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Protein

Phosphatidylinositol 3-kinase 1

Gene

pikA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: dictyBase
  • aggregation involved in sorocarp development Source: dictyBase
  • asexual reproduction Source: dictyBase
  • cell migration Source: GO_Central
  • cell motility Source: dictyBase
  • chemotaxis Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • G-protein coupled receptor signaling pathway Source: dictyBase
  • macropinocytosis Source: dictyBase
  • negative chemotaxis Source: dictyBase
  • phosphatidylinositol 3-kinase signaling Source: GO_Central
  • phosphatidylinositol-3-phosphate biosynthetic process Source: GO_Central
  • phosphatidylinositol biosynthetic process Source: dictyBase
  • phosphatidylinositol-mediated signaling Source: dictyBase
  • phosphatidylinositol phosphorylation Source: GO_Central
  • regulation of adenylate cyclase activity Source: dictyBase
  • regulation of cell morphogenesis Source: dictyBase
  • regulation of chemotaxis Source: dictyBase
  • regulation of ruffle assembly Source: dictyBase
  • response to bacterium Source: dictyBase
  • response to cAMP Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase 1 (EC:2.7.1.137)
Short name:
PI3-kinase
Short name:
PI3K
Short name:
PtdIns-3-kinase
Gene namesi
Name:pikA
Synonyms:pik1
ORF Names:DDB_G0278727
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278727. pikA.

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: dictyBase
  • cytoplasm Source: GO_Central
  • phosphatidylinositol 3-kinase complex Source: GO_Central
  • plasma membrane Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15711571Phosphatidylinositol 3-kinase 1PRO_0000088822Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0214949.

Structurei

3D structure databases

ProteinModelPortaliP54673.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini530 – 62798PI3K-ABDPROSITE-ProRule annotationAdd
BLAST
Domaini700 – 78990PI3K-RBDPROSITE-ProRule annotationAdd
BLAST
Domaini851 – 1020170C2 PI3K-typePROSITE-ProRule annotationAdd
BLAST
Domaini1040 – 1216177PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini1309 – 1571263PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 488Poly-Asn
Compositional biasi59 – 7315Poly-AsnAdd
BLAST
Compositional biasi161 – 1688Poly-Asn
Compositional biasi294 – 30411Poly-ThrAdd
BLAST
Compositional biasi308 – 32417Poly-ThrAdd
BLAST
Compositional biasi413 – 42412Poly-AsnAdd
BLAST
Compositional biasi503 – 5108Poly-Ser
Compositional biasi570 – 57910Poly-Asn
Compositional biasi821 – 8288Poly-Asn
Compositional biasi831 – 8366Poly-Asn

