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P54744

- PKNB_MYCLE

UniProt

P54744 - PKNB_MYCLE

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Protein

Serine/threonine-protein kinase PknB

Gene

pknB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATPPROSITE-ProRule annotation
Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation
Metal bindingi143 – 1431MagnesiumBy similarity
Metal bindingi156 – 1561MagnesiumBy similarity
Binding sitei156 – 1561ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPPROSITE-ProRule annotation
Nucleotide bindingi93 – 953ATPPROSITE-ProRule annotation
Nucleotide bindingi140 – 1434ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. penicillin binding Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PknB (EC:2.7.11.1)
Gene namesi
Name:pknB
Ordered Locus Names:ML0016
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML0016.

Subcellular locationi

Cell membrane By similarity; Single-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 328328CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei329 – 34921HelicalSequence AnalysisAdd
BLAST
Topological domaini350 – 622273ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Serine/threonine-protein kinase PknBPRO_0000171207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei166 – 1661Phosphoserine; by autocatalysisBy similarity
Modified residuei168 – 1681Phosphoserine; by autocatalysisBy similarity
Modified residuei170 – 1701Phosphothreonine; by autocatalysisBy similarity
Modified residuei172 – 1721Phosphothreonine; by autocatalysisBy similarity
Modified residuei308 – 3081Phosphothreonine; by autocatalysisBy similarity

Post-translational modificationi

Autophosphorylated. Dephosphorylated by PstP (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272631.ML0016.

Structurei

3D structure databases

ProteinModelPortaliP54744.
SMRiP54744. Positions 1-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 273263Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini352 – 41867PASTA 1PROSITE-ProRule annotationAdd
BLAST
Domaini419 – 48668PASTA 2PROSITE-ProRule annotationAdd
BLAST
Domaini487 – 55367PASTA 3PROSITE-ProRule annotationAdd
BLAST
Domaini554 – 62269PASTA 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 4 PASTA domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000037185.
KOiK08884.
OMAiAINFLES.
OrthoDBiEOG6B35XT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54744-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTPPHLSDR YELGDILGFG GMSEVHLARD IRLHRDVAVK VLRADLARDP
60 70 80 90 100
SFYLRFRREA QNAAALNHPS IVAVYDTGEA ETSAGPLPYI VMEYVDGATL
110 120 130 140 150
RDIVHTDGPM PPQQAIEIVA DACQALNFSH QNGIIHRDVK PANIMISATN
160 170 180 190 200
AVKVMDFGIA RAIADSTSVT QTAAVIGTAQ YLSPEQARGD SVDARSDVYS
210 220 230 240 250
LGCVLYEILT GEPPFIGDSP VSVAYQHVRE DPIPPSQRHE GISVDLDAVV
260 270 280 290 300
LKALAKNPEN RYQTAAEMRA DLIRVHSGQP PEAPKVLTDA DRSCLLSSGA
310 320 330 340 350
GNFGVPRTDA LSRQSLDETE SDGSIGRWVA VVAVLAVLTI AIVAAFNTFG
360 370 380 390 400
GNTRDVQVPD VRGQVSADAI SALQNRGFKT RTLQKPDSTI PPDHVISTEP
410 420 430 440 450
GANASVGAGD EITINVSTGP EQREVPDVSS LNYTDAVKKL TSSGFKSFKQ
460 470 480 490 500
ANSPSTPELL GKVIGTNPSA NQTSAITNVI TIIVGSGPET KQIPDVTGQI
510 520 530 540 550
VEIAQKNLNV YGFTKFSQAS VDSPRPTGEV IGTNPPKDAT VPVDSVIELQ
560 570 580 590 600
VSKGNQFVMP DLSGMFWADA EPRLRALGWT GVLDKGPDVD AGGSQHNRVA
610 620
YQNPPAGAGV NRDGIITLKF GQ
Length:622
Mass (Da):66,187
Last modified:April 27, 2001 - v3
Checksum:i9611B0FD93F2BA7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti303 – 3042FG → LC in CAA94718. (PubMed:8969512)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70722 Genomic DNA. Translation: CAA94718.1.
AL583917 Genomic DNA. Translation: CAC29524.1.
PIRiH86910.
T10009.
RefSeqiNP_301142.1. NC_002677.1.
WP_010907467.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29524; CAC29524; CAC29524.
GeneIDi908655.
KEGGimle:ML0016.
PATRICi18050124. VBIMycLep78757_0020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z70722 Genomic DNA. Translation: CAA94718.1 .
AL583917 Genomic DNA. Translation: CAC29524.1 .
PIRi H86910.
T10009.
RefSeqi NP_301142.1. NC_002677.1.
WP_010907467.1. NC_002677.1.

3D structure databases

ProteinModelPortali P54744.
SMRi P54744. Positions 1-285.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML0016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC29524 ; CAC29524 ; CAC29524 .
GeneIDi 908655.
KEGGi mle:ML0016.
PATRICi 18050124. VBIMycLep78757_0020.

Organism-specific databases

Lepromai ML0016.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000037185.
KOi K08884.
OMAi AINFLES.
OrthoDBi EOG6B35XT.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene arrangement and organization in an approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae."
    Fsihi H., de Rossi E., Salazar L., Cantoni R., Labo M., Riccardi G., Takiff H.E., Eiglmeier K., Bergh S., Cole S.T.
    Microbiology 142:3147-3161(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiPKNB_MYCLE
AccessioniPrimary (citable) accession number: P54744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 27, 2001
Last modified: October 29, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3