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P54609

- CD48A_ARATH

UniProt

P54609 - CD48A_ARATH

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Protein

Cell division control protein 48 homolog A

Gene

CDC48A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probably functions in cell division and growth processes. Interacts with certain SNAREs as part of specialized membrane fusion events where vesicles from the same organelle fuse (homotypic fusion) (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi248 – 2558ATPSequence Analysis
Nucleotide bindingi521 – 5288ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: InterPro
  3. identical protein binding Source: IntAct

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: TAIR
  3. pollen germination Source: TAIR
  4. pollen tube growth Source: TAIR
  5. protein transport Source: UniProtKB-KW
  6. response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_235587. Hh ligand biogenesis disease.
REACT_246136. Hedgehog ligand biogenesis.
REACT_257743. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 48 homolog A
Short name:
AtCDC48a
Gene namesi
Name:CDC48A
Synonyms:CDC48
Ordered Locus Names:At3g09840
ORF Names:F8A24.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G09840.

Subcellular locationi

Nucleus. Cytoplasmcytoskeletonphragmoplast. Cell membrane; Peripheral membrane protein
Note: Primarily localized to the nucleus and, during cytokinesis, to the phragmoplast, a site where membrane vesicles are targeted in the deposition of new cell wall materials.

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. cell wall Source: TAIR
  3. cytoplasm Source: TAIR
  4. cytosol Source: TAIR
  5. cytosolic ribosome Source: TAIR
  6. Golgi apparatus Source: TAIR
  7. nuclear envelope Source: TAIR
  8. nucleolus Source: TAIR
  9. nucleus Source: TAIR
  10. phragmoplast Source: TAIR
  11. plasma membrane Source: TAIR
  12. plasmodesma Source: TAIR
  13. spindle Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 809808Cell division control protein 48 homolog APRO_0000084579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei41 – 411Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated on at least one threonine residue and on Ser-41 by SERK1.1 Publication
Ubiquitinated.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP54609.
PRIDEiP54609.

Expressioni

Developmental stagei

Highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells.

Gene expression databases

ExpressionAtlasiP54609. baseline and differential.
GenevestigatoriP54609.

Interactioni

Subunit structurei

Homohexamer. Interacts with SERK1, GRF6, KAPP and SYP31, but not with KNOLLE. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-1563238,EBI-1563238
SERK1Q94AG212EBI-1563238,EBI-1555537

Protein-protein interaction databases

BioGridi5476. 15 interactions.
IntActiP54609. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP54609.
SMRiP54609. Positions 21-767.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000223224.
InParanoidiP54609.
KOiK13525.
OMAiASETVIH.
PhylomeDBiP54609.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
TIGRFAMsiTIGR01243. CDC48. 1 hit.
PROSITEiPS00674. AAA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54609-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTPAESSDS KSKKDFSTAI LERKKSPNRL VVDEAINDDN SVVSLHPATM
60 70 80 90 100
EKLQLFRGDT ILIKGKKRKD TVCIALADET CEEPKIRMNK VVRSNLRVRL
110 120 130 140 150
GDVISVHQCP DVKYGKRVHI LPVDDTVEGV TGNLFDAYLK PYFLEAYRPV
160 170 180 190 200
RKGDLFLVRG GMRSVEFKVI ETDPAEYCVV APDTEIFCEG EPVKREDEER
210 220 230 240 250
LDDVGYDDVG GVRKQMAQIR ELVELPLRHP QLFKSIGVKP PKGILLYGPP
260 270 280 290 300
GSGKTLIARA VANETGAFFF CINGPEIMSK LAGESESNLR KAFEEAEKNA
310 320 330 340 350
PSIIFIDEID SIAPKREKTN GEVERRIVSQ LLTLMDGLKS RAHVIVMGAT
360 370 380 390 400
NRPNSIDPAL RRFGRFDREI DIGVPDEIGR LEVLRIHTKN MKLAEDVDLE
410 420 430 440 450
RISKDTHGYV GADLAALCTE AALQCIREKM DVIDLEDDSI DAEILNSMAV
460 470 480 490 500
TNEHFHTALG NSNPSALRET VVEVPNVSWN DIGGLENVKR ELQETVQYPV
510 520 530 540 550
EHPEKFEKFG MSPSKGVLFY GPPGCGKTLL AKAIANECQA NFISVKGPEL
560 570 580 590 600
LTMWFGESEA NVREIFDKAR QSAPCVLFFD ELDSIATQRG GGSGGDGGGA
610 620 630 640 650
ADRVLNQLLT EMDGMNAKKT VFIIGATNRP DIIDSALLRP GRLDQLIYIP
660 670 680 690 700
LPDEDSRLNI FKAALRKSPI AKDVDIGALA KYTQGFSGAD ITEICQRACK
710 720 730 740 750
YAIRENIEKD IEKEKRRSEN PEAMEEDGVD EVSEIKAAHF EESMKYARRS
760 770 780 790 800
VSDADIRKYQ AFAQTLQQSR GFGSEFRFEN SAGSGATTGV ADPFATSAAA

