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P54708

- AT12A_RAT

UniProt

P54708 - AT12A_RAT

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Protein

Potassium-transporting ATPase alpha chain 2

Gene
Atp12a, Atp1al1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei388 – 38814-aspartylphosphate intermediate By similarity
Metal bindingi729 – 7291Magnesium By similarity
Metal bindingi733 – 7331Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrogen:potassium-exchanging ATPase activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. hydrogen ion transmembrane transport Source: GOC
  3. metal ion transport Source: RGD
  4. potassium ion transport Source: RGD
  5. response to metal ion Source: RGD
  6. response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Protein family/group databases

TCDBi3.A.3.1.4. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp12a
Synonyms:Atp1al1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620569. Atp12a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9999Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei100 – 12021Helical; Reviewed predictionAdd
BLAST
Topological domaini121 – 14323Lumenal Reviewed predictionAdd
BLAST
Transmembranei144 – 16421Helical; Reviewed predictionAdd
BLAST
Topological domaini165 – 300136Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei301 – 32020Helical; Reviewed predictionAdd
BLAST
Topological domaini321 – 33212Lumenal Reviewed predictionAdd
BLAST
Transmembranei333 – 35018Helical; Reviewed predictionAdd
BLAST
Topological domaini351 – 784434Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei785 – 80420Helical; Reviewed predictionAdd
BLAST
Topological domaini805 – 81410Lumenal Reviewed prediction
Transmembranei815 – 83521Helical; Reviewed predictionAdd
BLAST
Topological domaini836 – 85520Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei856 – 87823Helical; Reviewed predictionAdd
BLAST
Topological domaini879 – 93052Lumenal Reviewed predictionAdd
BLAST
Transmembranei931 – 95020Helical; Reviewed predictionAdd
BLAST
Topological domaini951 – 96414Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei965 – 98319Helical; Reviewed predictionAdd
BLAST
Topological domaini984 – 99815Lumenal Reviewed predictionAdd
BLAST
Transmembranei999 – 101921Helical; Reviewed predictionAdd
BLAST
Topological domaini1020 – 103617Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Potassium-transporting ATPase alpha chain 2PRO_0000046263Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei955 – 9551Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54708.
PRIDEiP54708.

PTM databases

PhosphoSiteiP54708.

Expressioni

Tissue specificityi

Expressed at high levels in distal colon, coagulating and preputial glands; at much lower levels in proximal colon, kidney, uterus, brain, placenta and lung; and at trace levels in heart and forestomach.1 Publication

Gene expression databases

GenevestigatoriP54708.

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028093.

Structurei

3D structure databases

ProteinModelPortaliP54708.
SMRiP54708. Positions 41-1036.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP54708.
KOiK01544.
PhylomeDBiP54708.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P54708-1) [UniParc]FASTAAdd to Basket

Also known as: 2a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRRKTEIYSV ELNGTKDVKP ADQRDDKKFK GAKNKDLEPN KSHEKEELKK     50
ELDLDDHRLS NTELEQKYGT NIIQGLSSVR ATELLARDGP NTLTPPKQTP 100
EIIKFLKQMV GGFSILLWIG AALCWIAFVI QYVNNSASLD NVYLGAILVL 150
VVILTGIFAY YQEAKSTNIM ASFSKMIPQQ ALVIRDAEKK VISAEQLVVG 200
DVVEIKGGDQ IPADIRLVFS QGCKVDNSSL TGESEPQARS TEFTHENPLE 250
TKNIGFYSTT CLEGTATGIV INTGDRTIIG RIASLASGVG SEKTPIAIEI 300
EHFVHIVAGV AVSIDIIFFI TAVCMKYYVL DAIIFLISII VANVPEGLLA 350
TVTVTLSLTA KRMAKKNCLV KNLEAVETLG STSIICSDKT GTLTQNRMTV 400
AHLWFDNQIF VADTSENQTK QAFDQSSGTW ASLSKIITLC NRAEFRPGQE 450
SVPIMKRTVV GDASETALLK FSEVILGDVM GIRKRNHKVA EIPFNSTNKF 500
QLSIHETEDP NNKRFLVVMK GAPERILEKC STIMINGQEQ PLDKSSADSF 550
HTAYMELGGL GERVLGFCHL YLPAEQFPQS YIFDVDSVNF PTSNFCFVGL 600
LSMIDPPRST VPDAVSKCRS AGIKVIMVTG DHPITAKAIA KSVGIISANN 650
ETVEDIAKRR NIAVEQVNKR EAKAAVVTGM ELKDMTPEQL DELLTNYQEI 700
VFARTSPQQK LIIVEGCQRQ DAIVAVTGDG VNDSPALKKA DIGIAMGIAG 750
SDAAKNAADM VLLDDNFASI VTGVEEGRLI FDNLKKTIAY TLTKNIAELC 800
PFLIYIVAGL PLPIGTITIL FIDLGTDIIP SIALAYEKAE SDIMNRKPRH 850
KKKDRLVNTQ LAIYSYLHIG LMQALGGFLV YFTVYAQQGF WPTSLINLRV 900
AWETDDINDL EDSYGQEWTR YQRKYLEWTG STAFFVAIMI QQIADLIIRK 950
TRRNSIFQQG LFRNKVIWVG IASQVIVALI LSYGLGSVPA LSFTMLRVQY 1000
WFVAVPHAIL IWVYDEMRKL FIRLYPGSWW DKNMYY 1036
Length:1,036
Mass (Da):114,975
Last modified:October 1, 1996 - v1
Checksum:i453918E65CD4813B
GO
Isoform Short (identifier: P54708-2) [UniParc]FASTAAdd to Basket

