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P54678

- ATC1_DICDI

UniProt

P54678 - ATC1_DICDI

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Protein
Calcium-transporting ATPase PAT1
Gene
patA, DDB_G0277861
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium ATPase involved in Ca2+ homeostasis as a component of the contractile vacuole complex.2 Publications

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei385 – 38514-aspartylphosphate intermediate By similarity
Metal bindingi678 – 6781Magnesium By similarity
Metal bindingi682 – 6821Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: dictyBase
  2. calcium ion binding Source: dictyBase
  3. calcium ion transmembrane transporter activity Source: dictyBase
  4. calcium-transporting ATPase activity Source: UniProtKB

GO - Biological processi

  1. calcium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.3.2.17. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase PAT1 (EC:3.6.3.8)
Gene namesi
Name:patA
ORF Names:DDB_G0277861
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0277861. patA.

Subcellular locationi

Contractile vacuole membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Note: Contractile vacuole complex. Localizes to the contractile vacule membrane in unstimulated cells. Localizes to the cell membrane and the contractile vacule membrane in cells stimulated by calcium.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9999Stromal Reviewed prediction
Add
BLAST
Transmembranei100 – 12021Helical; Reviewed prediction
Add
BLAST
Topological domaini121 – 1266Lumenal Reviewed prediction
Transmembranei127 – 14721Helical; Reviewed prediction
Add
BLAST
Topological domaini148 – 23588Stromal Reviewed prediction
Add
BLAST
Transmembranei236 – 25621Helical; Reviewed prediction
Add
BLAST
Topological domaini257 – 28731Lumenal Reviewed prediction
Add
BLAST
Transmembranei288 – 30821Helical; Reviewed prediction
Add
BLAST
Topological domaini309 – 32820Stromal Reviewed prediction
Add
BLAST
Transmembranei329 – 34921Helical; Reviewed prediction
Add
BLAST
Topological domaini350 – 735386Lumenal Reviewed prediction
Add
BLAST
Transmembranei736 – 75621Helical; Reviewed prediction
Add
BLAST
Topological domaini757 – 83276Stromal Reviewed prediction
Add
BLAST
Transmembranei833 – 85321Helical; Reviewed prediction
Add
BLAST
Topological domaini854 – 87320Lumenal Reviewed prediction
Add
BLAST
Transmembranei874 – 89421Helical; Reviewed prediction
Add
BLAST
Topological domaini895 – 91319Stromal Reviewed prediction
Add
BLAST
Transmembranei914 – 93421Helical; Reviewed prediction
Add
BLAST
Topological domaini935 – 9439Lumenal Reviewed prediction
Transmembranei944 – 96421Helical; Reviewed prediction
Add
BLAST
Topological domaini965 – 1115151Stromal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. contractile vacuolar membrane Source: UniProtKB
  2. integral component of membrane Source: dictyBase
  3. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11151115Calcium-transporting ATPase PAT1
PRO_0000046179Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP54678.

Expressioni

Developmental stagei

Expressed constitutively at very low levels during vegetative growth and throughout development.1 Publication

Inductioni

By calcium.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0214945.

Structurei

3D structure databases

ProteinModelPortaliP54678.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
KOiK01537.
OMAiSHEMESI.
PhylomeDBiP54678.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54678-1 [UniParc]FASTAAdd to Basket

