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Protein

Potassium-transporting ATPase alpha chain 2

Gene

Atp12a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei388 – 38814-aspartylphosphate intermediateBy similarity
Metal bindingi729 – 7291MagnesiumBy similarity
Metal bindingi733 – 7331MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • hydrogen ion transmembrane transport Source: GOC
  • metal ion transport Source: RGD
  • potassium ion transport Source: RGD
  • response to metal ion Source: RGD
  • response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Protein family/group databases

TCDBi3.A.3.1.4. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp12a
Synonyms:Atp1al1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620569. Atp12a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9999CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence AnalysisAdd
BLAST
Topological domaini121 – 14323LumenalSequence AnalysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence AnalysisAdd
BLAST
Topological domaini165 – 300136CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei301 – 32020HelicalSequence AnalysisAdd
BLAST
Topological domaini321 – 33212LumenalSequence AnalysisAdd
BLAST
Transmembranei333 – 35018HelicalSequence AnalysisAdd
BLAST
Topological domaini351 – 784434CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei785 – 80420HelicalSequence AnalysisAdd
BLAST
Topological domaini805 – 81410LumenalSequence Analysis
Transmembranei815 – 83521HelicalSequence AnalysisAdd
BLAST
Topological domaini836 – 85520CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei856 – 87823HelicalSequence AnalysisAdd
BLAST
Topological domaini879 – 93052LumenalSequence AnalysisAdd
BLAST
Transmembranei931 – 95020HelicalSequence AnalysisAdd
BLAST
Topological domaini951 – 96414CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei965 – 98319HelicalSequence AnalysisAdd
BLAST
Topological domaini984 – 99815LumenalSequence AnalysisAdd
BLAST
Transmembranei999 – 101921HelicalSequence AnalysisAdd
BLAST
Topological domaini1020 – 103617CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Potassium-transporting ATPase alpha chain 2PRO_0000046263Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei955 – 9551Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54708.
PRIDEiP54708.

PTM databases

PhosphoSiteiP54708.

Expressioni

Tissue specificityi

Expressed at high levels in distal colon, coagulating and preputial glands; at much lower levels in proximal colon, kidney, uterus, brain, placenta and lung; and at trace levels in heart and forestomach.1 Publication

Gene expression databases

GenevisibleiP54708. RN.

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028093.

Structurei

3D structure databases

ProteinModelPortaliP54708.
SMRiP54708. Positions 41-1036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP54708.
PhylomeDBiP54708.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 1 hit.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P54708-1) [UniParc]FASTAAdd to basket

Also known as: 2a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRKTEIYSV ELNGTKDVKP ADQRDDKKFK GAKNKDLEPN KSHEKEELKK
60 70 80 90 100
ELDLDDHRLS NTELEQKYGT NIIQGLSSVR ATELLARDGP NTLTPPKQTP
110 120 130 140 150
EIIKFLKQMV GGFSILLWIG AALCWIAFVI QYVNNSASLD NVYLGAILVL
160 170 180 190 200
VVILTGIFAY YQEAKSTNIM ASFSKMIPQQ ALVIRDAEKK VISAEQLVVG
210 220 230 240 250
DVVEIKGGDQ IPADIRLVFS QGCKVDNSSL TGESEPQARS TEFTHENPLE
260 270 280 290 300
TKNIGFYSTT CLEGTATGIV INTGDRTIIG RIASLASGVG SEKTPIAIEI
310 320 330 340 350
EHFVHIVAGV AVSIDIIFFI TAVCMKYYVL DAIIFLISII VANVPEGLLA
360 370 380 390 400
TVTVTLSLTA KRMAKKNCLV KNLEAVETLG STSIICSDKT GTLTQNRMTV
410 420 430 440 450
AHLWFDNQIF VADTSENQTK QAFDQSSGTW ASLSKIITLC NRAEFRPGQE
460 470 480 490 500
SVPIMKRTVV GDASETALLK FSEVILGDVM GIRKRNHKVA EIPFNSTNKF
510 520 530 540 550
QLSIHETEDP NNKRFLVVMK GAPERILEKC STIMINGQEQ PLDKSSADSF
560 570 580 590 600
HTAYMELGGL GERVLGFCHL YLPAEQFPQS YIFDVDSVNF PTSNFCFVGL
610 620 630 640 650
LSMIDPPRST VPDAVSKCRS AGIKVIMVTG DHPITAKAIA KSVGIISANN
660 670 680 690 700
ETVEDIAKRR NIAVEQVNKR EAKAAVVTGM ELKDMTPEQL DELLTNYQEI
710 720 730 740 750
VFARTSPQQK LIIVEGCQRQ DAIVAVTGDG VNDSPALKKA DIGIAMGIAG
760 770 780 790 800
SDAAKNAADM VLLDDNFASI VTGVEEGRLI FDNLKKTIAY TLTKNIAELC
810 820 830 840 850
PFLIYIVAGL PLPIGTITIL FIDLGTDIIP SIALAYEKAE SDIMNRKPRH
860 870 880 890 900
KKKDRLVNTQ LAIYSYLHIG LMQALGGFLV YFTVYAQQGF WPTSLINLRV
910 920 930 940 950
AWETDDINDL EDSYGQEWTR YQRKYLEWTG STAFFVAIMI QQIADLIIRK
960 970 980 990 1000
TRRNSIFQQG LFRNKVIWVG IASQVIVALI LSYGLGSVPA LSFTMLRVQY
1010 1020 1030
WFVAVPHAIL IWVYDEMRKL FIRLYPGSWW DKNMYY
Length:1,036
Mass (Da):114,975
Last modified:October 1, 1996 - v1
Checksum:i453918E65CD4813B
GO
Isoform Short (identifier: P54708-2) [UniParc]FASTAAdd to basket

Also known as: 2b

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Show »
Length:928
Mass (Da):102,562
Checksum:iEB586EC235C4CB85
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 108108Missing in isoform Short. 1 PublicationVSP_000414Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90398 mRNA. Translation: AAA40779.1.
U94911 Genomic DNA. Translation: AAB93900.1.
U94912 mRNA. Translation: AAB93901.1.
U94913 mRNA. Translation: AAB93902.1.
PIRiA42895.
UniGeneiRn.9858.

Genome annotation databases

UCSCiRGD:620569. rat. [P54708-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90398 mRNA. Translation: AAA40779.1.
U94911 Genomic DNA. Translation: AAB93900.1.
U94912 mRNA. Translation: AAB93901.1.
U94913 mRNA. Translation: AAB93902.1.
PIRiA42895.
UniGeneiRn.9858.

3D structure databases

ProteinModelPortaliP54708.
SMRiP54708. Positions 41-1036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028093.

Protein family/group databases

TCDBi3.A.3.1.4. the p-type atpase (p-atpase) superfamily.

PTM databases

PhosphoSiteiP54708.

Proteomic databases

PaxDbiP54708.
PRIDEiP54708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620569. rat. [P54708-1]

Organism-specific databases

RGDi620569. Atp12a.

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP54708.
PhylomeDBiP54708.

Miscellaneous databases

NextBioi621525.
PROiP54708.

Gene expression databases

GenevisibleiP54708. RN.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030318. Atp12a.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF282. PTHR24093:SF282. 1 hit.
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of a cDNA encoding the putative distal colon H+,K(+)-ATPase. Similarity of deduced amino acid sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA expression in distal colon, kidney, and uterus."
    Crowson M.S., Shull G.E.
    J. Biol. Chem. 267:13740-13748(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
    Strain: Sprague-Dawley.
    Tissue: Colon.
  2. "A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2 subunit. Cloning, functional expression, and renal adaptive response to chronic hypokalemia."
    Kone B.C., Higham S.C.
    J. Biol. Chem. 273:2543-2552(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS LONG AND SHORT).
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  3. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 779-785, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit."
    Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N.
    FEBS Lett. 440:320-324(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiAT12A_RAT
AccessioniPrimary (citable) accession number: P54708
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 24, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.