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P54673 (PI3K1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 3-kinase 1

Short name=PI3-kinase
Short name=PI3K
Short name=PtdIns-3-kinase
EC=2.7.1.137
Gene names
Name:pikA
Synonyms:pik1
ORF Names:DDB_G0278727
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1571 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 C2 PI3K-type domain.

Contains 1 PI3K-ABD domain.

Contains 1 PI3K-RBD domain.

Contains 1 PI3K/PI4K domain.

Contains 1 PIK helical domain.

Sequence caution

The sequence AAA85721.1 differs from that shown. Reason: Frameshift at positions 1380 and 1391.

Ontologies

Keywords
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 16267269. Source: dictyBase

aggregation involved in sorocarp development

Traceable author statement PubMed 15473840. Source: dictyBase

cell proliferation

Inferred from mutant phenotype PubMed 23843627. Source: dictyBase

cellular component movement

Inferred from genetic interaction PubMed 17462897. Source: dictyBase

chemotaxis

Inferred from mutant phenotype PubMed 16267269. Source: dictyBase

chemotaxis to cAMP

Inferred from genetic interaction PubMed 17535967. Source: dictyBase

macropinocytosis

Inferred from mutant phenotype PubMed 23843627. Source: dictyBase

negative chemotaxis

Inferred from genetic interaction PubMed 17517960. Source: dictyBase

phosphatidylinositol-3-phosphate biosynthetic process

Inferred from Biological aspect of Ancestor. Source: GOC

phosphatidylinositol-mediated signaling

Traceable author statement PubMed 15366706. Source: dictyBase

regulation of adenylate cyclase activity

Inferred from mutant phenotype PubMed 16267269. Source: dictyBase

regulation of cell morphogenesis

Inferred from genetic interaction PubMed 19011688. Source: dictyBase

regulation of ruffle assembly

Inferred from mutant phenotype PubMed 23843627. Source: dictyBase

response to bacterium

Inferred from mutant phenotype PubMed 21045112. Source: dictyBase

response to cAMP

Inferred from genetic interaction PubMed 16421252. Source: dictyBase

   Cellular_componentcell leading edge

Traceable author statement PubMed 15366706. Source: dictyBase

phosphatidylinositol 3-kinase complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

plasma membrane

Inferred from direct assay PubMed 23132928. Source: dictyBase

   Molecular_function1-phosphatidylinositol-3-kinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

1-phosphatidylinositol-4-phosphate 3-kinase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Ras GTPase binding

Inferred from physical interaction PubMed 10473630PubMed 23843627. Source: dictyBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15711571Phosphatidylinositol 3-kinase 1
PRO_0000088822

Regions

Domain530 – 62798PI3K-ABD
Domain700 – 78990PI3K-RBD
Domain851 – 1020170C2 PI3K-type
Domain1040 – 1216177PIK helical
Domain1309 – 1571263PI3K/PI4K
Compositional bias41 – 488Poly-Asn
Compositional bias59 – 7315Poly-Asn
Compositional bias161 – 1688Poly-Asn
Compositional bias294 – 30411Poly-Thr
Compositional bias308 – 32417Poly-Thr
Compositional bias413 – 42412Poly-Asn
Compositional bias503 – 5108Poly-Ser
Compositional bias570 – 57910Poly-Asn
Compositional bias821 – 8288Poly-Asn
Compositional bias831 – 8366Poly-Asn

Experimental info

Sequence conflict7491D → H in AAA85721. Ref.1
Sequence conflict14941A → G in AAA85721. Ref.1
Sequence conflict15401E → A in AAA85721. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54673 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 9550F58C0E822C9F

FASTA1,571178,472
        10         20         30         40         50         60 
MNSIESSSND SNEINKNSNK NNTHLNSNYN NIYKNNSTSS NNNNNHNNIE IIGIDNNKNN 

        70         80         90        100        110        120 
NKNNNDNNNN NNNIDKKRKD SKNKQNQEIN QEMSENKKIY NSNDSNCSSG SSSGGHVNNG 

       130        140        150        160        170        180 
HHILIEENER LEHENQEIQE IYKQKGMEFQ KKDLRFGYDV NSNNNNNNGG GSSSGSSSGG 

       190        200        210        220        230        240 
SDESASNQPI IRTRNREGSI LNLKKQGLVK EISQRFQTPD TASYTRPNAN NISIKDKISI 

       250        260        270        280        290        300 
LKKEQERRKQ DSEVQQREKV IVLSADSSNI QIYHPSVLIE KMNSKLDTEE KPATTTTTTT 

       310        320        330        340        350        360 
TTSTSISTST PTTTTTTTTN TSTTNDITIK PKTSPTKNNE ERSQSPITTP KQPVEEIVKK 

