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P54678 (ATC1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-transporting ATPase PAT1

EC=3.6.3.8
Gene names
Name:patA
ORF Names:DDB_G0277861
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1115 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium ATPase involved in Ca2+ homeostasis as a component of the contractile vacuole complex. Ref.1 Ref.3

Catalytic activity

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Subcellular location

Contractile vacuole membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note: Contractile vacuole complex. Localizes to the contractile vacule membrane in unstimulated cells. Localizes to the cell membrane and the contractile vacule membrane in cells stimulated by calcium. Ref.1 Ref.3

Developmental stage

Expressed constitutively at very low levels during vegetative growth and throughout development. Ref.1

Induction

By calcium. Ref.1 Ref.3

Miscellaneous

Loss-of-function mutant (antisense inhibition) displays impaired growth in high Ca2+ medium but normal growth in low Ca2+ medium. Antisense inhibition does not affect development in high Ca2+ medium.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11151115Calcium-transporting ATPase PAT1
PRO_0000046179

Regions

Topological domain1 – 9999Stromal Potential
Transmembrane100 – 12021Helical; Potential
Topological domain121 – 1266Lumenal Potential
Transmembrane127 – 14721Helical; Potential
Topological domain148 – 23588Stromal Potential
Transmembrane236 – 25621Helical; Potential
Topological domain257 – 28731Lumenal Potential
Transmembrane288 – 30821Helical; Potential
Topological domain309 – 32820Stromal Potential
Transmembrane329 – 34921Helical; Potential
Topological domain350 – 735386Lumenal Potential
Transmembrane736 – 75621Helical; Potential
Topological domain757 – 83276Stromal Potential
Transmembrane833 – 85321Helical; Potential
Topological domain854 – 87320Lumenal Potential
Transmembrane874 – 89421Helical; Potential
Topological domain895 – 91319Stromal Potential
Transmembrane914 – 93421Helical; Potential
Topological domain935 – 9439Lumenal Potential
Transmembrane944 – 96421Helical; Potential
Topological domain965 – 1115151Stromal Potential

Sites

Active site38514-aspartylphosphate intermediate By similarity
Metal binding6781Magnesium By similarity
Metal binding6821Magnesium By similarity

Experimental info

Sequence conflict2121C → Y in CAA61551. Ref.1
Sequence conflict2771L → H in CAA61551. Ref.1
Sequence conflict289 – 2902GY → WL in CAA61551. Ref.1
Sequence conflict3441G → V in CAA61551. Ref.1
Sequence conflict6431A → V in CAA61551. Ref.1
Sequence conflict6801T → S in CAA61551. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54678 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 6A80A21D490973AB

FASTA1,115120,677
        10         20         30         40         50         60 
MTGSHEMESI MLDSMEEEFP VSVETLGKLV DVPKGFDTYA ELGGLSGLST KLKSNIKTGL 

        70         80         90        100        110        120 
PLEKSSTEEN RVLKYSKNIL PDPPHQPLWS IVLDALSDHI LILLIVAAVV SIVLGSIDYT 

       130        140        150        160        170        180 
SDHPETGWID GVAILVAVIL VVGITSLNDF KNQARFRELN DKSNDKEVKG IRGGEQCQIS 

       190        200        210        220        230        240 
IFDVKVGDII SLDTGDIICA DGVFIEGHAL KCDESSITGE SDPIKKGQPQ DNMDPFLISG 

       250        260        270        280        290        300 
SMVIEGFGTM LVTAVGVNSF NGKTMMGLRV ASEDTPLQMK LSVLASRIGY FGMGAAILML 

       310        320        330        340        350        360 
LIAIPKYFIQ RKVHDIEITR EDAQPIVQLV ISAITIVVVA VPEGLPLAVT MALAYGMMKM 

       370        380        390        400        410        420 
FKENNLVRNL ASCETMGSAT TICSDKTGTL TQNVMSVVTG TICGVFPTLD GIAQKIPKHV 

       430        440        450        460        470        480 
QSILTDGMAI NSNAYEGVSS KGKLEFIGSK TECALLNFGK LFGCDYNEVR KRLEVVELYP 

       490        500        510        520        530        540 
FSSARKRMSV LVKHDQNLRL FTKGASEIIL GQCGSYLDEA GNIRPISEAK AYFEEQINNF 

       550        560        570        580        590        600 
ASDALRTIGL AYRDFQYGEC DFKEPPENNL VFIGIVGIKD PLRPEVPEAV EICKRAGIVV 

       610        620        630        640        650        660 
RMVTGDNLVT AQNIARNCGI LTEGGLCMEG PKFRELSQSE MDAILPKLQV LARSSPTDKQ 

       670        680        690        700        710        720 
LLVGRLKDLG EVVAVTGDGT NDGPALKLAN VGFSMGISGT EVAIAASDVV LLDDNFASIV 

       730        740        750        760        770        780 
RAVLWGRNIY DAICKFLQFQ LTVNVVAVTV AFIGTLTSDV VEDKDNSSSS GSADKVTEEE 

       790        800        810        820        830        840 
PRQGSPLTAV QLLWVNLIMD TLAALALATE PPTPELLERP PNGKNAPLIT RSMWKNIIGQ 

       850        860        870        880        890        900 
AALQLAILFT ILYQGHNIFQ HFVPQAHGPI IKNGLHHYTL VFNCFVFLQL FNEINARVLG 

       910        920        930        940        950        960 
SRTNPFKNFF NNPIFIAVMI FTLGVQIIFV TFGGSATSTD SLYIVEWICC VVVGAISLPV 

       970        980        990       1000       1010       1020 
GLLLRKIPIR EPVVKNEIPV HSEAVYTSPS PNPSSSNLLG SGGAKPISKD YPTSGESTPP 

      1030       1040       1050       1060       1070       1080 
INDEGSPLVT RKTSVGASAN DNINTPIPSS SSNLVNLNKP TQVGRGWQIV RQTHKKLVVI 

      1090       1100       1110 
NALKEFSQNK EPGLVDVVRG TNRGSLHLPV NQINN 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of an intracellular P-type ATPase from Dictyostelium that is up-regulated in calcium-adapted cells."
Moniakis J., Coukell M.B., Forer A.
J. Biol. Chem. 270:28276-28281(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"Involvement of the Ca2+-ATPase PAT1 and the contractile vacuole in calcium regulation in Dictyostelium discoideum."
Moniakis J., Coukell M.B., Janiec A.
J. Cell Sci. 112:405-414(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89369 mRNA. Translation: CAA61551.1.
AAFI02000023 Genomic DNA. Translation: EAL68103.2.
PIRS57726.
RefSeqXP_642164.2. XM_637072.2.

3D structure databases

ProteinModelPortalP54678.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0214945.

Protein family/group databases

TCDB3.A.3.2.17. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PRIDEP54678.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0214945; DDB0214945; DDB_G0277861.
GeneID8621371.
KEGGddi:DDB_G0277861.

Organism-specific databases

dictyBaseDDB_G0277861. patA.

Phylogenomic databases

eggNOGCOG0474.
OMASHEMESI.
PhylomeDBP54678.

Family and domain databases

Gene3D1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameATC1_DICDI
AccessionPrimary (citable) accession number: P54678
Secondary accession number(s): Q54YN8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: July 9, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase