Reviewed, UniProtKB/Swiss-Prot P54630 (TRYZ_DROER)

Names and origin

Protein names

Recommended name:
    Trypsin zeta
    EC=3.4.21.4

Gene names

Name:

zetaTry

Organism

Drosophila erecta (Fruit fly)

Taxonomic identifier

7220 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Probable
	Propeptide	24 – 39	16	Activation peptide		PRO_0000028285
	Chain	40 – 281	242	Trypsin zeta		PRO_0000028286
Regions
	Domain	40 – 279	240	Peptidase S1
Sites
	Active site	88	1	Charge relay system By similarity
	Active site	135	1	Charge relay system By similarity
	Active site	235	1	Charge relay system By similarity
	Site	229	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 89		By similarity
	Disulfide bond	199 ↔ 219		By similarity
	Disulfide bond	231 ↔ 255		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54630-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A1E0BC1812AAD468	FASTA	281	29,736
	10 20 30 40 50 60 MSSSSWLGCL LAVLLSALAL SQGLPLLEDL DENSFPDGRI VGGYVTDIAQ VPYQITLRYK 70 80 90 100 110 120 AISSPENPFR HRCGGSIVNE TTILTAAHCV IGTVASQFKV VAGTNFQTGT DGVITNVKRV 130 140 150 160 170 180 IMHEGYNSGA AYNNDIAVLF VDPPLPLNNF TIKAIKLATE PPLDGAPSKI SGWGSTYPGG 190 200 210 220 230 240 YSSNQLLAVD VPIVGNDLCD LDYENFIDET YHITSAMLCA GKRGVGGADA CQGDSGGPLV 250 260 270 280 VRDELHGVVS WGNSCALPNY PGVYANVAFL RPWIDAVRAG L

References

[1]	"Concerted evolution within a trypsin gene cluster in Drosophila." Wang S., Magoulas C., Hickey D.A. Mol. Biol. Evol. 16:1117-1124(1999) [PubMed: 10486967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40653 Genomic DNA. Translation: AAA83236.1.
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.116.
Organism-specific databases
FlyBase	FBgn0015083. Dere\zetaTry.
Enzyme and pathway databases
BRENDA	3.4.21.4. 278534.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYZ_DROER

Accession

Primary (citable) accession number: P54630

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project