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Protein

Potassium-transporting ATPase alpha chain 2

Gene

Atp12a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3884-aspartylphosphate intermediateBy similarity1
Metal bindingi729MagnesiumBy similarity1
Metal bindingi733MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: GO_Central
  • cellular potassium ion homeostasis Source: GO_Central
  • cellular sodium ion homeostasis Source: GO_Central
  • metal ion transport Source: RGD
  • potassium ion import Source: GO_Central
  • potassium ion transport Source: RGD
  • response to metal ion Source: RGD
  • response to organic cyclic compound Source: RGD
  • sodium ion export from cell Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Protein family/group databases

TCDBi3.A.3.1.4. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp12a
Synonyms:Atp1al1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620569. Atp12a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 99CytoplasmicSequence analysisAdd BLAST98
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121 – 143LumenalSequence analysisAdd BLAST23
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 300CytoplasmicSequence analysisAdd BLAST136
Transmembranei301 – 320HelicalSequence analysisAdd BLAST20
Topological domaini321 – 332LumenalSequence analysisAdd BLAST12
Transmembranei333 – 350HelicalSequence analysisAdd BLAST18
Topological domaini351 – 784CytoplasmicSequence analysisAdd BLAST434
Transmembranei785 – 804HelicalSequence analysisAdd BLAST20
Topological domaini805 – 814LumenalSequence analysis10
Transmembranei815 – 835HelicalSequence analysisAdd BLAST21
Topological domaini836 – 855CytoplasmicSequence analysisAdd BLAST20
Transmembranei856 – 878HelicalSequence analysisAdd BLAST23
Topological domaini879 – 930LumenalSequence analysisAdd BLAST52
Transmembranei931 – 950HelicalSequence analysisAdd BLAST20
Topological domaini951 – 964CytoplasmicSequence analysisAdd BLAST14
Transmembranei965 – 983HelicalSequence analysisAdd BLAST19
Topological domaini984 – 998LumenalSequence analysisAdd BLAST15
Transmembranei999 – 1019HelicalSequence analysisAdd BLAST21
Topological domaini1020 – 1036CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000462632 – 1036Potassium-transporting ATPase alpha chain 2Add BLAST1035

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphotyrosineBy similarity1
Modified residuei9PhosphoserineBy similarity1
Modified residuei60PhosphoserineBy similarity1
Modified residuei240PhosphoserineBy similarity1
Modified residuei287PhosphoserineBy similarity1
Modified residuei464PhosphoserineBy similarity1
Modified residuei602PhosphoserineBy similarity1
Modified residuei841PhosphoserineBy similarity1
Modified residuei955Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54708.
PRIDEiP54708.

PTM databases

iPTMnetiP54708.
PhosphoSitePlusiP54708.

Expressioni

Tissue specificityi

Expressed at high levels in distal colon, coagulating and preputial glands; at much lower levels in proximal colon, kidney, uterus, brain, placenta and lung; and at trace levels in heart and forestomach.1 Publication

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028093.

Structurei

3D structure databases

ProteinModelPortaliP54708.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP54708.
PhylomeDBiP54708.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 2 hits.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P54708-1) [UniParc]FASTAAdd to basket
Also known as: 2a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRKTEIYSV ELNGTKDVKP ADQRDDKKFK GAKNKDLEPN KSHEKEELKK
60 70 80 90 100
ELDLDDHRLS NTELEQKYGT NIIQGLSSVR ATELLARDGP NTLTPPKQTP
110 120 130 140 150
EIIKFLKQMV GGFSILLWIG AALCWIAFVI QYVNNSASLD NVYLGAILVL
160 170 180 190 200
VVILTGIFAY YQEAKSTNIM ASFSKMIPQQ ALVIRDAEKK VISAEQLVVG
210 220 230 240 250
DVVEIKGGDQ IPADIRLVFS QGCKVDNSSL TGESEPQARS TEFTHENPLE
260 270 280 290 300
TKNIGFYSTT CLEGTATGIV INTGDRTIIG RIASLASGVG SEKTPIAIEI
310 320 330 340 350
EHFVHIVAGV AVSIDIIFFI TAVCMKYYVL DAIIFLISII VANVPEGLLA
360 370 380 390 400
TVTVTLSLTA KRMAKKNCLV KNLEAVETLG STSIICSDKT GTLTQNRMTV
410 420 430 440 450
AHLWFDNQIF VADTSENQTK QAFDQSSGTW ASLSKIITLC NRAEFRPGQE
460 470 480 490 500
SVPIMKRTVV GDASETALLK FSEVILGDVM GIRKRNHKVA EIPFNSTNKF
510 520 530 540 550
QLSIHETEDP NNKRFLVVMK GAPERILEKC STIMINGQEQ PLDKSSADSF
560 570 580 590 600
HTAYMELGGL GERVLGFCHL YLPAEQFPQS YIFDVDSVNF PTSNFCFVGL
610 620 630 640 650
LSMIDPPRST VPDAVSKCRS AGIKVIMVTG DHPITAKAIA KSVGIISANN
660 670 680 690 700
ETVEDIAKRR NIAVEQVNKR EAKAAVVTGM ELKDMTPEQL DELLTNYQEI
710 720 730 740 750
VFARTSPQQK LIIVEGCQRQ DAIVAVTGDG VNDSPALKKA DIGIAMGIAG
760 770 780 790 800
SDAAKNAADM VLLDDNFASI VTGVEEGRLI FDNLKKTIAY TLTKNIAELC
810 820 830 840 850
PFLIYIVAGL PLPIGTITIL FIDLGTDIIP SIALAYEKAE SDIMNRKPRH
860 870 880 890 900
KKKDRLVNTQ LAIYSYLHIG LMQALGGFLV YFTVYAQQGF WPTSLINLRV
910 920 930 940 950
AWETDDINDL EDSYGQEWTR YQRKYLEWTG STAFFVAIMI QQIADLIIRK
960 970 980 990 1000
TRRNSIFQQG LFRNKVIWVG IASQVIVALI LSYGLGSVPA LSFTMLRVQY
1010 1020 1030
WFVAVPHAIL IWVYDEMRKL FIRLYPGSWW DKNMYY
Length:1,036
Mass (Da):114,975
Last modified:October 1, 1996 - v1
Checksum:i453918E65CD4813B
GO
Isoform Short (identifier: P54708-2) [UniParc]FASTAAdd to basket
Also known as: 2b

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Show »
Length:928
Mass (Da):102,562
Checksum:iEB586EC235C4CB85
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0004141 – 108Missing in isoform Short. 1 PublicationAdd BLAST108

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90398 mRNA. Translation: AAA40779.1.
U94911 Genomic DNA. Translation: AAB93900.1.
U94912 mRNA. Translation: AAB93901.1.
U94913 mRNA. Translation: AAB93902.1.
PIRiA42895.
UniGeneiRn.9858.

Genome annotation databases

UCSCiRGD:620569. rat. [P54708-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90398 mRNA. Translation: AAA40779.1.
U94911 Genomic DNA. Translation: AAB93900.1.
U94912 mRNA. Translation: AAB93901.1.
U94913 mRNA. Translation: AAB93902.1.
PIRiA42895.
UniGeneiRn.9858.

3D structure databases

ProteinModelPortaliP54708.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028093.

Protein family/group databases

TCDBi3.A.3.1.4. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiP54708.
PhosphoSitePlusiP54708.

Proteomic databases

PaxDbiP54708.
PRIDEiP54708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620569. rat. [P54708-1]

Organism-specific databases

RGDi620569. Atp12a.

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP54708.
PhylomeDBiP54708.

Miscellaneous databases

PROiP54708.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 2 hits.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT12A_RAT
AccessioniPrimary (citable) accession number: P54708
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.