Reviewed, UniProtKB/Swiss-Prot P54685 (CDK7_DICDI)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 7
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    CDK-activating kinase
      Short name=CAK
    MO15 homolog

Gene names

Name:	cdk7
Synonyms:	cdcC, cdcD, mo15
ORF Names:	DDB_G0285417

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalytic part of CAK which activates cyclin-associated CDC2/CDK2/CDK4 by threonine phosphorylation, thereby allowing MPF activation By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subunit structure	Probably associates with cyclin H and mat1 to form a multimeric active enzyme By similarity.
Subcellular location	Nucleus By similarity.
Developmental stage	Only expressed during vegetative cell growth.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Cell division protein kinase 7		PRO_0000085796
Regions
	Domain	4 – 288	285	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
	Compositional bias	326 – 342	17	Poly-Gln
Sites
	Active site	130	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	157	1	Phosphoserine By similarity
	Modified residue	163	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	360	1	V → VKK in AAB35208. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54685-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: F6FE0441F21D6B37	FASTA	360	40,567
	10 20 30 40 50 60 MDKYNIEALI GEGTYGVVSR ATVKATGQIV AIKKIRKILI QNQTDDGINF SAIREIKILQ 70 80 90 100 110 120 ELKHDNVVNL LDIFAHKSNV YLVFELMQWD LQEVIEDKSI ILKPADIKSY MKMLLQGIEA 130 140 150 160 170 180 CHRNWVLHRD LKPNNLLMSI NGDLKLADFG LARQYGSPNK VFSPQAVTIF YRAPELLFGA 190 200 210 220 230 240 KSYGPSVDIW SIGCIFAELM LRTPYLPGTG EIDQLRKICS ALGTPNESNW PGVTCLPNYI 250 260 270 280 290 300 KFTDHPATPF KQLFTAASDE AIDLISKMLL FNPSNRISAA DALNHPYFTS GVKHTNPADL 310 320 330 340 350 360 PVPFAKKASL LQQRQVLAQV QQQLLQKQQQ QQQQQQQQIQ SQPEPIQVDN VEQTQQAQQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Dictyostelium discoideum gene, which is highly related to mo15 from Xenopus, is expressed during growth but not during development." Michaelis C.E., Luo Q., Weeks G. Biochem. Cell Biol. 73:51-58(1995) [PubMed: 7662315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: V12M2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S79590 mRNA. Translation: AAB35208.2. AAFI02000079 Genomic DNA. Translation: EAL64546.1.
RefSeq	XP_638229.1.
3D structure databases
HSSP	HSSP built from PDB template 1H00 based on UniProtKB P24941.
ModBase	Search...
Genome annotation databases
GeneID	3389717.
GenomeReviews	Gene locus cdk7 in contig CM000153_GR.
KEGG	ddi:DDB_0191429.
Organism-specific databases
dictyBase	DDB_G0285417. cdk7.
Phylogenomic databases
OMA	FMDTDLE.
Enzyme and pathway databases
BRENDA	2.7.11.22. 424. 2.7.11.23. 424.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK7_DICDI

Accession

Primary (citable) accession number: P54685
Secondary accession number(s): Q54MR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)