Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin-J heavy chain

Gene

myoJ

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Processive motor protein that can move over long distances along F-actin without disassociating; processiveness depends on high physiological Mg2+ concentrations. Presents a high actin affinity in the presence of ADP, fast ATP hydrolysis, and a high steady-state ATPase activity in the presence of actin that is rate limited by ADP release. Physiological decrease of free Mg2+ ions leads to an increased rate of ADP release and shortening of the fraction of time it spends in the strong acting binding states.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 181ATPSequence analysis8

GO - Molecular functioni

  • actin-dependent ATPase activity Source: dictyBase
  • actin filament binding Source: dictyBase
  • ADP binding Source: dictyBase
  • ATP binding Source: dictyBase
  • microfilament motor activity Source: dictyBase

GO - Biological processi

  • actin-myosin filament sliding Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-J heavy chain
Alternative name(s):
Myosin-5b
Gene namesi
Name:myoJ
Synonyms:myo5B
ORF Names:DDB_G0272112
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0272112. myoJ.

Subcellular locationi

GO - Cellular componenti

  • actomyosin Source: dictyBase
  • contractile vacuole Source: dictyBase
  • myosin complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1781867.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001233721 – 2245Myosin-J heavy chainAdd BLAST2245

Proteomic databases

PaxDbiP54697.

Interactioni

Subunit structurei

Homodimer that associates with six light chains.

GO - Molecular functioni

  • actin filament binding Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0185050.

Chemistry databases

BindingDBiP54697.

Structurei

3D structure databases

ProteinModelPortaliP54697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 821Myosin motorAdd BLAST741
Domaini824 – 851IQ 1PROSITE-ProRule annotationAdd BLAST28
Domaini872 – 901IQ 2PROSITE-ProRule annotationAdd BLAST30
Domaini943 – 972IQ 3PROSITE-ProRule annotationAdd BLAST30
Domaini1969 – 2188DilutePROSITE-ProRule annotationAdd BLAST220

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni669 – 749Actin-bindingAdd BLAST81

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili973 – 1812Sequence analysisAdd BLAST840

Sequence similaritiesi

Contains 1 dilute domain.PROSITE-ProRule annotation
Contains 3 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
COG5022. LUCA.
InParanoidiP54697.
PhylomeDBiP54697.

