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Protein

UV excision repair protein RAD23 homolog A

Gene

Rad23a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome (By similarity).By similarity
Involved in nucleotide excision repair and is thought to be functional equivalent for Rad23b in global genome nucleotide excision repair (GG-NER) by association with Xpc. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize Xpc. Reported differences to Rad23b in regard to NER activity and Xpc stabilization are probably due to differences in expression levels with Rad23a being much less expressed than Rad23b.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-5689877. Josephin domain DUBs.

Names & Taxonomyi

Protein namesi
Recommended name:
UV excision repair protein RAD23 homolog A
Short name:
HR23A
Short name:
mHR23A
Gene namesi
Name:Rad23a
Synonyms:Mhr23a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:105126. Rad23a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Rad23a and Rad23b double knockout is embryonic lethal. Cells show reduced cell survival upopn UV radiation and reduced steady-state level of Xpc indicating a reduced NER capacity.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149051 – 363UV excision repair protein RAD23 homolog AAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei123PhosphoserineCombined sources1
Modified residuei128PhosphoserineBy similarity1
Modified residuei133PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei205PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei357PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP54726.
MaxQBiP54726.
PaxDbiP54726.
PeptideAtlasiP54726.
PRIDEiP54726.

PTM databases

iPTMnetiP54726.
PhosphoSitePlusiP54726.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003813.
CleanExiMM_RAD23A.
ExpressionAtlasiP54726. baseline and differential.
GenevisibleiP54726. MM.

Interactioni

Subunit structurei

Interacts with XPC; the interaction is suggesting the existence of a functional equivalent variant XPC complex. Interacts with PSMD4 and PSMC5. Interacts with ATXN3. Interacts with UBQLN2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202561. 1 interactor.
IntActiP54726. 1 interactor.
STRINGi10090.ENSMUSP00000003911.

Structurei

3D structure databases

ProteinModelPortaliP54726.
SMRiP54726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 79Ubiquitin-likePROSITE-ProRule annotationAdd BLAST79
Domaini161 – 201UBA 1PROSITE-ProRule annotationAdd BLAST41
Domaini318 – 358UBA 2PROSITE-ProRule annotationAdd BLAST41

Domaini

The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit Psmd4 disrupt the UBL-UBA domain interactions and drive Rad23a in to an open conformation (By similarity).By similarity

Sequence similaritiesi

Belongs to the RAD23 family.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0011. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00390000012078.
HOGENOMiHOG000172162.
HOVERGENiHBG055042.
InParanoidiP54726.
KOiK10839.
OMAiVACCVRI.
OrthoDBiEOG091G0DVL.
PhylomeDBiP54726.

Family and domain databases

Gene3Di1.10.10.540. 1 hit.
InterProiIPR004806. Rad23.
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR015360. XPC-bd.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00240. ubiquitin. 1 hit.
PF09280. XPC-binding. 1 hit.
[Graphical view]
PRINTSiPR01839. RAD23PROTEIN.
SMARTiSM00727. STI1. 1 hit.
SM00165. UBA. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF101238. SSF101238. 1 hit.
SSF46934. SSF46934. 2 hits.
SSF54236. SSF54236. 1 hit.
TIGRFAMsiTIGR00601. rad23. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTITLKTL QQQTFKIRME PDETVKVLKE KIEAEKGRDA FPVAGQKLIY
60 70 80 90 100
AGKILSDDVP IKEYHIDEKN FVVVMVTKAK AGQGIPAPPE ASPTAVPEPS
110 120 130 140 150
TPFPPVLASG MSHPPPTSRE DKSPSEESTT TTSPESISGS VPSSGSSGRE
160 170 180 190 200
EDAASTLVTG SEYETMLTEI MSMGYERERV VAALRASYNN PHRAVEYLLT
210 220 230 240 250
GIPGSPEPEH GSVQESQAPE QPATEAAGEN PLEFLRDQPQ FQNMRQVIQQ
260 270 280 290 300
NPALLPALLQ QLGQENPQLL QQISRHQEQF IQMLNEPPGE LADISDVEGE
310 320 330 340 350
VGAIGEEAPQ MNYIQVTPQE KEAIERLKAL GFPESLVIQA YFACEKNENL
360
AANFLLSQNF DDE
Length:363
Mass (Da):39,706
Last modified:July 27, 2011 - v2
Checksum:iBA129D69318BDDE7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62 – 63KE → RD in CAA63145 (PubMed:8808275).Curated2
Sequence conflicti86P → S in CAA63145 (PubMed:8808275).Curated1
Sequence conflicti218 – 219AP → RA in CAA63145 (PubMed:8808275).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92410 mRNA. Translation: CAA63145.1.
BC145372 mRNA. Translation: AAI45373.1.
CH466525 Genomic DNA. Translation: EDL10960.1.
CCDSiCCDS80908.1.
RefSeqiNP_001284535.1. NM_001297606.1.
UniGeneiMm.255539.

Genome annotation databases

EnsembliENSMUST00000109761; ENSMUSP00000105383; ENSMUSG00000003813.
GeneIDi19358.
KEGGimmu:19358.
UCSCiuc009mnm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92410 mRNA. Translation: CAA63145.1.
BC145372 mRNA. Translation: AAI45373.1.
CH466525 Genomic DNA. Translation: EDL10960.1.
CCDSiCCDS80908.1.
RefSeqiNP_001284535.1. NM_001297606.1.
UniGeneiMm.255539.

3D structure databases

ProteinModelPortaliP54726.
SMRiP54726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202561. 1 interactor.
IntActiP54726. 1 interactor.
STRINGi10090.ENSMUSP00000003911.

PTM databases

iPTMnetiP54726.
PhosphoSitePlusiP54726.

Proteomic databases

EPDiP54726.
MaxQBiP54726.
PaxDbiP54726.
PeptideAtlasiP54726.
PRIDEiP54726.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109761; ENSMUSP00000105383; ENSMUSG00000003813.
GeneIDi19358.
KEGGimmu:19358.
UCSCiuc009mnm.2. mouse.

Organism-specific databases

CTDi5886.
MGIiMGI:105126. Rad23a.

Phylogenomic databases

eggNOGiKOG0011. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00390000012078.
HOGENOMiHOG000172162.
HOVERGENiHBG055042.
InParanoidiP54726.
KOiK10839.
OMAiVACCVRI.
OrthoDBiEOG091G0DVL.
PhylomeDBiP54726.

Enzyme and pathway databases

ReactomeiR-MMU-5689877. Josephin domain DUBs.

Miscellaneous databases

PROiP54726.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003813.
CleanExiMM_RAD23A.
ExpressionAtlasiP54726. baseline and differential.
GenevisibleiP54726. MM.

Family and domain databases

Gene3Di1.10.10.540. 1 hit.
InterProiIPR004806. Rad23.
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR015360. XPC-bd.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00240. ubiquitin. 1 hit.
PF09280. XPC-binding. 1 hit.
[Graphical view]
PRINTSiPR01839. RAD23PROTEIN.
SMARTiSM00727. STI1. 1 hit.
SM00165. UBA. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF101238. SSF101238. 1 hit.
SSF46934. SSF46934. 2 hits.
SSF54236. SSF54236. 1 hit.
TIGRFAMsiTIGR00601. rad23. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRD23A_MOUSE
AccessioniPrimary (citable) accession number: P54726
Secondary accession number(s): B7ZNQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.