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Protein

Branched-chain-amino-acid aminotransferase, cytosolic

Gene

Bcat1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-RNO-70895. Branched-chain amino acid catabolism.
SABIO-RKP54690.

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase, cytosolic (EC:2.6.1.42)
Short name:
BCAT(c)
Gene namesi
Name:Bcat1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2195. Bcat1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4678.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Branched-chain-amino-acid aminotransferase, cytosolicPRO_0000103294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei247 – 2471N6-(pyridoxal phosphate)lysineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP54690.
PRIDEiP54690.

PTM databases

iPTMnetiP54690.
PhosphoSiteiP54690.
SwissPalmiP54690.

Expressioni

Tissue specificityi

Brain, low expression in ovary and placenta, but not found in liver, kidney, and skeletal muscle.

Gene expression databases

ExpressionAtlasiP54690. baseline and differential.
GenevisibleiP54690. RN.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

  • identical protein binding Source: RGD

Protein-protein interaction databases

BioGridi248224. 1 interaction.
STRINGi10116.ENSRNOP00000021193.

Chemistry

BindingDBiP54690.

Structurei

3D structure databases

ProteinModelPortaliP54690.
SMRiP54690. Positions 49-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 316Poly-Glu

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
GeneTreeiENSGT00390000009532.
HOGENOMiHOG000276704.
HOVERGENiHBG050678.
InParanoidiP54690.
KOiK00826.
OMAiNFFGITH.
OrthoDBiEOG7NGQB7.
PhylomeDBiP54690.
TreeFamiTF300882.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYLSRATAT LARQDCSNGC SASYAEEEEL EASTESYDEE GGSEASTQTF
60 70 80 90 100
RAKDLIITKA DVLKKKPDPS SLVFGASFTD HMLMVEWTSK YGWDKPHIKP
110 120 130 140 150
FENLSIHPAA SVLHYAVELF EGLKAFRGVD NKIRLFRPDL NMKRMCRSAV
160 170 180 190 200
RTTLPEFDKE ELLQCVLQLI QLDREWVPYS TSASLYIRPT FIGIEPSLGV
210 220 230 240 250
KKPSKALLFV ILSPVGSYFS NGTFSPVSLW ANPKFVRSWK GGTGDFKMGC
260 270 280 290 300
NYGSSLLAQC EAAENGCHQV LWLYGKENRI TEVGTMNLFL YWINKDGEEE
310 320 330 340 350
LATPPLDGVI LPGVTRQSIL ELGEEWGEFK VCERHITMDD LSTALEENRV
360 370 380 390 400
KEMFGSGTAC VVCPVASILY KGQMLHIPTM ENGHKLSSRI MAKLTDIQYG
410
RIKSEWTLEL P
Length:411
Mass (Da):46,046
Last modified:October 1, 1996 - v1
Checksum:i98030737376F1DB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35774 mRNA. Translation: AAC52385.1.
BC087710 mRNA. Translation: AAH87710.1.
RefSeqiNP_058949.1. NM_017253.2.
UniGeneiRn.8273.

Genome annotation databases

EnsembliENSRNOT00000021193; ENSRNOP00000021193; ENSRNOG00000015514.
GeneIDi29592.
KEGGirno:29592.
UCSCiRGD:2195. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35774 mRNA. Translation: AAC52385.1.
BC087710 mRNA. Translation: AAH87710.1.
RefSeqiNP_058949.1. NM_017253.2.
UniGeneiRn.8273.

3D structure databases

ProteinModelPortaliP54690.
SMRiP54690. Positions 49-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248224. 1 interaction.
STRINGi10116.ENSRNOP00000021193.

Chemistry

BindingDBiP54690.
ChEMBLiCHEMBL4678.

PTM databases

iPTMnetiP54690.
PhosphoSiteiP54690.
SwissPalmiP54690.

Proteomic databases

PaxDbiP54690.
PRIDEiP54690.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021193; ENSRNOP00000021193; ENSRNOG00000015514.
GeneIDi29592.
KEGGirno:29592.
UCSCiRGD:2195. rat.

Organism-specific databases

CTDi586.
RGDi2195. Bcat1.

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
GeneTreeiENSGT00390000009532.
HOGENOMiHOG000276704.
HOVERGENiHBG050678.
InParanoidiP54690.
KOiK00826.
OMAiNFFGITH.
OrthoDBiEOG7NGQB7.
PhylomeDBiP54690.
TreeFamiTF300882.

Enzyme and pathway databases

ReactomeiR-RNO-70895. Branched-chain amino acid catabolism.
SABIO-RKP54690.

Miscellaneous databases

NextBioi609729.
PROiP54690.

Gene expression databases

ExpressionAtlasiP54690. baseline and differential.
GenevisibleiP54690. RN.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of the mammalian cytosolic branched chain aminotransferase isoenzyme."
    Hutson S.M., Bledsoe R.K., Hall T.R., Dawson P.A.
    J. Biol. Chem. 270:30344-30352(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 394-401, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiBCAT1_RAT
AccessioniPrimary (citable) accession number: P54690
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.