P54628 (TRYT_DROER) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trypsin theta
EC=3.4.21.4

Gene names

Name:

thetaTry

Organism

Drosophila erecta (Fruit fly)

Taxonomic identifier

7220 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Probable
	Propeptide	20 – 34	15	Activation peptide		PRO_0000028277
	Chain	35 – 262	228	Trypsin theta		PRO_0000028278
Regions
	Domain	35 – 260	226	Peptidase S1
Sites
	Active site	76	1	Charge relay system By similarity
	Active site	121	1	Charge relay system By similarity
	Active site	216	1	Charge relay system By similarity
	Site	210	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	61 ↔ 77		By similarity
	Disulfide bond	186 ↔ 203		By similarity
	Disulfide bond	212 ↔ 236		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54628 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: EF3BCABD1143F25D	FASTA	262	28,210
	10 20 30 40 50 60 MHGLVVLLVC LAVGSAFAGT IGVSNADPFE REGRIVGGED TTIRAHPYQV SLQNKKGSHF 70 80 90 100 110 120 CGGSLINEDT VVTAAHCLVG KKIAKVFVRL GSTLYNEGGI VVAVRALTYN ADYSSKTMEN 130 140 150 160 170 180 DVGILKLAEK VKETDDIRYI ELATETPPTG TTAVVTGWGS KCYFWCMTLP KTLQAVYVNI 190 200 210 220 230 240 VDWKTCASDE YKYGEVIYDT MVCAYEKKKD ACQGDSGGPL AIGNTLVGIV SWGYACASNL 250 260 LPGVYSDVPA LRKWILNASQ TL

References

[1]	"Concerted evolution within a trypsin gene cluster in Drosophila." Wang S., Magoulas C., Hickey D.A. Mol. Biol. Evol. 16:1117-1124(1999) [PubMed: 10486967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U40653 Genomic DNA. Translation: AAA83238.1.
3D structure databases
ProteinModelPortal	P54628.
ModBase	Search...
Protein family/group databases
MEROPS	S01.114.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
FlyBase	FBgn0015082. Dere\thetaTry.
Phylogenomic databases
GeneTree	EMGT00050000002494.
OrthoDB	EOG4573PH.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYT_DROER

Accession

Primary (citable) accession number: P54628

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project