Heat-responsive suppressor hrsA - Escherichia coli (strain K12)

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P54745 (HRSA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Heat-responsive suppressor hrsA

Alternative name(s):
Putative PTS system EIIABC component

Including the following 3 domains:

Phosphotransferase enzyme IIA component
EC=2.7.1.-
Alternative name(s):
PTS system EIIA component
Phosphotransferase enzyme IIB component
EC=2.7.1.69
Alternative name(s):
PTS system EIIB component
Permease IIC component
Alternative name(s):
PTS system EIIC component

Gene names

Name:	hrsA
Ordered Locus Names:	b0731, JW0720

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in thermoinduction of ompC. The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Domain	The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 658

658

Heat-responsive suppressor hrsA

PRO_0000186699

Regions

Topological domain

1 – 313

313

Periplasmic Potential

Transmembrane

314 – 334

Helical; Potential

Topological domain

335 – 358

Cytoplasmic Potential

Transmembrane

359 – 379

Helical; Potential

Topological domain

380 – 389

Periplasmic Potential

Transmembrane

390 – 410

Helical; Potential

Topological domain

411 – 433

Cytoplasmic Potential

Transmembrane

434 – 454

Helical; Potential

Topological domain

455 – 474

Periplasmic Potential

Transmembrane

475 – 495

Helical; Potential

Topological domain

496 – 500

Cytoplasmic Potential

Transmembrane

501 – 521

Helical; Potential

Topological domain

522 – 551

Periplasmic Potential

Transmembrane

552 – 572

Helical; Potential

Topological domain

573

Cytoplasmic Potential

Transmembrane

574 – 594

Helical; Potential

Topological domain

595 – 620

Periplasmic Potential

Transmembrane

621 – 641

Helical; Potential

Topological domain

642 – 658

Cytoplasmic Potential

Domain

25 – 171

147

PTS EIIA type-2

Domain

186 – 282

PTS EIIB type-2

Domain

306 – 641

336

PTS EIIC type-2

Sites

Active site

Tele-phosphohistidine intermediate; for EIIA activity By similarity

Active site

192

Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P54745 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: C1F0999A1C48C5C2
Show »

FASTA

658

69,668

        10         20         30         40         50         60 
MVLFYRAHWR DYKNDQVRIM MNLTTLTHRD ALCLNARFTS REEAIHALTQ RLAALGKISS 

        70         80         90        100        110        120 
TEQFLEEVYR RESLGPTALG EGLAVPHGKT AAVKEAAFAV ATLSEPLQWE GVDGPEAVDL 

       130        140        150        160        170        180 
VVLLAIPPNE AGTTHMQLLT ALTTRLADDE IRARIQSATT PDELLSALDD KGGTQPSASF 

       190        200        210        220        230        240 
SNAPTIVCVT ACPAGIAHTY MAAEYLEKAG RKLGVNVYVE KQGANGIEGR LTADQLNSAT 

       250        260        270        280        290        300 
ACIFAAEVAI KESERFNGIP ALSVPVAEPI RHAEALIQQA LTLKRSDETR TVQQDTQPVK 

       310        320        330        340        350        360 
SVKTELKQAL LSGISFAVPL IVAGGTVLAV AVLLSQIFGL QDLFNEENSW LWMYRKLGGG 

       370        380        390        400        410        420 
LLGILMVPVL AAYTAYSLAD KPALAPGFAA GLAANMIGSG FLGAVVGGLI AGYLMRWVKN 

       430        440        450        460        470        480 
HLRLSSKFNG FLTFYLYPVL GTLGAGSLML FVVGEPVAWI NNSLTAWLNG LSGSNALLLG 

       490        500        510        520        530        540 
AILGFMCSFD LGGPVNKAAY AFCLGAMANG VYGPYAIFAS VKMVSAFTVT ASTMLAPRLF 

       550        560        570        580        590        600 
KEFEIETGKS TWLLGLAGIT EGAIPMAIED PLRVIGSFVL GSMVTGAIVG AMNIGLSTPG 

       610        620        630        640        650 
AGIFSLFLLH DNGAGGVMAA IGWFGAALVG AAISTAILLM WRRHAVKHGN YLTDGVMP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the heat-responsive genes in Escherichia coli." Utsumi R., Horie T., Katoh A., Kaino Y., Tanabe H., Noda M. Biosci. Biotechnol. Biochem. 60:309-315(1996) [PubMed: 9063979] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 277-658. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D64014 Genomic DNA. Translation: BAA10893.1. U00096 Genomic DNA. Translation: AAC73825.1. AP009048 Genomic DNA. Translation: BAA35397.2.
PIR	JC4598.
RefSeq	NP_415259.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P54745.
SMR	P54745. Positions 27-172, 183-287.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9939N.
IntAct	P54745. 1 interaction.
MINT	MINT-1249398.
Protein family/group databases
TCDB	4.A.2.1.3. PTS fructose-mannitol (Fru) family.
Proteomic databases
PRIDE	P54745.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002928; EBESCP00000002928; EBESCG00000002393. EBESCT00000017038; EBESCP00000016329; EBESCG00000016097.
GeneID	945355.
GenomeReviews	Gene locus JW0720 in contig AP009048_GR. Gene locus b0731 in contig U00096_GR.
KEGG	ecj:JW0720. eco:b0731.
PATRIC	32116657. VBIEscCol129921_0761.
Organism-specific databases
EchoBASE	EB3024.
EcoGene	EG13235. hrsA.
Phylogenomic databases
eggNOG	COG1299.
GeneTree	EBGT00070000031702.
HOGENOM	HBG704192.
OMA	MVSFDLG.
PhylomeDB	P54745.
ProtClustDB	PRK09765.
Enzyme and pathway databases
BioCyc	EcoCyc:HRSA-MONOMER.
Gene expression databases
Genevestigator	P54745.
Family and domain databases
InterPro	IPR016152. PTrfase/Anion_transptr. IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR004715. PTS_IIA_fruc. IPR003353. PTS_IIB_fruc. IPR006327. PTS_IIC_fruc. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
KO	K11198. K11199. K11200.
Pfam	PF00359. PTS_EIIA_2. 1 hit. PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
SUPFAM	SSF55804. PTrfase/Anion_transptr. 1 hit.
TIGRFAMs	TIGR00829. FRU. 1 hit. TIGR00848. FruA. 1 hit. TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HRSA_ECOLI

Accession

Primary (citable) accession number: P54745

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 3 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents