P54746 (MNGB_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 95.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Mannosylglycerate hydrolase EC=3.2.1.170 Alternative name(s): 2-O-(6-phospho-mannosyl)-D-glycerate hydrolase Alpha-mannosidase mngB | ||||||
| Gene names |
| ||||||
| Organism | Escherichia coli (strain K12) [Reference proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › ![]() |
Protein attributes
| Sequence length | 877 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | May hydrolyze mannosyl-D-glycerate to mannose-6-phosphate and glycerate. Ref.5 |
| Catalytic activity | 2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate + H2O = D-mannose 6-phosphate + D-glycerate. Ref.5 |
| Cofactor | Binds one divalent metal cation per subunit By similarity. |
| Induction | Repressed by MngR. Induced by mannosyl-D-glycerate. |
| Sequence similarities | Belongs to the glycosyl hydrolase 38 family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Metal-binding |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | mannose metabolic process Inferred from electronic annotation. Source: InterPro |
| Molecular_function | alpha-mannosidase activity Inferred from mutant phenotype Ref.5. Source: EcoliWiki carbohydrate bindingInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 877 | 877 | Mannosylglycerate hydrolase | PRO_0000168700 | |||||
Sites | |||||||||
| Active site | 126 | 1 | Nucleophile By similarity | ||||||
| Metal binding | 14 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 16 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 126 | 1 | Divalent metal cation By similarity | ||||||
| Metal binding | 338 | 1 | Divalent metal cation By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 87 – 100 | 14 | WYTQT…VSAES → GIPRPIPRLFCGNP Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T.DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | "Isolation and characterization of the heat-responsive genes in Escherichia coli." Utsumi R., Horie T., Katoh A., Kaino Y., Tanabe H., Noda M. Biosci. Biotechnol. Biochem. 60:309-315(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100. |
| [5] | "Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor." Sampaio M.-M., Chevance F., Dippel R., Eppler T., Schlegel A., Boos W., Lu Y.-J., Rock C.O. J. Biol. Chem. 279:5537-5548(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A MANNOSIDASE, CATALYTIC ACTIVITY. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U00096 Genomic DNA. Translation: AAC73826.1. AP009048 Genomic DNA. Translation: BAA35398.1. D64014 Genomic DNA. No translation available. |
| PIR | C64809. |
| RefSeq | NP_415260.1. NC_000913.2. YP_489011.1. NC_007779.1. |
3D structure databases | |
| ProteinModelPortal | P54746. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P54746. 14 interactions. |
| STRING | 511145.b0732. |
Protein family/group databases | |
| CAZy | GH38. Glycoside Hydrolase Family 38. |
Proteomic databases | |
| PaxDb | P54746. |
| PRIDE | P54746. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAC73826; AAC73826; b0732. BAA35398; BAA35398; BAA35398. |
| GeneID | 12934548. 945359. |
| KEGG | ecj:Y75_p0711. eco:b0732. |
| PATRIC | 32116659. VBIEscCol129921_0762. |
Organism-specific databases | |
| EchoBASE | EB3025. |
| EcoGene | EG13236. mngB. |
Phylogenomic databases | |
| eggNOG | COG0383. |
| HOGENOM | HOG000231434. |
| KO | K15524. |
| OMA | IRYEMAV. |
| ProtClustDB | PRK09819. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:EG13236-MONOMER. ECOL316407:JW0721-MONOMER. MetaCyc:EG13236-MONOMER. |
Gene expression databases | |
| Genevestigator | P54746. |
Family and domain databases | |
| Gene3D | 3.20.110.10. 1 hit. |
| InterPro | IPR011013. Gal_mutarotase_SF_dom. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR027291. Glyco_hydro_38/57_N. IPR011682. Glyco_hydro_38_C. IPR015341. Glyco_hydro_38_cen_dom. IPR000602. Glyco_hydro_38_N. [Graphical view] |
| Pfam | PF09261. Alpha-mann_mid. 1 hit. PF01074. Glyco_hydro_38. 1 hit. PF07748. Glyco_hydro_38C. 1 hit. [Graphical view] |
| SMART | SM00872. Alpha-mann_mid. 1 hit. [Graphical view] |
| SUPFAM | SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | MNGB_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P54746 Secondary accession number(s): P75753 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with
