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Protein

UV excision repair protein RAD23 homolog A

Gene

RAD23A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.

GO - Molecular functioni

  • damaged DNA binding Source: InterPro
  • polyubiquitin binding Source: UniProtKB
  • single-stranded DNA binding Source: ProtInc
  • ubiquitin-specific protease binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • nucleotide-excision repair Source: UniProtKB
  • positive regulation of viral genome replication Source: UniProtKB
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: InterPro
  • regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Host-virus interaction

Enzyme and pathway databases

BioCyciZFISH:ENSG00000179262-MONOMER.
ReactomeiR-HSA-5689877. Josephin domain DUBs.
R-HSA-5696394. DNA Damage Recognition in GG-NER.
R-HSA-5696395. Formation of Incision Complex in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
UV excision repair protein RAD23 homolog A
Short name:
HR23A
Short name:
hHR23A
Gene namesi
Name:RAD23A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:9812. RAD23A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8K → A: No effect on interaction with EEF1A1. 1 Publication1
Mutagenesisi9T → A: Abolishes interaction with PSMD4; when associated with T-49. 1 Publication1
Mutagenesisi10L → E: Impairs UBL-UBA domain interaction and enhances ubiquitin-binding; when associated with Glu-47. 1 Publication1
Mutagenesisi47K → A: No effect on UBL-UBA domain interaction. 1 Publication1
Mutagenesisi47K → E: Impairs UBL-UBA domain interaction and enhances ubiquitin-binding; when associated with Glu-10. 1 Publication1
Mutagenesisi47K → E: Impairs UBL-UBA domain interaction and enhances ubiquitin-binding; when associated with Glu-77. 1 Publication1
Mutagenesisi49I → A: Impairs interaction with PSMD4. 2 Publications1
Mutagenesisi49I → T: Abolishes interaction with PSMD4; when associated with A-9. 2 Publications1
Mutagenesisi54I → A: Impairs interaction with PSMD4. 1 Publication1
Mutagenesisi71F → A: Impairs interaction with PSMD4. 1 Publication1
Mutagenesisi77T → E: Impairs UBL-UBA domain interaction and enhances ubiquitin-binding; when associated with Glu-47. 2 Publications1
Mutagenesisi77T → S: No effect on interaction with PSMD4. 2 Publications1
Mutagenesisi79T → P: Increases interaction with PSMD1 and PSMC1. 1 Publication1
Mutagenesisi333P → E: Abolishes interaction with HIV-1 vpr. 1 Publication1

Organism-specific databases

DisGeNETi5886.
OpenTargetsiENSG00000179262.
PharmGKBiPA34172.

Polymorphism and mutation databases

BioMutaiRAD23A.
DMDMi1709983.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149041 – 363UV excision repair protein RAD23 homolog AAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residuei123PhosphoserineCombined sources1
Modified residuei128PhosphoserineCombined sources1
Modified residuei133PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineBy similarity1
Modified residuei205PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1
Modified residuei357PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP54725.
MaxQBiP54725.
PaxDbiP54725.
PeptideAtlasiP54725.
PRIDEiP54725.

PTM databases

iPTMnetiP54725.
PhosphoSitePlusiP54725.

Miscellaneous databases

PMAP-CutDBP54725.

Expressioni

Gene expression databases

BgeeiENSG00000179262.
CleanExiHS_RAD23A.
ExpressionAtlasiP54725. baseline and differential.
GenevisibleiP54725. HS.

Organism-specific databases

HPAiHPA065599.