       370        380        390        400        410        420 
VSTPKSNNTS KKTSSDTTPT GKTTKKDKKD KKDKSRDSGN LVIVNNTNNT SSNNNNNNNN 

       430        440        450        460        470        480 
NNNNETIIKR RGRVLVTPSS DLKKNIQIYF TIPINPPVNK TNKPNQLLSN TSQQFLKTLI 

       490        500        510        520        530        540 
SNEIPIDCKI NDINDTDAFS DLSASASSSS FITKSSQSLL NVQSLRVKAI KTSFNILFLM 

       550        560        570        580        590        600 
PNQSKKILQV KGSDTIENLK ERIISDYLFN NNSNNNNNNC KYGADSYLIL DFNDNPMERS 

       610        620        630        640        650        660 
LVLNKSDYIL DKRAQGLIPK LKVIEKSTIL DSDPSDELSP SEYEIIRKLI PGTDTWRGEE 

       670        680        690        700        710        720 
VEYFRRVTSR LRYEALPLIK GSIQSTLLVR LSPLPIPIVG NKILISIFLP ITQVTKTLDL 

       730        740        750        760        770        780 
ELNETADQFT NRLFTKNYSK HLPNVNSNDF ILKVVGSSDF IHGPHDIRTF ESIRNHIIQG 

       790        800        810        820        830        840 
TKPQLTLIQR PKPELDPQPF KPRFDYPPEL IIDHSCSNAI NCNNNNTNST NNNNINFDNW 

       850        860        870        880        890        900 
DQITHISIRE IKKPFRVKVM GSTRIPLSCI KDIDSSSVIV SISLYHGIEC FSKAFTQPII 

       910        920        930        940        950        960 
PPPFAFLAET LSVDWCEWLV FTNIDYSNLP VDARLSISVY SANETVDDVE EIKNLDEATK 

       970        980        990       1000       1010       1020 
KLTPIGWINV MITDFKYQLR QGMVELSLWP SDFSNPLGTC SNNPSSSQSV GLTLEFEEFN 

      1030       1040       1050       1060       1070       1080 
LPVLFPRKTK FSTSVSVIEQ PPTNINSNEM REFFEQITAL DPLSDLKQEK YNQLWTLRHY 

      1090       1100       1110       1120       1130       1140 
SILFPQVLPR LMLSVPWTQA TAVDEAISLL DRWPKLKPYE SLELLDAKHA NRKVREFAVT 

      1150       1160       1170       1180       1190       1200 
CLEDLSEDEL LDILLQLVQV LKYEPFHDSK LSRFLLRKAI LNRNIGHSFF WYLKSDLHDS 

      1210       1220       1230       1240       1250       1260 
NLSERFGILL ESYLYACGAH RIELLKQMEV INNLTEVAKK IKPLKDQDRR EFMIKEFESL 

      1270       1280       1290       1300       1310       1320 
EWPKRFHLTL NPRFESNGLI INKSKYMDSK KLPLRLSFTN TDMNADPIEV IFKAGDDLRQ 

      1330       1340       1350       1360       1370       1380 
DMLTLQMIRL MDKLWQKEGL DLKLSPYGCI STGDMIGMIE VVLNSETTAK IQKSEGGGAA 

      1390       1400       1410       1420       1430       1440 
SAFKLDPLAN WILQHNKSDM EYQKAVDTFI LSCAGYCVAT YVLGIGDRHN DNLMVTKGGR 

      1450       1460       1470       1480       1490       1500 
LFHIDFGHFL GNYKKKFGFK RERAPFVFTP DFCYVMGGKE SFKFSQFVNY CCTAYNIVRK 

      1510       1520       1530       1540       1550       1560 
NAKLFMNLFA MMVSTGIPEL QSMEDLNYLK ESFSIELSDE KAREKFVALI HESLATKTTQ 

      1570 
LNNFFHHLAH A 

« Hide

References

« Hide 'large scale' references
[1]"A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development."
Zhou K., Takegawa K., Emr S.D., Firtel R.A.
Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AX3.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U23476 mRNA. Translation: AAA85721.1. Frameshift.
AAFI02000024 Genomic DNA. Translation: EAL67965.1.
PIRT18272.
RefSeqXP_641955.1. XM_636863.1.

3D structure databases

ProteinModelPortalP54673.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0214949.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0214949; DDB0214949; DDB_G0278727.
GeneID8621687.
KEGGddi:DDB_G0278727.

Organism-specific databases

dictyBaseDDB_G0278727. pikA.

Phylogenomic databases

eggNOGCOG5032.
OMADAKHANR.
PhylomeDBP54673.

Enzyme and pathway databases

BRENDA2.7.1.137. 1939.

Family and domain databases

Gene3D1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERPTHR10048. PTHR10048. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePI3K1_DICDI
AccessionPrimary (citable) accession number: P54673
Secondary accession number(s): Q54XS5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: July 9, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase