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Protein

Catechol-2,3-dioxygenase

Gene

catE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the meta cleavage of catechol to 2-hydroxymuconic semialdehyde. Essential for growth and viability in the presence of catechol and probably involved in the detoxification of catechol.1 Publication

Catalytic activityi

Catechol + O2 = 2-hydroxymuconate-6-semialdehyde.1 Publication

Cofactori

Fe2+By similarity

Kineticsi

    1. Vmax=1.04 µmol/min/mg enzyme (at pH 7 and at 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi213IronBy similarity1
    Metal bindingi264IronBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionDioxygenase, Oxidoreductase
    Biological processAromatic hydrocarbons catabolism, Detoxification
    LigandIron, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU08240-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catechol-2,3-dioxygenase (EC:1.13.11.2)
    Alternative name(s):
    Catechol-induced ring cleavage extradiol dioxygenase
    Gene namesi
    Name:catE
    Synonyms:yfiE
    Ordered Locus Names:BSU08240
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000495251 – 285Catechol-2,3-dioxygenaseAdd BLAST285

    Proteomic databases

    PaxDbiP54721
    PRIDEiP54721

    Expressioni

    Inductioni

    Strongly induced by catechol, less strongly by 2-methylhydroquinone (2-MHQ) but only weakly by chromanon (6-brom-2-vinyl-chroman-4-on).2 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100004578

    Structurei

    3D structure databases

    ProteinModelPortaliP54721
    SMRiP54721
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini9 – 126VOC 1PROSITE-ProRule annotationAdd BLAST118
    Domaini169 – 285VOC 2PROSITE-ProRule annotationAdd BLAST117

    Sequence similaritiesi

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiENOG4108TKW Bacteria
    COG2514 LUCA
    HOGENOMiHOG000250737
    InParanoidiP54721
    KOiK07104
    OMAiGGYHHHM
    PhylomeDBiP54721

    Family and domain databases

    Gene3Di3.10.180.10, 2 hits
    InterProiView protein in InterPro
    IPR029068 Glyas_Bleomycin-R_OHBP_Dase
    IPR004360 Glyas_Fos-R_dOase_dom
    IPR037523 VOC
    IPR000486 Xdiol_ring_cleave_dOase_1/2
    PfamiView protein in Pfam
    PF00903 Glyoxalase, 2 hits
    SUPFAMiSSF54593 SSF54593, 2 hits
    PROSITEiView protein in PROSITE
    PS00082 EXTRADIOL_DIOXYGENAS, 1 hit
    PS51819 VOC, 2 hits

    Sequencei

    Sequence statusi: Complete.

    P54721-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTSIHEDTHI GYAKLTIRSL ERSLQFYCNV IGFQVLKKTD RQAELTADGK
    60 70 80 90 100
    RVLLILEENP SAVVLPERSV TGLYHFAILL PDRKELGIAL ARLIEHGIAI
    110 120 130 140 150
    GHGDHAVSEA LYLSDPDGNG IEMYADRPRS TWQRDREGNY VMTTTAVDIE
    160 170 180 190 200
    GLLEEAGDER KTSLPNDTII GHIHLHVSDL KEAKAFYTDV LGFDIVGNYA
    210 220 230 240 250
    GMSALFVSAG GYHHHIGLNI WAGRNAPPKP TNASGLDYYT VVLPHQEELD
    260 270 280
    LVANRVKHAG YSIEETENSF RVKDPVSGAY ITFVI
    Length:285
    Mass (Da):31,565
    Last modified:June 16, 2009 - v2
    Checksum:iCCDA3E510D89C9FC
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti68R → L in BAA09109 (PubMed:8704981).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D50543 Genomic DNA Translation: BAA09109.1
    AL009126 Genomic DNA Translation: CAB12653.2
    PIRiH69802
    RefSeqiNP_388705.2, NC_000964.3
    WP_003233597.1, NZ_JNCM01000032.1

    Genome annotation databases

    EnsemblBacteriaiCAB12653; CAB12653; BSU08240
    GeneIDi936164
    KEGGibsu:BSU08240
    PATRICifig|224308.179.peg.890

    Similar proteinsi

    Entry informationi

    Entry nameiCADE_BACSU
    AccessioniPrimary (citable) accession number: P54721
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: June 16, 2009
    Last modified: May 23, 2018
    This is version 103 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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