P54709 (AT1B3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-3

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-3
Short name=ATPB-3
CD_antigen=CD298

Gene names

Name:

ATP1B3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Cell membrane; Single-pass type II membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.5
Domain	The C-terminal lobe folds into an immunoglobulin-like domain and may mediate cell adhesion properties By similarity.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome ion transmembrane transport Traceable author statement. Source: Reactome leukocyte migration Traceable author statement. Source: Reactome sodium ion transmembrane transport Traceable author statement Ref.1. Source: GOC transmembrane transport Traceable author statement. Source: Reactome transport Traceable author statement Ref.1. Source: ProtInc
Cellular_component	caveola Inferred from electronic annotation. Source: Ensembl extracellular vesicular exosome Inferred from direct assay PubMed 20458337. Source: UniProt melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: Reactome sodium:potassium-exchanging ATPase complex Traceable author statement Ref.1. Source: ProtInc
Molecular_function	sodium:potassium-exchanging ATPase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Sodium/potassium-transporting ATPase subunit beta-3		PRO_0000219108
Regions
	Topological domain	1 – 35	35	Cytoplasmic Potential
	Transmembrane	36 – 56	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	57 – 279	223	Extracellular Potential
	Region	186 – 279	94	immunoglobulin-like By similarity
Amino acid modifications
	Glycosylation	124	1	N-linked (GlcNAc...) Potential
	Glycosylation	240	1	N-linked (GlcNAc...) Ref.4
	Disulfide bond	128 ↔ 144		By similarity
	Disulfide bond	154 ↔ 170		By similarity
	Disulfide bond	191 ↔ 250		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54709 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D92ECB4F0F6EBFE5	FASTA	279	31,513
	10 20 30 40 50 60 MTKNEKKSLN QSLAEWKLFI YNPTTGEFLG RTAKSWGLIL LFYLVFYGFL AALFSFTMWV 70 80 90 100 110 120 MLQTLNDEVP KYRDQIPSPG LMVFPKPVTA LEYTFSRSDP TSYAGYIEDL KKFLKPYTLE 130 140 150 160 170 180 EQKNLTVCPD GALFEQKGPV YVACQFPISL LQACSGMNDP DFGYSQGNPC ILVKMNRIIG 190 200 210 220 230 240 LKPEGVPRID CVSKNEDIPN VAVYPHNGMI DLKYFPYYGK KLHVGYLQPL VAVQVSFAPN 250 260 270 NTGKEVTVEC KIDGSANLKS QDDRDKFLGR VMFKITARA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the mammalian Na,K-ATPase 3 subunit." Malik N., Canfield V.A., Beckers M.C., Gros P., Levenson R. J. Biol. Chem. 271:22754-22758(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Structural organization and chromosomal localization of the human Na,K- ATPase beta 3 subunit gene and pseudogene." Malik N., Canfield V., Sanchez-Watts G., Watts A.G., Scherer S., Beatty B.G., Gros P., Levenson R. Mamm. Genome 9:136-143(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-240.
[5]	"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F. J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Tissue: Melanoma.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51478 mRNA. Translation: AAC50665.1. AF005896 , AF005890, AF005891, AF005892, AF005893, AF005894, AF005895 Genomic DNA. Translation: AAB61713.1. BC011835 mRNA. Translation: AAH11835.1.
PIR	G02485.
RefSeq	NP_001670.1. NM_001679.2.
UniGene	Hs.477789.
3D structure databases
ProteinModelPortal	P54709.
SMR	P54709. Positions 1-279.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	106973. 8 interactions.
IntAct	P54709. 4 interactions.
MINT	MINT-5001083.
STRING	9606.ENSP00000286371.
Chemistry
BindingDB	P54709.
ChEMBL	CHEMBL2095186.
Protein family/group databases
TCDB	3.A.3.1.1. the p-type atpase (p-atpase) superfamily.
PTM databases
PhosphoSite	P54709.
Polymorphism databases
DMDM	1703470.
Proteomic databases
PaxDb	P54709.
PRIDE	P54709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000286371; ENSP00000286371; ENSG00000069849.
GeneID	483.
KEGG	hsa:483.
UCSC	uc003eug.1. human.
Organism-specific databases
CTD	483.
GeneCards	GC03P141594.
HGNC	HGNC:806. ATP1B3.
HPA	CAB020697.
MIM	601867. gene.
neXtProt	NX_P54709.
PharmGKB	PA68.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG263581.
HOGENOM	HOG000039248.
HOVERGEN	HBG050603.
InParanoid	P54709.
KO	K01540.
OMA	YDLEEQK.
PhylomeDB	P54709.
TreeFam	TF314618.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P54709.
Bgee	P54709.
CleanEx	HS_ATP1B3.
Genevestigator	P54709.
Family and domain databases
InterPro	IPR000402. Na/K_ATPase_sub_beta. [Graphical view]
PANTHER	PTHR11523. PTHR11523. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GeneWiki	ATP1B3.
GenomeRNAi	483.
NextBio	2005.
PRO	P54709.
SOURCE	Search...

Entry information

Entry name

AT1B3_HUMAN

Accession

Primary (citable) accession number: P54709

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 16, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.