Family and domain databases

InterProiIPR002710. Dilute_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00612. IQ. 3 hits.
PF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM01132. DIL. 1 hit.
SM00015. IQ. 3 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51126. DILUTE. 1 hit.
PS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSTIENGA SSPIIVSSST PKLYQEGAGV WIPDQELGWI GADVIEHSET
60 70 80 90 100
SADQVLVRTE DDREVKIPLS KVFQKNPDIL EGVDDLSFLS HLHEPAILHN
110 120 130 140 150
LHHRYNLNQI YTYIGKILIA INPYTSLPLY GKEMISAYYG KQLGTLAPHV
160 170 180 190 200
YAVAEDAFKD MRYDGTSQSI LVSGESGAGK TETTKFLLQY FAAMGNMIKE
210 220 230 240 250
STSSSSINGI NTSSDGIPVT PPPSPMKKSP VDKSVEERVL ESTPLLEAFG
260 270 280 290 300
NAKTLRNDNS SRFGKFIEIH FNEMGSIIGA KILTYLLEKS RIVRQVYNER
310 320 330 340 350
NYHIFYQLLS GASEELKEKL NLKTIEEYSY LNKSGCFEIE GVSDEEHFNK
360 370 380 390 400
TCHAMQVAGI TLVEQENVFR ILSAILLIGN FEFENIAGSN DDSCQLIDRD
410 420 430 440 450
PLEKVSVLLG CAQPDELLNS MLTRKVVTGK ESYISHNTKE RAENARDSLS
460 470 480 490 500
MFLYGMMFDW LVVKINSSMS ISTQQKSKSF IGVLDIYGFE SFEVNGFEQF
510 520 530 540 550
CINYANEKLQ QLFNQHVFKE EQQEYIKEKI DWSYIDFNDN QDTLDLIEKK
560 570 580 590 600
PICILTLLDE ETMFPKATPQ TLATKLYSKM TSHSKFEKPR FSSTAFTINH
610 620 630 640 650
YAGKVTYETD QFLDKNKDFI IPEQISILQR SNFSFIKVLM SHSDKFTQSP
660 670 680 690 700
GGHPQGNGGP TSSNTKGTSG SSSMKFLSVG SQFSTSLATL MKTISTTTPH
710 720 730 740 750
YVRCIKPNPE KLPQTFNKQD VIHQLRCGGV MESVRICCAG FPTRRLLSEF
760 770 780 790 800
YQRYKILYVK DINTGSGGGK KGSNNNKIKD PKILVQNLLT GIELSDDKYK
810 820 830 840 850
IGLTKVFLRA GQLASLEDMR LEQLDRSATV IQKRWKGYLY RKRYKQLRDA
860 870 880 890 900
SLIIQTKLRS VHAKQQLSAL QRTHSAILIQ KVWRAHRDRV QYQKIRDASL
910 920 930 940 950
QLQTVMRRHL FSEQVHRERC ENAAIILQTK IRQILSKREV DKKLRGIILI
960 970 980 990 1000
QARWRMKLAK RVYIQLRAEA RSLRTVQEQK NKLQEKLEEL QWRLTSEAKR
1010 1020 1030 1040 1050
KQQLEDQKVK SDTTISELSS NNDHLELQLS EIQLKYQELD KSNQSSQLQL
1060 1070 1080 1090 1100
SECLSKLEEQ TQQLDHSSKL NKKLEKDLSD QHDSIEKLQS QFNETEQQLQ
1110 1120 1130 1140 1150
QFKQQSEELS SKLSKTTQQL DFNKQEFDRL SQERDTDNTN NQLEIQQLKK
1160 1170 1180 1190 1200
ANSTLEEDYF SLSGIRDNLE RQVLELRDEN QLIKERLDSL GQQSSQFQSG
1210 1220 1230 1240 1250
AALEKQQLEQ LVQEQSEQLI KLSSEKLGSE EEAKKQINQL ELELTDHKSK
1260 1270 1280 1290 1300
LQIQLQLTEQ SNEKIKKLKG KLEEYQDEKK QLQQELERIK QSKQSVEDEK
1310 1320 1330 1340 1350
NSLITQLTTV KFESTQVSTN VSHQKEKITT LKSTIEELNK SIGKLQAEQK
1360 1370 1380 1390 1400
NKDDEIRKIQ FELNDQKQQF TRQTKEFSDL QSQQSIDRQK SEITIHSLER
1410 1420 1430 1440 1450
TNETLKSDFE RVQQSLKQQE RDCQQYKDTI NRLENEVKQL TQLKERFENE
1460 1470 1480 1490 1500
FFVAKEQNSN QTQESVYLKE VTTQMQQNQS RIERELEEKK QHITRIDDER
1510 1520 1530 1540 1550
DELKKQLTQL QQQHEQSSTQ LLLAQNELER LRKKELKYKE RGHETSKQQD
1560 1570 1580 1590 1600
QFNMEIQSLR ITNNDQLKSL QDYEQEKKKL KDKLSSSKQE AQQQRESIIK
1610 1620 1630 1640 1650
MDAELSAIKQ HSQWVENSFT DMKQRNQELI ESSALYKQQL LQQTSTIDST
1660 1670 1680 1690 1700
IKEKENEISK LQQQLETSNQ QLHQLKEELN SMKQSNQLES TEQSKQLNQL
1710 1720 1730 1740 1750
IQENQQLKSV TNEISKQLDD AVFENQKINN TIKEQEIKSK RMSVELQQHI
1760 1770 1780 1790 1800
DEGKQQEIQQ LQSTIAQLKQ QQQSETDRLE KEIQQMKRER ETQMKLVEST
1810 1820 1830 1840 1850
KLNYHMLEDR MELYRNVMEI IDYKETEWEK LARLAGCKEL DTKLLSDFLL
1860 1870 1880 1890 1900
SCKLEHTSLG SQMWFHQIDY WCPYERDSSK GIFYGIIRSI VDFTIKNFDD
1910 1920 1930 1940 1950
VDLLSYLLAC CSLTLFLYKK NLVKHLNGAN SIMPIIPTLG DLEELNERLS
1960 1970 1980 1990 2000
HQSLTTSGKF SGGGGGGGID FIDQLQQSTG ITFGLIFKAT TLKLSPLVDG
2010 2020 2030 2040 2050
AILNENYNKK LTSISASSFG SGSFGLGSNG VGSVLSIELI TTYLSSIITI
2060 2070 2080 2090 2100
FQHRMVHFTL SQRFFNQVFC WIGALIMKGF MLRQTFCTET FATFVKTKID
2110 2120 2130 2140 2150
FLTRWADDIG NVWVGDVANA FQQVREVINV LNIKDKEKII DDKIRKQYCP
2160 2170 2180 2190 2200
TLNSNQLKQV LSLFSPGEFG GKRVSAKVIA SICPPNKSSA GQSFVQDENK
2210 2220 2230 2240
LNTIPIDSLH YLEIQDIKTL SLPLSIRQTI ETEIINLKQQ IACKK
Length:2,245
Mass (Da):258,684
Last modified:December 4, 2007 - v2
Checksum:i4DA40956515A7685
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti191F → L in AAA85186 (PubMed:8636147).Curated1
Sequence conflicti284T → A in AAA85186 (PubMed:8636147).Curated1
Sequence conflicti291R → G in AAA85186 (PubMed:8636147).Curated1
Sequence conflicti332 – 347NKSGC…SDEEH → IEWMFELKVYRMKS in AAA79858 (PubMed:8884597).CuratedAdd BLAST16
Sequence conflicti550K → N in AAA85186 (PubMed:8636147).Curated1
Sequence conflicti865 – 866QQ → HH in AAA85186 (PubMed:8636147).Curated2
Sequence conflicti1041K → N in AAA85186 (PubMed:8636147).Curated1
Sequence conflicti1389Q → P in AAA85186 (PubMed:8636147).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42409 Genomic DNA. Translation: AAA85186.1.
AAFI02000008 Genomic DNA. Translation: EAL71208.1.
L35322 Genomic DNA. Translation: AAA79858.1.
PIRiT18278.
RefSeqiXP_645195.1. XM_640103.1.

