P54676 (PI3K4_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase VPS34-like
Short name=PI3-kinase
Short name=PI3K
Short name=PtdIns-3-kinase
EC=2.7.1.137

Gene names

Name:	pikE
Synonyms:	pik5
ORF Names:	DDB_G0289601

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular_component	phosphatidylinositol 3-kinase complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	1-phosphatidylinositol-3-kinase activity Inferred from Biological aspect of Ancestor. Source: RefGenome ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 816	816	Phosphatidylinositol 3-kinase VPS34-like		PRO_0000088825
Regions
	Domain	39 – 186	148	C2 PI3K-type
	Domain	275 – 460	186	PIK helical
	Domain	559 – 816	258	PI3K/PI4K
Experimental info
	Sequence conflict	4	1	S → T in AAA85726. Ref.1
	Sequence conflict	13	1	R → S in AAA85726. Ref.1
	Sequence conflict	44 – 46	3	EKK → DDP in AAA85726. Ref.1
	Sequence conflict	289 – 290	2	LT → NS in AAA85726. Ref.1
	Sequence conflict	382	1	K → P in AAA85726. Ref.1
	Sequence conflict	565	1	G → N in AAA85726. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54676 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: DC6637F485FD6975	FASTA	816	94,748
	10 20 30 40 50 60 MKVSKDFKFY YSRDIPIKYK IKLCTLEGKR NKENAPETVD KWIEKKYLKY SSLYRDTTID 70 80 90 100 110 120 LYITCTLYSD GKQLSTSEHT SYNPFSVSNK WDETIIFPLK HQDLPFDTMI VITIWDIYSP 130 140 150 160 170 180 MKKVPIGGTS FNIFGMNKIE RKGKHKLLIW QDREGDGEKE TTTPGQIQGK DEQYRLEKLK 190 200 210 220 230 240 KKYDRKLINH CQWLDKFSLN EIERLSRASE SLNKNQIYLT IELPEFELPI LFKQQNCPLY 250 260 270 280 290 300 IPLSQTQKKS LVLINDIEMD EHPSEQKYHR LNLYDHKDLK PNSTELKGLT DILKQPPNTK 310 320 330 340 350 360 VTSKEALLIW RFRYYLTNNK KALTKFLRCV EWSESHQKNE ALSIMPKWDP IDIADSLELL 370 380 390 400 410 420 SSAFTHKNTI MVRRYAVEIL KKADDEELLY YLLQLVQATK YEQFDGNPSD SPLISFLFER 430 440 450 460 470 480 SNKNFILGSH FYWYLTVDSV LKSSIFCSHY KTLQELYFRQ LDHTDAQRVN AQNKFISRLS 490 500 510 520 530 540 LLSVELKAMN ITREKKIEKL RIMLAEGEYK DLSDFQPIRL PVNPDIEIIG IVPEKSNIYK 550 560 570 580 590 600 SAKSPLGLKL RTTKGEEYGV IFKTGDDLRQ DQLIIQLISL MDRLLKKENL DLKLTPYKVL 610 620 630 640 650 660 ATAEEDGIVE MVNPSEAMAS VLSKYDGDIL KFFKTHNPDA DSPYGIAPEV MDTFVKSCAG 670 680 690 700 710 720 YCVITYILGI GDRHLDNLLL TPNGKLFHID FGYILGKDPK ILPPPMKLCK EMVLGMGGEN 730 740 750 760 770 780 SKHYEKFKQL CCEAYNILRK SSHLILNLFA LMVDASIPSI SDDKEKSILK VQEKLQLELT 790 800 810 DQEASNSLLQ LLNDSVTALF PVIIEMAHKW LQYWKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development." Zhou K., Takegawa K., Emr S.D., Firtel R.A. Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U23480 Genomic DNA. Translation: AAA85726.1. AAFI02000147 Genomic DNA. Translation: EAL62618.1.
PIR	A59003.
RefSeq	XP_636122.1. XM_631030.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0219939.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0219939; DDB0219939; DDB_G0289601.
GeneID	8627225.
KEGG	ddi:DDB_G0289601.
Organism-specific databases
dictyBase	DDB_G0289601. pikE.
Phylogenomic databases
eggNOG	COG5032.
KO	K00914.
OMA	YLMVEFP.
ProtClustDB	CLSZ2846637.
Enzyme and pathway databases
BRENDA	2.7.1.137. 1939.
Family and domain databases
Gene3D	1.10.1070.11. 1 hit. 1.25.40.70. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2_dom. IPR008290. PI3K_Vps34. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
PANTHER	PTHR10048. PTHR10048. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
PIRSF	PIRSF000587. PI3K_Vps34. 1 hit.
SMART	SM00239. C2. 1 hit. SM00142. PI3K_C2. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51547. PI3K_C2. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI3K4_DICDI

Accession

Primary (citable) accession number: P54676
Secondary accession number(s): Q54HA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)