P54609 (CD48A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell division control protein 48 homolog A
Short name=AtCDC48a

Gene names

Name:	CDC48A
Synonyms:	CDC48
Ordered Locus Names:	At3g09840
ORF Names:	F8A24.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably functions in cell division and growth processes. Interacts with certain SNAREs as part of specialized membrane fusion events where vesicles from the same organelle fuse (homotypic fusion) By similarity.
Subunit structure	Homohexamer. Interacts with SERK1, GRF6, KAPP and SYP31, but not with KNOLLE. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1. Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › phragmoplast. Cell membrane; Peripheral membrane protein. Note: Primarily localized to the nucleus and, during cytokinesis, to the phragmoplast, a site where membrane vesicles are targeted in the deposition of new cell wall materials. Ref.5 Ref.7
Developmental stage	Highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells.
Post-translational modification	Phosphorylated on at least one threonine residue and on Ser-41 by SERK1. Ref.8 Ubiquitinated.
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Protein transport Transport
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from mutant phenotype PubMed 18660433. Source: TAIR pollen germination Inferred from mutant phenotype PubMed 18660433. Source: TAIR pollen tube growth Inferred from mutant phenotype PubMed 18660433. Source: TAIR protein transport Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR apoplast Inferred from direct assay PubMed 21798377. Source: TAIR cell wall Inferred from direct assay PubMed 16287169. Source: TAIR cytosolic ribosome Inferred from direct assay PubMed 15821981. Source: TAIR nuclear envelope Inferred from direct assay PubMed 18660433. Source: TAIR nucleolus Inferred from direct assay PubMed 18660433. Source: TAIR phragmoplast Inferred from direct assay PubMed 18660433. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660PubMed 17644812. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR spindle Inferred from direct assay PubMed 18660433. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		5	EBI-1563238,EBI-1563238
SERK1	Q94AG2	12	EBI-1563238,EBI-1555537

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 809	809	Cell division control protein 48 homolog A		PRO_0000084579
Regions
	Nucleotide binding	248 – 255	8	ATP Potential
	Nucleotide binding	521 – 528	8	ATP Potential
Amino acid modifications
	Modified residue	41	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P54609 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 93222D637EA193B0	FASTA	809	89,393
	10 20 30 40 50 60 MSTPAESSDS KSKKDFSTAI LERKKSPNRL VVDEAINDDN SVVSLHPATM EKLQLFRGDT 70 80 90 100 110 120 ILIKGKKRKD TVCIALADET CEEPKIRMNK VVRSNLRVRL GDVISVHQCP DVKYGKRVHI 130 140 150 160 170 180 LPVDDTVEGV TGNLFDAYLK PYFLEAYRPV RKGDLFLVRG GMRSVEFKVI ETDPAEYCVV 190 200 210 220 230 240 APDTEIFCEG EPVKREDEER LDDVGYDDVG GVRKQMAQIR ELVELPLRHP QLFKSIGVKP 250 260 270 280 290 300 PKGILLYGPP GSGKTLIARA VANETGAFFF CINGPEIMSK LAGESESNLR KAFEEAEKNA 310 320 330 340 350 360 PSIIFIDEID SIAPKREKTN GEVERRIVSQ LLTLMDGLKS RAHVIVMGAT NRPNSIDPAL 370 380 390 400 410 420 RRFGRFDREI DIGVPDEIGR LEVLRIHTKN MKLAEDVDLE RISKDTHGYV GADLAALCTE 430 440 450 460 470 480 AALQCIREKM DVIDLEDDSI DAEILNSMAV TNEHFHTALG NSNPSALRET VVEVPNVSWN 490 500 510 520 530 540 DIGGLENVKR ELQETVQYPV EHPEKFEKFG MSPSKGVLFY GPPGCGKTLL AKAIANECQA 550 560 570 580 590 600 NFISVKGPEL LTMWFGESEA NVREIFDKAR QSAPCVLFFD ELDSIATQRG GGSGGDGGGA 610 620 630 640 650 660 ADRVLNQLLT EMDGMNAKKT VFIIGATNRP DIIDSALLRP GRLDQLIYIP LPDEDSRLNI 670 680 690 700 710 720 FKAALRKSPI AKDVDIGALA KYTQGFSGAD ITEICQRACK YAIRENIEKD IEKEKRRSEN 730 740 750 760 770 780 PEAMEEDGVD EVSEIKAAHF EESMKYARRS VSDADIRKYQ AFAQTLQQSR GFGSEFRFEN 790 800 SAGSGATTGV ADPFATSAAA AGDDDDLYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells." Feiler H.S., Desprez T., Santoni V., Kronenberger J., Caboche M., Traas J. EMBO J. 14:5626-5637(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Characterization of AtCDC48. Evidence for multiple membrane fusion mechanisms at the plane of cell division in plants." Rancour D.M., Dickey C.E., Park S., Bednarek S.Y. Plant Physiol. 130:1241-1253(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SYP31 AND KNOLLE, SUBUNIT.
[6]	"The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP." Rienties I.M., Vink J., Borst J.W., Russinova E., de Vries S.C. Planta 221:394-405(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SERK1; GRF6 AND KAPP, SUBUNIT, IDENTIFICATION IN THE SERK1 COMPLEX.
[7]	"The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane." Aker J., Borst J.W., Karlova R., de Vries S.C. J. Struct. Biol. 156:62-71(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SERK1.
[8]	"In vivo hexamerization and characterization of the Arabidopsis AAA ATPase CDC48A complex using forster resonance energy transfer-fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy." Aker J., Hesselink R., Engel R., Karlova R., Borst J.W., Visser A.J.W.G., de Vries S.C. Plant Physiol. 145:339-350(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, INTERACTION WITH SERK1, PHOSPHORYLATION AT SER-41.
[9]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37587 mRNA. Translation: AAC49120.1. AC015985 Genomic DNA. Translation: AAF23260.1. CP002686 Genomic DNA. Translation: AEE74820.1. AY065076 mRNA. Translation: AAL38252.1. AY094434 mRNA. Translation: AAM19807.1.
IPI	IPI00547533.
PIR	S60112.
RefSeq	NP_187595.1. NM_111819.4.
UniGene	At.21739. At.33291.
3D structure databases
ProteinModelPortal	P54609.
SMR	P54609. Positions 21-767.
ModBase	Search...
Protein-protein interaction databases
IntAct	P54609. 3 interactions.
Proteomic databases
PaxDb	P54609.
PRIDE	P54609.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G09840.1; AT3G09840.1; AT3G09840.
GeneID	820142.
KEGG	ath:AT3G09840.
Organism-specific databases
GeneFarm	1893. 150.
TAIR	At3g09840.
Phylogenomic databases
eggNOG	COG0464.
HOGENOM	HOG000223224.
InParanoid	P54609.
KO	K13525.
OMA	QIANECH.
PhylomeDB	P54609.
ProtClustDB	CLSN2684913.
Gene expression databases
ArrayExpress	P54609.
Genevestigator	P54609.
GermOnline	AT3G09840. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR009010. Asp_de-COase-like_dom. IPR005938. ATPase_AAA_CDC48. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR004201. Cdc48_dom2. IPR003338. CDC4_N-term_subdom. [Graphical view]
PANTHER	PTHR23077:SF18. PTHR23077:SF18. 1 hit.
Pfam	PF00004. AAA. 2 hits. PF02933. CDC48_2. 1 hit. PF02359. CDC48_N. 1 hit. [Graphical view]
SMART	SM00382. AAA. 2 hits. SM01072. CDC48_2. 1 hit. SM01073. CDC48_N. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01243. CDC48. 1 hit.
PROSITE	PS00674. AAA. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CD48A_ARATH

Accession

Primary (citable) accession number: P54609

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program