Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P54674 (PI3K2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 3-kinase 2

Short name=PI3-kinase
Short name=PI3K
Short name=PtdIns-3-kinase
EC=2.7.1.137
Gene names
Name:pikB
Synonyms:pik2
ORF Names:DDB_G0283081
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1857 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 C2 PI3K-type domain.

Contains 1 PI3K-RBD domain.

Contains 1 PI3K/PI4K domain.

Contains 1 PIK helical domain.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 16267269. Source: dictyBase

cell proliferation

Inferred from mutant phenotype PubMed 23843627. Source: dictyBase

cellular component movement

Inferred from genetic interaction PubMed 17462897. Source: dictyBase

chemotaxis

Inferred from mutant phenotype PubMed 16267269. Source: dictyBase

chemotaxis to cAMP

Inferred from genetic interaction PubMed 17535967. Source: dictyBase

macropinocytosis

Inferred from mutant phenotype PubMed 23843627. Source: dictyBase

negative chemotaxis

Inferred from genetic interaction PubMed 17517960. Source: dictyBase

phosphatidylinositol-3-phosphate biosynthetic process

Inferred from Biological aspect of Ancestor. Source: GOC

phosphatidylinositol-mediated signaling

Inferred from electronic annotation. Source: InterPro

regulation of adenylate cyclase activity

Inferred from mutant phenotype PubMed 16267269. Source: dictyBase

regulation of cell morphogenesis

Inferred from genetic interaction PubMed 19011688. Source: dictyBase

regulation of ruffle assembly

Inferred from mutant phenotype PubMed 23843627. Source: dictyBase

response to bacterium

Inferred from mutant phenotype PubMed 21045112. Source: dictyBase

response to cAMP

Inferred from genetic interaction PubMed 16421252. Source: dictyBase

   Cellular_componentcell cortex

Inferred from direct assay PubMed 16968699. Source: dictyBase

cell leading edge

Traceable author statement PubMed 15366706. Source: dictyBase

macropinosome

Inferred from direct assay PubMed 23132928. Source: dictyBase

phosphatidylinositol 3-kinase complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

pseudopodium

Inferred from direct assay PubMed 23132928. Source: dictyBase

   Molecular_function1-phosphatidylinositol-3-kinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

1-phosphatidylinositol-4-phosphate 3-kinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Ras GTPase binding

Inferred from physical interaction PubMed 23843627. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18571857Phosphatidylinositol 3-kinase 2
PRO_0000088823

Regions

Domain821 – 934114PI3K-RBD
Domain1099 – 1271173C2 PI3K-type
Domain1326 – 1503178PIK helical
Domain1596 – 1857262PI3K/PI4K
Compositional bias34 – 407Poly-Gly
Compositional bias166 – 1727Poly-Ser
Compositional bias185 – 22642Poly-Asn
Compositional bias227 – 2359Poly-Thr
Compositional bias246 – 2538Poly-Ser
Compositional bias259 – 26810Poly-Asn
Compositional bias294 – 30310Poly-Thr
Compositional bias361 – 3644Poly-Ser
Compositional bias369 – 38416Poly-Ser
Compositional bias425 – 4295Poly-Gln
Compositional bias439 – 4446Poly-Thr
Compositional bias445 – 45410Poly-Ser
Compositional bias562 – 5709Poly-Gly
Compositional bias715 – 72713Poly-Thr
Compositional bias982 – 9909Poly-Gln
Compositional bias1015 – 104935Poly-Asn

Experimental info

Sequence conflict141S → P in AAA85722. Ref.1
Sequence conflict501S → C in AAA85722. Ref.1
Sequence conflict4971D → V in AAA85722. Ref.1
Sequence conflict10141S → SN in AAA85722. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54674 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 750336008B288443

FASTA1,857203,822
        10         20         30         40         50         60 
MKMSEGIISP LSLSSESSEQ QQAAIRKFSN GSNGSGGGGG SNLSVNSSNS GSNNSIRKSS 

        70         80         90        100        110        120 
TLMYNGPLPS INDGKELLLE NSKPKVVELV NTFNHKPLST IHSVHNEIPP PAIEKEKKEI 

       130        140        150        160        170        180 
INTISNSGVT KYMTALEILD STINTPLNRS RSGSIGSKPI CNNLTSSSSS SSTTATTPSP 

       190        200        210        220        230        240 
TTTSNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNTTST TTTTTSILIS 

       250        260        270        280        290        300 
SSPPPSSSSS SSSNDEQFNN NNNNNNSNSG GSSRMITSKS QIKPLIVTSN TAATTTTTTT 

       310        320        330        340        350        360 
TNTSAPTTPT NRVQSSLDDL LFNLPTIPSN VPTVNGGPKI SAVPKKVSSS KLLIPPSSNV 

       370        380        390        400        410        420 
SSSSNITLSL SSSSPSSSSS SSTSTVVPIV QLSSSNSTNS PSTSLPTTPR LSQPTTSYTQ 

       430        440        450        460        470        480 
LIPSQQQQQP TESNSSSNTN TTTTSSSSSS SSSSLTISSP QPSNNSIRIS AFGRSSTQFT 

       490        500        510        520        530        540 
ISSNGIPSSP GQVSNKDYNN IGNLSNSSGE RVKNKQYSML NISKKTILDE SDISSSPRSI 

       550        560        570        580        590        600 
GSPNSIRASI SSQLPPSLSS IGGGGGGGSG PNVVSNKPLV VKKPSTSEHI KKENIWRQTM 

       610        620        630        640        650        660 
IPLTKEDHIK VVFEGIPGKR VIQKFLIDKT PIEIKSKFFE DLKDGDLLNG LTQLPSLIPE 

       670        680        690        700        710        720 
HYELKVLSVN STISNETLPL RRQTLMQACN ISRLFPKLHL ILKSESTTIL DGASTTTTTT 

       730        740        750        760        770        780 
TTTTTTTANQ SSNIITKSNS SLDLTINNSN EIIDVKGHIQ AELEIFELIG TSFTRVLDQG 

       790        800        810        820        830        840 
QEVVSFRRDF AQFRLSNFTS TRNDLSQMIY VSSEPLPLTI PNKITIMVLL PGDGKIIKRV 

       850        860        870        880        890        900 
DCCPNSSVGD VKKEIFKKFA MIDRVHTQGK TQDDFVLKVT GFREYILCIH ELGNLTSRQR 

       910        920        930        940        950        960 
FYPTGSGGDF SLMDYDYIRQ CVGKNQTVEL SLTNNSILSL NQVSEKVSFI DKILETSDFD 

       970        980        990       1000       1010       1020 
DYDEDLDSIN SNSFDDLKQS IQQQQQQQIQ TVINIKETNK ENKDSNKENK DSSSNNNNNN 

      1030       1040       1050       1060       1070       1080 
NNNNNNNNNN NNNNNNNNNN NGNNNGNNSN NNSNSNISRG SIDSEGNGSG SGNGSEQPTL 

      1090       1100       1110       1120       1130       1140 
IGVQNFSLPN NSKLPINIVK RLFRVNIAGL RNLNFNNNED ARNKFADGKN NQPNVFVMAE 

      1150       1160       1170       1180       1190       1200 
LYYGGELLTN PVFTPIAQLA SYGDGSVEFP NWEKGIAFTI PIRYLPRAAR ASFTVYVTTI 

      1210       1220       1230       1240       1250       1260 
SEALESQMDE VVSKSIPIGW SNCLLMNHKG MLRMGPTAFR LWDDGRRANP IGTCVDNQAA 

      1270       1280       1290       1300       1310       1320 
KQPIILLVEF ESFIRPIVYV DTALQSMMVN DSSSISSNGV ESPSIVSFSS SAASSSPLPS 

      1330       1340       1350       1360       1370       1380 
SPLPSPVGLK KLDLDEARRL KALMDSDPLV QLSAEDKKLV YGYRHIYKSK PKALAKFLLS 

      1390       1400       1410       1420       1430       1440 
VNWIDPDQVT DAYRQMNDWA LLKPVQALEI LDAKFADEHV RNFAIKIINS FSDAEFSDFL 

      1450       1460       1470       1480       1490       1500 
LQLTQVLKYE PYHNSDLTHI LIQRALSNRS RIGHFFFWFL KSEMHTPEIE ERYGLLLEGY 

      1510       1520       1530       1540       1550       1560 
LRSCGTHRQD LIKQNQVLKS LHTVAMAVKQ TNGSSERKKV LMEGLSKIKF PDTFQLPLDP 

      1570       1580       1590       1600       1610       1620 
RWEAKGLIID KCRYMDSKKL PLWLVFENVE PHAKPLTVIF KVGDDLRQDI LTLQVLRIMD 

      1630       1640       1650       1660       1670       1680 
KFWKNSGMDL RLQPYKCIAT GDGIGMLEVV LNANTIANIN KDAGGTGALL EEKTLVNWLK 

      1690       1700       1710       1720       1730       1740 
ECNKTEAEYN KAVETFILSC AGYVVATYVM GIGDRHSDNI MITKLGHLFH IDFGHFLGNY 

      1750       1760       1770       1780       1790       1800 
KKKYGFKRER APFIFTPQYM AIVGGKDSEN FKRFVTTCCS AYNILRKNTD LFINLFQLML 

      1810       1820       1830       1840       1850 
STGIPELQVA EDIDYLRKAL APGLSDEEAA EEFTKNISVA LNTKTVLLND IFHGWAH 

« Hide

References

« Hide 'large scale' references
[1]"A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development."
Zhou K., Takegawa K., Emr S.D., Firtel R.A.
Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U23477 mRNA. Translation: AAA85722.1.
AAFI02000050 Genomic DNA. Translation: EAL65869.1.
PIRT18273.
RefSeqXP_639238.1. XM_634146.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0191474.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0191474; DDB0191474; DDB_G0283081.
GeneID8623924.
KEGGddi:DDB_G0283081.

Organism-specific databases

dictyBaseDDB_G0283081. pikB.

Phylogenomic databases

eggNOGCOG5032.
OMALTHILIQ.

Enzyme and pathway databases

BRENDA2.7.1.137. 1939.

Family and domain databases

Gene3D1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERPTHR10048. PTHR10048. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePI3K2_DICDI
AccessionPrimary (citable) accession number: P54674
Secondary accession number(s): Q54RJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase