P54730 (UBX4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UBX domain-containing protein 4

Gene names

Name:	UBX4
Ordered Locus Names:	YMR067C
ORF Names:	YM9916.06C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in CDC48-dependent protein degradation through the ubiquitin/proteasome pathway By similarity.
Subcellular location	Nucleus. Cytoplasm Ref.3.
Miscellaneous	Present with 3570 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 UBX domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER-associated protein catabolic process Inferred from genetic interaction. Source: SGD sporulation resulting in formation of a cellular spore Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.3. Source: SGD nucleus Inferred from direct assay Ref.3. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC48	P25694	5	EBI-28127,EBI-4308

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	UBX domain-containing protein 4		PRO_0000211001
Regions
	Domain	273 – 350	78	UBX
Amino acid modifications
	Modified residue	344	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P54730 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 615E5F8EC1DCDD31	FASTA	416	46,965
	10 20 30 40 50 60 MPMVTVKYNF QLFKCKVSLN STLNDVLHQS IQFFQLHTSS NDWSLIHLDK PVPLDLPWRL 70 80 90 100 110 120 LNLPTGVNLE LSKSSNFPVA NKTNREDIPF NTIKIRFQIP GRDSVVKEMP SDQPIAPILR 130 140 150 160 170 180 QMSGAAGDDF KIQVFSKIIE FKTIKDENLT LENLGIQEPS SVRLIFNNTS HSEGISANSA 190 200 210 220 230 240 IHPKQTPPTM TNPETVASLP PHELHKPSVF LPSDEPLAVI KDQIEDEEDY ELTVEQAKKY 250 260 270 280 290 300 QKMLSSKAGT LGGPILTKRL REQSANNLPK KNKAISECLL RVKFPDRSHI QIAFKPNEDM 310 320 330 340 350 360 RTVYNVVSQF LIDENMPFTL NQSHPFKPLA KDDKKLLDDL EFGSKTMLLF ETNSNSNGPL 370 380 390 400 410 IKAHLLEDAQ KITHETRTTP SVNTINKSNP QGPSDNATSI KKTLNRVPKW MKLSKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Shp1 and Ubx2 are adaptors of Cdc48 involved in ubiquitin-dependent protein degradation." Schuberth C., Richly H., Rumpf S., Buchberger A. EMBO Rep. 5:818-824(2004) [PubMed: 15258615] [Abstract] Cited for: INTERACTION WITH CDC48.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-344, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48952 Genomic DNA. Translation: CAA88792.1. BK006946 Genomic DNA. Translation: DAA09965.1.
PIR	S52827.
RefSeq	NP_013783.1. NM_001182565.1.
3D structure databases
ProteinModelPortal	P54730.
SMR	P54730. Positions 2-78.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5070N.
IntAct	P54730. 2 interactions.
MINT	MINT-491552.
STRING	P54730.
Proteomic databases
PeptideAtlas	P54730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR067C; YMR067C; YMR067C.
GeneID	855089.
KEGG	sce:YMR067C.
NMPDR	fig|4932.3.peg.4830.
Organism-specific databases
CYGD	YMR067c.
SGD	S000004671. UBX4.
Phylogenomic databases
eggNOG	fuNOG11784.
HOGENOM	HBG203407.
OMA	PHELHKP.
OrthoDB	EOG4J6W1B.
Gene expression databases
ArrayExpress	P54730.
Genevestigator	P54730.
GermOnline	YMR067C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021569. TUG. IPR001012. UBX. [Graphical view]
Pfam	PF11470. TUG. 1 hit. PF00789. UBX. 1 hit. [Graphical view]
SMART	SM00166. UBX. 1 hit. [Graphical view]
PROSITE	PS50033. UBX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978390.

Entry information

Entry name

UBX4_YEAST

Accession

Primary (citable) accession number: P54730
Secondary accession number(s): D6VZP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program