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K-ABD domain.PROSITE-ProRule annotation
Contains 1 PI3K-RBD domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5032.
InParanoidiP54673.
KOiK00922.
OMAiDAKHANR.
PhylomeDBiP54673.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P54673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSIESSSND SNEINKNSNK NNTHLNSNYN NIYKNNSTSS NNNNNHNNIE
60 70 80 90 100
IIGIDNNKNN NKNNNDNNNN NNNIDKKRKD SKNKQNQEIN QEMSENKKIY
110 120 130 140 150
NSNDSNCSSG SSSGGHVNNG HHILIEENER LEHENQEIQE IYKQKGMEFQ
160 170 180 190 200
KKDLRFGYDV NSNNNNNNGG GSSSGSSSGG SDESASNQPI IRTRNREGSI
210 220 230 240 250
LNLKKQGLVK EISQRFQTPD TASYTRPNAN NISIKDKISI LKKEQERRKQ
260 270 280 290 300
DSEVQQREKV IVLSADSSNI QIYHPSVLIE KMNSKLDTEE KPATTTTTTT
310 320 330 340 350
TTSTSISTST PTTTTTTTTN TSTTNDITIK PKTSPTKNNE ERSQSPITTP
360 370 380 390 400
KQPVEEIVKK VSTPKSNNTS KKTSSDTTPT GKTTKKDKKD KKDKSRDSGN
410 420 430 440 450
LVIVNNTNNT SSNNNNNNNN NNNNETIIKR RGRVLVTPSS DLKKNIQIYF
460 470 480 490 500
TIPINPPVNK TNKPNQLLSN TSQQFLKTLI SNEIPIDCKI NDINDTDAFS
510 520 530 540 550
DLSASASSSS FITKSSQSLL NVQSLRVKAI KTSFNILFLM PNQSKKILQV
560 570 580 590 600
KGSDTIENLK ERIISDYLFN NNSNNNNNNC KYGADSYLIL DFNDNPMERS
610 620 630 640 650
LVLNKSDYIL DKRAQGLIPK LKVIEKSTIL DSDPSDELSP SEYEIIRKLI
660 670 680 690 700
PGTDTWRGEE VEYFRRVTSR LRYEALPLIK GSIQSTLLVR LSPLPIPIVG
710 720 730 740 750
NKILISIFLP ITQVTKTLDL ELNETADQFT NRLFTKNYSK HLPNVNSNDF
760 770 780 790 800
ILKVVGSSDF IHGPHDIRTF ESIRNHIIQG TKPQLTLIQR PKPELDPQPF
810 820 830 840 850
KPRFDYPPEL IIDHSCSNAI NCNNNNTNST NNNNINFDNW DQITHISIRE
860 870 880 890 900
IKKPFRVKVM GSTRIPLSCI KDIDSSSVIV SISLYHGIEC FSKAFTQPII
910 920 930 940 950
PPPFAFLAET LSVDWCEWLV FTNIDYSNLP VDARLSISVY SANETVDDVE
960 970 980 990 1000
EIKNLDEATK KLTPIGWINV MITDFKYQLR QGMVELSLWP SDFSNPLGTC
1010 1020 1030 1040 1050
SNNPSSSQSV GLTLEFEEFN LPVLFPRKTK FSTSVSVIEQ PPTNINSNEM
1060 1070 1080 1090 1100
REFFEQITAL DPLSDLKQEK YNQLWTLRHY SILFPQVLPR LMLSVPWTQA
1110 1120 1130 1140 1150
TAVDEAISLL DRWPKLKPYE SLELLDAKHA NRKVREFAVT CLEDLSEDEL
1160 1170 1180 1190 1200
LDILLQLVQV LKYEPFHDSK LSRFLLRKAI LNRNIGHSFF WYLKSDLHDS
1210 1220 1230 1240 1250
NLSERFGILL ESYLYACGAH RIELLKQMEV INNLTEVAKK IKPLKDQDRR
1260 1270 1280 1290 1300
EFMIKEFESL EWPKRFHLTL NPRFESNGLI INKSKYMDSK KLPLRLSFTN
1310 1320 1330 1340 1350
TDMNADPIEV IFKAGDDLRQ DMLTLQMIRL MDKLWQKEGL DLKLSPYGCI
1360 1370 1380 1390 1400
STGDMIGMIE VVLNSETTAK IQKSEGGGAA SAFKLDPLAN WILQHNKSDM
1410 1420 1430 1440 1450
EYQKAVDTFI LSCAGYCVAT YVLGIGDRHN DNLMVTKGGR LFHIDFGHFL
1460 1470 1480 1490 1500
GNYKKKFGFK RERAPFVFTP DFCYVMGGKE SFKFSQFVNY CCTAYNIVRK
1510 1520 1530 1540 1550
NAKLFMNLFA MMVSTGIPEL QSMEDLNYLK ESFSIELSDE KAREKFVALI
1560 1570
HESLATKTTQ LNNFFHHLAH A
Length:1,571
Mass (Da):178,472
Last modified:December 4, 2007 - v2
Checksum:i9550F58C0E822C9F
GO

Sequence cautioni

The sequence AAA85721.1 differs from that shown. Reason: Frameshift at positions 1380 and 1391. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti749 – 7491D → H in AAA85721 (PubMed:7565716).Curated
Sequence conflicti1494 – 14941A → G in AAA85721 (PubMed:7565716).Curated
Sequence conflicti1540 – 15401E → A in AAA85721 (PubMed:7565716).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23476 mRNA. Translation: AAA85721.1. Frameshift.
AAFI02000024 Genomic DNA. Translation: EAL67965.1.
PIRiT18272.
RefSeqiXP_641955.1. XM_636863.1.

Genome annotation databases

EnsemblProtistsiDDB0214949; DDB0214949; DDB_G0278727.
GeneIDi8621687.
KEGGiddi:DDB_G0278727.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23476 mRNA. Translation: AAA85721.1. Frameshift.
AAFI02000024 Genomic DNA. Translation: EAL67965.1.
PIRiT18272.
RefSeqiXP_641955.1. XM_636863.1.

3D structure databases

ProteinModelPortaliP54673.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0214949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0214949; DDB0214949; DDB_G0278727.
GeneIDi8621687.
KEGGiddi:DDB_G0278727.

Organism-specific databases

dictyBaseiDDB_G0278727. pikA.

Phylogenomic databases

eggNOGiCOG5032.
InParanoidiP54673.
KOiK00922.
OMAiDAKHANR.
PhylomeDBiP54673.

Enzyme and pathway databases

BRENDAi2.7.1.137. 1939.

Miscellaneous databases

PROiP54673.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003113. PI3K_adapt-bd_dom.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
SMARTiSM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51544. PI3K_ABD. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development."
    Zhou K., Takegawa K., Emr S.D., Firtel R.A.
    Mol. Cell. Biol. 15:5645-5656(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX3.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiPI3K1_DICDI
AccessioniPrimary (citable) accession number: P54673
Secondary accession number(s): Q54XS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: June 24, 2015
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.