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Reviewed, UniProtKB/Swiss-Prot P54708 (AT12A_RAT)

Last modified October 13, 2009. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Potassium-transporting ATPase alpha chain 2
    EC=3.6.3.10
Alternative name(s):
    Proton pump
    Non-gastric H(+)/K(+) ATPase subunit alpha
Gene names
Name: Atp12a
Synonyms: Atp1al1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length1036 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activity

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Subunit structure

Composed of two subunits: alpha (catalytic) and beta.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed at high levels in distal colon, coagulating and preputial glands; at much lower levels in proximal colon, kidney, uterus, brain, placenta and lung; and at trace levels in heart and forestomach. Ref.4

Sequence similarities

Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P54708-1)

Also known as: 2a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P54708-2)

Also known as: 2b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10361036Potassium-transporting ATPase alpha chain 2
PRO_0000046263

Regions

Topological domain1 – 9999Cytoplasmic Potential
Transmembrane100 – 12021 Potential
Topological domain121 – 14323Lumenal Potential
Transmembrane144 – 16421 Potential
Topological domain165 – 300136Cytoplasmic Potential
Transmembrane301 – 32020 Potential
Topological domain321 – 33212Lumenal Potential
Transmembrane333 – 35018 Potential
Topological domain351 – 784434Cytoplasmic Potential
Transmembrane785 – 80420 Potential
Topological domain805 – 81410Lumenal Potential
Transmembrane815 – 83521 Potential
Topological domain836 – 85520Cytoplasmic Potential
Transmembrane856 – 87823 Potential
Topological domain879 – 93052Lumenal Potential
Transmembrane931 – 95020 Potential
Topological domain951 – 96414Cytoplasmic Potential
Transmembrane965 – 98319 Potential
Topological domain984 – 99815Lumenal Potential
Transmembrane999 – 101921 Potential
Topological domain1020 – 103617Cytoplasmic Potential

Sites

Active site38814-aspartylphosphate intermediate By similarity
Metal binding7291Magnesium By similarity
Metal binding7331Magnesium By similarity

Amino acid modifications

Modified residue9551Phosphoserine; by PKA By similarity

Natural variations

Alternative sequence1 – 108108Missing in isoform Short.
VSP_000414

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Sequences

Sequence LengthMass (Da)Tools
Isoform Long (2a) [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 453918E65CD4813B

FASTA1,036114,975
        10         20         30         40         50         60 
MRRKTEIYSV ELNGTKDVKP ADQRDDKKFK GAKNKDLEPN KSHEKEELKK ELDLDDHRLS 

        70         80         90        100        110        120 
NTELEQKYGT NIIQGLSSVR ATELLARDGP NTLTPPKQTP EIIKFLKQMV GGFSILLWIG 

       130        140        150        160        170        180 
AALCWIAFVI QYVNNSASLD NVYLGAILVL VVILTGIFAY YQEAKSTNIM ASFSKMIPQQ 

       190        200        210        220        230        240 
ALVIRDAEKK VISAEQLVVG DVVEIKGGDQ IPADIRLVFS QGCKVDNSSL TGESEPQARS 

       250        260        270        280        290        300 
TEFTHENPLE TKNIGFYSTT CLEGTATGIV INTGDRTIIG RIASLASGVG SEKTPIAIEI 

       310        320        330        340        350        360 
EHFVHIVAGV AVSIDIIFFI TAVCMKYYVL DAIIFLISII VANVPEGLLA TVTVTLSLTA 

       370        380        390        400        410        420 
KRMAKKNCLV KNLEAVETLG STSIICSDKT GTLTQNRMTV AHLWFDNQIF VADTSENQTK 

       430        440        450        460        470        480 
QAFDQSSGTW ASLSKIITLC NRAEFRPGQE SVPIMKRTVV GDASETALLK FSEVILGDVM 

       490        500        510        520        530        540 
GIRKRNHKVA EIPFNSTNKF QLSIHETEDP NNKRFLVVMK GAPERILEKC STIMINGQEQ 

       550        560        570        580        590        600 
PLDKSSADSF HTAYMELGGL GERVLGFCHL YLPAEQFPQS YIFDVDSVNF PTSNFCFVGL 

       610        620        630        640        650        660 
LSMIDPPRST VPDAVSKCRS AGIKVIMVTG DHPITAKAIA KSVGIISANN ETVEDIAKRR 

       670        680        690        700        710        720 
NIAVEQVNKR EAKAAVVTGM ELKDMTPEQL DELLTNYQEI VFARTSPQQK LIIVEGCQRQ 

       730        740        750        760        770        780 
DAIVAVTGDG VNDSPALKKA DIGIAMGIAG SDAAKNAADM VLLDDNFASI VTGVEEGRLI 

       790        800        810        820        830        840 
FDNLKKTIAY TLTKNIAELC PFLIYIVAGL PLPIGTITIL FIDLGTDIIP SIALAYEKAE 

       850        860        870        880        890        900 
SDIMNRKPRH KKKDRLVNTQ LAIYSYLHIG LMQALGGFLV YFTVYAQQGF WPTSLINLRV 

       910        920        930        940        950        960 
AWETDDINDL EDSYGQEWTR YQRKYLEWTG STAFFVAIMI QQIADLIIRK TRRNSIFQQG 

       970        980        990       1000       1010       1020 
LFRNKVIWVG IASQVIVALI LSYGLGSVPA LSFTMLRVQY WFVAVPHAIL IWVYDEMRKL 

      1030 
FIRLYPGSWW DKNMYY

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Isoform Short (2b).

Checksum: EB586EC235C4CB85
Show »

FASTA928102,562

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References

[1]"Isolation and characterization of a cDNA encoding the putative distal colon H+,K(+)-ATPase. Similarity of deduced amino acid sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA expression in distal colon, kidney, and uterus."
Crowson M.S., Shull G.E.
J. Biol. Chem. 267:13740-13748(1992) [PubMed: 1320029] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
Strain: Sprague-Dawley.
Tissue: Colon.
[2]"A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2 subunit. Cloning, functional expression, and renal adaptive response to chronic hypokalemia."
Kone B.C., Higham S.C.
J. Biol. Chem. 273:2543-2552(1998) [PubMed: 9446555] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS LONG AND SHORT).
Strain: Sprague-Dawley.
Tissue: Kidney.
[3]Lubec G., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 779-785, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[4]"Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit."
Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N.
FEBS Lett. 440:320-324(1998) [PubMed: 9872395] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M90398 mRNA. Translation: AAA40779.1.
U94911 Genomic DNA. Translation: AAB93900.1.
U94912 mRNA. Translation: AAB93901.1.
U94913 mRNA. Translation: AAB93902.1.
IPIIPI00188119.
IPI00231462.
PIRA42895.
RefSeqNP_598201.1.
UniGeneRn.9858

3D structure databases

HSSPHSSP built from PDB template 1MO7 based on UniProtKB P06685.
ModBaseSearch...

Protein-protein interaction databases

STRINGP54708.

Protein family/group databases

TCDB3.A.3.1.4. P-type ATPase (P-ATPase) superfamily.

PTM databases

PhosphoSiteP54708.

Proteomic databases

PRIDEP54708.

Genome annotation databases

EnsemblENSRNOT00000028093; ENSRNOP00000028093; ENSRNOG00000020685; Rattus norvegicus. [Genome view]
GeneID171028.
KEGGrno:171028.
UCSCNM_133517. rat.

Organism-specific databases

CTD171028.
RGD620569. Atp12a.

Phylogenomic databases

HOVERGENP54708.

Enzyme and pathway databases

BRENDA3.6.3.10. 248.

Gene expression databases

ArrayExpressP54708.
GenevestigatorP54708.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-reg.
IPR005775. ATPase_P-typ_cation-ex_asu_euk.
IPR006069. ATPase_P-typ_cation-exchng_asu.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
[Graphical view]
PANTHERPTHR11939. ATPase_P. 1 hit.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00121. NAKATPASE.
TIGRFAMsTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 4 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio621525.

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Entry information

Entry nameAT12A_RAT
AccessionPrimary (citable) accession number: P54708
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 13, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents