P54675 (PI3K3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase 3
Short name=PI3-kinase
Short name=PI3K
Short name=PtdIns-3-kinase
EC=2.7.1.137

Gene names

Name:	pikC
Synonyms:	pik3
ORF Names:	DDB_G0275011

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1697 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K-RBD domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular component movement Inferred from genetic interaction PubMed 17462897. Source: dictyBase phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro response to bacterium Inferred from mutant phenotype PubMed 21045112. Source: dictyBase
Cellular_component	phosphatidylinositol 3-kinase complex Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	1-phosphatidylinositol-3-kinase activity Traceable author statement PubMed 10878573. Source: dictyBase 1-phosphatidylinositol-4-phosphate 3-kinase activity Inferred from Biological aspect of Ancestor. Source: RefGenome ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1697	1697	Phosphatidylinositol 3-kinase 3		PRO_0000088824
Regions
	Domain	737 – 823	87	PI3K-RBD
	Domain	888 – 1036	149	C2 PI3K-type
	Domain	1060 – 1238	179	PIK helical
	Domain	1333 – 1596	264	PI3K/PI4K
	Repeat	1622 – 1626	5	1
	Repeat	1627 – 1631	5	2
	Repeat	1632 – 1636	5	3
	Repeat	1642 – 1646	5	4
	Repeat	1647 – 1651	5	5
	Region	1622 – 1651	30	5 X 5 AA approximate repeats
	Region	1659 – 1672	14	7 X 2 AA tandem repeats of K-E
	Compositional bias	170 – 196	27	Poly-Asn
	Compositional bias	210 – 222	13	Poly-Asn
	Compositional bias	312 – 338	27	Poly-Asn
	Compositional bias	351 – 366	16	Poly-Asn
	Compositional bias	457 – 490	34	Poly-Asn
	Compositional bias	495 – 502	8	Poly-Asn
	Compositional bias	596 – 600	5	Poly-Ser
	Compositional bias	832 – 849	18	Poly-Gln
	Compositional bias	1681 – 1687	7	Poly-Asn
Experimental info
	Sequence conflict	1159	1	A → T in AAA85723. Ref.3
	Sequence conflict	1175	1	M → L in AAA85723. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P54675 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: C5442DC2C3202E32	FASTA	1,697	192,942
	10 20 30 40 50 60 MRQIVTGVIH QTTQSQQIPN VINSNQIQFS NEPMVVGSIE DFDIDSEVPP LAINLQRSIN 70 80 90 100 110 120 NNNNNNNNNN NNNNNNNNNN NNNNNNNTQP CTTVFLDRDS CVNVKATIDL LKEQLEFTIK 130 140 150 160 170 180 DLIDFKENYD KLESTEQFKQ WSNLIKNIKE NSLNNSNIYL TIPTTQNLIN NNNNNNNNNN 190 200 210 220 230 240 NNNNNNNNNN NNNNNNVIIP SASTENKEEN DNNNSNNNNN INLSPDSSIT KDINITENKI 250 260 270 280 290 300 TEIKTTETKE TSTGTSPLEK SPSKGFIISP KKPEEENEIE GETINNIAIT NYTQGPSMLT 310 320 330 340 350 360 LMKKKLENIK KNNNNNNNNG NGNNNSNNNN SNSNNNNNGI SPSSSPPSHL NGNNNNNNSN 370 380 390 400 410 420 NTNSNNTTNA TTNSVGFSIT MTNSNSLSVS KRMNKFKSWT SSKPTSSSIG FASSPQNNGK 430 440 450 460 470 480 PLNISGSSRF FTSRQDSKID LLKSPSSSPP TQSDIFNENN NNNNNNNNNN NNNNNNNNNN 490 500 510 520 530 540 NNNNNNNNNN EELINNNNNN NNDENYKIEE TEESLKELLE KEKLENEERE KILKERNEID 550 560 570 580 590 600 NLKKKNHLSK GYFMRACNAS NDDGLEEEDI PLQDEHWETN VIVLLPCRHH VKVPGSSSSS 610 620 630 640 650 660 IDSIRQLAWA SGKMQGHLNL EKDEKFFTLR WCNKDVVFDQ DTPLGHLIQY NLNYNNPTQK 670 680 690 700 710 720 PTNIKLELVL