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Protein

Cyclin-dependent kinase 7

Gene

cdk7

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic part of CAK which activates cyclin-associated CDK1/CDK2/CDK4 by threonine phosphorylation, thereby allowing MPF activation.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei130 – 1301Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. meiotic cell cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 7 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
CDK-activating kinase
Short name:
CAK
Cell division protein kinase 7
MO15 homolog
Gene namesi
Name:cdk7
Synonyms:cdcC, cdcD, mo15
ORF Names:DDB_G0285417
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285417. cdk7.

Subcellular locationi

  1. Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Cyclin-dependent kinase 7PRO_0000085796Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571PhosphoserineBy similarity
Modified residuei163 – 1631PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP54685.

Expressioni

Developmental stagei

Only expressed during vegetative cell growth.

Interactioni

Subunit structurei

Probably associates with cyclin H and mat1 to form a multimeric active enzyme.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0191429.

Structurei

3D structure databases

ProteinModelPortaliP54685.
SMRiP54685. Positions 8-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 288285Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi326 – 34217Poly-GlnAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP54685.
KOiK02202.
OMAiQRITIQQ.
PhylomeDBiP54685.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKYNIEALI GEGTYGVVSR ATVKATGQIV AIKKIRKILI QNQTDDGINF
60 70 80 90 100
SAIREIKILQ ELKHDNVVNL LDIFAHKSNV YLVFELMQWD LQEVIEDKSI
110 120 130 140 150
ILKPADIKSY MKMLLQGIEA CHRNWVLHRD LKPNNLLMSI NGDLKLADFG
160 170 180 190 200
LARQYGSPNK VFSPQAVTIF YRAPELLFGA KSYGPSVDIW SIGCIFAELM
210 220 230 240 250
LRTPYLPGTG EIDQLRKICS ALGTPNESNW PGVTCLPNYI KFTDHPATPF
260 270 280 290 300
KQLFTAASDE AIDLISKMLL FNPSNRISAA DALNHPYFTS GVKHTNPADL
310 320 330 340 350
PVPFAKKASL LQQRQVLAQV QQQLLQKQQQ QQQQQQQQIQ SQPEPIQVDN
360
VEQTQQAQQV
Length:360
Mass (Da):40,567
Last modified:December 4, 2007 - v2
Checksum:iF6FE0441F21D6B37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti360 – 3601V → VKK in AAB35208 (PubMed:7662315).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79590 mRNA. Translation: AAB35208.2.
AAFI02000079 Genomic DNA. Translation: EAL64546.1.
RefSeqiXP_638229.1. XM_633137.1.

Genome annotation databases

EnsemblProtistsiDDB0191429; DDB0191429; DDB_G0285417.
GeneIDi8625277.
KEGGiddi:DDB_G0285417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79590 mRNA. Translation: AAB35208.2.
AAFI02000079 Genomic DNA. Translation: EAL64546.1.
RefSeqiXP_638229.1. XM_633137.1.

3D structure databases

ProteinModelPortaliP54685.
SMRiP54685. Positions 8-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0191429.

Proteomic databases

PRIDEiP54685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191429; DDB0191429; DDB_G0285417.
GeneIDi8625277.
KEGGiddi:DDB_G0285417.

Organism-specific databases

dictyBaseiDDB_G0285417. cdk7.

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP54685.
KOiK02202.
OMAiQRITIQQ.
PhylomeDBiP54685.

Enzyme and pathway databases

BRENDAi2.7.11.22. 1939.

Miscellaneous databases

PROiP54685.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A Dictyostelium discoideum gene, which is highly related to mo15 from Xenopus, is expressed during growth but not during development."
    Michaelis C.E., Luo Q., Weeks G.
    Biochem. Cell Biol. 73:51-58(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: V12M2.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiCDK7_DICDI
AccessioniPrimary (citable) accession number: P54685
Secondary accession number(s): Q54MR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: January 7, 2015
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.