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Protein

Probable plasma membrane ATPase

Gene

patB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The plasma membrane ATPase is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+ symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4804-aspartylphosphate intermediateBy similarity1
Metal bindingi728MagnesiumBy similarity1
Metal bindingi732MagnesiumBy similarity1

GO - Molecular functioni

  • ATPase activity Source: dictyBase
  • ATP binding Source: dictyBase
  • hydrogen-exporting ATPase activity, phosphorylative mechanism Source: dictyBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ATP biosynthetic process Source: InterPro
  • proton transport Source: dictyBase
  • regulation of intracellular pH Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-418359. Reduction of cytosolic Ca++ levels.
R-DDI-5578775. Ion homeostasis.
R-DDI-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable plasma membrane ATPase (EC:3.6.3.6)
Alternative name(s):
PAT2
Proton pump
Gene namesi
Name:patB
ORF Names:DDB_G0282817
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0282817. patB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 212CytoplasmicSequence analysisAdd BLAST212
Transmembranei213 – 232HelicalSequence analysisAdd BLAST20
Topological domaini233 – 237ExtracellularSequence analysis5
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 387CytoplasmicSequence analysisAdd BLAST129
Transmembranei388 – 407HelicalSequence analysisAdd BLAST20
Topological domaini408 – 425ExtracellularSequence analysisAdd BLAST18
Transmembranei426 – 447HelicalSequence analysisAdd BLAST22
Topological domaini448 – 783CytoplasmicSequence analysisAdd BLAST336
Transmembranei784 – 805HelicalSequence analysisAdd BLAST22
Topological domaini806 – 810ExtracellularSequence analysis5
Transmembranei811 – 833HelicalSequence analysisAdd BLAST23
Topological domaini834 – 849CytoplasmicSequence analysisAdd BLAST16
Transmembranei850 – 870HelicalSequence analysisAdd BLAST21
Topological domaini871 – 889ExtracellularSequence analysisAdd BLAST19
Transmembranei890 – 910HelicalSequence analysisAdd BLAST21
Topological domaini911 – 922CytoplasmicSequence analysisAdd BLAST12
Transmembranei923 – 943HelicalSequence analysisAdd BLAST21
Topological domaini944 – 967ExtracellularSequence analysisAdd BLAST24
Transmembranei968 – 988HelicalSequence analysisAdd BLAST21
Topological domaini989 – 1058CytoplasmicSequence analysisAdd BLAST70

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462951 – 1058Probable plasma membrane ATPaseAdd BLAST1058

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54679.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0214946.

Structurei

3D structure databases

ProteinModelPortaliP54679.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi44 – 55Poly-GlnAdd BLAST12
Compositional biasi113 – 116Poly-Ser4
Compositional biasi246 – 249Poly-Leu4

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
InParanoidiP54679.
KOiK01535.
OMAiQEMHFLP.
PhylomeDBiP54679.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54679-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNNQIPKNS PESSAINSAE SSPKSNVSSD VLHENHHKEQ QQLQQQLQQE
60 70 80 90 100
QQQQQLPTTP QSEPTQRVNN NEEGIEMDRI AENSNINVPS DVGESSLKTI
110 120 130 140 150
SGYPSSKNTE AGSSSSGKKE EDYNYRSTWV PKLHQELFEN PEVLESRRTK
160 170 180 190 200
QRASNYRKTL EREKEGIKPL DNILEELKAN ANGLTKAEAQ KRLEEVGPNA
210 220 230 240 250
IPDVKRYPIL EFLYFMWNPL SWTMEVAAIV SIALLDWVDF ILICALLLLN
260 270 280 290 300
ATIGFIEENT AGNAVEALKN SLVSQIRCMR DGEWVMLPSP DLVPGDVVML
310 320 330 340 350
KIGAIIPADC RVIEAEQVKI DQSSLTGESL PVTKKIGDEV YSGSAMKQGE
360 370 380 390 400
AKCVVTATGV NTFFGRAANL VQETEGHGHL QVILRNIGLF CISFIAIWVL
410 420 430 440 450
VELLVDFLGY DGYCHGVGGG RCLPLNNALV LLVGGIPIAM PTVLSVTMAI
460 470 480 490 500
GATQLSKKKA IVSRLASIEE LAAMDILCSD KTGTLTLNIL TVDEPLPVGD
510 520 530 540 550
TPKEDIVFHA FLACSEGEDQ DAIDKAISNY CRDTYPNVDY SGNEIVKHYP
560 570 580 590 600
FNPEDKKAMG LVNANGKQFK TAKGAPQIIL READNYKQVG EAVEKEIENL
610 620 630 640 650
ADRGYRALGV SVSYDAPDFK VWHFEGLIPL FDPPRHDTED TIKRALEMGV
660 670 680 690 700
SVKMITGDQL AIAKETARRL GMGGNLFTIP YLENNDLGIS EGEVIEMADG
710 720 730 740 750
FAEMWPEHKY KVVDQLQKRK HVVGMTGDGV NDAPALKKAQ IGIAVAGATD
760 770 780 790 800
AARSVSDIVL TSSGLSVIID AIISSRKIFQ RMRNYVIYSV AATVRICTTF
810 820 830 840 850
GILTVAWNFK FPTIATVIIA ILNDGTMLTI SKDRVRARNE PDQWNLFEVF
860 870 880 890 900
TMALCYGFYL VGSTIVFFAI IHDGTWFHDA INLRILTDNE LRGLIYLQVS
910 920 930 940 950
ISGLATIFVS RSQGFSYFER PGNLVIFAFV MSQIVATFIG VYGFRGYPHD
960 970 980 990 1000
SFSDNPDYPV HGTNFQGCGW GWAVCAWIWC FLWYIPMDFI KLGVTYILRG
1010 1020 1030 1040 1050
KIEPINKDAL RKIYGWFGKE IPKEATQVSH KVAEQQAKRD ALHAQETHHK

SVVTDNKV
Length:1,058
Mass (Da):117,268
Last modified:December 4, 2007 - v2
Checksum:i12D39896E92F3A9F
GO

Sequence cautioni

The sequence CAA66931 differs from that shown. Reason: Frameshift at positions 517 and 561.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98286 mRNA. Translation: CAA66931.1. Frameshift.
AAFI02000047 Genomic DNA. Translation: EAL65988.1.
PIRiT30580.
RefSeqiXP_639363.1. XM_634271.1.

Genome annotation databases

EnsemblProtistsiEAL65988; EAL65988; DDB_G0282817.
GeneIDi8623803.
KEGGiddi:DDB_G0282817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98286 mRNA. Translation: CAA66931.1. Frameshift.
AAFI02000047 Genomic DNA. Translation: EAL65988.1.
PIRiT30580.
RefSeqiXP_639363.1. XM_634271.1.

3D structure databases

ProteinModelPortaliP54679.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0214946.

Proteomic databases

PaxDbiP54679.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL65988; EAL65988; DDB_G0282817.
GeneIDi8623803.
KEGGiddi:DDB_G0282817.

Organism-specific databases

dictyBaseiDDB_G0282817. patB.

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
InParanoidiP54679.
KOiK01535.
OMAiQEMHFLP.
PhylomeDBiP54679.

Enzyme and pathway databases

ReactomeiR-DDI-418359. Reduction of cytosolic Ca++ levels.
R-DDI-5578775. Ion homeostasis.
R-DDI-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP54679.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 2 hits.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMA1_DICDI
AccessioniPrimary (citable) accession number: P54679
Secondary accession number(s): Q54RX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.