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Protein

Probable plasma membrane ATPase

Gene

patB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The plasma membrane ATPase is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+ symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei480 – 48014-aspartylphosphate intermediateBy similarity
Metal bindingi728 – 7281MagnesiumBy similarity
Metal bindingi732 – 7321MagnesiumBy similarity

GO - Molecular functioni

  • ATPase activity Source: dictyBase
  • ATP binding Source: dictyBase
  • hydrogen-exporting ATPase activity, phosphorylative mechanism Source: dictyBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ATP biosynthetic process Source: InterPro
  • hydrogen ion transmembrane transport Source: GOC
  • proton transport Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-418359. Reduction of cytosolic Ca++ levels.
R-DDI-5578775. Ion homeostasis.
R-DDI-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable plasma membrane ATPase (EC:3.6.3.6)
Alternative name(s):
PAT2
Proton pump
Gene namesi
Name:patB
ORF Names:DDB_G0282817
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0282817. patB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 212212CytoplasmicSequence analysisAdd
BLAST
Transmembranei213 – 23220HelicalSequence analysisAdd
BLAST
Topological domaini233 – 2375ExtracellularSequence analysis
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Topological domaini259 – 387129CytoplasmicSequence analysisAdd
BLAST
Transmembranei388 – 40720HelicalSequence analysisAdd
BLAST
Topological domaini408 – 42518ExtracellularSequence analysisAdd
BLAST
Transmembranei426 – 44722HelicalSequence analysisAdd
BLAST
Topological domaini448 – 783336CytoplasmicSequence analysisAdd
BLAST
Transmembranei784 – 80522HelicalSequence analysisAdd
BLAST
Topological domaini806 – 8105ExtracellularSequence analysis
Transmembranei811 – 83323HelicalSequence analysisAdd
BLAST
Topological domaini834 – 84916CytoplasmicSequence analysisAdd
BLAST
Transmembranei850 – 87021HelicalSequence analysisAdd
BLAST
Topological domaini871 – 88919ExtracellularSequence analysisAdd
BLAST
Transmembranei890 – 91021HelicalSequence analysisAdd
BLAST
Topological domaini911 – 92212CytoplasmicSequence analysisAdd
BLAST
Transmembranei923 – 94321HelicalSequence analysisAdd
BLAST
Topological domaini944 – 96724ExtracellularSequence analysisAdd
BLAST
Transmembranei968 – 98821HelicalSequence analysisAdd
BLAST
Topological domaini989 – 105870CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: dictyBase
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10581058Probable plasma membrane ATPasePRO_0000046295Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54679.
PRIDEiP54679.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0214946.

