P54637 (PTP3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase 3
EC=3.1.3.48

Alternative name(s):
Protein-tyrosine-phosphate phosphohydrolase 3

Gene names

Name:	ptpC
Synonyms:	ptp3
ORF Names:	DDB_G0282145

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	990 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Seems to dephosphorylate a protein of 130 kDa (p130).
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm.
Tissue specificity	In the anterior-like and prestalk cell types.
Developmental stage	Expressed at moderate levels during growth and development.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein kinase cascade Inferred from mutant phenotype PubMed 18305004. Source: dictyBase peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC protein localization to nucleus Inferred from mutant phenotype PubMed 18305004. Source: dictyBase response to osmotic stress Inferred from mutant phenotype PubMed 18305004. Source: dictyBase serine phosphorylation of STAT protein Inferred from mutant phenotype PubMed 18305004. Source: dictyBase
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphatase activity Inferred from direct assay PubMed 18305004. Source: dictyBase protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 990	990	Tyrosine-protein phosphatase 3		PRO_0000094888
Regions
	Domain	422 – 715	294	Tyrosine-protein phosphatase
	Compositional bias	64 – 71	8	Poly-Asn
	Compositional bias	109 – 118	10	Poly-Asn
	Compositional bias	137 – 191	55	Poly-Asn
	Compositional bias	250 – 258	9	Poly-Ser
	Compositional bias	259 – 266	8	Poly-Thr
	Compositional bias	287 – 290	4	Poly-Asn
	Compositional bias	367 – 372	6	Poly-Ser
	Compositional bias	788 – 791	4	Poly-Gln
	Compositional bias	835 – 840	6	Poly-Gln
	Compositional bias	884 – 893	10	Poly-Gln
	Compositional bias	907 – 915	9	Poly-Asn
	Compositional bias	944 – 964	21	Poly-Asn
Sites
	Active site	650	1	Phosphocysteine intermediate By similarity
Experimental info
	Sequence conflict	187	1	Missing in AAC47041. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54637 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 123EAF31B9ED0AF4	FASTA	990	110,110
	10 20 30 40 50 60 MISSSMSYRH STNSVYTLNP HLNIPISTST TIPPTSFYAN NTPEMIQSQS ENTNTNNINN 70 80 90 100 110 120 SSSNINNNNN NTPDSMSMST SLSSSPSVSF NHLDLNSINN KINNNTTTNN NNNNNNNNDD 130 140 150 160 170 180 KFDTNALKLS NTMIIKNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 190 200 210 220 230 240 NNNNNNNSNS NIEINVPSIQ FDNEPAMEVD SVAPLNVPSN HTRTTLAMHN TKSLSTSNIG 250 260 270 280 290 300 LLNILPNQQS SSSSSLSSTT TTTTTTSSSL LMPQSLFNNS TYNNHHNNNN SSNAGIVGGL 310 320 330 340 350 360 NGSTSSLPTQ AQVQLQQMQQ QMQQHQQHQY KKANLSSLST VVDNNLNNNP MNTSTSSPAQ 370 380 390 400 410 420 PNASPFSFSS SSLFSNSSLS NSGSGSASTT STSTSSSNSM SSSPPPSLKT SFSQLDEDRE 430 440 450 460 470 480 KMRLEFEMIK KPEMASKKSH KHHQRHYSHN DLDNRKHDEE KFFSALQPNN YGKNRYHDVL 490 500 510 520 530 540 PNESTRVRLT PIESGDGDYI NANYINGEVP NSYRYYIACQ APLPSTIKDF WRMVWEERSS 550 560 570 580 590 600 VIVCLTKLEE NGKKKADVYY PETSQAQEYG SFWIHLHKKV MFKDIGVSSL HLYKKGEEFP 610 620 630 640 650 660 REVVLLHYTQ WPDCGAPPSS SHIRTLSVMV NTFKARGSAK NTNGPVIVHC SAGIGRSGTF 670 680 690 700 710 720 ISININMAKI ERFGNDPSQM NISIKDSVLE LRRQRRGMVQ TLDQYIFIFK VINDVLTDMG 730 740 750 760 770 780 IRSLSSPSKR RSCEMIKSTP MPRLDISIPP PLTFTPKDFQ SSISPSTDMI ASLSIITQMT 790 800 810 820 830 840 QTLKFPPQQQ QDNPFSKSSI KISPSPLNST NISIPKNQQF QHPFQIQPQL DLNLQQQQQQ 850 860 870 880 890 900 SSQQLNDNPP LNMSSNSIKF PPVTSLSSCH LFEDSKNNDN NNKQQQQQQQ QQQKNNQQCS 910 920 930 940 950 960 GFSHFLNNNN NNDNNGSSGG GFNGSFLFNS NNSGSSSTNS ECSNNNKNNN NNSNNNNNNN 970 980 990 NNKNSDNNGT KDKDENDSCE SPRVTPIKCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple roles of the novel protein tyrosine phosphatase PTP3 during Dictyostelium growth and development." Gamper M., Howard P.K., Hunter T., Firtel R.A. Mol. Cell. Biol. 16:2431-2444(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38197 mRNA. Translation: AAC47041.1. AAFI02000045 Genomic DNA. Translation: EAL66352.1.
RefSeq	XP_640326.1. XM_635234.1.
3D structure databases
ProteinModelPortal	P54637.
ModBase	Search...
Proteomic databases
PRIDE	P54637.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0214986; DDB0214986; DDB_G0282145.
GeneID	8623427.
KEGG	ddi:DDB_G0282145.
Organism-specific databases
dictyBase	DDB_G0282145. ptpC.
Phylogenomic databases
eggNOG	COG5599.
OMA	SININMA.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTP3_DICDI

Accession

Primary (citable) accession number: P54637
Secondary accession number(s): Q54SY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)