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Protein

Acetylornithine deacetylase

Gene

argE

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

N(2)-acetyl-L-ornithine + H2O = acetate + L-ornithine.

Cofactori

Zn2+By similarity, Co2+By similarityNote: Binds 2 Zn2+ or Co2+ ions per subunit.By similarity

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine from N(2)-acetyl-L-ornithine (linear).
Proteins known to be involved in this subpathway in this organism are:
  1. Acetylornithine deacetylase (argE)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine from N(2)-acetyl-L-ornithine (linear), the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Cobalt or zinc 1By similarity
Active sitei105 – 1051By similarity
Metal bindingi135 – 1351Cobalt or zinc 1By similarity
Metal bindingi135 – 1351Cobalt or zinc 2By similarity
Active sitei169 – 1691Proton acceptorBy similarity
Metal bindingi170 – 1701Cobalt or zinc 2By similarity
Metal bindingi419 – 4191Cobalt or zinc 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

Cobalt, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00068; UER00110.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylornithine deacetylase (EC:3.5.1.16)
Alternative name(s):
N-acetylornithinase
Short name:
AO
Short name:
Acetylornithinase
Short name:
NAO
Gene namesi
Name:argE
Synonyms:aodD
ORF Names:DDB_G0267380
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0267380. argE.

Subcellular locationi

GO - Cellular componenti

  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Acetylornithine deacetylasePRO_0000185256Add
BLAST

Proteomic databases

PaxDbiP54638.
PRIDEiP54638.

2D gel databases

SWISS-2DPAGEP54638.

Expressioni

Developmental stagei

Expressed during development. Expression levels decrease steadily from the initiation of development until culmination. Levels increase after 18 hours of development and peak at 22 hours, after which they decrease again.2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191165.

Structurei

3D structure databases

ProteinModelPortaliP54638.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family. ArgE subfamily.Curated

Phylogenomic databases

eggNOGiKOG2276. Eukaryota.
COG0624. LUCA.
InParanoidiP54638.
KOiK01438.
OMAiITWHLKA.
PhylomeDBiP54638.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
SUPFAMiSSF55031. SSF55031. 1 hit.

Sequencei

Sequence statusi: Complete.

P54638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKPVASYEL DEKRFLTLLG KLIGETENLQ NRPPALIPIE DNAGRHVIEA
60 70 80 90 100
LTPYLKANGG VLELEQVHCD PVNYPKRGNI IIEYPGTSKG TSSPKTISFV
110 120 130 140 150
GSHLDVVPAD KTAWDRNPFQ LIIEGDKLYG RGTTDCLGHV ALLTDLFIQL
160 170 180 190 200
ATEKPALKHS IFAVFIVSEE NDEIPGIGVD ALDHSGKMNP CKNGPVYWVD
210 220 230 240 250
SADSQPTIGT GGAQTWNLTA HGKNMHSAMP YRTVNSVELV NEALAEIQRR
260 270 280 290 300
FYIDFKPHPK EAEYKFDCSS TMKPTLWKPI AGSYNTIPGE STICGDIRLT
310 320 330 340 350
PFYDMKEMRA KVEGYIKDIN ANITELRNRG PYSKYDVPAS EGVEPVKGSV
360 370 380 390 400
SIEWLGEASA GVACKLDSDG YKALGKATSE ILGSLTPVAT CGTLPLVRDL
410 420 430 440
QDSGFDIQIT GFGKEETYHA DNEYALLSDF KNAIKILSRT IDLLEKN
Length:447
Mass (Da):49,070
Last modified:May 29, 2007 - v2
Checksum:i115B0A123C1A3A7B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531P → S in AAB04942 (Ref. 1) Curated
Sequence conflicti63 – 631E → A in AAB04942 (Ref. 1) Curated
Sequence conflicti212 – 2121G → GG in AAB04942 (Ref. 1) Curated
Sequence conflicti254 – 2541D → N in AAB04942 (Ref. 1) Curated
Sequence conflicti398 – 3981R → L in AAB04942 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23957 mRNA. Translation: AAB04942.1.
AAFI02000003 Genomic DNA. Translation: EAL73142.1.
RefSeqiXP_647163.1. XM_642071.1.

Genome annotation databases

EnsemblProtistsiDDB0191165; DDB0191165; DDB_G0267380.
GeneIDi8615966.
KEGGiddi:DDB_G0267380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23957 mRNA. Translation: AAB04942.1.
AAFI02000003 Genomic DNA. Translation: EAL73142.1.
RefSeqiXP_647163.1. XM_642071.1.

3D structure databases

ProteinModelPortaliP54638.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191165.

2D gel databases

SWISS-2DPAGEP54638.

Proteomic databases

PaxDbiP54638.
PRIDEiP54638.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191165; DDB0191165; DDB_G0267380.
GeneIDi8615966.
KEGGiddi:DDB_G0267380.

Organism-specific databases

dictyBaseiDDB_G0267380. argE.

Phylogenomic databases

eggNOGiKOG2276. Eukaryota.
COG0624. LUCA.
InParanoidiP54638.
KOiK01438.
OMAiITWHLKA.
PhylomeDBiP54638.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00110.

Miscellaneous databases

PROiP54638.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
InterProiIPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
SUPFAMiSSF55031. SSF55031. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARGE_DICDI
AccessioniPrimary (citable) accession number: P54638
Secondary accession number(s): Q55GM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 29, 2007
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.