Interactioni

Subunit structurei

Interacts with XPC; the interaction is suggesting the existence of a functional equivalent variant XPC complex. Interacts with PSMD4 and PSMC5. Interacts with ATXN3. Interacts with HIV-1 vpr. Interacts with UBQLN2.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ANKRD40Q6AI123EBI-746453,EBI-2838246
EEF1A1P681042EBI-746453,EBI-352162
FAM188AQ9H8M75EBI-746453,EBI-724928
NGLY1Q96IV07EBI-746453,EBI-6165879
Ngly1Q9JI782EBI-746453,EBI-3648128From a different organism.
PSMD4P550363EBI-746453,EBI-359318
SQSTM1Q135012EBI-746453,EBI-307104
TRAF2Q129336EBI-746453,EBI-355744
TRAF5O004633EBI-746453,EBI-523498
TRIM54Q9BYV23EBI-746453,EBI-2130429
TRIP6Q156543EBI-746453,EBI-742327
UBQLN2Q9UHD95EBI-746453,EBI-947187
USP25Q9UHP37EBI-746453,EBI-2513462
ZBTB44Q8NCP53EBI-746453,EBI-5658292
ZBTB8AQ96BR93EBI-746453,EBI-742740

GO - Molecular functioni

  • polyubiquitin binding Source: UniProtKB
  • ubiquitin-specific protease binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi111823. 241 interactors.
DIPiDIP-34442N.
IntActiP54725. 60 interactors.
MINTiMINT-105454.
STRINGi9606.ENSP00000467024.

Structurei

Secondary structure

1363
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Turni10 – 12Combined sources3
Beta strandi14 – 19Combined sources6
Beta strandi21 – 23Combined sources3
Helixi25 – 36Combined sources12
Turni38 – 40Combined sources3
Helixi43 – 45Combined sources3
Beta strandi46 – 50Combined sources5
Beta strandi53 – 55Combined sources3
Beta strandi57 – 60Combined sources4
Helixi61 – 64Combined sources4
Beta strandi70 – 76Combined sources7
Beta strandi81 – 83Combined sources3
Beta strandi96 – 98Combined sources3
Beta strandi106 – 108Combined sources3
Beta strandi116 – 119Combined sources4
Helixi161 – 172Combined sources12
Helixi177 – 185Combined sources9
Turni186 – 188Combined sources3
Helixi192 – 200Combined sources9
Helixi233 – 237Combined sources5
Helixi239 – 246Combined sources8
Turni248 – 250Combined sources3
Helixi254 – 259Combined sources6
Turni260 – 265Combined sources6
Helixi267 – 285Combined sources19
Beta strandi298 – 301Combined sources4
Turni318 – 320Combined sources3
Helixi321 – 324Combined sources4
Turni325 – 331Combined sources7
Helixi334 – 341Combined sources8
Turni342 – 345Combined sources4
Helixi348 – 354Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DV0NMR-A319-363[»]
1F4INMR-A319-363[»]
1IFYNMR-A156-204[»]
1OQYNMR-A1-363[»]
1P98NMR-A1-78[»]
1P9DNMR-U1-78[»]
1QZENMR-A2-363[»]
1TP4NMR-A223-317[»]
1ZO6model-A315-363[»]
2WYQX-ray1.65A1-82[»]
DisProtiDP00156.
ProteinModelPortaliP54725.
SMRiP54725.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54725.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 81Ubiquitin-likePROSITE-ProRule annotationAdd BLAST81
Domaini161 – 201UBA 1PROSITE-ProRule annotationAdd BLAST41
Domaini318 – 358UBA 2PROSITE-ProRule annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni319 – 363HIV-1 vpr bindingAdd BLAST45

Domaini

The ubiquitin-like domain mediates interaction with ATXN3.
The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.

Sequence similaritiesi

Belongs to the RAD23 family.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0011. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00390000012078.
HOGENOMiHOG000172162.
HOVERGENiHBG055042.
InParanoidiP54725.
KOiK10839.
OMAiVACCVRI.
OrthoDBiEOG091G0DVL.
PhylomeDBiP54725.

