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P54673

- PI3K1_DICDI

UniProt

P54673 - PI3K1_DICDI

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Protein
Phosphatidylinositol 3-kinase 1
Gene
pikA, pik1, DDB_G0278727
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

  1. 1-phosphatidylinositol-3-kinase activity Source: RefGenome
  2. 1-phosphatidylinositol-4-phosphate 3-kinase activity Source: RefGenome
  3. ATP binding Source: UniProtKB-KW
  4. Ras GTPase binding Source: dictyBase

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: dictyBase
  2. aggregation involved in sorocarp development Source: dictyBase
  3. cell proliferation Source: dictyBase
  4. cellular component movement Source: dictyBase
  5. chemotaxis Source: dictyBase
  6. chemotaxis to cAMP Source: dictyBase
  7. macropinocytosis Source: dictyBase
  8. negative chemotaxis Source: dictyBase
  9. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
  10. phosphatidylinositol-mediated signaling Source: dictyBase
  11. regulation of adenylate cyclase activity Source: dictyBase
  12. regulation of cell morphogenesis Source: dictyBase
  13. regulation of ruffle assembly Source: dictyBase
  14. response to bacterium Source: dictyBase
  15. response to cAMP Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase 1 (EC:2.7.1.137)
Short name:
PI3-kinase
Short name:
PI3K
Short name:
PtdIns-3-kinase
Gene namesi
Name:pikA
Synonyms:pik1
ORF Names:DDB_G0278727
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278727. pikA.

Subcellular locationi

GO - Cellular componenti

  1. cell leading edge Source: dictyBase
  2. phosphatidylinositol 3-kinase complex Source: RefGenome
  3. plasma membrane Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15711571Phosphatidylinositol 3-kinase 1
PRO_0000088822Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0214949.

Structurei

3D structure databases

ProteinModelPortaliP54673.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini530 – 62798PI3K-ABD
Add
BLAST
Domaini700 – 78990PI3K-RBD
Add
BLAST
Domaini851 – 1020170C2 PI3K-type
Add
BLAST
Domaini1040 – 1216177PIK helical
Add
BLAST
Domaini1309 – 1571263PI3K/PI4K
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 488Poly-Asn
Compositional biasi59 – 7315Poly-Asn
Add
BLAST
Compositional biasi161 – 1688Poly-Asn
Compositional biasi294 – 30411Poly-Thr
Add
BLAST
Compositional biasi308 – 32417Poly-Thr
Add
BLAST
Compositional biasi413 – 42412Poly-Asn
Add
BLAST
Compositional biasi503 – 5108Poly-Ser
Compositional biasi570 – 57910Poly-Asn
Compositional biasi821 – 8288Poly-Asn
Compositional biasi831 – 8366Poly-Asn

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K-ABD domain.
Contains 1 PI3K-RBD domain.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.

Phylogenomic databases

eggNOGiCOG5032.
KOiK00922.
OMAiDAKHANR.
PhylomeDBiP54673.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54673-1 [UniParc]FASTAAdd to Basket

