Reviewed, UniProtKB/Swiss-Prot P54629 (TRYU_DROER)

Names and origin

Protein names

Recommended name:
    Trypsin eta
    EC=3.4.21.4

Gene names

Name:

etaTry

Organism

Drosophila erecta (Fruit fly)

Taxonomic identifier

7220 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 27	5	Activation peptide		PRO_0000028281
	Chain	28 – 258	231	Trypsin eta		PRO_0000028282
Regions
	Domain	28 – 258	231	Peptidase S1
Sites
	Active site	74	1	Charge relay system By similarity
	Active site	120	1	Charge relay system By similarity
	Active site	215	1	Charge relay system By similarity
	Site	209	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	59 ↔ 75		By similarity
	Disulfide bond	185 ↔ 200		By similarity
	Disulfide bond	211 ↔ 235		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54629-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4D45051AF8F58145	FASTA	258	27,343
	10 20 30 40 50 60 MNKVILRILA LLFLLGIGAV SAQPDGRIVG GADTTNYHTK YVVQLRRRSS PSSSYAQTCG 70 80 90 100 110 120 GCILDAVTIA TAAHCVYNRE AENFLVVAGD DSRGGMSGVV VRVSKLIPHE LYNATIMDND 130 140 150 160 170 180 IALVIVDPPL PLASSSTMEA IEIAAEQPAV GVQATISGWG YTKENGLSSD QLQQVNVPVV 190 200 210 220 230 240 DSEKCQEAYY WRPISEGMLC AGLSEGGKDA CQGDSGGPLV VANKLAGIVS WGEGCARPNY 250 PGVYANVAYF KDWIASRV

References

[1]	"Concerted evolution within a trypsin gene cluster in Drosophila." Wang S., Magoulas C., Hickey D.A. Mol. Biol. Evol. 16:1117-1124(1999) [PubMed: 10486967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U40653 Genomic DNA. Translation: AAA83237.1.
3D structure databases
SMR	P54629. Positions 23-258.
ModBase	Search...
Protein family/group databases
MEROPS	S01.117.
Organism-specific databases
FlyBase	FBgn0015079. Dere\etaTry.
Enzyme and pathway databases
BRENDA	3.4.21.4. 278534.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYU_DROER

Accession

Primary (citable) accession number: P54629

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project