosphate + D-glycerate.1 Publication

Cofactori

a divalent metal cationBy similarityNote: Binds 1 divalent metal cation per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14Divalent metal cationBy similarity1
Metal bindingi16Divalent metal cationBy similarity1
Active sitei126NucleophileBy similarity1
Metal bindingi126Divalent metal cationBy similarity1
Metal bindingi338Divalent metal cationBy similarity1

GO - Molecular functioni

  • alpha-mannosidase activity Source: EcoliWiki
  • carbohydrate binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG13236-MONOMER.
ECOL316407:JW0721-MONOMER.
MetaCyc:EG13236-MONOMER.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosylglycerate hydrolase (EC:3.2.1.170)
Alternative name(s):
2-O-(6-phospho-mannosyl)-D-glycerate hydrolase
Alpha-mannosidase mngB
Gene namesi
Name:mngB
Synonyms:ybgG
Ordered Locus Names:b0732, JW0721
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13236. mngB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001687001 – 877Mannosylglycerate hydrolaseAdd BLAST877

Proteomic databases

PaxDbiP54746.
PRIDEiP54746.

Expressioni

Inductioni

Repressed by MngR. Induced by mannosyl-D-glycerate.

Interactioni

Protein-protein interaction databases

BioGridi4263542. 12 interactors.
IntActiP54746. 14 interactors.
STRINGi511145.b0732.

Structurei

3D structure databases

ProteinModelPortaliP54746.
SMRiP54746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Phylogenomic databases

eggNOGiENOG4105C8I. Bacteria.
COG0383. LUCA.
HOGENOMiHOG000231434.
InParanoidiP54746.
KOiK15524.
OMAiLENTDRY.
PhylomeDBiP54746.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

P54746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVSRVHIT PHMHWDREWY FTTEESRILL VNNMEEILCR LEQDNEYKYY
60 70 80 90 100
VLDGQTAILE DYFAVKPENK DRVKKQVEAG KLIIGPWYTQ TDTTIVSAES
110 120 130 140 150
IVRNLMYGMR DCLAFGEPMK IGYLPDSFGM SGQLPHIYNG FGITRTMFWR
160 170 180 190 200
GCSERHGTDK TEFLWQSSDG SEVTAQVLPL GYAIGKYLPA DENGLRKRLD
210 220 230 240 250
SYFDVLEKAS VTKEILLPNG HDQMPLQQNI FEVMDKLREI YPQRKFVMSR
260 270 280 290 300
FEEVFEKIEA QRDNLATLKG EFIDGKYMRV HRTIGSTRMD IKIAHARIEN
310 320 330 340 350
KIVNLLEPLA TLAWTLGFEY HHGLLEKMWK EILKNHAHDS IGCCCSDKVH
360 370 380 390 400
REIVARFELA EDMADNLIRF YMRKIADNMP QSDADKLVLF NLMPWPREEV
410 420 430 440 450
INTTVRLRAS QFNLRDDRGQ PVPYFIRHAR EIDPGLIDRQ IVHYGNYDPF
460 470 480 490 500
MEFDIQINQI VPSMGYRTLY IEANQPGNVI AAKSDAEGIL ENAFWQIALN
510 520 530 540 550
EDGSLQLVDK DSGVRYDRVL QIEESSDDGD EYDYSPAKEE WVITAANAKP
560 570 580 590 600
QCDIIHEAWQ SRAVIRYDMA VPLNLSERSA RQSTGRVGVV LVVTLSHNSR
610 620 630 640 650
RIDVDINLDN QADDHRLRVL VPTPFNTDSV LADTQFGSLT RPVNDSAMNN
660 670 680 690 700
WQQEGWKEAP VPVWNMLNYV ALQEGRNGMA VFSEGLREFE VIGEEKKTFA
710 720 730 740 750
ITLLRGVGLL GKEDLLLRPG RPSGIKMPVP DSQLRGLLSC RLSLLSYTGT
760 770 780 790 800
PTAAGVAQQA RAWLTPVQCY NKIPWDVMKL NKAGFNVPES YSLLKMPPVG
810 820 830 840 850
CLISALKKAE DRQEVILRLF NPAESATCDA TVAFSREVIS CSETMMDEHI
860 870
TTEENQGSNL SGPFLPGQSR TFSYRLA
Length:877
Mass (Da):100,015
Last modified:November 1, 1997 - v2
Checksum:iB67201C6F8CB1C0A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87 – 100WYTQT…VSAES → GIPRPIPRLFCGNP (PubMed:9063979).CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73826.1.
AP009048 Genomic DNA. Translation: BAA35398.1.
D64014 Genomic DNA. No translation available.
PIRiC64809.
RefSeqiNP_415260.1. NC_000913.3.
WP_000648976.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73826; AAC73826; b0732.
BAA35398; BAA35398; BAA35398.
GeneIDi945359.
KEGGiecj:JW0721.
eco:b0732.
PATRICi32116659. VBIEscCol129921_0762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73826.1.
AP009048 Genomic DNA. Translation: BAA35398.1.
D64014 Genomic DNA. No translation available.
PIRiC64809.
RefSeqiNP_415260.1. NC_000913.3.
WP_000648976.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP54746.
SMRiP54746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263542. 12 interactors.
IntActiP54746. 14 interactors.
STRINGi511145.b0732.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Proteomic databases

PaxDbiP54746.
PRIDEiP54746.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73826; AAC73826; b0732.
BAA35398; BAA35398; BAA35398.
GeneIDi945359.
KEGGiecj:JW0721.
eco:b0732.
PATRICi32116659. VBIEscCol129921_0762.

Organism-specific databases

EchoBASEiEB3025.
EcoGeneiEG13236. mngB.

Phylogenomic databases

eggNOGiENOG4105C8I. Bacteria.
COG0383. LUCA.
HOGENOMiHOG000231434.
InParanoidiP54746.
KOiK15524.
OMAiLENTDRY.
PhylomeDBiP54746.

Enzyme and pathway databases

BioCyciEcoCyc:EG13236-MONOMER.
ECOL316407:JW0721-MONOMER.
MetaCyc:EG13236-MONOMER.

Miscellaneous databases

PROiP54746.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNGB_ECOLI
AccessioniPrimary (citable) accession number: P54746
Secondary accession number(s): P75753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.