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Protein

UV excision repair protein RAD23 homolog A

Gene

Rad23a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome (By similarity).By similarity
Involved in nucleotide excision repair and is thought to be functional equivalent for Rad23b in global genome nucleotide excision repair (GG-NER) by association with Xpc. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize Xpc. Reported differences to Rad23b in regard to NER activity and Xpc stabilization are probably due to differences in expression levels with Rad23a being much less expressed than Rad23b.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-5696394 DNA Damage Recognition in GG-NER
R-MMU-5696395 Formation of Incision Complex in GG-NER

Names & Taxonomyi

Protein namesi
Recommended name:
UV excision repair protein RAD23 homolog A
Short name:
HR23A
Short name:
mHR23A
Gene namesi
Name:Rad23a
Synonyms:Mhr23a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:105126 Rad23a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Proteasome

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Rad23a and Rad23b double knockout is embryonic lethal. Cells show reduced cell survival upopn UV radiation and reduced steady-state level of Xpc indicating a reduced NER capacity.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149051 – 363UV excision repair protein RAD23 homolog AAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei123PhosphoserineCombined sources1
Modified residuei128PhosphoserineBy similarity1
Modified residuei133PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei205PhosphoserineBy similarity1
Modified residuei295PhosphoserineBy similarity1
Modified residuei357PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP54726
MaxQBiP54726
PaxDbiP54726
PeptideAtlasiP54726
PRIDEiP54726

PTM databases

iPTMnetiP54726
PhosphoSitePlusiP54726

Expressioni

Gene expression databases

BgeeiENSMUSG00000003813
CleanExiMM_RAD23A
ExpressionAtlasiP54726 baseline and differential
GenevisibleiP54726 MM

Interactioni

Subunit structurei

Interacts with XPC; the interaction is suggesting the existence of a functional equivalent variant XPC complex. Interacts with PSMD4 and PSMC5. Interacts with ATXN3. Interacts with UBQLN2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202561, 2 interactors
IntActiP54726, 1 interactor
STRINGi10090.ENSMUSP00000003911

Structurei

3D structure databases

ProteinModelPortaliP54726
SMRiP54726
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 79Ubiquitin-likePROSITE-ProRule annotationAdd BLAST79
Domaini161 – 201UBA 1PROSITE-ProRule annotationAdd BLAST41
Domaini318 – 358UBA 2PROSITE-ProRule annotationAdd BLAST41

Domaini

The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit Psmd4 disrupt the UBL-UBA domain interactions and drive Rad23a in to an open conformation (By similarity).By similarity

Sequence similaritiesi

Belongs to the RAD23 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0011 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00390000012078
HOGENOMiHOG000172162
HOVERGENiHBG055042
InParanoidiP54726
KOiK10839
OMAiKGRDAFP
OrthoDBiEOG091G0DVL
PhylomeDBiP54726

Family and domain databases

Gene3Di1.10.10.540, 1 hit
InterProiView protein in InterPro
IPR004806 Rad23
IPR006636 STI1_HS-bd
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR015360 XPC-bd
IPR036353 XPC-bd_sf
PfamiView protein in Pfam
PF00627 UBA, 2 hits
PF00240 ubiquitin, 1 hit
PF09280 XPC-binding, 1 hit
PRINTSiPR01839 RAD23PROTEIN
SMARTiView protein in SMART
SM00727 STI1, 1 hit
SM00165 UBA, 2 hits
SM00213 UBQ, 1 hit
SUPFAMiSSF101238 SSF101238, 1 hit
SSF46934 SSF46934, 2 hits
SSF54236 SSF54236, 1 hit
TIGRFAMsiTIGR00601 rad23, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 2 hits
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P54726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTITLKTL QQQTFKIRME PDETVKVLKE KIEAEKGRDA FPVAGQKLIY
60 70 80 90 100
AGKILSDDVP IKEYHIDEKN FVVVMVTKAK AGQGIPAPPE ASPTAVPEPS
110 120 130 140 150
TPFPPVLASG MSHPPPTSRE DKSPSEESTT TTSPESISGS VPSSGSSGRE
160 170 180 190 200
EDAASTLVTG SEYETMLTEI MSMGYERERV VAALRASYNN PHRAVEYLLT
210 220 230 240 250
GIPGSPEPEH GSVQESQAPE QPATEAAGEN PLEFLRDQPQ FQNMRQVIQQ
260 270 280 290 300
NPALLPALLQ QLGQENPQLL QQISRHQEQF IQMLNEPPGE LADISDVEGE
310 320 330 340 350
VGAIGEEAPQ MNYIQVTPQE KEAIERLKAL GFPESLVIQA YFACEKNENL
360
AANFLLSQNF DDE
Length:363
Mass (Da):39,706
Last modified:July 27, 2011 - v2
Checksum:iBA129D69318BDDE7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62 – 63KE → RD in CAA63145 (PubMed:8808275).Curated2
Sequence conflicti86P → S in CAA63145 (PubMed:8808275).Curated1
Sequence conflicti218 – 219AP → RA in CAA63145 (PubMed:8808275).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92410 mRNA Translation: CAA63145.1
BC145372 mRNA Translation: AAI45373.1
CH466525 Genomic DNA Translation: EDL10960.1
CCDSiCCDS80908.1
RefSeqiNP_001284535.1, NM_001297606.1
UniGeneiMm.255539

Genome annotation databases

EnsembliENSMUST00000109761; ENSMUSP00000105383; ENSMUSG00000003813
GeneIDi19358
KEGGimmu:19358
UCSCiuc009mnm.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRD23A_MOUSE
AccessioniPrimary (citable) accession number: P54726
Secondary accession number(s): B7ZNQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health