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Protein

Phosphatidylinositol 3-kinase 1

Gene

pikA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: dictyBase
  • activation of protein kinase activity Source: dictyBase
  • aggregation involved in sorocarp development Source: dictyBase
  • asexual reproduction Source: dictyBase
  • cell motility Source: dictyBase
  • chemotaxis Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • G-protein coupled receptor signaling pathway Source: dictyBase
  • macropinocytosis Source: dictyBase
  • maintenance of cell polarity Source: dictyBase
  • negative chemotaxis Source: dictyBase
  • phosphatidylinositol 3-kinase signaling Source: dictyBase
  • phosphatidylinositol biosynthetic process Source: dictyBase
  • phosphatidylinositol-mediated signaling Source: dictyBase
  • pseudopodium assembly Source: dictyBase
  • regulation of adenylate cyclase activity Source: dictyBase
  • regulation of cell morphogenesis Source: dictyBase
  • regulation of chemotaxis Source: dictyBase
  • regulation of ruffle assembly Source: dictyBase
  • response to bacterium Source: dictyBase
  • response to cAMP Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase 1 (EC:2.7.1.137)
Short name:
PI3-kinase
Short name:
PI3K
Short name:
PtdIns-3-kinase
Gene namesi
Name:pikA
Synonyms:pik1
ORF Names:DDB_G0278727
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278727. pikA.

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: dictyBase
  • intracellular Source: GOC
  • phosphatidylinositol 3-kinase complex Source: GO_Central
  • plasma membrane Source: dictyBase
  • pseudopodium Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000888221 – 1571Phosphatidylinositol 3-kinase 1Add BLAST1571

Proteomic databases

PaxDbiP54673.
PRIDEiP54673.

Interactioni

GO - Molecular functioni

  • Ras GTPase binding Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0214949.

Structurei

3D structure databases

ProteinModelPortaliP54673.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini530 – 627PI3K-ABDPROSITE-ProRule annotationAdd BLAST98
Domaini700 – 789PI3K-RBDPROSITE-ProRule annotationAdd BLAST90
Domaini851 – 1020C2 PI3K-typePROSITE-ProRule annotationAdd BLAST170
Domaini1040 – 1216PIK helicalPROSITE-ProRule annotationAdd BLAST177
Domaini1309 – 1571PI3K/PI4KPROSITE-ProRule annotationAdd BLAST263

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 48Poly-Asn8
Compositional biasi59 – 73Poly-AsnAdd BLAST15
Compositional biasi161 – 168Poly-Asn8
Compositional biasi294 – 304Poly-ThrAdd BLAST11
Compositional biasi308 – 324Poly-ThrAdd BLAST17
Compositional biasi413 – 424Poly-AsnAdd BLAST12
Compositional biasi503 – 510Poly-Ser8
Compositional biasi570 – 579Poly-Asn10
Compositional biasi821 – 828Poly-Asn8
Compositional biasi831 – 836Poly-Asn6

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K-ABD domain.PROSITE-ProRule annotation
Contains 1 PI3K-RBD domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0904. Eukaryota.
COG5032. LUCA.
InParanoidiP54673.
KOiK00922.
OMAiCCTAYNI.
PhylomeDBiP54673.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 2 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSIESSSND SNEINKNSNK NNTHLNSNYN NIYKNNSTSS NNNNNHNNIE
60 70 80 90 100
IIGIDNNKNN NKNNNDNNNN NNNIDKKRKD SKNKQNQEIN QEMSENKKIY
110 120 130 140 150
NSNDSNCSSG SSSGGHVNNG HHILIEENER LEHENQEIQE IYKQKGMEFQ
160 170 180 190 200
KKDLRFGYDV NSNNNNNNGG GSSSGSSSGG SDESASNQPI IRTRNREGSI
210 220 230 240 250
LNLKKQGLVK EISQRFQTPD TASYTRPNAN NISIKDKISI LKKEQERRKQ
260 270 280 290 300
DSEVQQREKV IVLSADSSNI QIYHPSVLIE KMNSKLDTEE KPATTTTTTT
310 320 330 340 350
TTSTSISTST PTTTTTTTTN TSTTNDITIK PKTSPTKNNE ERSQSPITTP
360 370 380 390 400
KQPVEEIVKK VSTPKSNNTS KKTSSDTTPT GKTTKKDKKD KKDKSRDSGN
410 420 430 440 450
LVIVNNTNNT SSNNNNNNNN NNNNETIIKR RGRVLVTPSS DLKKNIQIYF
460 470 480 490 500
TIPINPPVNK TNKPNQLLSN TSQQFLKTLI SNEIPIDCKI NDINDTDAFS
510 520 530 540 550
DLSASASSSS FITKSSQSLL NVQSLRVKAI KTSFNILFLM PNQSKKILQV
560 570 580 590 600
KGSDTIENLK ERIISDYLFN NNSNNNNNNC KYGADSYLIL DFNDNPMERS
610 620 630 640 650
LVLNKSDYIL DKRAQGLIPK LKVIEKSTIL DSDPSDELSP SEYEIIRKLI
660 670 680 690 700
PGTDTWRGEE VEYFRRVTSR LRYEALPLIK GSIQSTLLVR LSPLPIPIVG
710 720 730 740 750
NKILISIFLP ITQVTKTLDL ELNETADQFT NRLFTKNYSK HLPNVNSNDF
760 770 780 790 800
ILKVVGSSDF IHGPHDIRTF ESIRNHIIQG TKPQLTLIQR PKPELDPQPF
810 820 830 840 850
KPRFDYPPEL IIDHSCSNAI NCNNNNTNST NNNNINFDNW DQITHISIRE
860 870 880 890 900
IKKPFRVKVM GSTRIPLSCI KDIDSSSVIV SISLYHGIEC FSKAFTQPII
910 920 930 940 950
PPPFAFLAET LSVDWCEWLV FTNIDYSNLP VDARLSISVY SANETVDDVE
960 970 980 990 1000
EIKNLDEATK KLTPIGWINV MITDFKYQLR QGMVELSLWP SDFSNPLGTC
1010 1020 1030 1040 1050
SNNPSSSQSV GLTLEFEEFN LPVLFPRKTK FSTSVSVIEQ PPTNINSNEM
1060 1070 1080 1090 1100
REFFEQITAL DPLSDLKQEK YNQLWTLRHY SILFPQVLPR LMLSVPWTQA
1110 1120 1130 1140 1150
TAVDEAISLL DRWPKLKPYE SLELLDAKHA NRKVREFAVT CLEDLSEDEL
1160 1170 1180 1190 1200
LDILLQLVQV LKYEPFHDSK LSRFLLRKAI LNRNIGHSFF WYLKSDLHDS
1210 1220 1230 1240 1250
NLSERFGILL ESYLYACGAH RIELLKQMEV INNLTEVAKK IKPLKDQDRR
1260 1270 1280 1290 1300
EFMIKEFESL EWPKRFHLTL NPRFESNGLI INKSKYMDSK KLPLRLSFTN
1310 1320 1330 1340 1350
TDMNADPIEV IFKAGDDLRQ DMLTLQMIRL MDKLWQKEGL DLKLSPYGCI
1360 1370 1380 1390 1400
STGDMIGMIE VVLNSETTAK IQKSEGGGAA SAFKLDPLAN WILQHNKSDM
1410 1420 1430 1440 1450
EYQKAVDTFI LSCAGYCVAT YVLGIGDRHN DNLMVTKGGR LFHIDFGHFL
1460 1470 1480 1490 1500
GNYKKKFGFK RERAPFVFTP DFCYVMGGKE SFKFSQFVNY CCTAYNIVRK
1510 1520 1530 1540 1550
NAKLFMNLFA MMVSTGIPEL QSMEDLNYLK ESFSIELSDE KAREKFVALI
1560 1570
HESLATKTTQ LNNFFHHLAH A
Length:1,571
Mass (Da):178,472
Last modified:December 4, 2007 - v2
Checksum:i9550F58C0E822C9F
GO

Sequence cautioni

The sequence AAA85721 differs from that shown. Reason: Frameshift at positions 1380 and 1391.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti749D → H in AAA85721 (PubMed:7565716).Curated1
Sequence conflicti1494A → G in AAA85721 (PubMed:7565716).Curated1
Sequence conflicti1540E → A in AAA85721 (PubMed:7565716).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23476 mRNA. Translation: AAA85721.1. Frameshift.
AAFI02000024 Genomic DNA. Translation: EAL67965.1.
PIRiT18272.
RefSeqiXP_641955.1. XM_636863.1.

Genome annotation databases

EnsemblProtistsiEAL67965; EAL67965; DDB_G0278727.
GeneIDi8621687.
KEGGiddi:DDB_G0278727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23476 mRNA. Translation: AAA85721.1. Frameshift.
AAFI02000024 Genomic DNA. Translation: EAL67965.1.
PIRiT18272.
RefSeqiXP_641955.1. XM_636863.1.

3D structure databases

ProteinModelPortaliP54673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0214949.

Proteomic databases

PaxDbiP54673.
PRIDEiP54673.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL67965; EAL67965; DDB_G0278727.
GeneIDi8621687.
KEGGiddi:DDB_G0278727.

Organism-specific databases

dictyBaseiDDB_G0278727. pikA.

Phylogenomic databases

eggNOGiKOG0904. Eukaryota.
COG5032. LUCA.
InParanoidiP54673.
KOiK00922.
OMAiCCTAYNI.
PhylomeDBiP54673.

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.

Miscellaneous databases

PROiP54673.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 2 hits.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPI3K1_DICDI
AccessioniPrimary (citable) accession number: P54673
Secondary accession number(s): Q54XS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: November 30, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.