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Protein

Serine protease/ABC transporter B family protein tagB

Gene

tagB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Intercellular communication via tagB may mediate integration of cellular differentiation with morphogenesis.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei387Charge relay systemBy similarity1
Active sitei432Charge relay systemBy similarity1
Active sitei695Charge relay systemBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1553 – 1560ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: dictyBase
  • ATP binding Source: UniProtKB-KW
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: dictyBase
  • signal transducer activity Source: dictyBase

GO - Biological processi

  • proteolysis Source: dictyBase
  • signal transduction Source: dictyBase
  • sorocarp development Source: dictyBase
  • sorocarp stalk cell differentiation Source: dictyBase
  • transport Source: dictyBase

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processTransport
LigandATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiS08.A57.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease/ABC transporter B family protein tagB (EC:3.4.21.-)
Alternative name(s):
Prestalk-specific protein tagB
Gene namesi
Name:tagB
ORF Names:DDB_G0286119
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0286119. tagB.

Subcellular locationi

P54683:
  • Membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1011 – 1031HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1076 – 1096HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1121 – 1141HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1210 – 1230HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1309 – 1329HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1332 – 1352HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: dictyBase

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Null mutations in either tagB or tagC lead to a cell autonomous defect in prestalk differentiation and a 5-fold reduction in the expression of the prestalk gene ecmA. Disruption of regA in a tagB null or in a tagC null background results in higher levels of sporulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000002719132 – 1906Serine protease/ABC transporter B family protein tagBAdd BLAST1875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi594N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi621N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi672N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi747N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi823N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1172N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1522N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1658N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP54683.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0191217.

Structurei

3D structure databases

ProteinModelPortaliP54683.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini360 – 758Peptidase S8Add BLAST399
Domaini1080 – 1363ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST284
Domaini1518 – 1756ABC transporterPROSITE-ProRule annotationAdd BLAST239

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi63 – 67Poly-Gln5
Compositional biasi95 – 104Poly-Asn10
Compositional biasi107 – 134Poly-AsnAdd BLAST28
Compositional biasi311 – 321Poly-SerAdd BLAST11
Compositional biasi833 – 837Poly-Ser5
Compositional biasi838 – 844Poly-Gly7
Compositional biasi871 – 876Poly-Leu6
Compositional biasi1012 – 1015Poly-Ile4
Compositional biasi1386 – 1389Poly-Glu4
Compositional biasi1398 – 1404Poly-Gly7
Compositional biasi1445 – 1450Poly-Asn6
Compositional biasi1765 – 1779Poly-AsnAdd BLAST15
Compositional biasi1782 – 1785Poly-Ser4
Compositional biasi1807 – 1812Poly-Pro6
Compositional biasi1813 – 1861Poly-GlnAdd BLAST49
Compositional biasi1873 – 1879Poly-Pro7

Sequence similaritiesi

In the N-terminal section; belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410INIQ. Eukaryota.
KOG0058. Eukaryota.
COG1132. LUCA.
COG2274. LUCA.
InParanoidiP54683.
OMAiWISWIGE.
PhylomeDBiP54683.

