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Protein

Phosphatidylinositol 3-kinase 2

Gene

pikB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: dictyBase
  • activation of protein kinase activity Source: dictyBase
  • asexual reproduction Source: dictyBase
  • cell motility Source: dictyBase
  • chemotaxis Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • G-protein coupled receptor signaling pathway Source: dictyBase
  • macropinocytosis Source: dictyBase
  • maintenance of cell polarity Source: dictyBase
  • negative chemotaxis Source: dictyBase
  • phosphatidylinositol 3-kinase signaling Source: dictyBase
  • phosphatidylinositol biosynthetic process Source: dictyBase
  • phosphatidylinositol-mediated signaling Source: dictyBase
  • pseudopodium assembly Source: dictyBase
  • regulation of adenylate cyclase activity Source: dictyBase
  • regulation of cell morphogenesis Source: dictyBase
  • regulation of chemotaxis Source: dictyBase
  • regulation of ruffle assembly Source: dictyBase
  • response to bacterium Source: dictyBase
  • response to cAMP Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase 2 (EC:2.7.1.137)
Short name:
PI3-kinase
Short name:
PI3K
Short name:
PtdIns-3-kinase
Gene namesi
Name:pikB
Synonyms:pik2
ORF Names:DDB_G0283081
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283081. pikB.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: dictyBase
  • cell leading edge Source: dictyBase
  • macropinosome Source: dictyBase
  • phosphatidylinositol 3-kinase complex Source: GO_Central
  • plasma membrane Source: dictyBase
  • pseudopodium Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000888231 – 1857Phosphatidylinositol 3-kinase 2Add BLAST1857

Proteomic databases

PaxDbiP54674.

Interactioni

GO - Molecular functioni

  • Ras GTPase binding Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0191474.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini821 – 934PI3K-RBDPROSITE-ProRule annotationAdd BLAST114
Domaini1099 – 1271C2 PI3K-typePROSITE-ProRule annotationAdd BLAST173
Domaini1326 – 1503PIK helicalPROSITE-ProRule annotationAdd BLAST178
Domaini1596 – 1857PI3K/PI4KPROSITE-ProRule annotationAdd BLAST262

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 40Poly-Gly7
Compositional biasi166 – 172Poly-Ser7
Compositional biasi185 – 226Poly-AsnAdd BLAST42
Compositional biasi227 – 235Poly-Thr9
Compositional biasi246 – 253Poly-Ser8
Compositional biasi259 – 268Poly-Asn10
Compositional biasi294 – 303Poly-Thr10
Compositional biasi361 – 364Poly-Ser4
Compositional biasi369 – 384Poly-SerAdd BLAST16
Compositional biasi425 – 429Poly-Gln5
Compositional biasi439 – 444Poly-Thr6
Compositional biasi445 – 454Poly-Ser10
Compositional biasi562 – 570Poly-Gly9
Compositional biasi715 – 727Poly-ThrAdd BLAST13
Compositional biasi982 – 990Poly-Gln9
Compositional biasi1015 – 1049Poly-AsnAdd BLAST35

