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P54744

- PKNB_MYCLE

UniProt

P54744 - PKNB_MYCLE

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Protein

Serine/threonine-protein kinase PknB

Gene
pknB, ML0016
Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATP By similarity
Active sitei138 – 1381Proton acceptor By similarity
Metal bindingi143 – 1431Magnesium By similarity
Metal bindingi156 – 1561Magnesium By similarity
Binding sitei156 – 1561ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATP By similarity
Nucleotide bindingi93 – 953ATP By similarity
Nucleotide bindingi140 – 1434ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. penicillin binding Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PknB (EC:2.7.11.1)
Gene namesi
Name:pknB
Ordered Locus Names:ML0016
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML0016.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 328328Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei329 – 34921Helical; Reviewed predictionAdd
BLAST
Topological domaini350 – 622273Extracellular Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Serine/threonine-protein kinase PknBPRO_0000171207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei166 – 1661Phosphoserine; by autocatalysis By similarity
Modified residuei168 – 1681Phosphoserine; by autocatalysis By similarity
Modified residuei170 – 1701Phosphothreonine; by autocatalysis By similarity
Modified residuei172 – 1721Phosphothreonine; by autocatalysis By similarity
Modified residuei308 – 3081Phosphothreonine; by autocatalysis By similarity

Post-translational modificationi

Autophosphorylated. Dephosphorylated by PstP By similarity.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi272631.ML0016.

Structurei

3D structure databases

ProteinModelPortaliP54744.
SMRiP54744. Positions 1-285.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 273263Protein kinaseAdd
BLAST
Domaini352 – 41867PASTA 1Add
BLAST
Domaini419 – 48668PASTA 2Add
BLAST
Domaini487 – 55367PASTA 3Add
BLAST
Domaini554 – 62269PASTA 4Add
BLAST

Sequence similaritiesi

Contains 4 PASTA domains.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000037185.
KOiK08884.
OMAiAINFLES.
OrthoDBiEOG6B35XT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54744-1 [UniParc]FASTAAdd to Basket

« Hide

MTTPPHLSDR YELGDILGFG GMSEVHLARD IRLHRDVAVK VLRADLARDP    50
SFYLRFRREA QNAAALNHPS IVAVYDTGEA ETSAGPLPYI VMEYVDGATL 100
RDIVHTDGPM PPQQAIEIVA DACQALNFSH QNGIIHRDVK PANIMISATN 150
AVKVMDFGIA RAIADSTSVT QTAAVIGTAQ YLSPEQARGD SVDARSDVYS 200
LGCVLYEILT GEPPFIGDSP VSVAYQHVRE DPIPPSQRHE GISVDLDAVV 250
LKALAKNPEN RYQTAAEMRA DLIRVHSGQP PEAPKVLTDA DRSCLLSSGA 300
GNFGVPRTDA LSRQSLDETE SDGSIGRWVA VVAVLAVLTI AIVAAFNTFG 350
GNTRDVQVPD VRGQVSADAI SALQNRGFKT RTLQKPDSTI PPDHVISTEP 400
GANASVGAGD EITINVSTGP EQREVPDVSS LNYTDAVKKL TSSGFKSFKQ 450
ANSPSTPELL GKVIGTNPSA NQTSAITNVI TIIVGSGPET KQIPDVTGQI 500
VEIAQKNLNV YGFTKFSQAS VDSPRPTGEV IGTNPPKDAT VPVDSVIELQ 550
VSKGNQFVMP DLSGMFWADA EPRLRALGWT GVLDKGPDVD AGGSQHNRVA 600
YQNPPAGAGV NRDGIITLKF GQ 622
Length:622
Mass (Da):66,187
Last modified:April 27, 2001 - v3
Checksum:i9611B0FD93F2BA7C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti303 – 3042FG → LC in CAA94718. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z70722 Genomic DNA. Translation: CAA94718.1.
AL583917 Genomic DNA. Translation: CAC29524.1.
PIRiH86910.
T10009.
RefSeqiNP_301142.1. NC_002677.1.
WP_010907467.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29524; CAC29524; CAC29524.
GeneIDi908655.
KEGGimle:ML0016.
PATRICi18050124. VBIMycLep78757_0020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z70722 Genomic DNA. Translation: CAA94718.1 .
AL583917 Genomic DNA. Translation: CAC29524.1 .
PIRi H86910.
T10009.
RefSeqi NP_301142.1. NC_002677.1.
WP_010907467.1. NC_002677.1.

3D structure databases

ProteinModelPortali P54744.
SMRi P54744. Positions 1-285.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML0016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC29524 ; CAC29524 ; CAC29524 .
GeneIDi 908655.
KEGGi mle:ML0016.
PATRICi 18050124. VBIMycLep78757_0020.

Organism-specific databases

Lepromai ML0016.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000037185.
KOi K08884.
OMAi AINFLES.
OrthoDBi EOG6B35XT.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene arrangement and organization in an approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae."
    Fsihi H., de Rossi E., Salazar L., Cantoni R., Labo M., Riccardi G., Takiff H.E., Eiglmeier K., Bergh S., Cole S.T.
    Microbiology 142:3147-3161(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiPKNB_MYCLE
AccessioniPrimary (citable) accession number: P54744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 27, 2001
Last modified: September 3, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi