Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium-transporting ATPase alpha chain 2

Gene

Atp12a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3884-aspartylphosphate intermediateBy similarity1
Metal bindingi729MagnesiumBy similarity1
Metal bindingi733MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • metal ion transport Source: RGD
  • potassium ion transport Source: RGD
  • response to metal ion Source: RGD
  • response to organic cyclic compound Source: RGD

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Potassium transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Protein family/group databases

TCDBi3.A.3.1.4 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp12a
Synonyms:Atp1al1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620569 Atp12a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 99CytoplasmicSequence analysisAdd BLAST99
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121 – 143LumenalSequence analysisAdd BLAST23
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 300CytoplasmicSequence analysisAdd BLAST136
Transmembranei301 – 320HelicalSequence analysisAdd BLAST20
Topological domaini321 – 332LumenalSequence analysisAdd BLAST12
Transmembranei333 – 350HelicalSequence analysisAdd BLAST18
Topological domaini351 – 784CytoplasmicSequence analysisAdd BLAST434
Transmembranei785 – 804HelicalSequence analysisAdd BLAST20
Topological domaini805 – 814LumenalSequence analysis10
Transmembranei815 – 835HelicalSequence analysisAdd BLAST21
Topological domaini836 – 855CytoplasmicSequence analysisAdd BLAST20
Transmembranei856 – 878HelicalSequence analysisAdd BLAST23
Topological domaini879 – 930LumenalSequence analysisAdd BLAST52
Transmembranei931 – 950HelicalSequence analysisAdd BLAST20
Topological domaini951 – 964CytoplasmicSequence analysisAdd BLAST14
Transmembranei965 – 983HelicalSequence analysisAdd BLAST19
Topological domaini984 – 998LumenalSequence analysisAdd BLAST15
Transmembranei999 – 1019HelicalSequence analysisAdd BLAST21
Topological domaini1020 – 1036CytoplasmicSequence analysisAdd BLAST17

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462631 – 1036Potassium-transporting ATPase alpha chain 2Add BLAST1036

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei955Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54708
PRIDEiP54708

PTM databases

iPTMnetiP54708
PhosphoSitePlusiP54708

Expressioni

Tissue specificityi

Expressed at high levels in distal colon, coagulating and preputial glands; at much lower levels in proximal colon, kidney, uterus, brain, placenta and lung; and at trace levels in heart and forestomach.1 Publication

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028093

Structurei

3D structure databases

ProteinModelPortaliP54708
SMRiP54708
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203 Eukaryota
COG0474 LUCA
HOGENOMiHOG000265622
HOVERGENiHBG004298
InParanoidiP54708
PhylomeDBiP54708

Family and domain databases

CDDicd02608 P-type_ATPase_Na-K_like, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR030318 Atp12a
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005775 P-type_ATPase_IIC
IPR001757 P_typ_ATPase
PANTHERiPTHR43294:SF1 PTHR43294:SF1, 1 hit
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01106 ATPase-IIC_X-K, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P54708-1) [UniParc]FASTAAdd to basket
Also known as: 2a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRKTEIYSV ELNGTKDVKP ADQRDDKKFK GAKNKDLEPN KSHEKEELKK
60 70 80 90 100
ELDLDDHRLS NTELEQKYGT NIIQGLSSVR ATELLARDGP NTLTPPKQTP
110 120 130 140 150
EIIKFLKQMV GGFSILLWIG AALCWIAFVI QYVNNSASLD NVYLGAILVL
160 170 180 190 200
VVILTGIFAY YQEAKSTNIM ASFSKMIPQQ ALVIRDAEKK VISAEQLVVG
210 220 230 240 250
DVVEIKGGDQ IPADIRLVFS QGCKVDNSSL TGESEPQARS TEFTHENPLE
260 270 280 290 300
TKNIGFYSTT CLEGTATGIV INTGDRTIIG RIASLASGVG SEKTPIAIEI
310 320 330 340 350
EHFVHIVAGV AVSIDIIFFI TAVCMKYYVL DAIIFLISII VANVPEGLLA
360 370 380 390 400
TVTVTLSLTA KRMAKKNCLV KNLEAVETLG STSIICSDKT GTLTQNRMTV
410 420 430 440 450
AHLWFDNQIF VADTSENQTK QAFDQSSGTW ASLSKIITLC NRAEFRPGQE
460 470 480 490 500
SVPIMKRTVV GDASETALLK FSEVILGDVM GIRKRNHKVA EIPFNSTNKF
510 520 530 540 550
QLSIHETEDP NNKRFLVVMK GAPERILEKC STIMINGQEQ PLDKSSADSF
560 570 580 590 600
HTAYMELGGL GERVLGFCHL YLPAEQFPQS YIFDVDSVNF PTSNFCFVGL
610 620 630 640 650
LSMIDPPRST VPDAVSKCRS AGIKVIMVTG DHPITAKAIA KSVGIISANN
660 670 680 690 700
ETVEDIAKRR NIAVEQVNKR EAKAAVVTGM ELKDMTPEQL DELLTNYQEI
710 720 730 740 750
VFARTSPQQK LIIVEGCQRQ DAIVAVTGDG VNDSPALKKA DIGIAMGIAG
760 770 780 790 800
SDAAKNAADM VLLDDNFASI VTGVEEGRLI FDNLKKTIAY TLTKNIAELC
810 820 830 840 850
PFLIYIVAGL PLPIGTITIL FIDLGTDIIP SIALAYEKAE SDIMNRKPRH
860 870 880 890 900
KKKDRLVNTQ LAIYSYLHIG LMQALGGFLV YFTVYAQQGF WPTSLINLRV
910 920 930 940 950
AWETDDINDL EDSYGQEWTR YQRKYLEWTG STAFFVAIMI QQIADLIIRK
960 970 980 990 1000
TRRNSIFQQG LFRNKVIWVG IASQVIVALI LSYGLGSVPA LSFTMLRVQY
1010 1020 1030
WFVAVPHAIL IWVYDEMRKL FIRLYPGSWW DKNMYY
Length:1,036
Mass (Da):114,975
Last modified:October 1, 1996 - v1
Checksum:i453918E65CD4813B
GO
Isoform Short (identifier: P54708-2) [UniParc]FASTAAdd to basket
Also known as: 2b

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Show »
Length:928
Mass (Da):102,562
Checksum:iEB586EC235C4CB85
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0004141 – 108Missing in isoform Short. 1 PublicationAdd BLAST108

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90398 mRNA Translation: AAA40779.1
U94911 Genomic DNA Translation: AAB93900.1
U94912 mRNA Translation: AAB93901.1
U94913 mRNA Translation: AAB93902.1
PIRiA42895
UniGeneiRn.9858

Genome annotation databases

UCSCiRGD:620569 rat [P54708-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAT12A_RAT
AccessioniPrimary (citable) accession number: P54708
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health