Family and domain databases

Gene3Di1.10.10.540. 1 hit.
InterProiIPR004806. Rad23.
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR015360. XPC-bd.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00240. ubiquitin. 1 hit.
PF09280. XPC-binding. 1 hit.
[Graphical view]
PRINTSiPR01839. RAD23PROTEIN.
SMARTiSM00727. STI1. 1 hit.
SM00165. UBA. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF101238. SSF101238. 1 hit.
SSF46934. SSF46934. 2 hits.
SSF54236. SSF54236. 1 hit.
TIGRFAMsiTIGR00601. rad23. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P54725-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVTITLKTL QQQTFKIRME PDETVKVLKE KIEAEKGRDA FPVAGQKLIY
60 70 80 90 100
AGKILSDDVP IRDYRIDEKN FVVVMVTKTK AGQGTSAPPE ASPTAAPESS
110 120 130 140 150
TSFPPAPTSG MSHPPPAARE DKSPSEESAP TTSPESVSGS VPSSGSSGRE
160 170 180 190 200
EDAASTLVTG SEYETMLTEI MSMGYERERV VAALRASYNN PHRAVEYLLT
210 220 230 240 250
GIPGSPEPEH GSVQESQVSE QPATEAAGEN PLEFLRDQPQ FQNMRQVIQQ
260 270 280 290 300
NPALLPALLQ QLGQENPQLL QQISRHQEQF IQMLNEPPGE LADISDVEGE
310 320 330 340 350
VGAIGEEAPQ MNYIQVTPQE KEAIERLKAL GFPESLVIQA YFACEKNENL
360
AANFLLSQNF DDE
Length:363
Mass (Da):39,609
Last modified:October 1, 1996 - v1
Checksum:iC4E47E9313BB47B5
GO
Isoform 2 (identifier: P54725-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     272-326: Missing.

Note: No experimental confirmation available.
Show »
Length:308
Mass (Da):33,433
Checksum:iE09AD30E925EEBAD
GO
Isoform 3 (identifier: P54725-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     226-226: Missing.

Note: No experimental confirmation available.
Show »
Length:362
Mass (Da):39,538
Checksum:iE6A8DC2ADC3813F6
GO

Sequence cautioni

The sequence BAD92950 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BX448989 differs from that shown. Reason: Frameshift at positions 159, 333, 339 and 347.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_016251131T → A.2 PublicationsCorresponds to variant rs11558955dbSNPEnsembl.1
Natural variantiVAR_020377179R → Q.Corresponds to variant rs4987203dbSNPEnsembl.1
Natural variantiVAR_016252200T → M.1 PublicationCorresponds to variant rs4987202dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054694226Missing in isoform 3. 2 Publications1
Alternative sequenceiVSP_047565272 – 326Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21235 mRNA. Translation: BAA04767.1.
AF549209 Genomic DNA. Translation: AAN39383.1.
BX448989 mRNA. No translation available.
AB209713 mRNA. Translation: BAD92950.1. Different initiation.
AC092069 Genomic DNA. No translation available.
AD000092 Genomic DNA. Translation: AAB51177.1.
BC014026 mRNA. Translation: AAH14026.1.
BC088364 mRNA. Translation: AAH88364.1.
CCDSiCCDS12289.1. [P54725-1]
CCDS59357.1. [P54725-2]
CCDS59358.1. [P54725-3]
PIRiS44443.
RefSeqiNP_001257291.1. NM_001270362.1. [P54725-3]
NP_001257292.1. NM_001270363.1. [P54725-2]
NP_005044.1. NM_005053.3. [P54725-1]
UniGeneiHs.643267.

