Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P54676

- PI3K4_DICDI

UniProt

P54676 - PI3K4_DICDI

Protein

Phosphatidylinositol 3-kinase VPS34-like

Gene

pikE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-3-kinase activity Source: RefGenome
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
    2. phosphatidylinositol-mediated signaling Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.137. 1939.
    ReactomeiREACT_207952. Synthesis of PIPs at the early endosome membrane.
    REACT_209866. Synthesis of PIPs at the Golgi membrane.
    REACT_210188. Synthesis of PIPs at the late endosome membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 3-kinase VPS34-like (EC:2.7.1.137)
    Short name:
    PI3-kinase
    Short name:
    PI3K
    Short name:
    PtdIns-3-kinase
    Gene namesi
    Name:pikE
    Synonyms:pik5
    ORF Names:DDB_G0289601
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0289601. pikE.

    Subcellular locationi

    GO - Cellular componenti

    1. phosphatidylinositol 3-kinase complex Source: RefGenome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 816816Phosphatidylinositol 3-kinase VPS34-likePRO_0000088825Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0219939.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 186148C2 PI3K-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini275 – 460186PIK helicalPROSITE-ProRule annotationAdd
    BLAST
    Domaini559 – 816258PI3K/PI4KPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
    Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
    Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
    Contains 1 PIK helical domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5032.
    KOiK00914.
    OMAiYLMVEFP.
    PhylomeDBiP54676.

    Family and domain databases

    Gene3Di1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view]
    PANTHERiPTHR10048. PTHR10048. 1 hit.
    PfamiPF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
    SMARTiSM00239. C2. 1 hit.
    SM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54676-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVSKDFKFY YSRDIPIKYK IKLCTLEGKR NKENAPETVD KWIEKKYLKY    50
    SSLYRDTTID LYITCTLYSD GKQLSTSEHT SYNPFSVSNK WDETIIFPLK 100
    HQDLPFDTMI VITIWDIYSP MKKVPIGGTS FNIFGMNKIE RKGKHKLLIW 150
    QDREGDGEKE TTTPGQIQGK DEQYRLEKLK KKYDRKLINH CQWLDKFSLN 200
    EIERLSRASE SLNKNQIYLT IELPEFELPI LFKQQNCPLY IPLSQTQKKS 250
    LVLINDIEMD EHPSEQKYHR LNLYDHKDLK PNSTELKGLT DILKQPPNTK 300
    VTSKEALLIW RFRYYLTNNK KALTKFLRCV EWSESHQKNE ALSIMPKWDP 350
    IDIADSLELL SSAFTHKNTI MVRRYAVEIL KKADDEELLY YLLQLVQATK 400
    YEQFDGNPSD SPLISFLFER SNKNFILGSH FYWYLTVDSV LKSSIFCSHY 450
    KTLQELYFRQ LDHTDAQRVN AQNKFISRLS LLSVELKAMN ITREKKIEKL 500
    RIMLAEGEYK DLSDFQPIRL PVNPDIEIIG IVPEKSNIYK SAKSPLGLKL 550
    RTTKGEEYGV IFKTGDDLRQ DQLIIQLISL MDRLLKKENL DLKLTPYKVL 600
    ATAEEDGIVE MVNPSEAMAS VLSKYDGDIL KFFKTHNPDA DSPYGIAPEV 650
    MDTFVKSCAG YCVITYILGI GDRHLDNLLL TPNGKLFHID FGYILGKDPK 700
    ILPPPMKLCK EMVLGMGGEN SKHYEKFKQL CCEAYNILRK SSHLILNLFA 750
    LMVDASIPSI SDDKEKSILK VQEKLQLELT DQEASNSLLQ LLNDSVTALF 800
    PVIIEMAHKW LQYWKA 816
    Length:816
    Mass (Da):94,748
    Last modified:December 4, 2007 - v2
    Checksum:iDC6637F485FD6975
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41S → T in AAA85726. (PubMed:7565716)Curated
    Sequence conflicti13 – 131R → S in AAA85726. (PubMed:7565716)Curated
    Sequence conflicti44 – 463EKK → DDP in AAA85726. (PubMed:7565716)Curated
    Sequence conflicti289 – 2902LT → NS in AAA85726. (PubMed:7565716)Curated
    Sequence conflicti382 – 3821K → P in AAA85726. (PubMed:7565716)Curated
    Sequence conflicti565 – 5651G → N in AAA85726. (PubMed:7565716)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23480 Genomic DNA. Translation: AAA85726.1.
    AAFI02000147 Genomic DNA. Translation: EAL62618.1.
    PIRiA59003.
    RefSeqiXP_636122.1. XM_631030.1.

    Genome annotation databases

    EnsemblProtistsiDDB0219939; DDB0219939; DDB_G0289601.
    GeneIDi8627225.
    KEGGiddi:DDB_G0289601.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23480 Genomic DNA. Translation: AAA85726.1 .
    AAFI02000147 Genomic DNA. Translation: EAL62618.1 .
    PIRi A59003.
    RefSeqi XP_636122.1. XM_631030.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0219939.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0219939 ; DDB0219939 ; DDB_G0289601 .
    GeneIDi 8627225.
    KEGGi ddi:DDB_G0289601.

    Organism-specific databases

    dictyBasei DDB_G0289601. pikE.

    Phylogenomic databases

    eggNOGi COG5032.
    KOi K00914.
    OMAi YLMVEFP.
    PhylomeDBi P54676.

    Enzyme and pathway databases

    BRENDAi 2.7.1.137. 1939.
    Reactomei REACT_207952. Synthesis of PIPs at the early endosome membrane.
    REACT_209866. Synthesis of PIPs at the Golgi membrane.
    REACT_210188. Synthesis of PIPs at the late endosome membrane.

    Miscellaneous databases

    PROi P54676.

    Family and domain databases

    Gene3Di 1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view ]
    PANTHERi PTHR10048. PTHR10048. 1 hit.
    Pfami PF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
    SMARTi SM00239. C2. 1 hit.
    SM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development."
      Zhou K., Takegawa K., Emr S.D., Firtel R.A.
      Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AX3.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPI3K4_DICDI
    AccessioniPrimary (citable) accession number: P54676
    Secondary accession number(s): Q54HA2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3