Reviewed, UniProtKB/Swiss-Prot P54677 (PI4K_DICDI)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase
      Short name=PtdIns-4-kinase
      Short name=PI4-kinase
    EC=2.7.1.67
Alternative name(s):
    PI4K-alpha

Gene names

Name:	pikD
Synonyms:	pik4
ORF Names:	DDB_G0288485

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoinositide phosphorylation Inferred from electronic annotation. Source: InterPro phosphoinositide-mediated signaling Inferred from electronic annotation. Source: InterPro
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1180	1180	Phosphatidylinositol 4-kinase		PRO_0000088826
Regions
	Domain	919 – 1178	260	PI3K/PI4K
	Compositional bias	21 – 27	7	Poly-Asn
	Compositional bias	38 – 43	6	Poly-Asn
	Compositional bias	62 – 69	8	Poly-Ser
	Compositional bias	70 – 74	5	Poly-Asn
	Compositional bias	233 – 238	6	Poly-Asp
	Compositional bias	271 – 287	17	Poly-Asn
	Compositional bias	290 – 293	4	Poly-Asn
	Compositional bias	314 – 320	7	Poly-Asn
	Compositional bias	364 – 402	39	Poly-Asn
	Compositional bias	528 – 532	5	Poly-Asp
	Compositional bias	542 – 553	12	Poly-Thr
	Compositional bias	581 – 588	8	Poly-Gly
	Compositional bias	777 – 783	7	Poly-Thr
	Compositional bias	838 – 841	4	Poly-Gln
	Compositional bias	848 – 859	12	Poly-Thr
	Compositional bias	862 – 872	11	Poly-Thr
Experimental info
	Sequence conflict	138	1	T → N in AAA85725. Ref.2
	Sequence conflict	149	1	H → L in AAA85725. Ref.2
	Sequence conflict	252 – 253	2	ND → KC in AAA85725. Ref.2
	Sequence conflict	326	1	D → N in AAA85725. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P54677-1 [UniParc]. Last modified September 2, 2008. Version 3. Checksum: 1AB9290C91445F32	FASTA	1,180	132,681
	10 20 30 40 50 60 MNKISDTIII TSTSNEDEVD NNNNNNNLKE IDRSPRVNNN NNNILTNVNN NKNNTITSSG 70 80 90 100 110 120 GSDSSSSSSN NNNNKIKKSK KHKEKEHMDS IVKLYSGKFD SWMVICHLFK YRDNPGIVDF 130 140 150 160 170 180 LCNKMYNLED KDIDFYITQL CILLINQPHD QKASFSSLAR FILDRCASSF RFAIKAYWIF 190 200 210 220 230 240 QAFEEDGEKN LFSIEGSVYL HSPSTSPKDV PMYSNDQIVP IDLDKIYNQS QYDDDDFDLS 250 260 270 280 290 300 DDDGGFEIIK KNDHHYENDH HIENDPKKDI NSNNNNNNNI NNNNSNNDDN NNNEILPNEN 310 320 330 340 350 360 SDNSINDENN QYGNSNNNNN ISGENDNIKI DINSQNKSDS NIETLNSTLC EETKTSPIKD 370 380 390 400 410 420 DMENNNNNNN NNNNNNNNNN NNNNINNNNI NNNNINNNNN INYGHINGSL STLDGIGQPY 430 440 450 460 470 480 ISQPNDPIEN ITQILKRNRI IYKKVEEKKE LATRLREFCE MSVITCSRPL ITRPRTSSLP 490 500 510 520 530 540 SPLISYNSGK IGGNYHKILS PSSVDSTSLI SEDDKIIEKE EEDNVVEDDD DDEVNSEDFI 550 560 570 580 590 600 PTATTTATTT TTTIPNHLSK TTSGVGINSN SSTPININSA GAGAGGGGEI NHIGYDDISY 610 620 630 640 650 660 LDKCKTPPAE SKLSDHDFEF ELSKSHRCDY LNDILSFIQK LAHISKILLP IPIDLRQAKL 670 680 690 700 710 720 KHEISLLNIN LPLGLYVPLW QSSNHHCVVR IPPEEVKILN SRERVPFLLV LEVIESEHEA 730 740 750 760 770 780 LSSNIFEVVS SYLQYTTGNS ALKKDDIKRK YYSEKFKKSF LNSSINSTIS NSSDSCTTET 790 800 810 820 830 840 TTTSPVATSP TLPINIPHSK LINDGSNSIS KSLPVTPTQS TVLNNLISTS TAISPPSQQQ 850 860 870 880 890 900 QLPSPSNTTT TTTTTTTNTN NTTTTTTTTT TTLSTSPTNE KILNENKQNS SPFGESWQEK 910 920 930 940 950 960 IERYKKISPF GDYPNWRLYS VIVKTGDDCR QEQMAVQLIS KFDEIWKETR LPLYLRPYSI 970 980 990 1000 1010 1020 LVTSSGGGII ETIPDTMSLH NLKKSTPGFT TLLNYFKSTY GDPSGLRFRT AQSNFIESMA 1030 1040 1050 1060 1070 1080 AYSIVTYILQ IKDRHNGNIL IDKEGHIVHI DFGFILSNSP GNISFESAPF KLTQELVDVM 1090 1100 1110 1120 1130 1140 GGIQSGQFQY FKVLCVRGLI EARKQVDKII SLIEIMMSGP KMSCFVGGKE VIEQLKARFF 1150 1160 1170 1180 LDVNERECST LVENLISYSI DHFKTRYYDK YQSWLNGIYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development." Zhou K., Takegawa K., Emr S.D., Firtel R.A. Mol. Cell. Biol. 15:5645-5656(1995) [PubMed: 7565716] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 88-1180. Strain: AX3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000112 Genomic DNA. Translation: EAL63191.2. U23479 Genomic DNA. Translation: AAA85725.1.
PIR	T18275.
RefSeq	XP_636702.2.
3D structure databases
HSSP	HSSP built from PDB template 1HE8 based on UniProtKB P48736.
ModBase	Search...
Protein-protein interaction databases
STRING	P54677.
Genome annotation databases
GeneID	3388268.
GenomeReviews	Gene locus pikD in contig CM000154_GR.
KEGG	ddi:DDB_0191346.
Organism-specific databases
dictyBase	DDB_G0288485. pikD.
Enzyme and pathway databases
BRENDA	2.7.1.67. 424.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI4K_DICDI

Accession

Primary (citable) accession number: P54677
Secondary accession number(s): Q54IV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 2, 2008
Last modified:	September 22, 2009
This is version 50 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)