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K-RBD domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0904. Eukaryota.
COG5032. LUCA.
InParanoidiP54674.
OMAiLTHILIQ.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 5 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMSEGIISP LSLSSESSEQ QQAAIRKFSN GSNGSGGGGG SNLSVNSSNS
60 70 80 90 100
GSNNSIRKSS TLMYNGPLPS INDGKELLLE NSKPKVVELV NTFNHKPLST
110 120 130 140 150
IHSVHNEIPP PAIEKEKKEI INTISNSGVT KYMTALEILD STINTPLNRS
160 170 180 190 200
RSGSIGSKPI CNNLTSSSSS SSTTATTPSP TTTSNNNNNN NNNNNNNNNN
210 220 230 240 250
NNNNNNNNNN NNNNNNNNNN NNNNNNTTST TTTTTSILIS SSPPPSSSSS
260 270 280 290 300
SSSNDEQFNN NNNNNNSNSG GSSRMITSKS QIKPLIVTSN TAATTTTTTT
310 320 330 340 350
TNTSAPTTPT NRVQSSLDDL LFNLPTIPSN VPTVNGGPKI SAVPKKVSSS
360 370 380 390 400
KLLIPPSSNV SSSSNITLSL SSSSPSSSSS SSTSTVVPIV QLSSSNSTNS
410 420 430 440 450
PSTSLPTTPR LSQPTTSYTQ LIPSQQQQQP TESNSSSNTN TTTTSSSSSS
460 470 480 490 500
SSSSLTISSP QPSNNSIRIS AFGRSSTQFT ISSNGIPSSP GQVSNKDYNN
510 520 530 540 550
IGNLSNSSGE RVKNKQYSML NISKKTILDE SDISSSPRSI GSPNSIRASI
560 570 580 590 600
SSQLPPSLSS IGGGGGGGSG PNVVSNKPLV VKKPSTSEHI KKENIWRQTM
610 620 630 640 650
IPLTKEDHIK VVFEGIPGKR VIQKFLIDKT PIEIKSKFFE DLKDGDLLNG
660 670 680 690 700
LTQLPSLIPE HYELKVLSVN STISNETLPL RRQTLMQACN ISRLFPKLHL
710 720 730 740 750
ILKSESTTIL DGASTTTTTT TTTTTTTANQ SSNIITKSNS SLDLTINNSN
760 770 780 790 800
EIIDVKGHIQ AELEIFELIG TSFTRVLDQG QEVVSFRRDF AQFRLSNFTS
810 820 830 840 850
TRNDLSQMIY VSSEPLPLTI PNKITIMVLL PGDGKIIKRV DCCPNSSVGD
860 870 880 890 900
VKKEIFKKFA MIDRVHTQGK TQDDFVLKVT GFREYILCIH ELGNLTSRQR
910 920 930 940 950
FYPTGSGGDF SLMDYDYIRQ CVGKNQTVEL SLTNNSILSL NQVSEKVSFI
960 970 980 990 1000
DKILETSDFD DYDEDLDSIN SNSFDDLKQS IQQQQQQQIQ TVINIKETNK
1010 1020 1030 1040 1050
ENKDSNKENK DSSSNNNNNN NNNNNNNNNN NNNNNNNNNN NGNNNGNNSN
1060 1070 1080 1090 1100
NNSNSNISRG SIDSEGNGSG SGNGSEQPTL IGVQNFSLPN NSKLPINIVK
1110 1120 1130 1140 1150
RLFRVNIAGL RNLNFNNNED ARNKFADGKN NQPNVFVMAE LYYGGELLTN
1160 1170 1180 1190 1200
PVFTPIAQLA SYGDGSVEFP NWEKGIAFTI PIRYLPRAAR ASFTVYVTTI
1210 1220 1230 1240 1250
SEALESQMDE VVSKSIPIGW SNCLLMNHKG MLRMGPTAFR LWDDGRRANP
1260 1270 1280 1290 1300
IGTCVDNQAA KQPIILLVEF ESFIRPIVYV DTALQSMMVN DSSSISSNGV
1310 1320 1330 1340 1350
ESPSIVSFSS SAASSSPLPS SPLPSPVGLK KLDLDEARRL KALMDSDPLV
1360 1370 1380 1390 1400
QLSAEDKKLV YGYRHIYKSK PKALAKFLLS VNWIDPDQVT DAYRQMNDWA
1410 1420 1430 1440 1450
LLKPVQALEI LDAKFADEHV RNFAIKIINS FSDAEFSDFL LQLTQVLKYE
1460 1470 1480 1490 1500
PYHNSDLTHI LIQRALSNRS RIGHFFFWFL KSEMHTPEIE ERYGLLLEGY
1510 1520 1530 1540 1550
LRSCGTHRQD LIKQNQVLKS LHTVAMAVKQ TNGSSERKKV LMEGLSKIKF
1560 1570 1580 1590 1600
PDTFQLPLDP RWEAKGLIID KCRYMDSKKL PLWLVFENVE PHAKPLTVIF
1610 1620 1630 1640 1650
KVGDDLRQDI LTLQVLRIMD KFWKNSGMDL RLQPYKCIAT GDGIGMLEVV
1660 1670 1680 1690 1700
LNANTIANIN KDAGGTGALL EEKTLVNWLK ECNKTEAEYN KAVETFILSC
1710 1720 1730 1740 1750
AGYVVATYVM GIGDRHSDNI MITKLGHLFH IDFGHFLGNY KKKYGFKRER
1760 1770 1780 1790 1800
APFIFTPQYM AIVGGKDSEN FKRFVTTCCS AYNILRKNTD LFINLFQLML
1810 1820 1830 1840 1850
STGIPELQVA EDIDYLRKAL APGLSDEEAA EEFTKNISVA LNTKTVLLND

IFHGWAH
Length:1,857
Mass (Da):203,822
Last modified:December 4, 2007 - v2
Checksum:i750336008B288443
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14S → P in AAA85722 (PubMed:7565716).Curated1
Sequence conflicti50S → C in AAA85722 (PubMed:7565716).Curated1
Sequence conflicti497D → V in AAA85722 (PubMed:7565716).Curated1
Sequence conflicti1014S → SN in AAA85722 (PubMed:7565716).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23477 mRNA. Translation: AAA85722.1.
AAFI02000050 Genomic DNA. Translation: EAL65869.1.
PIRiT18273.
RefSeqiXP_639238.1. XM_634146.1.

Genome annotation databases

EnsemblProtistsiEAL65869; EAL65869; DDB_G0283081.
GeneIDi8623924.
KEGGiddi:DDB_G0283081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23477 mRNA. Translation: AAA85722.1.
AAFI02000050 Genomic DNA. Translation: EAL65869.1.
PIRiT18273.
RefSeqiXP_639238.1. XM_634146.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191474.

Proteomic databases

PaxDbiP54674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL65869; EAL65869; DDB_G0283081.
GeneIDi8623924.
KEGGiddi:DDB_G0283081.

Organism-specific databases

dictyBaseiDDB_G0283081. pikB.

Phylogenomic databases

eggNOGiKOG0904. Eukaryota.
COG5032. LUCA.
InParanoidiP54674.
OMAiLTHILIQ.

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.

Miscellaneous databases

PROiP54674.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 5 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPI3K2_DICDI
AccessioniPrimary (citable) accession number: P54674
Secondary accession number(s): Q54RJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: November 30, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.