SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P54674

- PI3K2_DICDI

UniProt

P54674 - PI3K2_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Phosphatidylinositol 3-kinase 2
Gene
pikB, pik2, DDB_G0283081
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

  1. 1-phosphatidylinositol-3-kinase activity Source: RefGenome
  2. 1-phosphatidylinositol-4-phosphate 3-kinase activity Source: RefGenome
  3. ATP binding Source: UniProtKB-KW
  4. Ras GTPase binding Source: dictyBase

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: dictyBase
  2. cell proliferation Source: dictyBase
  3. cellular component movement Source: dictyBase
  4. chemotaxis Source: dictyBase
  5. chemotaxis to cAMP Source: dictyBase
  6. macropinocytosis Source: dictyBase
  7. negative chemotaxis Source: dictyBase
  8. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
  9. phosphatidylinositol-mediated signaling Source: InterPro
  10. regulation of adenylate cyclase activity Source: dictyBase
  11. regulation of cell morphogenesis Source: dictyBase
  12. regulation of ruffle assembly Source: dictyBase
  13. response to bacterium Source: dictyBase
  14. response to cAMP Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase 2 (EC:2.7.1.137)
Short name:
PI3-kinase
Short name:
PI3K
Short name:
PtdIns-3-kinase
Gene namesi
Name:pikB
Synonyms:pik2
ORF Names:DDB_G0283081
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0283081. pikB.

Subcellular locationi

GO - Cellular componenti

  1. cell cortex Source: dictyBase
  2. cell leading edge Source: dictyBase
  3. macropinosome Source: dictyBase
  4. phosphatidylinositol 3-kinase complex Source: RefGenome
  5. plasma membrane Source: RefGenome
  6. pseudopodium Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18571857Phosphatidylinositol 3-kinase 2
PRO_0000088823Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0191474.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini821 – 934114PI3K-RBD
Add
BLAST
Domaini1099 – 1271173C2 PI3K-type
Add
BLAST
Domaini1326 – 1503178PIK helical
Add
BLAST
Domaini1596 – 1857262PI3K/PI4K
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi34 – 407Poly-Gly
Compositional biasi166 – 1727Poly-Ser
Compositional biasi185 – 22642Poly-Asn
Add
BLAST
Compositional biasi227 – 2359Poly-Thr
Compositional biasi246 – 2538Poly-Ser
Compositional biasi259 – 26810Poly-Asn
Compositional biasi294 – 30310Poly-Thr
Compositional biasi361 – 3644Poly-Ser
Compositional biasi369 – 38416Poly-Ser
Add
BLAST
Compositional biasi425 – 4295Poly-Gln
Compositional biasi439 – 4446Poly-Thr
Compositional biasi445 – 45410Poly-Ser
Compositional biasi562 – 5709Poly-Gly
Compositional biasi715 – 72713Poly-Thr
Add
BLAST
Compositional biasi982 – 9909Poly-Gln
Compositional biasi1015 – 104935Poly-Asn
Add
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K-RBD domain.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.

Phylogenomic databases

eggNOGiCOG5032.
OMAiLTHILIQ.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54674-1 [UniParc]FASTAAdd to Basket