Family and domain databases

CDDicd04842. Peptidases_S8_Kp43_protease. 1 hit.
Gene3Di1.20.1560.10. 1 hit.
1.25.10.10. 1 hit.
3.40.50.200. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR036640. ABC1_TM_sf.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011989. ARM-like.
IPR008979. Galactose-bd-like.
IPR027417. P-loop_NTPase.
IPR000209. Peptidase_S8/S53_dom.
IPR036852. Peptidase_S8/S53_dom_sf.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR034058. TagA/B/C/D_pept_dom.
PfamiView protein in Pfam
PF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PRINTSiPR00723. SUBTILISIN.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF52743. SSF52743. 3 hits.
SSF90123. SSF90123. 1 hit.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFQFSSPSK IFLFSSVILI LIFIGIKFEL LEDTNSNRND KFNNIINRFI
60 70 80 90 100
NYNIDDSIYK NKQQQQQFSN KIYSNEKKIL LKNKIIDTTI KPSININNNN
110 120 130 140 150
NNNNKLNNNN NNNNNNNNNN NNNNNNNNNN NNNNYYNSIE YYSSFVSRLL
160 170 180 190 200
KSNDDDGIYY DDYQSKYKKN HYIVQFKDRI NDETREQLKE FLIGTDITIL
210 220 230 240 250
KEQPFKSHIV HYIPHDSFLV FMTKEQSVLL SSKEWISWIG EHEPSNKIHL
260 270 280 290 300
NYHEKSIGYP VYIILSGSTN SLIQRWENTL NSILTSYNSK VKLTLINQKK
310 320 330 340 350
LKSIVYCNDE SPSSSSSSSC SLIGSEKIVY KWISEQSESN YIERSEKLQT
360 370 380 390 400
ANRLSPTVIF GTKDKLVNND RIDIPLRGKG QILSIADTGL DGSHCFFSDS
410 420 430 440 450
KYPIPFNQVN ENHRKVVTYI TYHDNEDYVN GHGTHVCGSA AGTPEDSSWA
460 470 480 490 500
ISSFSGLATD AKIAFYDLSS GSSEPTPPED YSQMYKPLYD AGARVHGDSW
510 520 530 540 550
GSVSLQGYYG GYSDDAGGID AFLYEYPEFS ILRAAGNNEL FASLLAQATA
560 570 580 590 600
KNAITVGAEQ TAHVNYVSDA LEYYDFSDNA NFQRPCLFDK KYCNYTTAKC
610 620 630 640 650
CSEVSNVKGL QLCCPASIKQ NASDSFTTQP QFYNENNMGS FSSKGPTHDG
660 670 680 690 700
RLKPDIVAPG EYITSARSNG ENSTDQCGDG SLPNANGLMS ISGTSMATPL
710 720 730 740 750
ATAATTILRQ YLVDGYFPTG ESVEENKLLP TGSLLKALMI NNAQLLNGTY
760 770 780 790 800
FWSASSTNPS NAIFEQINGA NLIQGWGALR MNNWLYVKSS NPTPPSRWIG
810 820 830 840 850
IGGLGKNQKA TEWKEDSLSS GLNKSYCFTY KPSSSSSGSG GGGGTPRIVA
860 870 880 890 900
TLVWTDPPSY SGAKFNLVNN LDLLLLNSDD DSIITIGNSG GSLQPAGKVA
910 920 930 940 950
QPDTLNNVEG IIINPTKAMN YKFTIAGTNV PIGPQKFSFV FHGENGQFDW
960 970 980 990 1000
ADSCPQCVDG VQFPCLITNG IGIQSCGSDL LWTKCIVQSC NEGYNYNSLK
1010 1020 1030 1040 1050
NTCDKFLSYN YIIIIVAGGT MVLIILLLMW IKYQEYKESK RDSFRRFDDG
1060 1070 1080 1090 1100
TGIFVRPKDK DAKVTPPDLY NLVSPFIIEL TIATACSLVA TAASILQPFY
1110 1120 1130 1140 1150
IGNIVNNIPT TKSIGEFKSD FIIIFILAFI EFLFTNVGSW ISGIVNEKMV
1160 1170 1180 1190 1200
MRLQNKVFRA LIAQDMGFFQ RNNSALLMNV LIVDTPMLRS ALTGILLSIA
1210 1220 1230 1240 1250
TGVCKLVGSL VFIFTISWKL SLAFFAAVPI LGLVTQIQSQ FTKRLTRQLL
1260 1270 1280 1290 1300
FHNSKASQHG TESLTNMHVV TNYCKQEKEM VKYSDQLMNV FITARKLIIT
1310 1320 1330 1340 1350
NTFAGTGKWL LIESLTFVIL YFSAYLVIQK QFTVGLMISF SLYIGYVVDA
1360 1370 1380 1390 1400
SSSLFGVYVS YIQCLASATR VFMILRSAPR KRTTLEEEEA DRLAGLSGGG
1410 1420 1430 1440 1450
GGGGDNGDDK KDKQNIENGK DVLPSNIITP IDNVENSNGK QDDPNNNNNN
1460 1470 1480 1490 1500
IGNLDYSEQL DGVSTVADST VGLTKRELKK QKEKEQKEYF KQTGISVAET
1510 1520 1530 1540 1550
PTFLPSSYTE LTECRGEIEF KNVSFRYPTR PDVQVLHNIN MKFEAGKCYG
1560 1570 1580 1590 1600
LVGPSGSGKS TTLELISKFY PLHGETGGKI YMDGIDIAKI RPNNLRSFVT
1610 1620 1630 1640 1650
NVHQHPFLFD ATIGENIGYA IDNPTQEDII EAAKLAYAHE FINDLPKKYD
1660 1670 1680 1690 1700
TQIGEAGNLS GGQKKRIAVA RAICAKRKIM LLDEITAELD PESEEAITQS
1710 1720 1730 1740 1750
IKVLTQGHTV VMVAHKVAAV RDCDKIFVLE KGYLVEEGTH DELMANKGKY
1760 1770 1780 1790 1800
YRMFSEDKDD TPLQNNNNNK NNNNNNNNNE PSSSSTPPND QPTPPPQEQQ
1810 1820 1830 1840 1850
EQKNDQPPPP PPQEQQEQQE QQQQQQQEQQ QQQQQQQQQQ QQQQQQQQQQ
1860 1870 1880 1890 1900
QQQQQQQQQQ QNDQPPNDYD QVPPPPPLPS ESPSPPTGNN DGQPLVQMDE

ENDEER
Length:1,906
Mass (Da):212,647
Last modified:December 4, 2007 - v2
Checksum:iCCAAD3D6857CED20
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1829Missing in AAA62212 (PubMed:7744252).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20432 Genomic DNA. Translation: AAA62212.1.
AAFI02000085 Genomic DNA. Translation: EAL64352.1.
PIRiT18267.
RefSeqiXP_637865.1. XM_632773.1.

Genome annotation databases

EnsemblProtistsiEAL64352; EAL64352; DDB_G0286119.
GeneIDi8625462.
KEGGiddi:DDB_G0286119.

Similar proteinsi

Entry informationi

Entry nameiTAGB_DICDI
AccessioniPrimary (citable) accession number: P54683
Secondary accession number(s): Q54M83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: October 25, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families