ID   AFSK_STRGR              Reviewed;         807 AA.
AC   P54742;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=Serine/threonine-protein kinase afsK;
DE            EC=2.7.11.1;
GN   Name=afsK;
OS   Streptomyces griseus.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=96186909; PubMed=8635757; DOI=10.1016/0378-1119(95)00771-7;
RA   Ueda K., Umeyama T., Beppu T., Horinouchi S.;
RT   "The aerial mycelium-defective phenotype of Streptomyces griseus
RT   resulting from A-factor deficiency is suppressed by a Ser/Thr kinase
RT   of S. coelicolor A3(2).";
RL   Gene 169:91-95(1996).
CC   -!- FUNCTION: Component of the afsK/afsR system involved in the
CC       response of aerial mycelium formation to glucose.
CC   -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
CC   -!- SUBUNIT: Interacts (via the N-terminal kinase domain) with kbpA;
CC       the interaction prevents autophosphorylation of afsK (By
CC       similarity).
CC   -!- PTM: Autophosphorylated mainly on threonine residues. Some
CC       phosphorylation on serine residues. Autophosphorylation on Thr-168
CC       is the major site enhancing kinase activity towards afsR, and is
CC       regulated though interaction with kbpA (By similarity).
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr
CC       protein kinase family.
CC   -!- SIMILARITY: Contains 1 protein kinase domain.
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DR   EMBL; D45246; BAA08203.1; -; Genomic_DNA.
DR   HSSP; P71584; 1MRU.
DR   PhosSite; P54742; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006468; P:protein amino acid phosphorylation; IEA:InterPro.
DR   InterPro; IPR018391; PQQ_beta_propeller_repeat.
DR   InterPro; IPR002372; PQQ_repeat.
DR   InterPro; IPR000719; Prot_kinase_core.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR011047; Quino_AlcDH-like.
DR   InterPro; IPR017442; Se/Thr_pkinase-rel.
DR   InterPro; IPR008271; Ser_thr_pkin_AS.
DR   Gene3D; G3DSA:2.140.10.10; Quinoprotein_alc_DH-like; 2.
DR   Pfam; PF00069; Pkinase; 1.
DR   Pfam; PF01011; PQQ; 3.
DR   ProDom; PD000001; Prot_kinase; 1.
DR   SMART; SM00564; PQQ; 8.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Nucleotide-binding; Phosphoprotein;
KW   Serine/threonine-protein kinase; Transferase.
FT   CHAIN         1    807       Serine/threonine-protein kinase afsK.
FT                                /FTId=PRO_0000171235.
FT   DOMAIN       16    272       Protein kinase.
FT   NP_BIND      22     30       ATP (By similarity).
FT   ACT_SITE    138    138       Proton acceptor (By similarity).
FT   BINDING      44     44       ATP (By similarity).
FT   MOD_RES      71     71       Phosphoserine; by autocatalysis (By
FT                                similarity).
FT   MOD_RES     168    168       Phosphothreonine; by autocatalysis (By
FT                                similarity).
SQ   SEQUENCE   807 AA;  85232 MW;  66C274219155D091 CRC64;
     MVEQLTQHDP RRIGPFEVLG RLGAGGMGLV YLARSASGRR VAIKTVRTEL AEDQLFRVRF
     TREVEAARAV SGFYTAAVVD ADPRAAVPWL ATAYVPAPSL EEIVNECGPM PTQAVRWLAA
     GIAQALQSIH GAGLVHRDLK PSNVLVVEDG PRVIDFGIAS GVSNTRLTMT NVAVGTPAYM
     SPEQARDSRS VTGASDIFSL GSTLVFAATG HAPFHGANPV ETVFMLVREG PDLEGLPDDL
     RPLIESCMQM DATHPAAEPR DLQAQLAPHL FASGSDDSGT ASAWLPVPAT AMIERRRGGR
     RTARRPPRPR PRRLRAAPQG PGAGHRLAQR GRPAFALPAV LALAAVRRVR TAAGPSAAPD
     GGPVQLPGAK VPIGPGRRAG EGRGAAAAAP RRRDRLGPAA RRSERFLGGH GPHRTVPAST
     LRPGTPSPAP DRWRPWRFRM SNDVWGTPVV SGDLLYVTSF EVHALDVGNG RRQFKTRDVA
     WAMAVEGGRI HASDGPSLYA LDAASGAEQW RLATDAWVYA LKADRGTVLT ATRGGGVQGW
     EASNGEKLWE VTGAQSDFET AEAGPVIHDG TVYLWQDARL RALDARTGLE RWSYPIGDAA
     SCGGVPVRVT PATDGYVYVA AGTRVLAVET GSGPVRWHFE APAVFLSPPA FAPGPAVTGG
     GVYLADYLGT VYALDATTGK DRWRIATEAR SSIEPVLVAV GNVHVGSGSA LYTLDAVTDT
     PKWRFAAGGD VVGAPWWRRP GPLRLGGPRA LHPGRGGRPA ALKLATGGEI TGSPVAQAGV
     VYACSKDRCV YALDALKGTG TGNRART
//