P54740 (PKAB_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PkaB
EC=2.7.11.1

Gene names

Name:	pkaB
Ordered Locus Names:	SCO2973
ORF Names:	SCE50.01, SCE59.32c

Organism

Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]

Taxonomic identifier

100226 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces coelicolor

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Autophosphorylated mainly at Thr.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine/threonine-protein kinase PkaB		PRO_0000171233
Regions
	Domain	9 – 270	262	Protein kinase
	Nucleotide binding	15 – 23	9	ATP By similarity
Sites
	Active site	130	1	Proton acceptor By similarity
	Binding site	36	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54740 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3D66720FF17D3DE4	FASTA	417	43,417
	10 20 30 40 50 60 MARKIGSRYT AHQILGRGSA GTVWLGEGPD GPVAIKLLRE DLASDQELVS RFVQERTALL 70 80 90 100 110 120 GLDHPHVVSV RDLVVDGNDL ALVMDLVRGT DLRTRLDRER RLAPEAAVAV VADVADGLAA 130 140 150 160 170 180 AHAAGVVHRD VKPENVLLDM QGPLGPGGSH PALLTDFGVA KLIDTPRRTR ATKIIGTPDY 190 200 210 220 230 240 LAPEIVEGLP PRAAVDIYAL ATVLYELLAG FTPFGGGHPG AVLRRHVTET VVPLPGIPDE 250 260 270 280 290 300 LWQLLVQCLA KAPASRLRAS ELSARLRELL PMLAGMAPLD VDEPDAEQPE DAPDASAASP 310 320 330 340 350 360 AAPVSTAEPV RRRGAVPLVP GAKPADSNRD THTSMRVPAP DELAGGARGT ARAPRASGAP 370 380 390 400 410 RPGSARNRAA TRRRRIAVGA GAVALVAAIG VGTWLATGGD EDGGGPQDTR NSAPAAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and expression of serine/threonine kinase-encoding genes from Streptomyces coelicolor A3(2)." Urabe H., Ogawara H. Gene 153:99-104(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2) / NRRL B-16638.
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86821 Genomic DNA. Translation: BAA13169.1. AL939114 Genomic DNA. Translation: CAD55460.1.
PIR	JC4071.
RefSeq	NP_733588.1. NC_003888.3.
3D structure databases
ProteinModelPortal	P54740.
ModBase	Search...
Protein-protein interaction databases
STRING	100226.SCO2973.
PTM databases
PhosSite	P0502123.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD55460; CAD55460; CAD55460.
GeneID	1098406.
KEGG	sco:SCO2973.
PATRIC	23735672. VBIStrCoe124346_3031.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000053674.
OMA	RRHVTET.
ProtClustDB	CLSK392718.
Enzyme and pathway databases
BRENDA	2.7.11.1. 5998.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKAB_STRCO

Accession

Primary (citable) accession number: P54740
Secondary accession number(s): Q9L061, Q9L1S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program