« Hide

MTGSHEMESI MLDSMEEEFP VSVETLGKLV DVPKGFDTYA ELGGLSGLST     50
KLKSNIKTGL PLEKSSTEEN RVLKYSKNIL PDPPHQPLWS IVLDALSDHI 100
LILLIVAAVV SIVLGSIDYT SDHPETGWID GVAILVAVIL VVGITSLNDF 150
KNQARFRELN DKSNDKEVKG IRGGEQCQIS IFDVKVGDII SLDTGDIICA 200
DGVFIEGHAL KCDESSITGE SDPIKKGQPQ DNMDPFLISG SMVIEGFGTM 250
LVTAVGVNSF NGKTMMGLRV ASEDTPLQMK LSVLASRIGY FGMGAAILML 300
LIAIPKYFIQ RKVHDIEITR EDAQPIVQLV ISAITIVVVA VPEGLPLAVT 350
MALAYGMMKM FKENNLVRNL ASCETMGSAT TICSDKTGTL TQNVMSVVTG 400
TICGVFPTLD GIAQKIPKHV QSILTDGMAI NSNAYEGVSS KGKLEFIGSK 450
TECALLNFGK LFGCDYNEVR KRLEVVELYP FSSARKRMSV LVKHDQNLRL 500
FTKGASEIIL GQCGSYLDEA GNIRPISEAK AYFEEQINNF ASDALRTIGL 550
AYRDFQYGEC DFKEPPENNL VFIGIVGIKD PLRPEVPEAV EICKRAGIVV 600
RMVTGDNLVT AQNIARNCGI LTEGGLCMEG PKFRELSQSE MDAILPKLQV 650
LARSSPTDKQ LLVGRLKDLG EVVAVTGDGT NDGPALKLAN VGFSMGISGT 700
EVAIAASDVV LLDDNFASIV RAVLWGRNIY DAICKFLQFQ LTVNVVAVTV 750
AFIGTLTSDV VEDKDNSSSS GSADKVTEEE PRQGSPLTAV QLLWVNLIMD 800
TLAALALATE PPTPELLERP PNGKNAPLIT RSMWKNIIGQ AALQLAILFT 850
ILYQGHNIFQ HFVPQAHGPI IKNGLHHYTL VFNCFVFLQL FNEINARVLG 900
SRTNPFKNFF NNPIFIAVMI FTLGVQIIFV TFGGSATSTD SLYIVEWICC 950
VVVGAISLPV GLLLRKIPIR EPVVKNEIPV HSEAVYTSPS PNPSSSNLLG 1000
SGGAKPISKD YPTSGESTPP INDEGSPLVT RKTSVGASAN DNINTPIPSS 1050
SSNLVNLNKP TQVGRGWQIV RQTHKKLVVI NALKEFSQNK EPGLVDVVRG 1100
TNRGSLHLPV NQINN 1115
Length:1,115
Mass (Da):120,677
Last modified:December 4, 2007 - v2
Checksum:i6A80A21D490973AB
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti212 – 2121C → Y in CAA61551. 1 Publication
Sequence conflicti277 – 2771L → H in CAA61551. 1 Publication
Sequence conflicti289 – 2902GY → WL in CAA61551. 1 Publication
Sequence conflicti344 – 3441G → V in CAA61551. 1 Publication
Sequence conflicti643 – 6431A → V in CAA61551. 1 Publication
Sequence conflicti680 – 6801T → S in CAA61551. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89369 mRNA. Translation: CAA61551.1.
AAFI02000023 Genomic DNA. Translation: EAL68103.2.
PIRiS57726.
RefSeqiXP_642164.2. XM_637072.2.

Genome annotation databases

EnsemblProtistsiDDB0214945; DDB0214945; DDB_G0277861.
GeneIDi8621371.
KEGGiddi:DDB_G0277861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89369 mRNA. Translation: CAA61551.1 .
AAFI02000023 Genomic DNA. Translation: EAL68103.2 .
PIRi S57726.
RefSeqi XP_642164.2. XM_637072.2.

3D structure databases

ProteinModelPortali P54678.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0214945.

Protein family/group databases

TCDBi 3.A.3.2.17. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PRIDEi P54678.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0214945 ; DDB0214945 ; DDB_G0277861 .
GeneIDi 8621371.
KEGGi ddi:DDB_G0277861.

Organism-specific databases

dictyBasei DDB_G0277861. patA.

Phylogenomic databases

eggNOGi COG0474.
KOi K01537.
OMAi SHEMESI.
PhylomeDBi P54678.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
PR00120. HATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of an intracellular P-type ATPase from Dictyostelium that is up-regulated in calcium-adapted cells."
    Moniakis J., Coukell M.B., Forer A.
    J. Biol. Chem. 270:28276-28281(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "Involvement of the Ca2+-ATPase PAT1 and the contractile vacuole in calcium regulation in Dictyostelium discoideum."
    Moniakis J., Coukell M.B., Janiec A.
    J. Cell Sci. 112:405-414(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.

Entry informationi

Entry nameiATC1_DICDI
AccessioniPrimary (citable) accession number: P54678
Secondary accession number(s): Q54YN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Loss-of-function mutant (antisense inhibition) displays impaired growth in high Ca2+ medium but normal growth in low Ca2+ medium. Antisense inhibition does not affect development in high Ca2+ medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi