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P54744

- PKNB_MYCLE

UniProt

P54744 - PKNB_MYCLE

Protein

Serine/threonine-protein kinase PknB

Gene

pknB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 3 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401ATPPROSITE-ProRule annotation
    Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation
    Metal bindingi143 – 1431MagnesiumBy similarity
    Metal bindingi156 – 1561MagnesiumBy similarity
    Binding sitei156 – 1561ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 259ATPPROSITE-ProRule annotation
    Nucleotide bindingi93 – 953ATPPROSITE-ProRule annotation
    Nucleotide bindingi140 – 1434ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. penicillin binding Source: InterPro
    4. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase PknB (EC:2.7.11.1)
    Gene namesi
    Name:pknB
    Ordered Locus Names:ML0016
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML0016.

    Subcellular locationi

    Cell membrane By similarity; Single-pass membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Serine/threonine-protein kinase PknBPRO_0000171207Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei166 – 1661Phosphoserine; by autocatalysisBy similarity
    Modified residuei168 – 1681Phosphoserine; by autocatalysisBy similarity
    Modified residuei170 – 1701Phosphothreonine; by autocatalysisBy similarity
    Modified residuei172 – 1721Phosphothreonine; by autocatalysisBy similarity
    Modified residuei308 – 3081Phosphothreonine; by autocatalysisBy similarity

    Post-translational modificationi

    Autophosphorylated. Dephosphorylated by PstP By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi272631.ML0016.

    Structurei

    3D structure databases

    ProteinModelPortaliP54744.
    SMRiP54744. Positions 1-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 328328CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini350 – 622273ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei329 – 34921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 273263Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini352 – 41867PASTA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini419 – 48668PASTA 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini487 – 55367PASTA 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini554 – 62269PASTA 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 4 PASTA domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000037185.
    KOiK08884.
    OMAiAINFLES.
    OrthoDBiEOG6B35XT.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR005543. PASTA_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF03793. PASTA. 4 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00740. PASTA. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51178. PASTA. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54744-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTPPHLSDR YELGDILGFG GMSEVHLARD IRLHRDVAVK VLRADLARDP    50
    SFYLRFRREA QNAAALNHPS IVAVYDTGEA ETSAGPLPYI VMEYVDGATL 100
    RDIVHTDGPM PPQQAIEIVA DACQALNFSH QNGIIHRDVK PANIMISATN 150
    AVKVMDFGIA RAIADSTSVT QTAAVIGTAQ YLSPEQARGD SVDARSDVYS 200
    LGCVLYEILT GEPPFIGDSP VSVAYQHVRE DPIPPSQRHE GISVDLDAVV 250
    LKALAKNPEN RYQTAAEMRA DLIRVHSGQP PEAPKVLTDA DRSCLLSSGA 300
    GNFGVPRTDA LSRQSLDETE SDGSIGRWVA VVAVLAVLTI AIVAAFNTFG 350
    GNTRDVQVPD VRGQVSADAI SALQNRGFKT RTLQKPDSTI PPDHVISTEP 400
    GANASVGAGD EITINVSTGP EQREVPDVSS LNYTDAVKKL TSSGFKSFKQ 450
    ANSPSTPELL GKVIGTNPSA NQTSAITNVI TIIVGSGPET KQIPDVTGQI 500
    VEIAQKNLNV YGFTKFSQAS VDSPRPTGEV IGTNPPKDAT VPVDSVIELQ 550
    VSKGNQFVMP DLSGMFWADA EPRLRALGWT GVLDKGPDVD AGGSQHNRVA 600
    YQNPPAGAGV NRDGIITLKF GQ 622
    Length:622
    Mass (Da):66,187
    Last modified:April 27, 2001 - v3
    Checksum:i9611B0FD93F2BA7C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti303 – 3042FG → LC in CAA94718. (PubMed:8969512)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z70722 Genomic DNA. Translation: CAA94718.1.
    AL583917 Genomic DNA. Translation: CAC29524.1.
    PIRiH86910.
    T10009.
    RefSeqiNP_301142.1. NC_002677.1.
    WP_010907467.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC29524; CAC29524; CAC29524.
    GeneIDi908655.
    KEGGimle:ML0016.
    PATRICi18050124. VBIMycLep78757_0020.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z70722 Genomic DNA. Translation: CAA94718.1 .
    AL583917 Genomic DNA. Translation: CAC29524.1 .
    PIRi H86910.
    T10009.
    RefSeqi NP_301142.1. NC_002677.1.
    WP_010907467.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali P54744.
    SMRi P54744. Positions 1-285.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML0016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC29524 ; CAC29524 ; CAC29524 .
    GeneIDi 908655.
    KEGGi mle:ML0016.
    PATRICi 18050124. VBIMycLep78757_0020.

    Organism-specific databases

    Lepromai ML0016.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000037185.
    KOi K08884.
    OMAi AINFLES.
    OrthoDBi EOG6B35XT.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR005543. PASTA_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF03793. PASTA. 4 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00740. PASTA. 4 hits.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51178. PASTA. 4 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene arrangement and organization in an approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae."
      Fsihi H., de Rossi E., Salazar L., Cantoni R., Labo M., Riccardi G., Takiff H.E., Eiglmeier K., Bergh S., Cole S.T.
      Microbiology 142:3147-3161(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.

    Entry informationi

    Entry nameiPKNB_MYCLE
    AccessioniPrimary (citable) accession number: P54744
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3