EDELCKERLV DLQSLEINNG RPSIWKSHID DVLSFNRKLR ELAMLAKPQS 730 740 750 760 770 780 NVPAARLTPY PPPKTIPEFF VIRVHLFKNQ TKSLRCANNH TAFSLMTILS EKLKNTTPFD 790 800 810 820 830 840 PTQYRFLITG INQYVDPNVP LLSVEYIVEK IKRKGEIDLT MVELLSLGLI IQQQQQQQQQ 850 860 870 880 890 900 QQQQQQQQQI ENIDDENILK LNNGILNVLS KIEKPIREKD NCISSLTVTE NLQVRLLHAH 910 920 930 940 950 960 EIFASKASEI IGTDSPSIQL FIEAAVYFGG ELLATQSSKL VSFQDTVVWN EWVNIPLAVS 970 980 990 1000 1010 1020 NIPNGARMCL GLNARYRGDI FNIGWVGHRL FDSKGILNTF APFSLLLWPG KINPIGTCVD 1030 1040 1050 1060 1070 1080 NLESKDQAII IAFEFKDYVV PKTIHYEDDL IELISKDENG NELPVVTMEE MDRVEQIILQ 1090 1100 1110 1120 1130 1140 DPLYSLNKEE RLLIWKSRYF CHTKPQALSK LLQSVEWTNY KQVGEAFQLL KIWPTLSAVD 1150 1160 1170 1180 1190 1200 ALELLDPKFA DCVEIREYAV KCLDQMSDYE LEIYMLQLVQ AIKHDVFHNS VLSLFLIGRV 1210 1220 1230 1240 1250 1260 WQNMQVLGHP FFWHLRADID NQEVCERFRV LSSGFLRYAP TQLMESFKRE ITTLRILENL 1270 1280 1290 1300 1310 1320 AKRVKEVPYE KRKQYVENNL REEQSFPTEL FVPFDPSIRI LNIIPEKCKS MDSAKVPLWV 1330 1340 1350 1360 1370 1380 TFKNADPFAP PLQMIAKTGD DLRQDILTLQ LLRLMDHMWK SQDLDLHMTI YRCIATGMGT 1390 1400 1410 1420 1430 1440 GLIEVVPNSE TAARIQAGAG GVSGAFKQTP IANWLKNHNQ TENSYQKAVS KFTLSCAGYC 1450 1460 1470 1480 1490 1500 VATYVLGIGD RHNDNIMVDI HGHLFHIDFG HFLGNFKTFA GFQREKAPFV LTPDFVYVIG 1510 1520 1530 1540 1550 1560 GKDSPNFAFF VDICCKAFNI IRSNAHVFIN MFELMLSTGI PELRSENDIV YLRDKFRLDL 1570 1580 1590 1600 1610 1620 TDAEASEYFK KLIHESIGTL TTTINFAIHI MAHRKNLVSG NSAPKIGSAS SLNLNKNKPS 1630 1640 1650 1660 1670 1680 SQSKLDLSRS DLSRSDSSRS DSSRLDLSRS DKKNNKDNKE KEKEKEKEKE KENNDNNDKD 1690 NNNNSNNDTE KENSIDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development." Zhou K., Takegawa K., Emr S.D., Firtel R.A. Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-1697. Strain: AX3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000013 Genomic DNA. Translation: EAL69786.1. U23478 mRNA. Translation: AAA85723.1.
PIR	T18274.
RefSeq	XP_643820.1. XM_638728.1.
3D structure databases
ProteinModelPortal	P54675.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0185203.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185203; DDB0185203; DDB_G0275011.
GeneID	8619867.
KEGG	ddi:DDB_G0275011.
Organism-specific databases
dictyBase	DDB_G0275011. pikC.
Phylogenomic databases
eggNOG	COG5032.
KO	K00922.
OMA	VEIREYA.
ProtClustDB	CLSZ2846827.
Enzyme and pathway databases
BRENDA	2.7.1.137. 1939.
Family and domain databases
Gene3D	1.10.1070.11. 1 hit. 1.25.40.70. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2_dom. IPR000341. PI3K_Ras-bd_dom. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
PANTHER	PTHR10048. PTHR10048. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF00794. PI3K_rbd. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
SMART	SM00142. PI3K_C2. 1 hit. SM00144. PI3K_rbd. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51547. PI3K_C2. 1 hit. PS51546. PI3K_RBD. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI3K3_DICDI

Accession

Primary (citable) accession number: P54675
Secondary accession number(s): Q553Z1, Q869X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)