AGDDDDLYN
Length:809
Mass (Da):89,393
Last modified:October 1, 1996 - v1
Checksum:i93222D637EA193B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37587 mRNA. Translation: AAC49120.1.
AC015985 Genomic DNA. Translation: AAF23260.1.
CP002686 Genomic DNA. Translation: AEE74820.1.
AY065076 mRNA. Translation: AAL38252.1.
AY094434 mRNA. Translation: AAM19807.1.
PIRiS60112.
RefSeqiNP_187595.1. NM_111819.4.
UniGeneiAt.21739.
At.33291.

Genome annotation databases

EnsemblPlantsiAT3G09840.1; AT3G09840.1; AT3G09840.
GeneIDi820142.
KEGGiath:AT3G09840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37587 mRNA. Translation: AAC49120.1 .
AC015985 Genomic DNA. Translation: AAF23260.1 .
CP002686 Genomic DNA. Translation: AEE74820.1 .
AY065076 mRNA. Translation: AAL38252.1 .
AY094434 mRNA. Translation: AAM19807.1 .
PIRi S60112.
RefSeqi NP_187595.1. NM_111819.4.
UniGenei At.21739.
At.33291.

3D structure databases

ProteinModelPortali P54609.
SMRi P54609. Positions 21-767.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 5476. 15 interactions.
IntActi P54609. 3 interactions.

Proteomic databases

PaxDbi P54609.
PRIDEi P54609.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G09840.1 ; AT3G09840.1 ; AT3G09840 .
GeneIDi 820142.
KEGGi ath:AT3G09840.

Organism-specific databases

GeneFarmi 1893. 150.
TAIRi AT3G09840.

Phylogenomic databases

eggNOGi COG0464.
HOGENOMi HOG000223224.
InParanoidi P54609.
KOi K13525.
OMAi ASETVIH.
PhylomeDBi P54609.

Enzyme and pathway databases

Reactomei REACT_235587. Hh ligand biogenesis disease.
REACT_246136. Hedgehog ligand biogenesis.
REACT_257743. HSF1 activation.

Gene expression databases

ExpressionAtlasi P54609. baseline and differential.
Genevestigatori P54609.

Family and domain databases

Gene3Di 3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
TIGRFAMsi TIGR01243. CDC48. 1 hit.
PROSITEi PS00674. AAA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells."
    Feiler H.S., Desprez T., Santoni V., Kronenberger J., Caboche M., Traas J.
    EMBO J. 14:5626-5637(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Characterization of AtCDC48. Evidence for multiple membrane fusion mechanisms at the plane of cell division in plants."
    Rancour D.M., Dickey C.E., Park S., Bednarek S.Y.
    Plant Physiol. 130:1241-1253(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SYP31 AND KNOLLE, SUBUNIT.
  6. "The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP."
    Rienties I.M., Vink J., Borst J.W., Russinova E., de Vries S.C.
    Planta 221:394-405(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SERK1; GRF6 AND KAPP, SUBUNIT, IDENTIFICATION IN THE SERK1 COMPLEX.
  7. "The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane."
    Aker J., Borst J.W., Karlova R., de Vries S.C.
    J. Struct. Biol. 156:62-71(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SERK1.
  8. "In vivo hexamerization and characterization of the Arabidopsis AAA ATPase CDC48A complex using forster resonance energy transfer-fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy."
    Aker J., Hesselink R., Engel R., Karlova R., Borst J.W., Visser A.J.W.G., de Vries S.C.
    Plant Physiol. 145:339-350(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH SERK1, PHOSPHORYLATION AT SER-41.
  9. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCD48A_ARATH
AccessioniPrimary (citable) accession number: P54609
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3