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Protein

Calcium-transporting ATPase PAT1

Gene

patA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Calcium ATPase involved in Ca2+ homeostasis as a component of the contractile vacuole complex.2 Publications

Miscellaneous

Loss-of-function mutant (antisense inhibition) displays impaired growth in high Ca2+ medium but normal growth in low Ca2+ medium. Antisense inhibition does not affect development in high Ca2+ medium.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3854-aspartylphosphate intermediateBy similarity1
Metal bindingi678MagnesiumBy similarity1
Metal bindingi682MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: dictyBase
  • calcium ion binding Source: dictyBase
  • calcium ion transmembrane transporter activity Source: dictyBase
  • calcium-transporting ATPase activity Source: UniProtKB

GO - Biological processi

  • calcium ion transport Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-418359 Reduction of cytosolic Ca++ levels
R-DDI-5578775 Ion homeostasis
R-DDI-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBi3.A.3.2.17 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase PAT1 (EC:3.6.3.8)
Gene namesi
Name:patA
ORF Names:DDB_G0277861
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0277861 patA

Subcellular locationi

  • Contractile vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication
  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
  • Note: Contractile vacuole complex. Localizes to the contractile vacuole membrane in unstimulated cells (PubMed:9885293). Localizes to the cell membrane and the contractile vacuole membrane in cells stimulated by calcium (PubMed:9885293).1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 99StromalSequence analysisAdd BLAST99
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121 – 126LumenalSequence analysis6
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Topological domaini148 – 235StromalSequence analysisAdd BLAST88
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Topological domaini257 – 287LumenalSequence analysisAdd BLAST31
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Topological domaini309 – 328StromalSequence analysisAdd BLAST20
Transmembranei329 – 349HelicalSequence analysisAdd BLAST21
Topological domaini350 – 735LumenalSequence analysisAdd BLAST386
Transmembranei736 – 756HelicalSequence analysisAdd BLAST21
Topological domaini757 – 832StromalSequence analysisAdd BLAST76
Transmembranei833 – 853HelicalSequence analysisAdd BLAST21
Topological domaini854 – 873LumenalSequence analysisAdd BLAST20
Transmembranei874 – 894HelicalSequence analysisAdd BLAST21
Topological domaini895 – 913StromalSequence analysisAdd BLAST19
Transmembranei914 – 934HelicalSequence analysisAdd BLAST21
Topological domaini935 – 943LumenalSequence analysis9
Transmembranei944 – 964HelicalSequence analysisAdd BLAST21
Topological domaini965 – 1115StromalSequence analysisAdd BLAST151

GO - Cellular componenti

  • contractile vacuolar membrane Source: UniProtKB
  • integral component of membrane Source: dictyBase
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: UniProtKB

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000461791 – 1115Calcium-transporting ATPase PAT1Add BLAST1115

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54678
PRIDEiP54678

Expressioni

Developmental stagei

Expressed constitutively at very low levels during vegetative growth and throughout development.1 Publication

Inductioni

By calcium.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0214945

Structurei

3D structure databases

ProteinModelPortaliP54678
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
InParanoidiP54678
KOiK01537
OMAiTHIRVVN
PhylomeDBiP54678

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 3 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

P54678-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGSHEMESI MLDSMEEEFP VSVETLGKLV DVPKGFDTYA ELGGLSGLST
60 70 80 90 100
KLKSNIKTGL PLEKSSTEEN RVLKYSKNIL PDPPHQPLWS IVLDALSDHI
110 120 130 140 150
LILLIVAAVV SIVLGSIDYT SDHPETGWID GVAILVAVIL VVGITSLNDF
160 170 180 190 200
KNQARFRELN DKSNDKEVKG IRGGEQCQIS IFDVKVGDII SLDTGDIICA
210 220 230 240 250
DGVFIEGHAL KCDESSITGE SDPIKKGQPQ DNMDPFLISG SMVIEGFGTM
260 270 280 290 300
LVTAVGVNSF NGKTMMGLRV ASEDTPLQMK LSVLASRIGY FGMGAAILML
310 320 330 340 350
LIAIPKYFIQ RKVHDIEITR EDAQPIVQLV ISAITIVVVA VPEGLPLAVT
360 370 380 390 400
MALAYGMMKM FKENNLVRNL ASCETMGSAT TICSDKTGTL TQNVMSVVTG
410 420 430 440 450
TICGVFPTLD GIAQKIPKHV QSILTDGMAI NSNAYEGVSS KGKLEFIGSK
460 470 480 490 500
TECALLNFGK LFGCDYNEVR KRLEVVELYP FSSARKRMSV LVKHDQNLRL
510 520 530 540 550
FTKGASEIIL GQCGSYLDEA GNIRPISEAK AYFEEQINNF ASDALRTIGL
560 570 580 590 600
AYRDFQYGEC DFKEPPENNL VFIGIVGIKD PLRPEVPEAV EICKRAGIVV
610 620 630 640 650
RMVTGDNLVT AQNIARNCGI LTEGGLCMEG PKFRELSQSE MDAILPKLQV
660 670 680 690 700
LARSSPTDKQ LLVGRLKDLG EVVAVTGDGT NDGPALKLAN VGFSMGISGT
710 720 730 740 750
EVAIAASDVV LLDDNFASIV RAVLWGRNIY DAICKFLQFQ LTVNVVAVTV
760 770 780 790 800
AFIGTLTSDV VEDKDNSSSS GSADKVTEEE PRQGSPLTAV QLLWVNLIMD
810 820 830 840 850
TLAALALATE PPTPELLERP PNGKNAPLIT RSMWKNIIGQ AALQLAILFT
860 870 880 890 900
ILYQGHNIFQ HFVPQAHGPI IKNGLHHYTL VFNCFVFLQL FNEINARVLG
910 920 930 940 950
SRTNPFKNFF NNPIFIAVMI FTLGVQIIFV TFGGSATSTD SLYIVEWICC
960 970 980 990 1000
VVVGAISLPV GLLLRKIPIR EPVVKNEIPV HSEAVYTSPS PNPSSSNLLG
1010 1020 1030 1040 1050
SGGAKPISKD YPTSGESTPP INDEGSPLVT RKTSVGASAN DNINTPIPSS
1060 1070 1080 1090 1100
SSNLVNLNKP TQVGRGWQIV RQTHKKLVVI NALKEFSQNK EPGLVDVVRG
1110
TNRGSLHLPV NQINN
Length:1,115
Mass (Da):120,677
Last modified:December 4, 2007 - v2
Checksum:i6A80A21D490973AB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti212C → Y in CAA61551 (PubMed:7499325).Curated1
Sequence conflicti277L → H in CAA61551 (PubMed:7499325).Curated1
Sequence conflicti289 – 290GY → WL in CAA61551 (PubMed:7499325).Curated2
Sequence conflicti344G → V in CAA61551 (PubMed:7499325).Curated1
Sequence conflicti643A → V in CAA61551 (PubMed:7499325).Curated1
Sequence conflicti680T → S in CAA61551 (PubMed:7499325).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89369 mRNA Translation: CAA61551.1
AAFI02000023 Genomic DNA Translation: EAL68103.2
PIRiS57726
RefSeqiXP_642164.2, XM_637072.2

Genome annotation databases

EnsemblProtistsiEAL68103; EAL68103; DDB_G0277861
GeneIDi8621371
KEGGiddi:DDB_G0277861

Similar proteinsi

Entry informationi

Entry nameiATC1_DICDI
AccessioniPrimary (citable) accession number: P54678
Secondary accession number(s): Q54YN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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