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Protein

Cyclin-dependent kinase 7

Gene

cdk7

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic part of CAK which activates cyclin-associated CDK1/CDK2/CDK4 by threonine phosphorylation, thereby allowing MPF activation.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei130Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 1939.
ReactomeiR-DDI-113418. Formation of the Early Elongation Complex.
R-DDI-5696395. Formation of Incision Complex in GG-NER.
R-DDI-674695. RNA Polymerase II Pre-transcription Events.
R-DDI-6781823. Formation of TC-NER Pre-Incision Complex.
R-DDI-6782135. Dual incision in TC-NER.
R-DDI-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DDI-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-DDI-6807505. RNA polymerase II transcribes snRNA genes.
R-DDI-72086. mRNA Capping.
R-DDI-73776. RNA Polymerase II Promoter Escape.
R-DDI-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-DDI-75953. RNA Polymerase II Transcription Initiation.
R-DDI-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-DDI-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 7 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
CDK-activating kinase
Short name:
CAK
Cell division protein kinase 7
MO15 homolog
Gene namesi
Name:cdk7
Synonyms:cdcC, cdcD, mo15
ORF Names:DDB_G0285417
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285417. cdk7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857961 – 360Cyclin-dependent kinase 7Add BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineBy similarity1
Modified residuei163PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54685.
PRIDEiP54685.

Expressioni

Developmental stagei

Only expressed during vegetative cell growth.

Interactioni

Subunit structurei

Probably associates with cyclin H and mat1 to form a multimeric active enzyme.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191429.

Structurei

3D structure databases

ProteinModelPortaliP54685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 288Protein kinasePROSITE-ProRule annotationAdd BLAST285

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi326 – 342Poly-GlnAdd BLAST17

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0659. Eukaryota.
ENOG410XQDH. LUCA.
InParanoidiP54685.
KOiK02202.
OMAiWHSEPKP.
PhylomeDBiP54685.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKYNIEALI GEGTYGVVSR ATVKATGQIV AIKKIRKILI QNQTDDGINF
60 70 80 90 100
SAIREIKILQ ELKHDNVVNL LDIFAHKSNV YLVFELMQWD LQEVIEDKSI
110 120 130 140 150
ILKPADIKSY MKMLLQGIEA CHRNWVLHRD LKPNNLLMSI NGDLKLADFG
160 170 180 190 200
LARQYGSPNK VFSPQAVTIF YRAPELLFGA KSYGPSVDIW SIGCIFAELM
210 220 230 240 250
LRTPYLPGTG EIDQLRKICS ALGTPNESNW PGVTCLPNYI KFTDHPATPF
260 270 280 290 300
KQLFTAASDE AIDLISKMLL FNPSNRISAA DALNHPYFTS GVKHTNPADL
310 320 330 340 350
PVPFAKKASL LQQRQVLAQV QQQLLQKQQQ QQQQQQQQIQ SQPEPIQVDN
360
VEQTQQAQQV
Length:360
Mass (Da):40,567
Last modified:December 4, 2007 - v2
Checksum:iF6FE0441F21D6B37
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti360V → VKK in AAB35208 (PubMed:7662315).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79590 mRNA. Translation: AAB35208.2.
AAFI02000079 Genomic DNA. Translation: EAL64546.1.
RefSeqiXP_638229.1. XM_633137.1.

Genome annotation databases

EnsemblProtistsiEAL64546; EAL64546; DDB_G0285417.
GeneIDi8625277.
KEGGiddi:DDB_G0285417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79590 mRNA. Translation: AAB35208.2.
AAFI02000079 Genomic DNA. Translation: EAL64546.1.
RefSeqiXP_638229.1. XM_633137.1.

3D structure databases

ProteinModelPortaliP54685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191429.

Proteomic databases

PaxDbiP54685.
PRIDEiP54685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL64546; EAL64546; DDB_G0285417.
GeneIDi8625277.
KEGGiddi:DDB_G0285417.

Organism-specific databases

dictyBaseiDDB_G0285417. cdk7.

Phylogenomic databases

eggNOGiKOG0659. Eukaryota.
ENOG410XQDH. LUCA.
InParanoidiP54685.
KOiK02202.
OMAiWHSEPKP.
PhylomeDBiP54685.

Enzyme and pathway databases

BRENDAi2.7.11.22. 1939.
ReactomeiR-DDI-113418. Formation of the Early Elongation Complex.
R-DDI-5696395. Formation of Incision Complex in GG-NER.
R-DDI-674695. RNA Polymerase II Pre-transcription Events.
R-DDI-6781823. Formation of TC-NER Pre-Incision Complex.
R-DDI-6782135. Dual incision in TC-NER.
R-DDI-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DDI-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-DDI-6807505. RNA polymerase II transcribes snRNA genes.
R-DDI-72086. mRNA Capping.
R-DDI-73776. RNA Polymerase II Promoter Escape.
R-DDI-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-DDI-75953. RNA Polymerase II Transcription Initiation.
R-DDI-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-DDI-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiP54685.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDK7_DICDI
AccessioniPrimary (citable) accession number: P54685
Secondary accession number(s): Q54MR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.