Also known as: 2b

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Show »
Length:928
Mass (Da):102,562
Checksum:iEB586EC235C4CB85
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 108108Missing in isoform Short. VSP_000414Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90398 mRNA. Translation: AAA40779.1.
U94911 Genomic DNA. Translation: AAB93900.1.
U94912 mRNA. Translation: AAB93901.1.
U94913 mRNA. Translation: AAB93902.1.
PIRiA42895.
RefSeqiNP_598201.1. NM_133517.1. [P54708-1]
UniGeneiRn.9858.

Genome annotation databases

GeneIDi171028.
KEGGirno:171028.
UCSCiRGD:620569. rat. [P54708-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M90398 mRNA. Translation: AAA40779.1 .
U94911 Genomic DNA. Translation: AAB93900.1 .
U94912 mRNA. Translation: AAB93901.1 .
U94913 mRNA. Translation: AAB93902.1 .
PIRi A42895.
RefSeqi NP_598201.1. NM_133517.1. [P54708-1 ]
UniGenei Rn.9858.

3D structure databases

ProteinModelPortali P54708.
SMRi P54708. Positions 41-1036.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000028093.

Protein family/group databases

TCDBi 3.A.3.1.4. the p-type atpase (p-atpase) superfamily.

PTM databases

PhosphoSitei P54708.

Proteomic databases

PaxDbi P54708.
PRIDEi P54708.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 171028.
KEGGi rno:171028.
UCSCi RGD:620569. rat. [P54708-1 ]

Organism-specific databases

CTDi 479.
RGDi 620569. Atp12a.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265622.
HOVERGENi HBG004298.
InParanoidi P54708.
KOi K01544.
PhylomeDBi P54708.

Miscellaneous databases

NextBioi 621525.

Gene expression databases

Genevestigatori P54708.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of a cDNA encoding the putative distal colon H+,K(+)-ATPase. Similarity of deduced amino acid sequence to gastric H+,K(+)-ATPase and Na+,K(+)-ATPase and mRNA expression in distal colon, kidney, and uterus."
    Crowson M.S., Shull G.E.
    J. Biol. Chem. 267:13740-13748(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM LONG).
    Strain: Sprague-Dawley.
    Tissue: Colon.
  2. "A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2 subunit. Cloning, functional expression, and renal adaptive response to chronic hypokalemia."
    Kone B.C., Higham S.C.
    J. Biol. Chem. 273:2543-2552(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS LONG AND SHORT).
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  3. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 779-785, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit."
    Pestov N.B., Romanova L.G., Korneenko T.V., Egorov M.V., Kostina M.B., Sverdlov V.E., Askari A., Shakhparonov M.I., Modyanov N.N.
    FEBS Lett. 440:320-324(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiAT12A_RAT
AccessioniPrimary (citable) accession number: P54708
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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