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P54685 (CDK7_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 7

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
CDK-activating kinase
Short name=CAK
Cell division protein kinase 7
MO15 homolog
Gene names
Name:cdk7
Synonyms:cdcC, cdcD, mo15
ORF Names:DDB_G0285417
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalytic part of CAK which activates cyclin-associated CDK1/CDK2/CDK4 by threonine phosphorylation, thereby allowing MPF activation By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Subunit structure

Probably associates with cyclin H and mat1 to form a multimeric active enzyme By similarity.

Subcellular location

Nucleus By similarity.

Developmental stage

Only expressed during vegetative cell growth.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Cyclin-dependent kinase 7
PRO_0000085796

Regions

Domain4 – 288285Protein kinase
Nucleotide binding10 – 189ATP By similarity
Compositional bias326 – 34217Poly-Gln

Sites

Active site1301Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue1571Phosphoserine By similarity
Modified residue1631Phosphoserine By similarity

Experimental info

Sequence conflict3601V → VKK in AAB35208. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54685 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: F6FE0441F21D6B37

FASTA36040,567
        10         20         30         40         50         60 
MDKYNIEALI GEGTYGVVSR ATVKATGQIV AIKKIRKILI QNQTDDGINF SAIREIKILQ 

        70         80         90        100        110        120 
ELKHDNVVNL LDIFAHKSNV YLVFELMQWD LQEVIEDKSI ILKPADIKSY MKMLLQGIEA 

       130        140        150        160        170        180 
CHRNWVLHRD LKPNNLLMSI NGDLKLADFG LARQYGSPNK VFSPQAVTIF YRAPELLFGA 

       190        200        210        220        230        240 
KSYGPSVDIW SIGCIFAELM LRTPYLPGTG EIDQLRKICS ALGTPNESNW PGVTCLPNYI 

       250        260        270        280        290        300 
KFTDHPATPF KQLFTAASDE AIDLISKMLL FNPSNRISAA DALNHPYFTS GVKHTNPADL 

       310        320        330        340        350        360 
PVPFAKKASL LQQRQVLAQV QQQLLQKQQQ QQQQQQQQIQ SQPEPIQVDN VEQTQQAQQV 

« Hide

References

« Hide 'large scale' references
[1]"A Dictyostelium discoideum gene, which is highly related to mo15 from Xenopus, is expressed during growth but not during development."
Michaelis C.E., Luo Q., Weeks G.
Biochem. Cell Biol. 73:51-58(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: V12M2.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S79590 mRNA. Translation: AAB35208.2.
AAFI02000079 Genomic DNA. Translation: EAL64546.1.

3D structure databases

ProteinModelPortalP54685.
SMRP54685. Positions 8-303.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191429.

Proteomic databases

PRIDEP54685.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191429; DDB0191429; DDB_G0285417.
KEGGddi:DDB_G0285417.

Organism-specific databases

dictyBaseDDB_G0285417. cdk7.

Phylogenomic databases

eggNOGCOG0515.
KOK02202.
OMATYAVVYR.
PhylomeDBP54685.
ProtClustDBCLSZ2728924.

Enzyme and pathway databases

BRENDA2.7.11.22. 1939.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP54685.

Entry information

Entry nameCDK7_DICDI
AccessionPrimary (citable) accession number: P54685
Secondary accession number(s): Q54MR4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase