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Protein

Phosphatidylinositol 3-kinase 2

Gene

pikB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: dictyBase
  • activation of protein kinase activity Source: dictyBase
  • asexual reproduction Source: dictyBase
  • cell migration Source: GO_Central
  • cell motility Source: dictyBase
  • chemotaxis Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • G-protein coupled receptor signaling pathway Source: dictyBase
  • macropinocytosis Source: dictyBase
  • maintenance of cell polarity Source: dictyBase
  • negative chemotaxis Source: dictyBase
  • phagolysosome assembly Source: dictyBase
  • phagosome maturation Source: dictyBase
  • phosphatidylinositol 3-kinase signaling Source: dictyBase
  • phosphatidylinositol biosynthetic process Source: dictyBase
  • phosphatidylinositol-mediated signaling Source: dictyBase
  • positive regulation of macropinocytosis Source: dictyBase
  • pseudopodium assembly Source: dictyBase
  • regulation of adenylate cyclase activity Source: dictyBase
  • regulation of cell morphogenesis Source: dictyBase
  • regulation of chemotaxis Source: dictyBase
  • regulation of ruffle assembly Source: dictyBase
  • response to bacterium Source: dictyBase
  • response to cAMP Source: dictyBase

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137 1939

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase 2 (EC:2.7.1.137)
Short name:
PI3-kinase
Short name:
PI3K
Short name:
PtdIns-3-kinase
Gene namesi
Name:pikB
Synonyms:pik2
ORF Names:DDB_G0283081
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283081 pikB

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: dictyBase
  • cell leading edge Source: dictyBase
  • macropinosome Source: dictyBase
  • phosphatidylinositol 3-kinase complex Source: GO_Central
  • plasma membrane Source: dictyBase
  • pseudopodium Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000888231 – 1857Phosphatidylinositol 3-kinase 2Add BLAST1857

Proteomic databases

PaxDbiP54674
PRIDEiP54674

Interactioni

GO - Molecular functioni

  • Ras GTPase binding Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0191474

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini821 – 934PI3K-RBDPROSITE-ProRule annotationAdd BLAST114
Domaini1099 – 1271C2 PI3K-typePROSITE-ProRule annotationAdd BLAST173
Domaini1326 – 1503PIK helicalPROSITE-ProRule annotationAdd BLAST178
Domaini1596 – 1857PI3K/PI4KPROSITE-ProRule annotationAdd BLAST262

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 40Poly-Gly7
Compositional biasi166 – 172Poly-Ser7
Compositional biasi185 – 226Poly-AsnAdd BLAST42
Compositional biasi227 – 235Poly-Thr9
Compositional biasi246 – 253Poly-Ser8
Compositional biasi259 – 268Poly-Asn10
Compositional biasi294 – 303Poly-Thr10
Compositional biasi361 – 364Poly-Ser4
Compositional biasi369 – 384Poly-SerAdd BLAST16
Compositional biasi425 – 429Poly-Gln5
Compositional biasi439 – 444Poly-Thr6
Compositional biasi445 – 454Poly-Ser10
Compositional biasi562 – 570Poly-Gly9
Compositional biasi715 – 727Poly-ThrAdd BLAST13
Compositional biasi982 – 990Poly-Gln9
Compositional biasi1015 – 1049Poly-AsnAdd BLAST35

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0904 Eukaryota
COG5032 LUCA
InParanoidiP54674
KOiK00922
OMAiLTHILIQ

Family and domain databases

CDDicd00891 PI3Kc, 1 hit
Gene3Di1.10.1070.11, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR035892 C2_domain_sf
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR002420 PI3K_C2_dom
IPR000341 PI3K_Ras-bd_dom
IPR035448 PI3Kc
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10048 PTHR10048, 2 hits
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
PF00792 PI3K_C2, 1 hit
PF00794 PI3K_rbd, 1 hit
PF00613 PI3Ka, 1 hit
SMARTiView protein in SMART
SM00142 PI3K_C2, 1 hit
SM00144 PI3K_rbd, 1 hit
SM00145 PI3Ka, 1 hit
SM00146 PI3Kc, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51547 PI3K_C2, 1 hit
PS51546 PI3K_RBD, 1 hit
PS51545 PIK_HELICAL, 1 hit

Sequencei

Sequence statusi: Complete.

