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P54721 (CADE_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Catechol-2,3-dioxygenase

EC=1.13.11.2
Alternative name(s):
Catechol-induced ring cleavage extradiol dioxygenase
Gene names
Name:catE
Synonyms:yfiE
Ordered Locus Names:BSU08240
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the meta cleavage of catechol to 2-hydroxymuconic semialdehyde. Essential for growth and viability in the presence of catechol and probably involved in the detoxification of catechol. Ref.4

Catalytic activity

Catechol + O2 = 2-hydroxymuconate-6-semialdehyde. Ref.4

Cofactor

Fe2+ ion By similarity.

Induction

Strongly induced by catechol, less strongly by 2-methylhydroquinone (2-MHQ) but only weakly by chromanon (6-brom-2-vinyl-chroman-4-on). Ref.4 Ref.5

Sequence similarities

Belongs to the extradiol ring-cleavage dioxygenase family.

Biophysicochemical properties

Kinetic parameters:

Vmax=1.04 µmol/min/mg enzyme (at pH 7 and at 25 degrees Celsius) Ref.4

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Catechol-2,3-dioxygenase
PRO_0000049525

Sites

Metal binding2131Iron By similarity
Metal binding2641Iron By similarity

Experimental info

Sequence conflict681R → L in BAA09109. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54721 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: CCDA3E510D89C9FC

FASTA28531,565
        10         20         30         40         50         60 
MTSIHEDTHI GYAKLTIRSL ERSLQFYCNV IGFQVLKKTD RQAELTADGK RVLLILEENP 

        70         80         90        100        110        120 
SAVVLPERSV TGLYHFAILL PDRKELGIAL ARLIEHGIAI GHGDHAVSEA LYLSDPDGNG 

       130        140        150        160        170        180 
IEMYADRPRS TWQRDREGNY VMTTTAVDIE GLLEEAGDER KTSLPNDTII GHIHLHVSDL 

       190        200        210        220        230        240 
KEAKAFYTDV LGFDIVGNYA GMSALFVSAG GYHHHIGLNI WAGRNAPPKP TNASGLDYYT 

       250        260        270        280 
VVLPHQEELD LVANRVKHAG YSIEETENSF RVKDPVSGAY ITFVI 

« Hide

References

« Hide 'large scale' references
[1]"Determination of a 12 kb nucleotide sequence around the 76 degrees region of the Bacillus subtilis chromosome."
Yamamoto H., Uchiyama S., Fajar A.N., Ogasawara N., Sekiguchi J.
Microbiology 142:1417-1421(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 68.
[4]"Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis."
Tam le T., Eymann C., Albrecht D., Sietmann R., Schauer F., Hecker M., Antelmann H.
Environ. Microbiol. 8:1408-1427(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
Strain: 168.
[5]"Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis."
Nguyen V.D., Wolf C., Maeder U., Lalk M., Langer P., Lindequist U., Hecker M., Antelmann H.
Proteomics 7:1391-1408(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, NOMENCLATURE.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50543 Genomic DNA. Translation: BAA09109.1.
AL009126 Genomic DNA. Translation: CAB12653.2.
PIRH69802.
RefSeqNP_388705.2. NC_000964.3.

3D structure databases

ProteinModelPortalP54721.
SMRP54721. Positions 6-137, 167-283.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU08240.

Proteomic databases

PaxDbP54721.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12653; CAB12653; BSU08240.
GeneID936164.
KEGGbsu:BSU08240.
PATRIC18973298. VBIBacSub10457_0863.

Organism-specific databases

GenoListBSU08240. [Micado]

Phylogenomic databases

eggNOGCOG2514.
HOGENOMHOG000250737.
OrthoDBEOG6VQPR2.
ProtClustDBCLSK886889.

Enzyme and pathway databases

BioCycBSUB:BSU08240-MONOMER.

Family and domain databases

InterProIPR004360. Glyas_Fos-R_dOase_dom.
IPR000486. Xdiol_ring_cleave_dOase_1/2.
[Graphical view]
PfamPF00903. Glyoxalase. 2 hits.
[Graphical view]
PROSITEPS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCADE_BACSU
AccessionPrimary (citable) accession number: P54721
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 16, 2009
Last modified: November 13, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList