P54743 (PKNA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PknA
EC=2.7.11.1

Gene names

Name:	pknA
Ordered Locus Names:	ML0017

Organism

Mycobacterium leprae (strain TN) [Complete proteome] [HAMAP]

Taxonomic identifier

272631 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cell membrane; Single-pass membrane protein By similarity.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Serine/threonine-protein kinase PknA		PRO_0000171204
Regions
	Topological domain	1 – 341	341	Cytoplasmic Potential
	Transmembrane	342 – 362	21	Helical; Potential
	Topological domain	363 – 437	75	Extracellular Potential
	Domain	13 – 272	260	Protein kinase
	Nucleotide binding	19 – 27	9	ATP By similarity
Sites
	Active site	141	1	Proton acceptor By similarity
	Binding site	42	1	ATP By similarity
Experimental info
	Sequence conflict	281 – 355	75	PRPNQ…ALAII → RGPTKHHLPAGPLQRPSRQA HRPEQLLLAAPRLRHLADPD RQQAEIARRRRETRSRLDNA RCSGPPGCSERWRSS in CAA94719. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54743 [UniParc]. Last modified April 27, 2001. Version 3. Checksum: F08F07240C7C29D0	FASTA	437	46,312
	10 20 30 40 50 60 MSPRIGVMLS GRYRLHRLIA TGGMGQVWEA VDSRLGRRVA VKVLKGEFSS DPEFIERFRA 70 80 90 100 110 120 EARTTAMLNH PGIASVHDYG ESHMDGEGRT AYLVMELVNG EPLNSVLKRT GRLSLRHALD 130 140 150 160 170 180 MLEQTGRALQ IAHAAGLVHR DVKPGNILIT PTGQVKITDF GIAKAVDAAP VTQTGMVMGT 190 200 210 220 230 240 AQYIAPEQAL GHDATPASDV YSLGVIGYEV VSGKRPFTGD GALTVAMKHI KEPPPPLPAD 250 260 270 280 290 300 LPPNVRELIE ITLVKNPGMR YPSGGLFAEA VAAVRAGHRP PRPNQTPSSG RASPTTIPSS 310 320 330 340 350 360 TQARAAVACG TKTPAPRRSR PSTSGNRPPP ARNTFSSGQR ALLWAAGMLG ALAIIIAVLI 370 380 390 400 410 420 VINSYAGNEQ HQPPTPTVTD TGTPPATKTL SGFPAAYCEY RVNWTNHKEI SNSGLPKQAA 430 RAQLAGATDI SPVAGQT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene arrangement and organization in an approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae." Fsihi H., de Rossi E., Salazar L., Cantoni R., Labo M., Riccardi G., Takiff H.E., Eiglmeier K., Bergh S., Cole S.T. Microbiology 142:3147-3161(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70722 Genomic DNA. Translation: CAA94719.1. AL583917 Genomic DNA. Translation: CAC29525.1.
PIR	A86911. T10010.
RefSeq	NP_301143.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P54743.
ModBase	Search...
Protein-protein interaction databases
STRING	272631.ML0017.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC29525; CAC29525; CAC29525.
GeneID	908171.
KEGG	mle:ML0017.
PATRIC	18050126. VBIMycLep78757_0021.
Organism-specific databases
Leproma	ML0017.
CMR	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000037187.
KO	K08884.
OMA	PESIPRG.
ProtClustDB	CLSK790193.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKNA_MYCLE

Accession

Primary (citable) accession number: P54743
Secondary accession number(s): Q50184

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program