« Hide

MNSIESSSND SNEINKNSNK NNTHLNSNYN NIYKNNSTSS NNNNNHNNIE     50
IIGIDNNKNN NKNNNDNNNN NNNIDKKRKD SKNKQNQEIN QEMSENKKIY 100
NSNDSNCSSG SSSGGHVNNG HHILIEENER LEHENQEIQE IYKQKGMEFQ 150
KKDLRFGYDV NSNNNNNNGG GSSSGSSSGG SDESASNQPI IRTRNREGSI 200
LNLKKQGLVK EISQRFQTPD TASYTRPNAN NISIKDKISI LKKEQERRKQ 250
DSEVQQREKV IVLSADSSNI QIYHPSVLIE KMNSKLDTEE KPATTTTTTT 300
TTSTSISTST PTTTTTTTTN TSTTNDITIK PKTSPTKNNE ERSQSPITTP 350
KQPVEEIVKK VSTPKSNNTS KKTSSDTTPT GKTTKKDKKD KKDKSRDSGN 400
LVIVNNTNNT SSNNNNNNNN NNNNETIIKR RGRVLVTPSS DLKKNIQIYF 450
TIPINPPVNK TNKPNQLLSN TSQQFLKTLI SNEIPIDCKI NDINDTDAFS 500
DLSASASSSS FITKSSQSLL NVQSLRVKAI KTSFNILFLM PNQSKKILQV 550
KGSDTIENLK ERIISDYLFN NNSNNNNNNC KYGADSYLIL DFNDNPMERS 600
LVLNKSDYIL DKRAQGLIPK LKVIEKSTIL DSDPSDELSP SEYEIIRKLI 650
PGTDTWRGEE VEYFRRVTSR LRYEALPLIK GSIQSTLLVR LSPLPIPIVG 700
NKILISIFLP ITQVTKTLDL ELNETADQFT NRLFTKNYSK HLPNVNSNDF 750
ILKVVGSSDF IHGPHDIRTF ESIRNHIIQG TKPQLTLIQR PKPELDPQPF 800
KPRFDYPPEL IIDHSCSNAI NCNNNNTNST NNNNINFDNW DQITHISIRE 850
IKKPFRVKVM GSTRIPLSCI KDIDSSSVIV SISLYHGIEC FSKAFTQPII 900
PPPFAFLAET LSVDWCEWLV FTNIDYSNLP VDARLSISVY SANETVDDVE 950
EIKNLDEATK KLTPIGWINV MITDFKYQLR QGMVELSLWP SDFSNPLGTC 1000
SNNPSSSQSV GLTLEFEEFN LPVLFPRKTK FSTSVSVIEQ PPTNINSNEM 1050
REFFEQITAL DPLSDLKQEK YNQLWTLRHY SILFPQVLPR LMLSVPWTQA 1100
TAVDEAISLL DRWPKLKPYE SLELLDAKHA NRKVREFAVT CLEDLSEDEL 1150
LDILLQLVQV LKYEPFHDSK LSRFLLRKAI LNRNIGHSFF WYLKSDLHDS 1200
NLSERFGILL ESYLYACGAH RIELLKQMEV INNLTEVAKK IKPLKDQDRR 1250
EFMIKEFESL EWPKRFHLTL NPRFESNGLI INKSKYMDSK KLPLRLSFTN 1300
TDMNADPIEV IFKAGDDLRQ DMLTLQMIRL MDKLWQKEGL DLKLSPYGCI 1350
STGDMIGMIE VVLNSETTAK IQKSEGGGAA SAFKLDPLAN WILQHNKSDM 1400
EYQKAVDTFI LSCAGYCVAT YVLGIGDRHN DNLMVTKGGR LFHIDFGHFL 1450
GNYKKKFGFK RERAPFVFTP DFCYVMGGKE SFKFSQFVNY CCTAYNIVRK 1500
NAKLFMNLFA MMVSTGIPEL QSMEDLNYLK ESFSIELSDE KAREKFVALI 1550
HESLATKTTQ LNNFFHHLAH A 1571
Length:1,571
Mass (Da):178,472
Last modified:December 4, 2007 - v2
Checksum:i9550F58C0E822C9F
GO

Sequence cautioni

The sequence AAA85721.1 differs from that shown. Reason: Frameshift at positions 1380 and 1391.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti749 – 7491D → H in AAA85721. 1 Publication
Sequence conflicti1494 – 14941A → G in AAA85721. 1 Publication
Sequence conflicti1540 – 15401E → A in AAA85721. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23476 mRNA. Translation: AAA85721.1. Frameshift.
AAFI02000024 Genomic DNA. Translation: EAL67965.1.
PIRiT18272.
RefSeqiXP_641955.1. XM_636863.1.

Genome annotation databases

EnsemblProtistsiDDB0214949; DDB0214949; DDB_G0278727.
GeneIDi8621687.
KEGGiddi:DDB_G0278727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U23476 mRNA. Translation: AAA85721.1 . Frameshift.
AAFI02000024 Genomic DNA. Translation: EAL67965.1 .
PIRi T18272.
RefSeqi XP_641955.1. XM_636863.1.

3D structure databases

ProteinModelPortali P54673.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0214949.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0214949 ; DDB0214949 ; DDB_G0278727 .
GeneIDi 8621687.
KEGGi ddi:DDB_G0278727.

Organism-specific databases

dictyBasei DDB_G0278727. pikA.

Phylogenomic databases

eggNOGi COG5032.
KOi K00922.
OMAi DAKHANR.
PhylomeDBi P54673.

Enzyme and pathway databases

BRENDAi 2.7.1.137. 1939.

Family and domain databases

Gene3Di 1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProi IPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
PANTHERi PTHR10048. PTHR10048. 1 hit.
Pfami PF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view ]
SMARTi SM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development."
    Zhou K., Takegawa K., Emr S.D., Firtel R.A.
    Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPI3K1_DICDI
AccessioniPrimary (citable) accession number: P54673
Secondary accession number(s): Q54XS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3