Genome annotation databases

EnsemblProtistsiEAL71208; EAL71208; DDB_G0272112.
GeneIDi8618367.
KEGGiddi:DDB_G0272112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42409 Genomic DNA. Translation: AAA85186.1.
AAFI02000008 Genomic DNA. Translation: EAL71208.1.
L35322 Genomic DNA. Translation: AAA79858.1.
PIRiT18278.
RefSeqiXP_645195.1. XM_640103.1.

3D structure databases

ProteinModelPortaliP54697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0185050.

Chemistry databases

BindingDBiP54697.
ChEMBLiCHEMBL1781867.

Proteomic databases

PaxDbiP54697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL71208; EAL71208; DDB_G0272112.
GeneIDi8618367.
KEGGiddi:DDB_G0272112.

Organism-specific databases

dictyBaseiDDB_G0272112. myoJ.

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
COG5022. LUCA.
InParanoidiP54697.
PhylomeDBiP54697.

Miscellaneous databases

PROiP54697.

Family and domain databases

InterProiIPR002710. Dilute_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01843. DIL. 1 hit.
PF00612. IQ. 3 hits.
PF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM01132. DIL. 1 hit.
SM00015. IQ. 3 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51126. DILUTE. 1 hit.
PS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYOJ_DICDI
AccessioniPrimary (citable) accession number: P54697
Secondary accession number(s): Q559Y9, Q86A36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.