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Protein

Single-stranded DNA-binding protein, mitochondrial

Gene

mtSSB

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein binds preferentially and cooperatively to pyrimidine rich ss-DNA. Required for mitochondrial DNA replication.3 Publications

GO - Molecular functioni

  • single-stranded DNA binding Source: UniProtKB

GO - Biological processi

  • mitochondrial DNA replication Source: UniProtKB
  • mitochondrial genome maintenance Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA-binding protein, mitochondrial
Short name:
Dm mtSSB
Short name:
Mt-SSB
Short name:
MtSSB
Alternative name(s):
Protein low power
Gene namesi
Name:mtSSB
Synonyms:lopo
ORF Names:CG4337
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0010438. mtSSB.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial chromosome Source: FlyBase
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222Mitochondrion1 PublicationAdd
BLAST
Chaini23 – 146124Single-stranded DNA-binding protein, mitochondrialPRO_0000033268Add
BLAST

Proteomic databases

PaxDbiP54622.

Expressioni

Tissue specificityi

Uniformly distributed in the early embryo. High levels detected in the anterior and posterior midgut primordia of stage 12 embryos. In larvae, high levels were detected in proliferating tissues including the CNS and digestive tract. In adults, highly expressed in the CNS, digestive tract and ovary.3 Publications

Developmental stagei

Expressed both maternally and zygotically. Levels are high during embryogenesis and in the larvae but decrease in the pupae before increasing again in the adult.3 Publications

Gene expression databases

BgeeiFBgn0010438.
GenevisibleiP54622. DM.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi67018. 17 interactions.
DIPiDIP-22042N.
IntActiP54622. 6 interactions.
MINTiMINT-330179.
STRINGi7227.FBpp0099503.

Structurei

3D structure databases

ProteinModelPortaliP54622.
SMRiP54622. Positions 36-140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 142105SSBAdd
BLAST

Sequence similaritiesi

Contains 1 SSB domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1653. Eukaryota.
COG0629. LUCA.
GeneTreeiENSGT00390000002796.
InParanoidiP54622.
KOiK03111.
OMAiRNPVTIF.
OrthoDBiEOG091G1330.
PhylomeDBiP54622.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00984. SSB. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
[Graphical view]
PANTHERiPTHR10302. PTHR10302. 1 hit.
PfamiPF00436. SSB. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00621. ssb. 1 hit.
PROSITEiPS50935. SSB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHTRRMLNP LLTGLRNLPA RGATTTTAAA PAKVEKTVNT VTILGRVGAD
60 70 80 90 100
PQLRGSQEHP VVTFSVATHT NYKYENGDWA QRTDWHRVVV FKPNLRDTVL
110 120 130 140
EYLKKGQRTM VQGKITYGEI TDQQGNQKTS TSIIADDVLF FRDANN
Length:146
Mass (Da):16,368
Last modified:July 25, 2003 - v2
Checksum:iAD505175C0555D48
GO

Sequence cautioni

The sequence AAA20507 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAF16936 differs from that shown. Reason: Erroneous gene model prediction. Curated

Mass spectrometryi

Molecular mass is 13845±14 Da from positions 23 - 146. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF181084 Genomic DNA. Translation: AAF16936.1. Sequence problems.
AE014297 Genomic DNA. Translation: AAF55287.2.
BT016028 mRNA. Translation: AAV36913.2.
U00669 mRNA. Translation: AAA20507.1. Different initiation.
RefSeqiNP_536744.2. NM_080496.4.
UniGeneiDm.5334.

Genome annotation databases

EnsemblMetazoaiFBtr0083277; FBpp0082729; FBgn0010438.
GeneIDi41968.
KEGGidme:Dmel_CG4337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF181084 Genomic DNA. Translation: AAF16936.1. Sequence problems.
AE014297 Genomic DNA. Translation: AAF55287.2.
BT016028 mRNA. Translation: AAV36913.2.
U00669 mRNA. Translation: AAA20507.1. Different initiation.
RefSeqiNP_536744.2. NM_080496.4.
UniGeneiDm.5334.

3D structure databases

ProteinModelPortaliP54622.
SMRiP54622. Positions 36-140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67018. 17 interactions.
DIPiDIP-22042N.
IntActiP54622. 6 interactions.
MINTiMINT-330179.
STRINGi7227.FBpp0099503.

Proteomic databases

PaxDbiP54622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083277; FBpp0082729; FBgn0010438.
GeneIDi41968.
KEGGidme:Dmel_CG4337.

Organism-specific databases

CTDi41968.
FlyBaseiFBgn0010438. mtSSB.

Phylogenomic databases

eggNOGiKOG1653. Eukaryota.
COG0629. LUCA.
GeneTreeiENSGT00390000002796.
InParanoidiP54622.
KOiK03111.
OMAiRNPVTIF.
OrthoDBiEOG091G1330.
PhylomeDBiP54622.

Miscellaneous databases

GenomeRNAii41968.
PROiP54622.

Gene expression databases

BgeeiFBgn0010438.
GenevisibleiP54622. DM.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00984. SSB. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR000424. Primosome_PriB/ssb.
IPR011344. ssDNA-bd.
[Graphical view]
PANTHERiPTHR10302. PTHR10302. 1 hit.
PfamiPF00436. SSB. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00621. ssb. 1 hit.
PROSITEiPS50935. SSB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSBP_DROME
AccessioniPrimary (citable) accession number: P54622
Secondary accession number(s): Q5U164, Q9V3U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 25, 2003
Last modified: September 7, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.