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P54721

- CADE_BACSU

UniProt

P54721 - CADE_BACSU

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Protein
Catechol-2,3-dioxygenase
Gene
catE, yfiE, BSU08240
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the meta cleavage of catechol to 2-hydroxymuconic semialdehyde. Essential for growth and viability in the presence of catechol and probably involved in the detoxification of catechol.1 Publication

Catalytic activityi

Catechol + O2 = 2-hydroxymuconate-6-semialdehyde.1 Publication

Cofactori

Fe2+ ion By similarity.

Kineticsi

    Vmax=1.04 µmol/min/mg enzyme (at pH 7 and at 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi213 – 2131Iron By similarity
    Metal bindingi264 – 2641Iron By similarity

    GO - Molecular functioni

    1. catechol 2,3-dioxygenase activity Source: UniProtKB-EC
    2. ferrous iron binding Source: InterPro

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW
    2. response to toxic substance Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism, Detoxification

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU08240-MONOMER.
    RETL1328306-WGS:GSTH-1235-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catechol-2,3-dioxygenase (EC:1.13.11.2)
    Alternative name(s):
    Catechol-induced ring cleavage extradiol dioxygenase
    Gene namesi
    Name:catE
    Synonyms:yfiE
    Ordered Locus Names:BSU08240
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU08240. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Catechol-2,3-dioxygenase
    PRO_0000049525Add
    BLAST

    Proteomic databases

    PaxDbiP54721.

    Expressioni

    Inductioni

    Strongly induced by catechol, less strongly by 2-methylhydroquinone (2-MHQ) but only weakly by chromanon (6-brom-2-vinyl-chroman-4-on).2 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU08240.

    Structurei

    3D structure databases

    ProteinModelPortaliP54721.
    SMRiP54721. Positions 6-137, 167-283.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2514.
    HOGENOMiHOG000250737.
    OrthoDBiEOG6VQPR2.
    PhylomeDBiP54721.

    Family and domain databases

    Gene3Di3.10.180.10. 2 hits.
    InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR004360. Glyas_Fos-R_dOase_dom.
    IPR000486. Xdiol_ring_cleave_dOase_1/2.
    [Graphical view]
    PfamiPF00903. Glyoxalase. 2 hits.
    [Graphical view]
    SUPFAMiSSF54593. SSF54593. 2 hits.
    PROSITEiPS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54721-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSIHEDTHI GYAKLTIRSL ERSLQFYCNV IGFQVLKKTD RQAELTADGK    50
    RVLLILEENP SAVVLPERSV TGLYHFAILL PDRKELGIAL ARLIEHGIAI 100
    GHGDHAVSEA LYLSDPDGNG IEMYADRPRS TWQRDREGNY VMTTTAVDIE 150
    GLLEEAGDER KTSLPNDTII GHIHLHVSDL KEAKAFYTDV LGFDIVGNYA 200
    GMSALFVSAG GYHHHIGLNI WAGRNAPPKP TNASGLDYYT VVLPHQEELD 250
    LVANRVKHAG YSIEETENSF RVKDPVSGAY ITFVI 285
    Length:285
    Mass (Da):31,565
    Last modified:June 16, 2009 - v2
    Checksum:iCCDA3E510D89C9FC
    GO

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681R → L in BAA09109. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50543 Genomic DNA. Translation: BAA09109.1.
    AL009126 Genomic DNA. Translation: CAB12653.2.
    PIRiH69802.
    RefSeqiNP_388705.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB12653; CAB12653; BSU08240.
    GeneIDi936164.
    KEGGibsu:BSU08240.
    PATRICi18973298. VBIBacSub10457_0863.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50543 Genomic DNA. Translation: BAA09109.1 .
    AL009126 Genomic DNA. Translation: CAB12653.2 .
    PIRi H69802.
    RefSeqi NP_388705.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P54721.
    SMRi P54721. Positions 6-137, 167-283.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU08240.

    Proteomic databases

    PaxDbi P54721.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12653 ; CAB12653 ; BSU08240 .
    GeneIDi 936164.
    KEGGi bsu:BSU08240.
    PATRICi 18973298. VBIBacSub10457_0863.

    Organism-specific databases

    GenoListi BSU08240. [Micado ]

    Phylogenomic databases

    eggNOGi COG2514.
    HOGENOMi HOG000250737.
    OrthoDBi EOG6VQPR2.
    PhylomeDBi P54721.

    Enzyme and pathway databases

    BioCyci BSUB:BSU08240-MONOMER.
    RETL1328306-WGS:GSTH-1235-MONOMER.

    Family and domain databases

    Gene3Di 3.10.180.10. 2 hits.
    InterProi IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR004360. Glyas_Fos-R_dOase_dom.
    IPR000486. Xdiol_ring_cleave_dOase_1/2.
    [Graphical view ]
    Pfami PF00903. Glyoxalase. 2 hits.
    [Graphical view ]
    SUPFAMi SSF54593. SSF54593. 2 hits.
    PROSITEi PS00082. EXTRADIOL_DIOXYGENAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Determination of a 12 kb nucleotide sequence around the 76 degrees region of the Bacillus subtilis chromosome."
      Yamamoto H., Uchiyama S., Fajar A.N., Ogasawara N., Sekiguchi J.
      Microbiology 142:1417-1421(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 68.
    4. "Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis."
      Tam le T., Eymann C., Albrecht D., Sietmann R., Schauer F., Hecker M., Antelmann H.
      Environ. Microbiol. 8:1408-1427(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
      Strain: 168.
    5. "Transcriptome and proteome analyses in response to 2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation systems involved in the catabolism of aromatic compounds in Bacillus subtilis."
      Nguyen V.D., Wolf C., Maeder U., Lalk M., Langer P., Lindequist U., Hecker M., Antelmann H.
      Proteomics 7:1391-1408(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, NOMENCLATURE.
      Strain: 168.

    Entry informationi

    Entry nameiCADE_BACSU
    AccessioniPrimary (citable) accession number: P54721
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: June 16, 2009
    Last modified: July 9, 2014
    This is version 83 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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