P54625 (TRYB_DROER) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trypsin beta
EC=3.4.21.4

Gene names

Name:	betaTry
ORF Names:	GG20194

Organism

Drosophila erecta (Fruit fly) [Complete proteome]

Taxonomic identifier

7220 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Probable
	Propeptide	23 – 30	8	Activation peptide		PRO_0000028263
	Chain	31 – 253	223	Trypsin beta		PRO_0000028264
Regions
	Domain	31 – 253	223	Peptidase S1
Sites
	Active site	71	1	Charge relay system By similarity
	Active site	116	1	Charge relay system By similarity
	Active site	210	1	Charge relay system By similarity
	Site	204	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	180 ↔ 197		By similarity
	Disulfide bond	206 ↔ 230		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P54625 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 073832534B59655E	FASTA	253	25,814
	10 20 30 40 50 60 MLKFVILLSA VACALGGTIP EGLLPQLDGR IVGGTATTIS SFPWQISLQR SGSHSCGGSI 70 80 90 100 110 120 YTDRVIVTAA HCLQSVSASS LQIRAGSSYW SSGGVTVKVS SFKNHEGYNP NTMVNDIAVI 130 140 150 160 170 180 RLSSSLGFSS TIKSISLASS NPANGAAASV SGWGTQSSGS SSIPSQLQYV NVNIVSQSKC 190 200 210 220 230 240 ASSAYGYGSE IRNTMICAAA SGKDACQGDS GGPLVSGGVL VGVVSWGYGC AYSNYPGVYA 250 SVADLRSWVI NNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Concerted evolution within a trypsin gene cluster in Drosophila." Wang S., Magoulas C., Hickey D.A. Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 14021-0224.01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U40653 Genomic DNA. Translation: AAA83241.1. CH954179 Genomic DNA. Translation: EDV56479.1.
RefSeq	XP_001976079.1. XM_001976043.1.
3D structure databases
ProteinModelPortal	P54625.
ModBase	Search...
Protein family/group databases
MEROPS	S01.110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0140248; FBpp0138740; FBgn0015077.
GeneID	6547181.
KEGG	der:Dere_GG20194.
Organism-specific databases
FlyBase	FBgn0015077. Dere\betaTry.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYB_DROER

Accession

Primary (citable) accession number: P54625
Secondary accession number(s): B3NSH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project