P54674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMSEGIISP LSLSSESSEQ QQAAIRKFSN GSNGSGGGGG SNLSVNSSNS
60 70 80 90 100
GSNNSIRKSS TLMYNGPLPS INDGKELLLE NSKPKVVELV NTFNHKPLST
110 120 130 140 150
IHSVHNEIPP PAIEKEKKEI INTISNSGVT KYMTALEILD STINTPLNRS
160 170 180 190 200
RSGSIGSKPI CNNLTSSSSS SSTTATTPSP TTTSNNNNNN NNNNNNNNNN
210 220 230 240 250
NNNNNNNNNN NNNNNNNNNN NNNNNNTTST TTTTTSILIS SSPPPSSSSS
260 270 280 290 300
SSSNDEQFNN NNNNNNSNSG GSSRMITSKS QIKPLIVTSN TAATTTTTTT
310 320 330 340 350
TNTSAPTTPT NRVQSSLDDL LFNLPTIPSN VPTVNGGPKI SAVPKKVSSS
360 370 380 390 400
KLLIPPSSNV SSSSNITLSL SSSSPSSSSS SSTSTVVPIV QLSSSNSTNS
410 420 430 440 450
PSTSLPTTPR LSQPTTSYTQ LIPSQQQQQP TESNSSSNTN TTTTSSSSSS
460 470 480 490 500
SSSSLTISSP QPSNNSIRIS AFGRSSTQFT ISSNGIPSSP GQVSNKDYNN
510 520 530 540 550
IGNLSNSSGE RVKNKQYSML NISKKTILDE SDISSSPRSI GSPNSIRASI
560 570 580 590 600
SSQLPPSLSS IGGGGGGGSG PNVVSNKPLV VKKPSTSEHI KKENIWRQTM
610 620 630 640 650
IPLTKEDHIK VVFEGIPGKR VIQKFLIDKT PIEIKSKFFE DLKDGDLLNG
660 670 680 690 700
LTQLPSLIPE HYELKVLSVN STISNETLPL RRQTLMQACN ISRLFPKLHL
710 720 730 740 750
ILKSESTTIL DGASTTTTTT TTTTTTTANQ SSNIITKSNS SLDLTINNSN
760 770 780 790 800
EIIDVKGHIQ AELEIFELIG TSFTRVLDQG QEVVSFRRDF AQFRLSNFTS
810 820 830 840 850
TRNDLSQMIY VSSEPLPLTI PNKITIMVLL PGDGKIIKRV DCCPNSSVGD
860 870 880 890 900
VKKEIFKKFA MIDRVHTQGK TQDDFVLKVT GFREYILCIH ELGNLTSRQR
910 920 930 940 950
FYPTGSGGDF SLMDYDYIRQ CVGKNQTVEL SLTNNSILSL NQVSEKVSFI
960 970 980 990 1000
DKILETSDFD DYDEDLDSIN SNSFDDLKQS IQQQQQQQIQ TVINIKETNK
1010 1020 1030 1040 1050
ENKDSNKENK DSSSNNNNNN NNNNNNNNNN NNNNNNNNNN NGNNNGNNSN
1060 1070 1080 1090 1100
NNSNSNISRG SIDSEGNGSG SGNGSEQPTL IGVQNFSLPN NSKLPINIVK
1110 1120 1130 1140 1150
RLFRVNIAGL RNLNFNNNED ARNKFADGKN NQPNVFVMAE LYYGGELLTN
1160 1170 1180 1190 1200
PVFTPIAQLA SYGDGSVEFP NWEKGIAFTI PIRYLPRAAR ASFTVYVTTI
1210 1220 1230 1240 1250
SEALESQMDE VVSKSIPIGW SNCLLMNHKG MLRMGPTAFR LWDDGRRANP
1260 1270 1280 1290 1300
IGTCVDNQAA KQPIILLVEF ESFIRPIVYV DTALQSMMVN DSSSISSNGV
1310 1320 1330 1340 1350
ESPSIVSFSS SAASSSPLPS SPLPSPVGLK KLDLDEARRL KALMDSDPLV
1360 1370 1380 1390 1400
QLSAEDKKLV YGYRHIYKSK PKALAKFLLS VNWIDPDQVT DAYRQMNDWA
1410 1420 1430 1440 1450
LLKPVQALEI LDAKFADEHV RNFAIKIINS FSDAEFSDFL LQLTQVLKYE
1460 1470 1480 1490 1500
PYHNSDLTHI LIQRALSNRS RIGHFFFWFL KSEMHTPEIE ERYGLLLEGY
1510 1520 1530 1540 1550
LRSCGTHRQD LIKQNQVLKS LHTVAMAVKQ TNGSSERKKV LMEGLSKIKF
1560 1570 1580 1590 1600
PDTFQLPLDP RWEAKGLIID KCRYMDSKKL PLWLVFENVE PHAKPLTVIF
1610 1620 1630 1640 1650
KVGDDLRQDI LTLQVLRIMD KFWKNSGMDL RLQPYKCIAT GDGIGMLEVV
1660 1670 1680 1690 1700
LNANTIANIN KDAGGTGALL EEKTLVNWLK ECNKTEAEYN KAVETFILSC
1710 1720 1730 1740 1750
AGYVVATYVM GIGDRHSDNI MITKLGHLFH IDFGHFLGNY KKKYGFKRER
1760 1770 1780 1790 1800
APFIFTPQYM AIVGGKDSEN FKRFVTTCCS AYNILRKNTD LFINLFQLML
1810 1820 1830 1840 1850
STGIPELQVA EDIDYLRKAL APGLSDEEAA EEFTKNISVA LNTKTVLLND

IFHGWAH
Length:1,857
Mass (Da):203,822
Last modified:December 4, 2007 - v2
Checksum:i750336008B288443
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14S → P in AAA85722 (PubMed:7565716).Curated1
Sequence conflicti50S → C in AAA85722 (PubMed:7565716).Curated1
Sequence conflicti497D → V in AAA85722 (PubMed:7565716).Curated1
Sequence conflicti1014S → SN in AAA85722 (PubMed:7565716).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23477 mRNA Translation: AAA85722.1
AAFI02000050 Genomic DNA Translation: EAL65869.1
PIRiT18273
RefSeqiXP_639238.1, XM_634146.1

Genome annotation databases

EnsemblProtistsiEAL65869; EAL65869; DDB_G0283081
GeneIDi8623924
KEGGiddi:DDB_G0283081

Entry informationi

Entry nameiPI3K2_DICDI
AccessioniPrimary (citable) accession number: P54674
Secondary accession number(s): Q54RJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: May 23, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

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