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P54676

- PI3K4_DICDI

UniProt

P54676 - PI3K4_DICDI

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Protein

Phosphatidylinositol 3-kinase VPS34-like

Gene

pikE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

  1. 1-phosphatidylinositol-3-kinase activity Source: RefGenome
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
  2. phosphatidylinositol-mediated signaling Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.
ReactomeiREACT_207952. Synthesis of PIPs at the early endosome membrane.
REACT_209866. Synthesis of PIPs at the Golgi membrane.
REACT_210188. Synthesis of PIPs at the late endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase VPS34-like (EC:2.7.1.137)
Short name:
PI3-kinase
Short name:
PI3K
Short name:
PtdIns-3-kinase
Gene namesi
Name:pikE
Synonyms:pik5
ORF Names:DDB_G0289601
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0289601. pikE.

Subcellular locationi

GO - Cellular componenti

  1. phosphatidylinositol 3-kinase complex Source: RefGenome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 816816Phosphatidylinositol 3-kinase VPS34-likePRO_0000088825Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0219939.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 186148C2 PI3K-typePROSITE-ProRule annotationAdd
BLAST
Domaini275 – 460186PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini559 – 816258PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5032.
InParanoidiP54676.
KOiK00914.
OMAiYLMVEFP.
PhylomeDBiP54676.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
SMARTiSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54676-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVSKDFKFY YSRDIPIKYK IKLCTLEGKR NKENAPETVD KWIEKKYLKY
60 70 80 90 100
SSLYRDTTID LYITCTLYSD GKQLSTSEHT SYNPFSVSNK WDETIIFPLK
110 120 130 140 150
HQDLPFDTMI VITIWDIYSP MKKVPIGGTS FNIFGMNKIE RKGKHKLLIW
160 170 180 190 200
QDREGDGEKE TTTPGQIQGK DEQYRLEKLK KKYDRKLINH CQWLDKFSLN
210 220 230 240 250
EIERLSRASE SLNKNQIYLT IELPEFELPI LFKQQNCPLY IPLSQTQKKS
260 270 280 290 300
LVLINDIEMD EHPSEQKYHR LNLYDHKDLK PNSTELKGLT DILKQPPNTK
310 320 330 340 350
VTSKEALLIW RFRYYLTNNK KALTKFLRCV EWSESHQKNE ALSIMPKWDP
360 370 380 390 400
IDIADSLELL SSAFTHKNTI MVRRYAVEIL KKADDEELLY YLLQLVQATK
410 420 430 440 450
YEQFDGNPSD SPLISFLFER SNKNFILGSH FYWYLTVDSV LKSSIFCSHY
460 470 480 490 500
KTLQELYFRQ LDHTDAQRVN AQNKFISRLS LLSVELKAMN ITREKKIEKL
510 520 530 540 550
RIMLAEGEYK DLSDFQPIRL PVNPDIEIIG IVPEKSNIYK SAKSPLGLKL
560 570 580 590 600
RTTKGEEYGV IFKTGDDLRQ DQLIIQLISL MDRLLKKENL DLKLTPYKVL
610 620 630 640 650
ATAEEDGIVE MVNPSEAMAS VLSKYDGDIL KFFKTHNPDA DSPYGIAPEV
660 670 680 690 700
MDTFVKSCAG YCVITYILGI GDRHLDNLLL TPNGKLFHID FGYILGKDPK
710 720 730 740 750
ILPPPMKLCK EMVLGMGGEN SKHYEKFKQL CCEAYNILRK SSHLILNLFA
760 770 780 790 800
LMVDASIPSI SDDKEKSILK VQEKLQLELT DQEASNSLLQ LLNDSVTALF
810
PVIIEMAHKW LQYWKA
Length:816
Mass (Da):94,748
Last modified:December 4, 2007 - v2
Checksum:iDC6637F485FD6975
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41S → T in AAA85726. (PubMed:7565716)Curated
Sequence conflicti13 – 131R → S in AAA85726. (PubMed:7565716)Curated
Sequence conflicti44 – 463EKK → DDP in AAA85726. (PubMed:7565716)Curated
Sequence conflicti289 – 2902LT → NS in AAA85726. (PubMed:7565716)Curated
Sequence conflicti382 – 3821K → P in AAA85726. (PubMed:7565716)Curated
Sequence conflicti565 – 5651G → N in AAA85726. (PubMed:7565716)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23480 Genomic DNA. Translation: AAA85726.1.
AAFI02000147 Genomic DNA. Translation: EAL62618.1.
PIRiA59003.
RefSeqiXP_636122.1. XM_631030.1.

Genome annotation databases

EnsemblProtistsiDDB0219939; DDB0219939; DDB_G0289601.
GeneIDi8627225.
KEGGiddi:DDB_G0289601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23480 Genomic DNA. Translation: AAA85726.1 .
AAFI02000147 Genomic DNA. Translation: EAL62618.1 .
PIRi A59003.
RefSeqi XP_636122.1. XM_631030.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0219939.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0219939 ; DDB0219939 ; DDB_G0289601 .
GeneIDi 8627225.
KEGGi ddi:DDB_G0289601.

Organism-specific databases

dictyBasei DDB_G0289601. pikE.

Phylogenomic databases

eggNOGi COG5032.
InParanoidi P54676.
KOi K00914.
OMAi YLMVEFP.
PhylomeDBi P54676.

Enzyme and pathway databases

BRENDAi 2.7.1.137. 1939.
Reactomei REACT_207952. Synthesis of PIPs at the early endosome membrane.
REACT_209866. Synthesis of PIPs at the Golgi membrane.
REACT_210188. Synthesis of PIPs at the late endosome membrane.

Miscellaneous databases

PROi P54676.

Family and domain databases

Gene3Di 1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProi IPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view ]
PANTHERi PTHR10048. PTHR10048. 1 hit.
Pfami PF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view ]
PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
SMARTi SM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development."
    Zhou K., Takegawa K., Emr S.D., Firtel R.A.
    Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPI3K4_DICDI
AccessioniPrimary (citable) accession number: P54676
Secondary accession number(s): Q54HA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3