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P54673

- PI3K1_DICDI

UniProt

P54673 - PI3K1_DICDI

Protein

Phosphatidylinositol 3-kinase 1

Gene

pikA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-3-kinase activity Source: RefGenome
    2. 1-phosphatidylinositol-4-phosphate 3-kinase activity Source: RefGenome
    3. ATP binding Source: UniProtKB-KW
    4. Ras GTPase binding Source: dictyBase

    GO - Biological processi

    1. aggregation involved in sorocarp development Source: dictyBase
    2. cell proliferation Source: dictyBase
    3. cellular component movement Source: dictyBase
    4. chemotaxis Source: dictyBase
    5. chemotaxis to cAMP Source: dictyBase
    6. G-protein coupled receptor signaling pathway Source: dictyBase
    7. macropinocytosis Source: dictyBase
    8. negative chemotaxis Source: dictyBase
    9. phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
    10. phosphatidylinositol-mediated signaling Source: dictyBase
    11. regulation of adenylate cyclase activity Source: dictyBase
    12. regulation of cell morphogenesis Source: dictyBase
    13. regulation of ruffle assembly Source: dictyBase
    14. response to bacterium Source: dictyBase
    15. response to cAMP Source: dictyBase

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.1.137. 1939.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 3-kinase 1 (EC:2.7.1.137)
    Short name:
    PI3-kinase
    Short name:
    PI3K
    Short name:
    PtdIns-3-kinase
    Gene namesi
    Name:pikA
    Synonyms:pik1
    ORF Names:DDB_G0278727
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0278727. pikA.

    Subcellular locationi

    GO - Cellular componenti

    1. cell leading edge Source: dictyBase
    2. phosphatidylinositol 3-kinase complex Source: RefGenome
    3. plasma membrane Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15711571Phosphatidylinositol 3-kinase 1PRO_0000088822Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0214949.

    Structurei

    3D structure databases

    ProteinModelPortaliP54673.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini530 – 62798PI3K-ABDPROSITE-ProRule annotationAdd
    BLAST
    Domaini700 – 78990PI3K-RBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini851 – 1020170C2 PI3K-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini1040 – 1216177PIK helicalPROSITE-ProRule annotationAdd
    BLAST
    Domaini1309 – 1571263PI3K/PI4KPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi41 – 488Poly-Asn
    Compositional biasi59 – 7315Poly-AsnAdd
    BLAST
    Compositional biasi161 – 1688Poly-Asn
    Compositional biasi294 – 30411Poly-ThrAdd
    BLAST
    Compositional biasi308 – 32417Poly-ThrAdd
    BLAST
    Compositional biasi413 – 42412Poly-AsnAdd
    BLAST
    Compositional biasi503 – 5108Poly-Ser
    Compositional biasi570 – 57910Poly-Asn
    Compositional biasi821 – 8288Poly-Asn
    Compositional biasi831 – 8366Poly-Asn

    Sequence similaritiesi

    Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
    Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
    Contains 1 PI3K-ABD domain.PROSITE-ProRule annotation
    Contains 1 PI3K-RBD domain.PROSITE-ProRule annotation
    Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
    Contains 1 PIK helical domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5032.
    KOiK00922.
    OMAiDAKHANR.
    PhylomeDBiP54673.

    Family and domain databases

    Gene3Di1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR003113. PI3K_adapt-bd_dom.
    IPR002420. PI3K_C2_dom.
    IPR000341. PI3K_Ras-bd_dom.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR10048. PTHR10048. 1 hit.
    PfamiPF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00794. PI3K_rbd. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view]
    SMARTiSM00142. PI3K_C2. 1 hit.
    SM00144. PI3K_rbd. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51544. PI3K_ABD. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51546. PI3K_RBD. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P54673-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSIESSSND SNEINKNSNK NNTHLNSNYN NIYKNNSTSS NNNNNHNNIE     50
    IIGIDNNKNN NKNNNDNNNN NNNIDKKRKD SKNKQNQEIN QEMSENKKIY 100
    NSNDSNCSSG SSSGGHVNNG HHILIEENER LEHENQEIQE IYKQKGMEFQ 150
    KKDLRFGYDV NSNNNNNNGG GSSSGSSSGG SDESASNQPI IRTRNREGSI 200
    LNLKKQGLVK EISQRFQTPD TASYTRPNAN NISIKDKISI LKKEQERRKQ 250
    DSEVQQREKV IVLSADSSNI QIYHPSVLIE KMNSKLDTEE KPATTTTTTT 300
    TTSTSISTST PTTTTTTTTN TSTTNDITIK PKTSPTKNNE ERSQSPITTP 350
    KQPVEEIVKK VSTPKSNNTS KKTSSDTTPT GKTTKKDKKD KKDKSRDSGN 400
    LVIVNNTNNT SSNNNNNNNN NNNNETIIKR RGRVLVTPSS DLKKNIQIYF 450
    TIPINPPVNK TNKPNQLLSN TSQQFLKTLI SNEIPIDCKI NDINDTDAFS 500
    DLSASASSSS FITKSSQSLL NVQSLRVKAI KTSFNILFLM PNQSKKILQV 550
    KGSDTIENLK ERIISDYLFN NNSNNNNNNC KYGADSYLIL DFNDNPMERS 600
    LVLNKSDYIL DKRAQGLIPK LKVIEKSTIL DSDPSDELSP SEYEIIRKLI 650
    PGTDTWRGEE VEYFRRVTSR LRYEALPLIK GSIQSTLLVR LSPLPIPIVG 700
    NKILISIFLP ITQVTKTLDL ELNETADQFT NRLFTKNYSK HLPNVNSNDF 750
    ILKVVGSSDF IHGPHDIRTF ESIRNHIIQG TKPQLTLIQR PKPELDPQPF 800
    KPRFDYPPEL IIDHSCSNAI NCNNNNTNST NNNNINFDNW DQITHISIRE 850
    IKKPFRVKVM GSTRIPLSCI KDIDSSSVIV SISLYHGIEC FSKAFTQPII 900
    PPPFAFLAET LSVDWCEWLV FTNIDYSNLP VDARLSISVY SANETVDDVE 950
    EIKNLDEATK KLTPIGWINV MITDFKYQLR QGMVELSLWP SDFSNPLGTC 1000
    SNNPSSSQSV GLTLEFEEFN LPVLFPRKTK FSTSVSVIEQ PPTNINSNEM 1050
    REFFEQITAL DPLSDLKQEK YNQLWTLRHY SILFPQVLPR LMLSVPWTQA 1100
    TAVDEAISLL DRWPKLKPYE SLELLDAKHA NRKVREFAVT CLEDLSEDEL 1150
    LDILLQLVQV LKYEPFHDSK LSRFLLRKAI LNRNIGHSFF WYLKSDLHDS 1200
    NLSERFGILL ESYLYACGAH RIELLKQMEV INNLTEVAKK IKPLKDQDRR 1250
    EFMIKEFESL EWPKRFHLTL NPRFESNGLI INKSKYMDSK KLPLRLSFTN 1300
    TDMNADPIEV IFKAGDDLRQ DMLTLQMIRL MDKLWQKEGL DLKLSPYGCI 1350
    STGDMIGMIE VVLNSETTAK IQKSEGGGAA SAFKLDPLAN WILQHNKSDM 1400
    EYQKAVDTFI LSCAGYCVAT YVLGIGDRHN DNLMVTKGGR LFHIDFGHFL 1450
    GNYKKKFGFK RERAPFVFTP DFCYVMGGKE SFKFSQFVNY CCTAYNIVRK 1500
    NAKLFMNLFA MMVSTGIPEL QSMEDLNYLK ESFSIELSDE KAREKFVALI 1550
    HESLATKTTQ LNNFFHHLAH A 1571
    Length:1,571
    Mass (Da):178,472
    Last modified:December 4, 2007 - v2
    Checksum:i9550F58C0E822C9F
    GO

    Sequence cautioni

    The sequence AAA85721.1 differs from that shown. Reason: Frameshift at positions 1380 and 1391.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti749 – 7491D → H in AAA85721. (PubMed:7565716)Curated
    Sequence conflicti1494 – 14941A → G in AAA85721. (PubMed:7565716)Curated
    Sequence conflicti1540 – 15401E → A in AAA85721. (PubMed:7565716)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23476 mRNA. Translation: AAA85721.1. Frameshift.
    AAFI02000024 Genomic DNA. Translation: EAL67965.1.
    PIRiT18272.
    RefSeqiXP_641955.1. XM_636863.1.

    Genome annotation databases

    EnsemblProtistsiDDB0214949; DDB0214949; DDB_G0278727.
    GeneIDi8621687.
    KEGGiddi:DDB_G0278727.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U23476 mRNA. Translation: AAA85721.1 . Frameshift.
    AAFI02000024 Genomic DNA. Translation: EAL67965.1 .
    PIRi T18272.
    RefSeqi XP_641955.1. XM_636863.1.

    3D structure databases

    ProteinModelPortali P54673.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0214949.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0214949 ; DDB0214949 ; DDB_G0278727 .
    GeneIDi 8621687.
    KEGGi ddi:DDB_G0278727.

    Organism-specific databases

    dictyBasei DDB_G0278727. pikA.

    Phylogenomic databases

    eggNOGi COG5032.
    KOi K00922.
    OMAi DAKHANR.
    PhylomeDBi P54673.

    Enzyme and pathway databases

    BRENDAi 2.7.1.137. 1939.

    Family and domain databases

    Gene3Di 1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR003113. PI3K_adapt-bd_dom.
    IPR002420. PI3K_C2_dom.
    IPR000341. PI3K_Ras-bd_dom.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR10048. PTHR10048. 1 hit.
    Pfami PF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00794. PI3K_rbd. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view ]
    SMARTi SM00142. PI3K_C2. 1 hit.
    SM00144. PI3K_rbd. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51544. PI3K_ABD. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51546. PI3K_RBD. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development."
      Zhou K., Takegawa K., Emr S.D., Firtel R.A.
      Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: AX3.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiPI3K1_DICDI
    AccessioniPrimary (citable) accession number: P54673
    Secondary accession number(s): Q54XS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3