P54734 (PKNA_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PknA
EC=2.7.11.1

Gene names

Name:	pknA
Ordered Locus Names:	alr4366

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probably required for both normal cellular growth and differentiation. Inactivation of pknA leads to colonies that appear light green and rough in the absence of combined nitrogen.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Serine/threonine-protein kinase PknA		PRO_0000171181
Regions
	Domain	9 – 271	263	Protein kinase
	Nucleotide binding	15 – 23	9	ATP By similarity
Sites
	Active site	139	1	Proton acceptor By similarity
	Binding site	40	1	ATP By similarity
Experimental info
	Sequence conflict	265	1	E → Q in AAB41116. Ref.1
	Sequence conflict	522	1	A → R in AAB41116. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54734 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: AD0485E7AC69E785	FASTA	564	62,385
	10 20 30 40 50 60 MEQLLDNRYR VIKTLGSGGF GETFLAEDSQ MPSNRRCVVK QLRPIHNNPQ IYQLVQERFQ 70 80 90 100 110 120 REAAILEDLG SYSGQIPTLY AYFQSNTQFY VVQEWVEGDT LTAKLKQQGV LSESAVRDIL 130 140 150 160 170 180 INLLPVLEYV HSKRIIHRDI KPDNIILRHR DGKPVLIDFG AVRESMGTVI NSQGNPTSSI 190 200 210 220 230 240 VIGTPGYMPS EQAAGRPVYS SDLYSLGLTA IYLLTGKQPQ ELETEPHSGE IIWHRYALNI 250 260 270 280 290 300 SPTLAAVIDR AIAYHPRERF TTAREMLEAL QLGVVSYPPT VPYQQPQSSP TVPYQQPPVV 310 320 330 340 350 360 TTPPFATQTN TVAVSPGTAP TPQPINHNNS NKGILMGSLI AGGLIGASVV IGFALTRPNQ 370 380 390 400 410 420 PVTQTTSLPS ETTISNNDTP TVEPSPTDTP ETPISQTVTQ DPTPQASVRF PINSRPFTTP 430 440 450 460 470 480 IDSKPRNTTE PTTSVPQPTT PSEPQITTPV EATDRPSPEQ AVQNYYETIN QGEYSTAWNL 490 500 510 520 530 540 LASSFQNNRK LHPRGYDSYL DWWGGQVENV DVEQVSLLKA NADTATVNAR LRYFMKSGRQ 550 560 SSSSVRFSLV WDADNNRWVV SGAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene encoding a protein related to eukaryotic protein kinases from the filamentous heterocystous cyanobacterium Anabaena PCC 7120." Zhang C.C. Proc. Natl. Acad. Sci. U.S.A. 90:11840-11844(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00484 Genomic DNA. Translation: AAB41116.1. BA000019 Genomic DNA. Translation: BAB76065.1.
PIR	AF2351.
RefSeq	NP_488406.1. NC_003272.1.
3D structure databases
ProteinModelPortal	P54734.
ModBase	Search...
Protein-protein interaction databases
STRING	103690.alr4366.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB76065; BAB76065; BAB76065.
GeneID	1107967.
KEGG	ana:alr4366.
PATRIC	22779306. VBINosSp37423_5100.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000035389.
KO	K08884.
OMA	IDITIAK.
ProtClustDB	CLSK2393641.
Enzyme and pathway databases
BRENDA	2.7.11.1. 319.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKNA_NOSS1

Accession

Primary (citable) accession number: P54734

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program