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P54708

- AT12A_RAT

UniProt

P54708 - AT12A_RAT

Protein

Potassium-transporting ATPase alpha chain 2

Gene

Atp12a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

    Catalytic activityi

    ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei388 – 38814-aspartylphosphate intermediateBy similarity
    Metal bindingi729 – 7291MagnesiumBy similarity
    Metal bindingi733 – 7331MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrogen:potassium-exchanging ATPase activity Source: RGD
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro
    2. hydrogen ion transmembrane transport Source: GOC
    3. metal ion transport Source: RGD
    4. potassium ion transport Source: RGD
    5. response to metal ion Source: RGD
    6. response to organic cyclic compound Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Hydrogen ion transport, Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

    Protein family/group databases

    TCDBi3.A.3.1.4. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
    Alternative name(s):
    Non-gastric H(+)/K(+) ATPase subunit alpha
    Proton pump
    Gene namesi
    Name:Atp12a
    Synonyms:Atp1al1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620569. Atp12a.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10361036Potassium-transporting ATPase alpha chain 2PRO_0000046263Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei955 – 9551Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP54708.
    PRIDEiP54708.

    PTM databases

    PhosphoSiteiP54708.

    Expressioni

    Tissue specificityi

    Expressed at high levels in distal colon, coagulating and preputial glands; at much lower levels in proximal colon, kidney, uterus, brain, placenta and lung; and at trace levels in heart and forestomach.1 Publication

    Gene expression databases

    GenevestigatoriP54708.

    Interactioni

    Subunit structurei

    Composed of two subunits: alpha (catalytic) and beta.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028093.

    Structurei

    3D structure databases

    ProteinModelPortaliP54708.
    SMRiP54708. Positions 41-1036.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9999CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini121 – 14323LumenalSequence AnalysisAdd
    BLAST
    Topological domaini165 – 300136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini321 – 33212LumenalSequence AnalysisAdd
    BLAST
    Topological domaini351 – 784434CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini805 – 81410LumenalSequence Analysis
    Topological domaini836 – 85520CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini879 – 93052LumenalSequence AnalysisAdd
    BLAST
    Topological domaini951 – 96414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini984 – 99815LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1020 – 103617CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei100 – 12021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei301 – 32020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei333 – 35018HelicalSequence AnalysisAdd
    BLAST
    Transmembranei785 – 80420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei815 – 83521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei856 – 87823HelicalSequence AnalysisAdd
    BLAST
    Transmembranei931 – 95020HelicalSequence AnalysisAdd
    BLAST
    Transmembranei965 – 98319HelicalSequence AnalysisAdd
    BLAST
    Transmembranei999 – 101921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    HOGENOMiHOG000265622.
    HOVERGENiHBG004298.
    InParanoidiP54708.
    KOiK01544.
    PhylomeDBiP54708.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P54708-1) [UniParc]FASTAAdd to Basket

