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Protein

NEDD8 ultimate buster 1

Gene

Nub1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8 ultimate buster 1
Alternative name(s):
Negative regulator of ubiquitin-like proteins 1
Protein BS4
Gene namesi
Name:Nub1
Synonyms:Nyren18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1889001. Nub1.

Subcellular locationi

  • Nucleus By similarity

  • Note: Predominantly nuclear.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002109931 – 614NEDD8 ultimate buster 1Add BLAST614

Proteomic databases

EPDiP54729.
PaxDbiP54729.
PeptideAtlasiP54729.
PRIDEiP54729.

PTM databases

iPTMnetiP54729.
PhosphoSitePlusiP54729.

Expressioni

Developmental stagei

Strongest expression at 7 dpc. Marked decrease 11, 15 and 17 dpc.

Inductioni

By interferon.

Gene expression databases

BgeeiENSMUSG00000028954.
CleanExiMM_NUB1.
ExpressionAtlasiP54729. baseline and differential.
GenevisibleiP54729. MM.

Interactioni

Subunit structurei

Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates (By similarity).By similarity

Protein-protein interaction databases

BioGridi207276. 1 interactor.
STRINGi10090.ENSMUSP00000070265.

Structurei

Secondary structure

1614
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi484 – 486Combined sources3
Helixi491 – 500Combined sources10
Helixi504 – 513Combined sources10
Turni514 – 516Combined sources3
Helixi518 – 528Combined sources11
Beta strandi536 – 538Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEGNMR-A469-538[»]
ProteinModelPortaliP54729.
SMRiP54729.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54729.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini373 – 413UBA 1PROSITE-ProRule annotationAdd BLAST41
Domaini423 – 469UBA 2PROSITE-ProRule annotationAdd BLAST47
Domaini488 – 528UBA 3PROSITE-ProRule annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni426 – 473NEDD8-binding 1By similarityAdd BLAST48
Regioni549 – 597NEDD8-binding 2By similarityAdd BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili36 – 71Sequence analysisAdd BLAST36
Coiled coili151 – 206Sequence analysisAdd BLAST56

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi413 – 430Nuclear localization signalCuratedAdd BLAST18

Sequence similaritiesi

Contains 3 UBA domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG2561. Eukaryota.
ENOG410ZK3P. LUCA.
GeneTreeiENSGT00390000010557.
HOGENOMiHOG000113882.
HOVERGENiHBG045600.
InParanoidiP54729.
PhylomeDBiP54729.
TreeFamiTF323449.

Family and domain databases

InterProiIPR011990. TPR-like_helical_dom.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00627. UBA. 3 hits.
[Graphical view]
SMARTiSM00165. UBA. 3 hits.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 3 hits.
SSF48452. SSF48452. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54729-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKKYLQAK LTQFLREDRI QLWKPPYTKE NKEVGLAVKD LAKKYSERLE
60 70 80 90 100
CCENEVENII EEIRRKAIER GTGNEHYRTT GIATIEVFLP PRLRKHDKKS
110 120 130 140 150
LLETRLHVTG RDLRCQIAET FGFQENYIKI VINKKQLQLG KSLEEQGVTH
160 170 180 190 200
NVKAMVLELK QSEEDVRKNL QLEEEEQNEA ELKERRIQRT KRGLEILAER
210 220 230 240 250
AEMVVDPETM PYLDIANQTG RSLRIPPAER KALMLAMGYH EKGRAFLKRK
260 270 280 290 300
EYGIALPCLL DADRYFCECK ELLDTVDNYA VLQLDIVWCY FRLEQLECLD
310 320 330 340 350
DAEKKLNLAQ KCFKNCYGEN HQRLVHIKGN CGKEKVLFLR LYLLQGIQNY
360 370 380 390 400
HSGNGEEARE YLNKARQLFK ELYIDPSKVH NLLQLGFTAQ EARLGLRACD
410 420 430 440 450
GNVDHAATHI SNRREELAQI RKEEKEKRRR RLENVNTLRG MGYSTQAAKQ
460 470 480 490 500
ALHQARGNLD DALKVLLSNP HMWWLQDADP ENNSRQASPS QESINQLVYM
510 520 530 540 550
GFDTVVAEAA LRVFGGNVQL AAQTLAHHGG SLPPDLQFSG EDSSPTPSTS
560 570 580 590 600
PSDSAGTSSA STDEDMETEA VNEILEDIPE HEEDYLDSTL EDEEVIIAEY
610
LSYVESISSA AKNN
Length:614
Mass (Da):70,307
Last modified:September 26, 2003 - v2
Checksum:i8287222F141D1B80
GO

Sequence cautioni

The sequence AAA68612 differs from that shown. Reason: Frameshift at position 611.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27462 mRNA. Translation: AAA68612.1. Frameshift.
AF534114 mRNA. Translation: AAM97686.1.
BC037451 mRNA. Translation: AAH37451.1.
CCDSiCCDS39030.1.
RefSeqiNP_058016.2. NM_016736.3.
UniGeneiMm.407975.
Mm.5856.

Genome annotation databases

EnsembliENSMUST00000068825; ENSMUSP00000070265; ENSMUSG00000028954.
GeneIDi53312.
KEGGimmu:53312.
UCSCiuc008wsf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27462 mRNA. Translation: AAA68612.1. Frameshift.
AF534114 mRNA. Translation: AAM97686.1.
BC037451 mRNA. Translation: AAH37451.1.
CCDSiCCDS39030.1.
RefSeqiNP_058016.2. NM_016736.3.
UniGeneiMm.407975.
Mm.5856.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEGNMR-A469-538[»]
ProteinModelPortaliP54729.
SMRiP54729.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207276. 1 interactor.
STRINGi10090.ENSMUSP00000070265.

PTM databases

iPTMnetiP54729.
PhosphoSitePlusiP54729.

Proteomic databases

EPDiP54729.
PaxDbiP54729.
PeptideAtlasiP54729.
PRIDEiP54729.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068825; ENSMUSP00000070265; ENSMUSG00000028954.
GeneIDi53312.
KEGGimmu:53312.
UCSCiuc008wsf.2. mouse.

Organism-specific databases

CTDi51667.
MGIiMGI:1889001. Nub1.

Phylogenomic databases

eggNOGiKOG2561. Eukaryota.
ENOG410ZK3P. LUCA.
GeneTreeiENSGT00390000010557.
HOGENOMiHOG000113882.
HOVERGENiHBG045600.
InParanoidiP54729.
PhylomeDBiP54729.
TreeFamiTF323449.

Miscellaneous databases

ChiTaRSiNub1. mouse.
EvolutionaryTraceiP54729.
PROiP54729.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028954.
CleanExiMM_NUB1.
ExpressionAtlasiP54729. baseline and differential.
GenevisibleiP54729. MM.

Family and domain databases

InterProiIPR011990. TPR-like_helical_dom.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00627. UBA. 3 hits.
[Graphical view]
SMARTiSM00165. UBA. 3 hits.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 3 hits.
SSF48452. SSF48452. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUB1_MOUSE
AccessioniPrimary (citable) accession number: P54729
Secondary accession number(s): Q8K3U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 26, 2003
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.