Reviewed, UniProtKB/Swiss-Prot P54742 (AFSK_STRGR)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase afsK
    EC=2.7.11.1

Gene names

Name:

afsK

Organism

Streptomyces griseus

Taxonomic identifier

1911 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the afsK/afsR system involved in the response of aerial mycelium formation to glucose.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts (via the N-terminal kinase domain) with kbpA; the interaction prevents autophosphorylation of afsK By similarity.
Post-translational modification	Autophosphorylated mainly on threonine residues. Some phosphorylation on serine residues. Autophosphorylation on Thr-168 is the major site enhancing kinase activity towards afsR, and is regulated though interaction with kbpA By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Serine/threonine-protein kinase afsK		PRO_0000171235
Regions
	Domain	16 – 272	257	Protein kinase
	Nucleotide binding	22 – 30	9	ATP By similarity
Sites
	Active site	138	1	Proton acceptor By similarity
	Binding site	44	1	ATP By similarity
Amino acid modifications
	Modified residue	71	1	Phosphoserine; by autocatalysis By similarity
	Modified residue	168	1	Phosphothreonine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54742-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 66C274219155D091	FASTA	807	85,232
	10 20 30 40 50 60 MVEQLTQHDP RRIGPFEVLG RLGAGGMGLV YLARSASGRR VAIKTVRTEL AEDQLFRVRF 70 80 90 100 110 120 TREVEAARAV SGFYTAAVVD ADPRAAVPWL ATAYVPAPSL EEIVNECGPM PTQAVRWLAA 130 140 150 160 170 180 GIAQALQSIH GAGLVHRDLK PSNVLVVEDG PRVIDFGIAS GVSNTRLTMT NVAVGTPAYM 190 200 210 220 230 240 SPEQARDSRS VTGASDIFSL GSTLVFAATG HAPFHGANPV ETVFMLVREG PDLEGLPDDL 250 260 270 280 290 300 RPLIESCMQM DATHPAAEPR DLQAQLAPHL FASGSDDSGT ASAWLPVPAT AMIERRRGGR 310 320 330 340 350 360 RTARRPPRPR PRRLRAAPQG PGAGHRLAQR GRPAFALPAV LALAAVRRVR TAAGPSAAPD 370 380 390 400 410 420 GGPVQLPGAK VPIGPGRRAG EGRGAAAAAP RRRDRLGPAA RRSERFLGGH GPHRTVPAST 430 440 450 460 470 480 LRPGTPSPAP DRWRPWRFRM SNDVWGTPVV SGDLLYVTSF EVHALDVGNG RRQFKTRDVA 490 500 510 520 530 540 WAMAVEGGRI HASDGPSLYA LDAASGAEQW RLATDAWVYA LKADRGTVLT ATRGGGVQGW 550 560 570 580 590 600 EASNGEKLWE VTGAQSDFET AEAGPVIHDG TVYLWQDARL RALDARTGLE RWSYPIGDAA 610 620 630 640 650 660 SCGGVPVRVT PATDGYVYVA AGTRVLAVET GSGPVRWHFE APAVFLSPPA FAPGPAVTGG 670 680 690 700 710 720 GVYLADYLGT VYALDATTGK DRWRIATEAR SSIEPVLVAV GNVHVGSGSA LYTLDAVTDT 730 740 750 760 770 780 PKWRFAAGGD VVGAPWWRRP GPLRLGGPRA LHPGRGGRPA ALKLATGGEI TGSPVAQAGV 790 800 VYACSKDRCV YALDALKGTG TGNRART

References

[1]	"The aerial mycelium-defective phenotype of Streptomyces griseus resulting from A-factor deficiency is suppressed by a Ser/Thr kinase of S. coelicolor A3(2)." Ueda K., Umeyama T., Beppu T., Horinouchi S. Gene 169:91-95(1996) [PubMed: 8635757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D45246 Genomic DNA. Translation: BAA08203.1.
3D structure databases
HSSP	HSSP built from PDB template 1MRU based on UniProtKB P71584.
ModBase	Search...
PTM databases
PhosSite	P54742.
Family and domain databases
InterPro	IPR018391. PQQ_beta_propeller_repeat. IPR002372. PQQ_repeat. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR011047. Quino_AlcDH-like. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Gene3D	G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 2 hits.
Pfam	PF00069. Pkinase. 1 hit. PF01011. PQQ. 3 hits. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00564. PQQ. 8 hits. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFSK_STRGR

Accession

Primary (citable) accession number: P54742

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)