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Protein

Cell division control protein 48 homolog A

Gene

CDC48A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably functions in cell division and growth processes. Interacts with certain SNAREs as part of specialized membrane fusion events where vesicles from the same organelle fuse (homotypic fusion) (By similarity).By similarity

Kineticsi

  1. KM=40.5 µM for ATP (at 22 degrees Celsius).1 Publication
  1. Vmax=3.47 µmol/min/µg enzyme with ATP as substrate (at 22 degrees Celsius).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi248 – 2558ATPSequence Analysis
Nucleotide bindingi521 – 5288ATPSequence Analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • hydrolase activity Source: InterPro
  • identical protein binding Source: IntAct

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: TAIR
  • pollen germination Source: TAIR
  • pollen tube growth Source: TAIR
  • protein transport Source: UniProtKB-KW
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_296809. Translesion Synthesis by POLH.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 48 homolog A
Short name:
AtCDC48a
Gene namesi
Name:CDC48A
Synonyms:CDC48
Ordered Locus Names:At3g09840
ORF Names:F8A24.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G09840.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • nuclear envelope Source: TAIR
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • phragmoplast Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • spindle Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi254 – 2541K → A: Decreases ATPase activity. 1 Publication
Mutagenesisi308 – 3081E → Q: Decreases ATPase activity. 1 Publication
Mutagenesisi527 – 5271K → A: Abolishes ATPase activity. 1 Publication
Mutagenesisi581 – 5811E → Q: Abolishes ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 809808Cell division control protein 48 homolog APRO_0000084579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei41 – 411Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated on at least one threonine residue and on Ser-41 by SERK1.1 Publication
Ubiquitinated.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP54609.
PRIDEiP54609.

Expressioni

Developmental stagei

Highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells.

Gene expression databases

ExpressionAtlasiP54609. baseline and differential.

Interactioni

Subunit structurei

Homohexamer (PubMed:15498773, PubMed:17190830). Interacts with SERK1, GRF6, KAPP and SYP31, but not with KNOLLE. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1 (PubMed:12427991, PubMed:15592873, PubMed:16621602, PubMed:17693538, PubMed:23747397). Interacts with PUX1, PUX2, PUX3, PUX4, PUX5, PUX7 and PUX11 via its N-terminus (PubMed:15498773, Ref. 7, PubMed:17190830).8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-1563238,EBI-1563238
PUX7Q94JZ83EBI-1563238,EBI-10684297
SERK1Q94AG212EBI-1563238,EBI-1555537

Protein-protein interaction databases

BioGridi5476. 15 interactions.
IntActiP54609. 4 interactions.
STRINGi3702.AT3G09840.1.

Structurei

3D structure databases

ProteinModelPortaliP54609.
SMRiP54609. Positions 21-767.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000223224.
InParanoidiP54609.
KOiK13525.
OMAiIHTENTP.
PhylomeDBiP54609.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
TIGRFAMsiTIGR01243. CDC48. 1 hit.
PROSITEiPS00674. AAA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPAESSDS KSKKDFSTAI LERKKSPNRL VVDEAINDDN SVVSLHPATM
60 70 80 90 100
EKLQLFRGDT ILIKGKKRKD TVCIALADET CEEPKIRMNK VVRSNLRVRL
110 120 130 140 150
GDVISVHQCP DVKYGKRVHI LPVDDTVEGV TGNLFDAYLK PYFLEAYRPV
160 170 180 190 200
RKGDLFLVRG GMRSVEFKVI ETDPAEYCVV APDTEIFCEG EPVKREDEER
210 220 230 240 250
LDDVGYDDVG GVRKQMAQIR ELVELPLRHP QLFKSIGVKP PKGILLYGPP
260 270 280 290 300
GSGKTLIARA VANETGAFFF CINGPEIMSK LAGESESNLR KAFEEAEKNA
310 320 330 340 350
PSIIFIDEID SIAPKREKTN GEVERRIVSQ LLTLMDGLKS RAHVIVMGAT
360 370 380 390 400
NRPNSIDPAL RRFGRFDREI DIGVPDEIGR LEVLRIHTKN MKLAEDVDLE
410 420 430 440 450
RISKDTHGYV GADLAALCTE AALQCIREKM DVIDLEDDSI DAEILNSMAV
460 470 480 490 500
TNEHFHTALG NSNPSALRET VVEVPNVSWN DIGGLENVKR ELQETVQYPV
510 520 530 540 550
EHPEKFEKFG MSPSKGVLFY GPPGCGKTLL AKAIANECQA NFISVKGPEL
560 570 580 590 600
LTMWFGESEA NVREIFDKAR QSAPCVLFFD ELDSIATQRG GGSGGDGGGA
610 620 630 640 650
ADRVLNQLLT EMDGMNAKKT VFIIGATNRP DIIDSALLRP GRLDQLIYIP
660 670 680 690 700
LPDEDSRLNI FKAALRKSPI AKDVDIGALA KYTQGFSGAD ITEICQRACK
710 720 730 740 750
YAIRENIEKD IEKEKRRSEN PEAMEEDGVD EVSEIKAAHF EESMKYARRS
760 770 780 790 800
VSDADIRKYQ AFAQTLQQSR GFGSEFRFEN SAGSGATTGV ADPFATSAAA

AGDDDDLYN
Length:809
Mass (Da):89,393
Last modified:October 1, 1996 - v1
Checksum:i93222D637EA193B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37587 mRNA. Translation: AAC49120.1.
AC015985 Genomic DNA. Translation: AAF23260.1.
CP002686 Genomic DNA. Translation: AEE74820.1.
AY065076 mRNA. Translation: AAL38252.1.
AY094434 mRNA. Translation: AAM19807.1.
PIRiS60112.
RefSeqiNP_187595.1. NM_111819.4.
UniGeneiAt.21739.
At.33291.

