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P54679 (PMA1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable plasma membrane ATPase
EC=3.6.3.6
Alternative name(s):
PAT2
Proton pump
Gene names
Name:patB
ORF Names:DDB_G0282817
OrganismDictyostelium discoideum (Slime mold)
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length1058 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The plasma membrane ATPase is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+ symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activity

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]

Sequence caution

The sequence CAA66931.1 differs from that shown. Reason: Frameshift at positions 517 and 561.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10581058Probable plasma membrane ATPase
PRO_0000046295

Regions

Topological domain1 – 212212Cytoplasmic Potential
Transmembrane213 – 23220Helical; Potential
Topological domain233 – 2375Extracellular Potential
Transmembrane238 – 25821Helical; Potential
Topological domain259 – 387129Cytoplasmic Potential
Transmembrane388 – 40720Helical; Potential
Topological domain408 – 42518Extracellular Potential
Transmembrane426 – 44722Helical; Potential
Topological domain448 – 783336Cytoplasmic Potential
Transmembrane784 – 80522Helical; Potential
Topological domain806 – 8105Extracellular Potential
Transmembrane811 – 83323Helical; Potential
Topological domain834 – 84916Cytoplasmic Potential
Transmembrane850 – 87021Helical; Potential
Topological domain871 – 88919Extracellular Potential
Transmembrane890 – 91021Helical; Potential
Topological domain911 – 92212Cytoplasmic Potential
Transmembrane923 – 94321Helical; Potential
Topological domain944 – 96724Extracellular Potential
Transmembrane968 – 98821Helical; Potential
Topological domain989 – 105870Cytoplasmic Potential
Compositional bias44 – 5512Poly-Gln
Compositional bias113 – 1164Poly-Ser
Compositional bias246 – 2494Poly-Leu

Sites

Active site48014-aspartylphosphate intermediate By similarity
Metal binding7281Magnesium By similarity
Metal binding7321Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P54679 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 12D39896E92F3A9F

FASTA1,058117,268
        10         20         30         40         50         60 
MDNNQIPKNS PESSAINSAE SSPKSNVSSD VLHENHHKEQ QQLQQQLQQE QQQQQLPTTP 

        70         80         90        100        110        120 
QSEPTQRVNN NEEGIEMDRI AENSNINVPS DVGESSLKTI SGYPSSKNTE AGSSSSGKKE 

       130        140        150        160        170        180 
EDYNYRSTWV PKLHQELFEN PEVLESRRTK QRASNYRKTL EREKEGIKPL DNILEELKAN 

       190        200        210        220        230        240 
ANGLTKAEAQ KRLEEVGPNA IPDVKRYPIL EFLYFMWNPL SWTMEVAAIV SIALLDWVDF 

       250        260        270        280        290        300 
ILICALLLLN ATIGFIEENT AGNAVEALKN SLVSQIRCMR DGEWVMLPSP DLVPGDVVML 

       310        320        330        340        350        360 
KIGAIIPADC RVIEAEQVKI DQSSLTGESL PVTKKIGDEV YSGSAMKQGE AKCVVTATGV 

       370        380        390        400        410        420 
NTFFGRAANL VQETEGHGHL QVILRNIGLF CISFIAIWVL VELLVDFLGY DGYCHGVGGG 

       430        440        450        460        470        480 
RCLPLNNALV LLVGGIPIAM PTVLSVTMAI GATQLSKKKA IVSRLASIEE LAAMDILCSD 

       490        500        510        520        530        540 
KTGTLTLNIL TVDEPLPVGD TPKEDIVFHA FLACSEGEDQ DAIDKAISNY CRDTYPNVDY 

       550        560        570        580        590        600 
SGNEIVKHYP FNPEDKKAMG LVNANGKQFK TAKGAPQIIL READNYKQVG EAVEKEIENL 

       610        620        630        640        650        660 
ADRGYRALGV SVSYDAPDFK VWHFEGLIPL FDPPRHDTED TIKRALEMGV SVKMITGDQL 

       670        680        690        700        710        720 
AIAKETARRL GMGGNLFTIP YLENNDLGIS EGEVIEMADG FAEMWPEHKY KVVDQLQKRK 

       730        740        750        760        770        780 
HVVGMTGDGV NDAPALKKAQ IGIAVAGATD AARSVSDIVL TSSGLSVIID AIISSRKIFQ 

       790        800        810        820        830        840 
RMRNYVIYSV AATVRICTTF GILTVAWNFK FPTIATVIIA ILNDGTMLTI SKDRVRARNE 

       850        860        870        880        890        900 
PDQWNLFEVF TMALCYGFYL VGSTIVFFAI IHDGTWFHDA INLRILTDNE LRGLIYLQVS 

       910        920        930        940        950        960 
ISGLATIFVS RSQGFSYFER PGNLVIFAFV MSQIVATFIG VYGFRGYPHD SFSDNPDYPV 

       970        980        990       1000       1010       1020 
HGTNFQGCGW GWAVCAWIWC FLWYIPMDFI KLGVTYILRG KIEPINKDAL RKIYGWFGKE 

      1030       1040       1050 
IPKEATQVSH KVAEQQAKRD ALHAQETHHK SVVTDNKV

« Hide

References

« Hide 'large scale' references
[1]"The patB gene of Dictyostelium discoideum encodes a P-type H(+)-ATPase isoform essential for growth and development under acidic conditions."
Coukell M.B., Moniakis J., Cameron A.M.
Microbiology 143:3877-3888(1997) [PubMed: 9421912] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed: 15875012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X98286 mRNA. Translation: CAA66931.1. Frameshift.
AAFI02000047 Genomic DNA. Translation: EAL65988.1.
PIRT30580.
RefSeqXP_639363.1. XM_634271.1.

3D structure databases

ProteinModelPortalP54679.
ModBaseSearch...

Protein-protein interaction databases

STRINGP54679.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0214946; DDB0214946; DDB_G0282817.
GeneID8623803.
GenomeReviewsGene locus patB in contig CM000152_GR.
KEGGddi:DDB_G0282817.

Organism-specific databases

dictyBaseDDB_G0282817. patB.

Phylogenomic databases

eggNOGKOG0205.
GeneTreeEPrGT00050000000458.
HOGENOMHBG706356.
OMADNNDELM.
PhylomeDBP54679.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR006534. ATPase_P-typ_PM_proton-efflux.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 3 hits.
KOK01535.
PANTHERPTHR24093:SF61. PTHR24093:SF61. 1 hit.
PfamPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMA1_DICDI
AccessionPrimary (citable) accession number: P54679
Secondary accession number(s): Q54RX4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: November 16, 2011
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents