Reviewed, UniProtKB/Swiss-Prot P54627 (TRYE_DROER)

Names and origin

Protein names

Recommended name:
    Trypsin epsilon
    EC=3.4.21.4

Gene names

Name:

epsilonTry

Organism

Drosophila erecta (Fruit fly)

Taxonomic identifier

7220 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Probable
	Propeptide	23 – 30	8	Activation peptide		PRO_0000028271
	Chain	31 – 256	226	Trypsin epsilon		PRO_0000028272
Regions
	Domain	31 – 254	224	Peptidase S1
Sites
	Active site	71	1	Charge relay system By similarity
	Active site	116	1	Charge relay system By similarity
	Active site	210	1	Charge relay system By similarity
	Site	204	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	180 ↔ 197		By similarity
	Disulfide bond	206 ↔ 230		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54627-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6A9193173D0E37F5	FASTA	256	27,612
	10 20 30 40 50 60 MLKIAVLLSV LACALAGTIP DGLLPQLDGR IVGGYETSID AHPYQVSLQR FGSHFCGGSI 70 80 90 100 110 120 YSHDIVITAA HCLQSVDAKD LKIRVGSTYW RSGGSVHSVR SFRNHEGYNA RTMVNDIAIV 130 140 150 160 170 180 RIESDLSFRS SIRAVRIADH NPREGATAVV SGWGTTESGG STIPDHLLAV DLEIVDVSRC 190 200 210 220 230 240 RSGEFGYGKK IKDTMLCAYA PNKDACQGDS GGPLVSGDRL VGVVSWGYGC GDVRYPGVYA 250 DVAHFHEWIE RTAREV

References

[1]	"Concerted evolution within a trypsin gene cluster in Drosophila." Wang S., Magoulas C., Hickey D.A. Mol. Biol. Evol. 16:1117-1124(1999) [PubMed: 10486967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U40653 Genomic DNA. Translation: AAA83240.1.
3D structure databases
SMR	P54627. Positions 27-255.
ModBase	Search...
Protein family/group databases
MEROPS	S01.110.
Organism-specific databases
FlyBase	FBgn0015080. Dere\epsilonTry.
Enzyme and pathway databases
BRENDA	3.4.21.4. 278534.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYE_DROER

Accession

Primary (citable) accession number: P54627

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project