Reviewed, UniProtKB/Swiss-Prot P54638 (ARGE_DICDI)

Names and origin

Protein names

Recommended name:
    Acetylornithine deacetylase
    EC=3.5.1.16
Alternative name(s):
    N-acetylornithinase
      Short name=NAO
      Short name=Acetylornithinase
      Short name=AO

Gene names

Name:	argE
Synonyms:	aodD
ORF Names:	DDB_G0267380

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + H2O = acetate + L-ornithine.
Cofactor	Binds 2 zinc or cobalt ions per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine from N(2)-acetyl-L-ornithine (linear): step 1/1.
Subunit structure	Homodimer By similarity.
Developmental stage	Expressed during development. Expression levels decrease steadily from the initiation of development until culmination. Levels increase after 18 hours of development and peak at 22 hours, after which they decrease again. Ref.3 Ref.4
Sequence similarities	Belongs to the peptidase M20A family. ArgE subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Ligand	Cobalt Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	acetylornithine deacetylase activity Inferred from electronic annotation. Source: EC cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Acetylornithine deacetylase		PRO_0000185256
Sites
	Active site	105	1	By similarity
	Active site	169	1	Proton acceptor By similarity
	Metal binding	103	1	Cobalt or zinc 1 By similarity
	Metal binding	135	1	Cobalt or zinc 1 By similarity
	Metal binding	135	1	Cobalt or zinc 2 By similarity
	Metal binding	170	1	Cobalt or zinc 2 By similarity
	Metal binding	419	1	Cobalt or zinc 2 By similarity
Experimental info
	Sequence conflict	53	1	P → S in AAB04942. Ref.1
	Sequence conflict	63	1	E → A in AAB04942. Ref.1
	Sequence conflict	212	1	G → GG in AAB04942. Ref.1
	Sequence conflict	254	1	D → N in AAB04942. Ref.1
	Sequence conflict	398	1	R → L in AAB04942. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54638-1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 115B0A123C1A3A7B	FASTA	447	49,070
	10 20 30 40 50 60 MTKPVASYEL DEKRFLTLLG KLIGETENLQ NRPPALIPIE DNAGRHVIEA LTPYLKANGG 70 80 90 100 110 120 VLELEQVHCD PVNYPKRGNI IIEYPGTSKG TSSPKTISFV GSHLDVVPAD KTAWDRNPFQ 130 140 150 160 170 180 LIIEGDKLYG RGTTDCLGHV ALLTDLFIQL ATEKPALKHS IFAVFIVSEE NDEIPGIGVD 190 200 210 220 230 240 ALDHSGKMNP CKNGPVYWVD SADSQPTIGT GGAQTWNLTA HGKNMHSAMP YRTVNSVELV 250 260 270 280 290 300 NEALAEIQRR FYIDFKPHPK EAEYKFDCSS TMKPTLWKPI AGSYNTIPGE STICGDIRLT 310 320 330 340 350 360 PFYDMKEMRA KVEGYIKDIN ANITELRNRG PYSKYDVPAS EGVEPVKGSV SIEWLGEASA 370 380 390 400 410 420 GVACKLDSDG YKALGKATSE ILGSLTPVAT CGTLPLVRDL QDSGFDIQIT GFGKEETYHA 430 440 DNEYALLSDF KNAIKILSRT IDLLEKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence analysis of a member of the argE/dapE/Acy1/CPG2/yscS protein family in Dictyostelium discoideum." Lu C., Mann K., Marriott G. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"A transcriptional profile of multicellular development in Dictyostelium discoideum." Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M., Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H., Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G. Development 129:1543-1552(2002) [PubMed: 11923193] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[4]	"Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum." Iranfar N., Fuller D., Loomis W.F. Eukaryot. Cell 2:664-670(2003) [PubMed: 12912885] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[5]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23957 mRNA. Translation: AAB04942.1. AAFI02000003 Genomic DNA. Translation: EAL73142.1.
RefSeq	XP_647163.1.
3D structure databases
ModBase	Search...
2-D gel databases
SWISS-2DPAGE	P54638.
Genome annotation databases
GeneID	3398210.
GenomeReviews	Gene locus argE in contig CM000150_GR.
KEGG	ddi:DDB_0191165.
Organism-specific databases
dictyBase	DDB_G0267380. argE.
Phylogenomic databases
OMA	IRLTPFY.
Enzyme and pathway databases
BRENDA	3.5.1.16. 424.
Family and domain databases
InterPro	IPR001261. ArgE/DapE_CS. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PROSITE	PS00758. ARGE_DAPE_CPG2_1. False negative. PS00759. ARGE_DAPE_CPG2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGE_DICDI

Accession

Primary (citable) accession number: P54638
Secondary accession number(s): Q55GM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)