Genome annotation databases

EnsemblPlantsiAT3G09840.1; AT3G09840.1; AT3G09840.
GeneIDi820142.
KEGGiath:AT3G09840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37587 mRNA. Translation: AAC49120.1.
AC015985 Genomic DNA. Translation: AAF23260.1.
CP002686 Genomic DNA. Translation: AEE74820.1.
AY065076 mRNA. Translation: AAL38252.1.
AY094434 mRNA. Translation: AAM19807.1.
PIRiS60112.
RefSeqiNP_187595.1. NM_111819.4.
UniGeneiAt.21739.
At.33291.

3D structure databases

ProteinModelPortaliP54609.
SMRiP54609. Positions 21-767.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5476. 15 interactions.
IntActiP54609. 4 interactions.
STRINGi3702.AT3G09840.1.

Proteomic databases

PaxDbiP54609.
PRIDEiP54609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G09840.1; AT3G09840.1; AT3G09840.
GeneIDi820142.
KEGGiath:AT3G09840.

Organism-specific databases

GeneFarmi1893. 150.
TAIRiAT3G09840.

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000223224.
InParanoidiP54609.
KOiK13525.
OMAiIHTENTP.
PhylomeDBiP54609.

Enzyme and pathway databases

ReactomeiREACT_296809. Translesion Synthesis by POLH.

Miscellaneous databases

PROiP54609.

Gene expression databases

ExpressionAtlasiP54609. baseline and differential.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
TIGRFAMsiTIGR01243. CDC48. 1 hit.
PROSITEiPS00674. AAA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells."
    Feiler H.S., Desprez T., Santoni V., Kronenberger J., Caboche M., Traas J.
    EMBO J. 14:5626-5637(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Characterization of AtCDC48. Evidence for multiple membrane fusion mechanisms at the plane of cell division in plants."
    Rancour D.M., Dickey C.E., Park S., Bednarek S.Y.
    Plant Physiol. 130:1241-1253(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SYP31 AND KNOLLE, SUBUNIT.
  6. "Plant UBX domain-containing protein 1, PUX1, regulates the oligomeric structure and activity of arabidopsis CDC48."
    Rancour D.M., Park S., Knight S.D., Bednarek S.Y.
    J. Biol. Chem. 279:54264-54274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PUX1, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Columbia.
  7. "The plant UBX-domain containing (PUX) protein family regulates the function of Arabidopsis CDC48, a conserved essential AAA-ATPase."
    Posthuma R., Rancour D., Park S., Bates B., Bednarek S.
    (In) Proceedings of the 16th international conference on Arabidopsis research, abstract#116, Madison (2005)
    Cited for: INTERACTION WITH PUX1; PUX2; PUX3; PUX4 AND PUX11.
  8. "The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP."
    Rienties I.M., Vink J., Borst J.W., Russinova E., de Vries S.C.
    Planta 221:394-405(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SERK1; GRF6 AND KAPP, SUBUNIT, IDENTIFICATION IN THE SERK1 COMPLEX.
  9. "The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane."
    Aker J., Borst J.W., Karlova R., de Vries S.C.
    J. Struct. Biol. 156:62-71(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SERK1.
  10. "Protein domain-domain interactions and requirements for the negative regulation of Arabidopsis CDC48/p97 by the plant ubiquitin regulatory X (UBX) domain-containing protein, PUX1."
    Park S., Rancour D.M., Bednarek S.Y.
    J. Biol. Chem. 282:5217-5224(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PUX1; PUX4 AND PUX5, MUTAGENESIS OF LYS-254; GLU-308; LYS-527 AND GLU-581, SUBUNIT.
  11. "In vivo hexamerization and characterization of the Arabidopsis AAA ATPase CDC48A complex using forster resonance energy transfer-fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy."
    Aker J., Hesselink R., Engel R., Karlova R., Borst J.W., Visser A.J.W.G., de Vries S.C.
    Plant Physiol. 145:339-350(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH SERK1, PHOSPHORYLATION AT SER-41.
  12. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
  13. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  14. "Functional characterization of the plant ubiquitin regulatory X (UBX) domain-containing protein AtPUX7 in Arabidopsis thaliana."
    Gallois J.L., Drouaud J., Lecureuil A., Guyon-Debast A., Bonhomme S., Guerche P.
    Gene 526:299-308(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PUX7.

Entry informationi

Entry nameiCD48A_ARATH
AccessioniPrimary (citable) accession number: P54609
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.