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Protein

Phosphatidylinositol 3-kinase 1

Gene

pikA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: dictyBase
  • activation of protein kinase activity Source: dictyBase
  • aggregation involved in sorocarp development Source: dictyBase
  • asexual reproduction Source: dictyBase
  • cell migration Source: GO_Central
  • cell motility Source: dictyBase
  • chemotaxis Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • G-protein coupled receptor signaling pathway Source: dictyBase
  • macropinocytosis Source: dictyBase
  • maintenance of cell polarity Source: dictyBase
  • negative chemotaxis Source: dictyBase
  • phagolysosome assembly Source: dictyBase
  • phagosome maturation Source: dictyBase
  • phosphatidylinositol 3-kinase signaling Source: dictyBase
  • phosphatidylinositol biosynthetic process Source: dictyBase
  • phosphatidylinositol-mediated signaling Source: dictyBase
  • positive regulation of macropinocytosis Source: dictyBase
  • pseudopodium assembly Source: dictyBase
  • regulation of adenylate cyclase activity Source: dictyBase
  • regulation of cell morphogenesis Source: dictyBase
  • regulation of chemotaxis Source: dictyBase
  • regulation of ruffle assembly Source: dictyBase
  • response to bacterium Source: dictyBase
  • response to cAMP Source: dictyBase

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.137 1939

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase 1 (EC:2.7.1.137)
Short name:
PI3-kinase
Short name:
PI3K
Short name:
PtdIns-3-kinase
Gene namesi
Name:pikA
Synonyms:pik1
ORF Names:DDB_G0278727
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278727 pikA

Subcellular locationi

GO - Cellular componenti

  • cell leading edge Source: dictyBase
  • intracellular Source: GOC
  • phosphatidylinositol 3-kinase complex Source: GO_Central
  • plasma membrane Source: dictyBase
  • pseudopodium Source: dictyBase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000888221 – 1571Phosphatidylinositol 3-kinase 1Add BLAST1571

Proteomic databases

PaxDbiP54673
PRIDEiP54673

Interactioni

GO - Molecular functioni

  • Ras GTPase binding Source: dictyBase

Protein-protein interaction databases

STRINGi44689.DDB0214949

Structurei

3D structure databases

ProteinModelPortaliP54673
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini530 – 627PI3K-ABDPROSITE-ProRule annotationAdd BLAST98
Domaini700 – 789PI3K-RBDPROSITE-ProRule annotationAdd BLAST90
Domaini851 – 1020C2 PI3K-typePROSITE-ProRule annotationAdd BLAST170
Domaini1040 – 1216PIK helicalPROSITE-ProRule annotationAdd BLAST177
Domaini1309 – 1571PI3K/PI4KPROSITE-ProRule annotationAdd BLAST263

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 48Poly-Asn8
Compositional biasi59 – 73Poly-AsnAdd BLAST15
Compositional biasi161 – 168Poly-Asn8
Compositional biasi294 – 304Poly-ThrAdd BLAST11
Compositional biasi308 – 324Poly-ThrAdd BLAST17
Compositional biasi413 – 424Poly-AsnAdd BLAST12
Compositional biasi503 – 510Poly-Ser8
Compositional biasi570 – 579Poly-Asn10
Compositional biasi821 – 828Poly-Asn8
Compositional biasi831 – 836Poly-Asn6

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0904 Eukaryota
COG5032 LUCA
InParanoidiP54673
KOiK00922
OMAiMIGMIEV
PhylomeDBiP54673

Family and domain databases

CDDicd00891 PI3Kc, 1 hit
Gene3Di1.10.1070.11, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR035892 C2_domain_sf
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR003113 PI3K_adapt-bd_dom
IPR002420 PI3K_C2_dom
IPR000341 PI3K_Ras-bd_dom
IPR035448 PI3Kc
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR10048 PTHR10048, 2 hits
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
PF00792 PI3K_C2, 1 hit
PF00794 PI3K_rbd, 1 hit
PF00613 PI3Ka, 1 hit
SMARTiView protein in SMART
SM00142 PI3K_C2, 1 hit
SM00144 PI3K_rbd, 1 hit
SM00145 PI3Ka, 1 hit
SM00146 PI3Kc, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51544 PI3K_ABD, 1 hit
PS51547 PI3K_C2, 1 hit
PS51546 PI3K_RBD, 1 hit
PS51545 PIK_HELICAL, 1 hit

Sequencei

Sequence statusi: Complete.