Structurei

3D structure databases

ProteinModelPortaliP54679.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi44 – 5512Poly-GlnAdd
BLAST
Compositional biasi113 – 1164Poly-Ser
Compositional biasi246 – 2494Poly-Leu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
InParanoidiP54679.
KOiK01535.
OMAiQPPEASN.
PhylomeDBiP54679.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNNQIPKNS PESSAINSAE SSPKSNVSSD VLHENHHKEQ QQLQQQLQQE
60 70 80 90 100
QQQQQLPTTP QSEPTQRVNN NEEGIEMDRI AENSNINVPS DVGESSLKTI
110 120 130 140 150
SGYPSSKNTE AGSSSSGKKE EDYNYRSTWV PKLHQELFEN PEVLESRRTK
160 170 180 190 200
QRASNYRKTL EREKEGIKPL DNILEELKAN ANGLTKAEAQ KRLEEVGPNA
210 220 230 240 250
IPDVKRYPIL EFLYFMWNPL SWTMEVAAIV SIALLDWVDF ILICALLLLN
260 270 280 290 300
ATIGFIEENT AGNAVEALKN SLVSQIRCMR DGEWVMLPSP DLVPGDVVML
310 320 330 340 350
KIGAIIPADC RVIEAEQVKI DQSSLTGESL PVTKKIGDEV YSGSAMKQGE
360 370 380 390 400
AKCVVTATGV NTFFGRAANL VQETEGHGHL QVILRNIGLF CISFIAIWVL
410 420 430 440 450
VELLVDFLGY DGYCHGVGGG RCLPLNNALV LLVGGIPIAM PTVLSVTMAI
460 470 480 490 500
GATQLSKKKA IVSRLASIEE LAAMDILCSD KTGTLTLNIL TVDEPLPVGD
510 520 530 540 550
TPKEDIVFHA FLACSEGEDQ DAIDKAISNY CRDTYPNVDY SGNEIVKHYP
560 570 580 590 600
FNPEDKKAMG LVNANGKQFK TAKGAPQIIL READNYKQVG EAVEKEIENL
610 620 630 640 650
ADRGYRALGV SVSYDAPDFK VWHFEGLIPL FDPPRHDTED TIKRALEMGV
660 670 680 690 700
SVKMITGDQL AIAKETARRL GMGGNLFTIP YLENNDLGIS EGEVIEMADG
710 720 730 740 750
FAEMWPEHKY KVVDQLQKRK HVVGMTGDGV NDAPALKKAQ IGIAVAGATD
760 770 780 790 800
AARSVSDIVL TSSGLSVIID AIISSRKIFQ RMRNYVIYSV AATVRICTTF
810 820 830 840 850
GILTVAWNFK FPTIATVIIA ILNDGTMLTI SKDRVRARNE PDQWNLFEVF
860 870 880 890 900
TMALCYGFYL VGSTIVFFAI IHDGTWFHDA INLRILTDNE LRGLIYLQVS
910 920 930 940 950
ISGLATIFVS RSQGFSYFER PGNLVIFAFV MSQIVATFIG VYGFRGYPHD
960 970 980 990 1000
SFSDNPDYPV HGTNFQGCGW GWAVCAWIWC FLWYIPMDFI KLGVTYILRG
1010 1020 1030 1040 1050
KIEPINKDAL RKIYGWFGKE IPKEATQVSH KVAEQQAKRD ALHAQETHHK

SVVTDNKV
Length:1,058
Mass (Da):117,268
Last modified:December 4, 2007 - v2
Checksum:i12D39896E92F3A9F
GO

Sequence cautioni

The sequence CAA66931.1 differs from that shown. Reason: Frameshift at positions 517 and 561. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98286 mRNA. Translation: CAA66931.1. Frameshift.
AAFI02000047 Genomic DNA. Translation: EAL65988.1.
PIRiT30580.
RefSeqiXP_639363.1. XM_634271.1.

Genome annotation databases

EnsemblProtistsiDDB0214946; DDB0214946; DDB_G0282817.
GeneIDi8623803.
KEGGiddi:DDB_G0282817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98286 mRNA. Translation: CAA66931.1. Frameshift.
AAFI02000047 Genomic DNA. Translation: EAL65988.1.
PIRiT30580.
RefSeqiXP_639363.1. XM_634271.1.

3D structure databases

ProteinModelPortaliP54679.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0214946.

Proteomic databases

PaxDbiP54679.
PRIDEiP54679.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0214946; DDB0214946; DDB_G0282817.
GeneIDi8623803.
KEGGiddi:DDB_G0282817.

Organism-specific databases

dictyBaseiDDB_G0282817. patB.

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
InParanoidiP54679.
KOiK01535.
OMAiQPPEASN.
PhylomeDBiP54679.

Enzyme and pathway databases

ReactomeiR-DDI-418359. Reduction of cytosolic Ca++ levels.
R-DDI-5578775. Ion homeostasis.
R-DDI-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP54679.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The patB gene of Dictyostelium discoideum encodes a P-type H(+)-ATPase isoform essential for growth and development under acidic conditions."
    Coukell M.B., Moniakis J., Cameron A.M.
    Microbiology 143:3877-3888(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPMA1_DICDI
AccessioniPrimary (citable) accession number: P54679
Secondary accession number(s): Q54RX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: May 11, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.