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P54744 (PKNB_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase PknB

EC=2.7.11.1
Gene names
Name:pknB
Ordered Locus Names:ML0016
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Single-pass membrane protein By similarity.

Post-translational modification

Autophosphorylated. Dephosphorylated by PstP By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 4 PASTA domains.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Serine/threonine-protein kinase PknB
PRO_0000171207

Regions

Topological domain1 – 328328Cytoplasmic Potential
Transmembrane329 – 34921Helical; Potential
Topological domain350 – 622273Extracellular Potential
Domain11 – 273263Protein kinase
Domain352 – 41867PASTA 1
Domain419 – 48668PASTA 2
Domain487 – 55367PASTA 3
Domain554 – 62269PASTA 4
Nucleotide binding17 – 259ATP By similarity
Nucleotide binding93 – 953ATP By similarity
Nucleotide binding140 – 1434ATP By similarity

Sites

Active site1381Proton acceptor By similarity
Metal binding1431Magnesium By similarity
Metal binding1561Magnesium By similarity
Binding site401ATP By similarity
Binding site1561ATP By similarity

Amino acid modifications

Modified residue1661Phosphoserine; by autocatalysis By similarity
Modified residue1681Phosphoserine; by autocatalysis By similarity
Modified residue1701Phosphothreonine; by autocatalysis By similarity
Modified residue1721Phosphothreonine; by autocatalysis By similarity
Modified residue3081Phosphothreonine; by autocatalysis By similarity

Experimental info

Sequence conflict303 – 3042FG → LC in CAA94718. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54744 [UniParc].

Last modified April 27, 2001. Version 3.
Checksum: 9611B0FD93F2BA7C

FASTA62266,187
        10         20         30         40         50         60 
MTTPPHLSDR YELGDILGFG GMSEVHLARD IRLHRDVAVK VLRADLARDP SFYLRFRREA 

        70         80         90        100        110        120 
QNAAALNHPS IVAVYDTGEA ETSAGPLPYI VMEYVDGATL RDIVHTDGPM PPQQAIEIVA 

       130        140        150        160        170        180 
DACQALNFSH QNGIIHRDVK PANIMISATN AVKVMDFGIA RAIADSTSVT QTAAVIGTAQ 

       190        200        210        220        230        240 
YLSPEQARGD SVDARSDVYS LGCVLYEILT GEPPFIGDSP VSVAYQHVRE DPIPPSQRHE 

       250        260        270        280        290        300 
GISVDLDAVV LKALAKNPEN RYQTAAEMRA DLIRVHSGQP PEAPKVLTDA DRSCLLSSGA 

       310        320        330        340        350        360 
GNFGVPRTDA LSRQSLDETE SDGSIGRWVA VVAVLAVLTI AIVAAFNTFG GNTRDVQVPD 

       370        380        390        400        410        420 
VRGQVSADAI SALQNRGFKT RTLQKPDSTI PPDHVISTEP GANASVGAGD EITINVSTGP 

       430        440        450        460        470        480 
EQREVPDVSS LNYTDAVKKL TSSGFKSFKQ ANSPSTPELL GKVIGTNPSA NQTSAITNVI 

       490        500        510        520        530        540 
TIIVGSGPET KQIPDVTGQI VEIAQKNLNV YGFTKFSQAS VDSPRPTGEV IGTNPPKDAT 

       550        560        570        580        590        600 
VPVDSVIELQ VSKGNQFVMP DLSGMFWADA EPRLRALGWT GVLDKGPDVD AGGSQHNRVA 

       610        620 
YQNPPAGAGV NRDGIITLKF GQ 

« Hide

References

« Hide 'large scale' references
[1]"Gene arrangement and organization in an approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae."
Fsihi H., de Rossi E., Salazar L., Cantoni R., Labo M., Riccardi G., Takiff H.E., Eiglmeier K., Bergh S., Cole S.T.
Microbiology 142:3147-3161(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Massive gene decay in the leprosy bacillus."
Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. expand/collapse author list , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G.
Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z70722 Genomic DNA. Translation: CAA94718.1.
AL583917 Genomic DNA. Translation: CAC29524.1.
PIRH86910.
T10009.
RefSeqNP_301142.1. NC_002677.1.

3D structure databases

ProteinModelPortalP54744.
SMRP54744. Positions 1-285.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML0016.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC29524; CAC29524; CAC29524.
GeneID908655.
KEGGmle:ML0016.
PATRIC18050124. VBIMycLep78757_0020.

Organism-specific databases

LepromaML0016.
CMRSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000037185.
KOK08884.
OMADIVGSVH.
OrthoDBEOG6B35XT.
ProtClustDBCLSK790192.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR005543. PASTA.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePKNB_MYCLE
AccessionPrimary (citable) accession number: P54744
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 27, 2001
Last modified: February 19, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families