« Hide

MKMSEGIISP LSLSSESSEQ QQAAIRKFSN GSNGSGGGGG SNLSVNSSNS     50
GSNNSIRKSS TLMYNGPLPS INDGKELLLE NSKPKVVELV NTFNHKPLST 100
IHSVHNEIPP PAIEKEKKEI INTISNSGVT KYMTALEILD STINTPLNRS 150
RSGSIGSKPI CNNLTSSSSS SSTTATTPSP TTTSNNNNNN NNNNNNNNNN 200
NNNNNNNNNN NNNNNNNNNN NNNNNNTTST TTTTTSILIS SSPPPSSSSS 250
SSSNDEQFNN NNNNNNSNSG GSSRMITSKS QIKPLIVTSN TAATTTTTTT 300
TNTSAPTTPT NRVQSSLDDL LFNLPTIPSN VPTVNGGPKI SAVPKKVSSS 350
KLLIPPSSNV SSSSNITLSL SSSSPSSSSS SSTSTVVPIV QLSSSNSTNS 400
PSTSLPTTPR LSQPTTSYTQ LIPSQQQQQP TESNSSSNTN TTTTSSSSSS 450
SSSSLTISSP QPSNNSIRIS AFGRSSTQFT ISSNGIPSSP GQVSNKDYNN 500
IGNLSNSSGE RVKNKQYSML NISKKTILDE SDISSSPRSI GSPNSIRASI 550
SSQLPPSLSS IGGGGGGGSG PNVVSNKPLV VKKPSTSEHI KKENIWRQTM 600
IPLTKEDHIK VVFEGIPGKR VIQKFLIDKT PIEIKSKFFE DLKDGDLLNG 650
LTQLPSLIPE HYELKVLSVN STISNETLPL RRQTLMQACN ISRLFPKLHL 700
ILKSESTTIL DGASTTTTTT TTTTTTTANQ SSNIITKSNS SLDLTINNSN 750
EIIDVKGHIQ AELEIFELIG TSFTRVLDQG QEVVSFRRDF AQFRLSNFTS 800
TRNDLSQMIY VSSEPLPLTI PNKITIMVLL PGDGKIIKRV DCCPNSSVGD 850
VKKEIFKKFA MIDRVHTQGK TQDDFVLKVT GFREYILCIH ELGNLTSRQR 900
FYPTGSGGDF SLMDYDYIRQ CVGKNQTVEL SLTNNSILSL NQVSEKVSFI 950
DKILETSDFD DYDEDLDSIN SNSFDDLKQS IQQQQQQQIQ TVINIKETNK 1000
ENKDSNKENK DSSSNNNNNN NNNNNNNNNN NNNNNNNNNN NGNNNGNNSN 1050
NNSNSNISRG SIDSEGNGSG SGNGSEQPTL IGVQNFSLPN NSKLPINIVK 1100
RLFRVNIAGL RNLNFNNNED ARNKFADGKN NQPNVFVMAE LYYGGELLTN 1150
PVFTPIAQLA SYGDGSVEFP NWEKGIAFTI PIRYLPRAAR ASFTVYVTTI 1200
SEALESQMDE VVSKSIPIGW SNCLLMNHKG MLRMGPTAFR LWDDGRRANP 1250
IGTCVDNQAA KQPIILLVEF ESFIRPIVYV DTALQSMMVN DSSSISSNGV 1300
ESPSIVSFSS SAASSSPLPS SPLPSPVGLK KLDLDEARRL KALMDSDPLV 1350
QLSAEDKKLV YGYRHIYKSK PKALAKFLLS VNWIDPDQVT DAYRQMNDWA 1400
LLKPVQALEI LDAKFADEHV RNFAIKIINS FSDAEFSDFL LQLTQVLKYE 1450
PYHNSDLTHI LIQRALSNRS RIGHFFFWFL KSEMHTPEIE ERYGLLLEGY 1500
LRSCGTHRQD LIKQNQVLKS LHTVAMAVKQ TNGSSERKKV LMEGLSKIKF 1550
PDTFQLPLDP RWEAKGLIID KCRYMDSKKL PLWLVFENVE PHAKPLTVIF 1600
KVGDDLRQDI LTLQVLRIMD KFWKNSGMDL RLQPYKCIAT GDGIGMLEVV 1650
LNANTIANIN KDAGGTGALL EEKTLVNWLK ECNKTEAEYN KAVETFILSC 1700
AGYVVATYVM GIGDRHSDNI MITKLGHLFH IDFGHFLGNY KKKYGFKRER 1750
APFIFTPQYM AIVGGKDSEN FKRFVTTCCS AYNILRKNTD LFINLFQLML 1800
STGIPELQVA EDIDYLRKAL APGLSDEEAA EEFTKNISVA LNTKTVLLND 1850
IFHGWAH 1857
Length:1,857
Mass (Da):203,822
Last modified:December 4, 2007 - v2
Checksum:i750336008B288443
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141S → P in AAA85722. 1 Publication
Sequence conflicti50 – 501S → C in AAA85722. 1 Publication
Sequence conflicti497 – 4971D → V in AAA85722. 1 Publication
Sequence conflicti1014 – 10141S → SN in AAA85722. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23477 mRNA. Translation: AAA85722.1.
AAFI02000050 Genomic DNA. Translation: EAL65869.1.
PIRiT18273.
RefSeqiXP_639238.1. XM_634146.1.

Genome annotation databases

EnsemblProtistsiDDB0191474; DDB0191474; DDB_G0283081.
GeneIDi8623924.
KEGGiddi:DDB_G0283081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23477 mRNA. Translation: AAA85722.1 .
AAFI02000050 Genomic DNA. Translation: EAL65869.1 .
PIRi T18273.
RefSeqi XP_639238.1. XM_634146.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191474.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191474 ; DDB0191474 ; DDB_G0283081 .
GeneIDi 8623924.
KEGGi ddi:DDB_G0283081.

Organism-specific databases

dictyBasei DDB_G0283081. pikB.

Phylogenomic databases

eggNOGi COG5032.
OMAi LTHILIQ.

Enzyme and pathway databases

BRENDAi 2.7.1.137. 1939.

Family and domain databases

Gene3Di 1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProi IPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
PANTHERi PTHR10048. PTHR10048. 1 hit.
Pfami PF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view ]
SMARTi SM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development."
    Zhou K., Takegawa K., Emr S.D., Firtel R.A.
    Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPI3K2_DICDI
AccessioniPrimary (citable) accession number: P54674
Secondary accession number(s): Q54RJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3