P54673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSIESSSND SNEINKNSNK NNTHLNSNYN NIYKNNSTSS NNNNNHNNIE
60 70 80 90 100
IIGIDNNKNN NKNNNDNNNN NNNIDKKRKD SKNKQNQEIN QEMSENKKIY
110 120 130 140 150
NSNDSNCSSG SSSGGHVNNG HHILIEENER LEHENQEIQE IYKQKGMEFQ
160 170 180 190 200
KKDLRFGYDV NSNNNNNNGG GSSSGSSSGG SDESASNQPI IRTRNREGSI
210 220 230 240 250
LNLKKQGLVK EISQRFQTPD TASYTRPNAN NISIKDKISI LKKEQERRKQ
260 270 280 290 300
DSEVQQREKV IVLSADSSNI QIYHPSVLIE KMNSKLDTEE KPATTTTTTT
310 320 330 340 350
TTSTSISTST PTTTTTTTTN TSTTNDITIK PKTSPTKNNE ERSQSPITTP
360 370 380 390 400
KQPVEEIVKK VSTPKSNNTS KKTSSDTTPT GKTTKKDKKD KKDKSRDSGN
410 420 430 440 450
LVIVNNTNNT SSNNNNNNNN NNNNETIIKR RGRVLVTPSS DLKKNIQIYF
460 470 480 490 500
TIPINPPVNK TNKPNQLLSN TSQQFLKTLI SNEIPIDCKI NDINDTDAFS
510 520 530 540 550
DLSASASSSS FITKSSQSLL NVQSLRVKAI KTSFNILFLM PNQSKKILQV
560 570 580 590 600
KGSDTIENLK ERIISDYLFN NNSNNNNNNC KYGADSYLIL DFNDNPMERS
610 620 630 640 650
LVLNKSDYIL DKRAQGLIPK LKVIEKSTIL DSDPSDELSP SEYEIIRKLI
660 670 680 690 700
PGTDTWRGEE VEYFRRVTSR LRYEALPLIK GSIQSTLLVR LSPLPIPIVG
710 720 730 740 750
NKILISIFLP ITQVTKTLDL ELNETADQFT NRLFTKNYSK HLPNVNSNDF
760 770 780 790 800
ILKVVGSSDF IHGPHDIRTF ESIRNHIIQG TKPQLTLIQR PKPELDPQPF
810 820 830 840 850
KPRFDYPPEL IIDHSCSNAI NCNNNNTNST NNNNINFDNW DQITHISIRE
860 870 880 890 900
IKKPFRVKVM GSTRIPLSCI KDIDSSSVIV SISLYHGIEC FSKAFTQPII
910 920 930 940 950
PPPFAFLAET LSVDWCEWLV FTNIDYSNLP VDARLSISVY SANETVDDVE
960 970 980 990 1000
EIKNLDEATK KLTPIGWINV MITDFKYQLR QGMVELSLWP SDFSNPLGTC
1010 1020 1030 1040 1050
SNNPSSSQSV GLTLEFEEFN LPVLFPRKTK FSTSVSVIEQ PPTNINSNEM
1060 1070 1080 1090 1100
REFFEQITAL DPLSDLKQEK YNQLWTLRHY SILFPQVLPR LMLSVPWTQA
1110 1120 1130 1140 1150
TAVDEAISLL DRWPKLKPYE SLELLDAKHA NRKVREFAVT CLEDLSEDEL
1160 1170 1180 1190 1200
LDILLQLVQV LKYEPFHDSK LSRFLLRKAI LNRNIGHSFF WYLKSDLHDS
1210 1220 1230 1240 1250
NLSERFGILL ESYLYACGAH RIELLKQMEV INNLTEVAKK IKPLKDQDRR
1260 1270 1280 1290 1300
EFMIKEFESL EWPKRFHLTL NPRFESNGLI INKSKYMDSK KLPLRLSFTN
1310 1320 1330 1340 1350
TDMNADPIEV IFKAGDDLRQ DMLTLQMIRL MDKLWQKEGL DLKLSPYGCI
1360 1370 1380 1390 1400
STGDMIGMIE VVLNSETTAK IQKSEGGGAA SAFKLDPLAN WILQHNKSDM
1410 1420 1430 1440 1450
EYQKAVDTFI LSCAGYCVAT YVLGIGDRHN DNLMVTKGGR LFHIDFGHFL
1460 1470 1480 1490 1500
GNYKKKFGFK RERAPFVFTP DFCYVMGGKE SFKFSQFVNY CCTAYNIVRK
1510 1520 1530 1540 1550
NAKLFMNLFA MMVSTGIPEL QSMEDLNYLK ESFSIELSDE KAREKFVALI
1560 1570
HESLATKTTQ LNNFFHHLAH A
Length:1,571
Mass (Da):178,472
Last modified:December 4, 2007 - v2
Checksum:i9550F58C0E822C9F
GO

Sequence cautioni

The sequence AAA85721 differs from that shown. Reason: Frameshift at positions 1380 and 1391.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti749D → H in AAA85721 (PubMed:7565716).Curated1
Sequence conflicti1494A → G in AAA85721 (PubMed:7565716).Curated1
Sequence conflicti1540E → A in AAA85721 (PubMed:7565716).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23476 mRNA Translation: AAA85721.1 Frameshift.
AAFI02000024 Genomic DNA Translation: EAL67965.1
PIRiT18272
RefSeqiXP_641955.1, XM_636863.1

Genome annotation databases

EnsemblProtistsiEAL67965; EAL67965; DDB_G0278727
GeneIDi8621687
KEGGiddi:DDB_G0278727

Entry informationi

Entry nameiPI3K1_DICDI
AccessioniPrimary (citable) accession number: P54673
Secondary accession number(s): Q54XS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health