    Also known as: 2a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRKTEIYSV ELNGTKDVKP ADQRDDKKFK GAKNKDLEPN KSHEKEELKK     50
    ELDLDDHRLS NTELEQKYGT NIIQGLSSVR ATELLARDGP NTLTPPKQTP 100
    EIIKFLKQMV GGFSILLWIG AALCWIAFVI QYVNNSASLD NVYLGAILVL 150
    VVILTGIFAY YQEAKSTNIM ASFSKMIPQQ ALVIRDAEKK VISAEQLVVG 200
    DVVEIKGGDQ IPADIRLVFS QGCKVDNSSL TGESEPQARS TEFTHENPLE 250
    TKNIGFYSTT CLEGTATGIV INTGDRTIIG RIASLASGVG SEKTPIAIEI 300
    EHFVHIVAGV AVSIDIIFFI TAVCMKYYVL DAIIFLISII VANVPEGLLA 350
    TVTVTLSLTA KRMAKKNCLV KNLEAVETLG STSIICSDKT GTLTQNRMTV 400
    AHLWFDNQIF VADTSENQTK QAFDQSSGTW ASLSKIITLC NRAEFRPGQE 450
    SVPIMKRTVV GDASETALLK FSEVILGDVM GIRKRNHKVA EIPFNSTNKF 500
    QLSIHETEDP NNKRFLVVMK GAPERILEKC STIMINGQEQ PLDKSSADSF 550
    HTAYMELGGL GERVLGFCHL YLPAEQFPQS YIFDVDSVNF PTSNFCFVGL 600
    LSMIDPPRST VPDAVSKCRS AGIKVIMVTG DHPITAKAIA KSVGIISANN 650
    ETVEDIAKRR NIAVEQVNKR EAKAAVVTGM ELKDMTPEQL DELLTNYQEI 700
    VFARTSPQQK LIIVEGCQRQ DAIVAVTGDG VNDSPALKKA DIGIAMGIAG 750
    SDAAKNAADM VLLDDNFASI VTGVEEGRLI FDNLKKTIAY TLTKNIAELC 800
    PFLIYIVAGL PLPIGTITIL FIDLGTDIIP SIALAYEKAE SDIMNRKPRH 850
    KKKDRLVNTQ LAIYSYLHIG LMQALGGFLV YFTVYAQQGF WPTSLINLRV 900
    AWETDDINDL EDSYGQEWTR YQRKYLEWTG STAFFVAIMI QQIADLIIRK 950
    TRRNSIFQQG LFRNKVIWVG IASQVIVALI LSYGLGSVPA LSFTMLRVQY 1000
    WFVAVPHAIL IWVYDEMRKL FIRLYPGSWW DKNMYY 1036
    Length:1,036
    Mass (Da):114,975
    Last modified:October 1, 1996 - v1
    Checksum:i453918E65CD4813B
    GO
    Isoform Short (identifier: P54708-2) [UniParc]FASTAAdd to Basket

    Also known as: 2b

    The sequence of this isoform differs from the canonical sequence as follows:
         1-108: Missing.

    Show »
    Length:928
    Mass (Da):102,562
    Checksum:iEB586EC235C4CB85
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 108108Missing in isoform Short. 1 PublicationVSP_000414Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90398 mRNA. Translation: AAA40779.1.
    U94911 Genomic DNA. Translation: AAB93900.1.
    U94912 mRNA. Translation: AAB93901.1.
    U94913 mRNA. Translation: AAB93902.1.
    PIRiA42895.
    RefSeqiNP_598201.1. NM_133517.1. [P54708-1]
    UniGeneiRn.9858.

    Genome annotation databases

    GeneIDi171028.
    KEGGirno:171028.
    UCSCiRGD:620569. rat. [P54708-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M90398 mRNA. Translation: AAA40779.1 .
    U94911 Genomic DNA. Translation: AAB93900.1 .
    U94912 mRNA. Translation: AAB93901.1 .
    U94913 mRNA. Translation: AAB93902.1 .
    PIRi A42895.
    RefSeqi NP_598201.1. NM_133517.1. [P54708-1 ]
    UniGenei Rn.9858.

    3D structure databases

    ProteinModelPortali P54708.
    SMRi P54708. Positions 41-1036.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000028093.

    Protein family/group databases

    TCDBi 3.A.3.1.4. the p-type atpase (p-atpase) superfamily.

    PTM databases

    PhosphoSitei P54708.

    Proteomic databases

    PaxDbi P54708.
    PRIDEi P54708.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 171028.
    KEGGi rno:171028.
    UCSCi RGD:620569. rat. [P54708-1 ]

    Organism-specific databases

    CTDi 479.
    RGDi 620569. Atp12a.

    Phylogenomic databases

    eggNOGi COG0474.
    HOGENOMi HOG000265622.
    HOVERGENi HBG004298.
    InParanoidi P54708.
    KOi K01544.
    PhylomeDBi P54708.

    Miscellaneous databases

    NextBioi 621525.

    Gene expression databases

    Genevestigatori P54708.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a cDNA encoding the putative distal colon H+,K(+)-ATPase. Similarity of deduced amino acid sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA expression in distal colon, kidney, and uterus."
      Crowson M.S., Shull G.E.
      J. Biol. Chem. 267:13740-13748(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
      Strain: Sprague-Dawley.
      Tissue: Colon.
    2. "A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2 subunit. Cloning, functional expression, and renal adaptive response to chronic hypokalemia."
      Kone B.C., Higham S.C.
      J. Biol. Chem. 273:2543-2552(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS LONG AND SHORT).
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    3. Lubec G., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 779-785, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    4. "Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit."
      Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N.
      FEBS Lett. 440:320-324(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiAT12A_RAT
    AccessioniPrimary (citable) accession number: P54708
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3