Genome annotation databases

EnsembliENST00000316856; ENSP00000321365; ENSG00000179262. [P54725-3]
ENST00000586534; ENSP00000467024; ENSG00000179262. [P54725-1]
ENST00000592268; ENSP00000468674; ENSG00000179262. [P54725-2]
GeneIDi5886.
KEGGihsa:5886.
UCSCiuc002mvw.3. human. [P54725-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21235 mRNA. Translation: BAA04767.1.
AF549209 Genomic DNA. Translation: AAN39383.1.
BX448989 mRNA. No translation available.
AB209713 mRNA. Translation: BAD92950.1. Different initiation.
AC092069 Genomic DNA. No translation available.
AD000092 Genomic DNA. Translation: AAB51177.1.
BC014026 mRNA. Translation: AAH14026.1.
BC088364 mRNA. Translation: AAH88364.1.
CCDSiCCDS12289.1. [P54725-1]
CCDS59357.1. [P54725-2]
CCDS59358.1. [P54725-3]
PIRiS44443.
RefSeqiNP_001257291.1. NM_001270362.1. [P54725-3]
NP_001257292.1. NM_001270363.1. [P54725-2]
NP_005044.1. NM_005053.3. [P54725-1]
UniGeneiHs.643267.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DV0NMR-A319-363[»]
1F4INMR-A319-363[»]
1IFYNMR-A156-204[»]
1OQYNMR-A1-363[»]
1P98NMR-A1-78[»]
1P9DNMR-U1-78[»]
1QZENMR-A2-363[»]
1TP4NMR-A223-317[»]
1ZO6model-A315-363[»]
2WYQX-ray1.65A1-82[»]
DisProtiDP00156.
ProteinModelPortaliP54725.
SMRiP54725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111823. 241 interactors.
DIPiDIP-34442N.
IntActiP54725. 60 interactors.
MINTiMINT-105454.
STRINGi9606.ENSP00000467024.

PTM databases

iPTMnetiP54725.
PhosphoSitePlusiP54725.

Polymorphism and mutation databases

BioMutaiRAD23A.
DMDMi1709983.

Proteomic databases

EPDiP54725.
MaxQBiP54725.
PaxDbiP54725.
PeptideAtlasiP54725.
PRIDEiP54725.

Protocols and materials databases

DNASUi5886.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316856; ENSP00000321365; ENSG00000179262. [P54725-3]
ENST00000586534; ENSP00000467024; ENSG00000179262. [P54725-1]
ENST00000592268; ENSP00000468674; ENSG00000179262. [P54725-2]
GeneIDi5886.
KEGGihsa:5886.
UCSCiuc002mvw.3. human. [P54725-1]

Organism-specific databases

CTDi5886.
DisGeNETi5886.
GeneCardsiRAD23A.
HGNCiHGNC:9812. RAD23A.
HPAiHPA065599.
MIMi600061. gene.
neXtProtiNX_P54725.
OpenTargetsiENSG00000179262.
PharmGKBiPA34172.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0011. Eukaryota.
COG5272. LUCA.
GeneTreeiENSGT00390000012078.
HOGENOMiHOG000172162.
HOVERGENiHBG055042.
InParanoidiP54725.
KOiK10839.
OMAiVACCVRI.
OrthoDBiEOG091G0DVL.
PhylomeDBiP54725.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000179262-MONOMER.
ReactomeiR-HSA-5689877. Josephin domain DUBs.
R-HSA-5696394. DNA Damage Recognition in GG-NER.
R-HSA-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

ChiTaRSiRAD23A. human.
EvolutionaryTraceiP54725.
GeneWikiiRAD23A.
GenomeRNAii5886.
PMAP-CutDBP54725.
PROiP54725.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000179262.
CleanExiHS_RAD23A.
ExpressionAtlasiP54725. baseline and differential.
GenevisibleiP54725. HS.

Family and domain databases

Gene3Di1.10.10.540. 1 hit.
InterProiIPR004806. Rad23.
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR015360. XPC-bd.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00240. ubiquitin. 1 hit.
PF09280. XPC-binding. 1 hit.
[Graphical view]
PRINTSiPR01839. RAD23PROTEIN.
SMARTiSM00727. STI1. 1 hit.
SM00165. UBA. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF101238. SSF101238. 1 hit.
SSF46934. SSF46934. 2 hits.
SSF54236. SSF54236. 1 hit.
TIGRFAMsiTIGR00601. rad23. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRD23A_HUMAN
AccessioniPrimary (citable) accession number: P54725
Secondary accession number(s): K7ESE3, Q59EU8, Q5M7Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 174 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.