##gff-version 3
P54741	UniProtKB	Chain	1	799	.	.	.	ID=PRO_0000171234;Note=Serine/threonine-protein kinase AfsK	
P54741	UniProtKB	Domain	16	271	.	.	.	Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159	
P54741	UniProtKB	Region	295	343	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P54741	UniProtKB	Region	393	426	.	.	.	Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P54741	UniProtKB	Compositional bias	323	343	.	.	.	Note=Pro residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P54741	UniProtKB	Compositional bias	407	423	.	.	.	Note=Pro residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite	
P54741	UniProtKB	Active site	138	138	.	.	.	Note=Proton acceptor;Ontology_term=ECO:0000255,ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159,ECO:0000255|PROSITE-ProRule:PRU10027	
P54741	UniProtKB	Binding site	22	30	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159	
P54741	UniProtKB	Binding site	44	44	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159	
P54741	UniProtKB	Modified residue	71	71	.	.	.	Note=Phosphoserine%3B by autocatalysis;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16629414;Dbxref=PMID:16629414	
P54741	UniProtKB	Modified residue	168	168	.	.	.	Note=Phosphothreonine%3B by autocatalysis;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16629414;Dbxref=PMID:16629414	
P54741	UniProtKB	Mutagenesis	44	44	.	.	.	Note=No autophosphorylation. Binds KbpA. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11544211;Dbxref=PMID:11544211	
P54741	UniProtKB	Mutagenesis	71	71	.	.	.	Note=No autophosphorylation. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16629414;Dbxref=PMID:16629414	
P54741	UniProtKB	Mutagenesis	128	128	.	.	.	Note=No change in autophosphorylation. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16629414;Dbxref=PMID:16629414	
P54741	UniProtKB	Mutagenesis	168	168	.	.	.	Note=Almost completely abolishes autophosphorylation. No enhancement of kinase activity on AfsR. Very little constitutive kinase activity. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16629414;Dbxref=PMID:16629414	
P54741	UniProtKB	Mutagenesis	168	168	.	.	.	Note=Almost completely abolishes autophosphorylation. No enhancement of kinase activity on AfsR. Constitutive kinase activity. T->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16629414;Dbxref=PMID:16629414	
P54741	UniProtKB	Mutagenesis	168	168	.	.	.	Note=Almost completely abolishes autophosphorylation. No enhancement of kinase activity on AfsR. Some constitutive kinase activity. T->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16629414;Dbxref=PMID:16629414	
P54741	UniProtKB	Mutagenesis	170	170	.	.	.	Note=No change in autophosphorylation. T->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16629414;Dbxref=PMID:16629414