Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P54609 (CD48A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division control protein 48 homolog A

Short name=AtCDC48a
Gene names
Name:CDC48A
Synonyms:CDC48
Ordered Locus Names:At3g09840
ORF Names:F8A24.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probably functions in cell division and growth processes. Interacts with certain SNAREs as part of specialized membrane fusion events where vesicles from the same organelle fuse (homotypic fusion) By similarity.

Subunit structure

Homohexamer. Interacts with SERK1, GRF6, KAPP and SYP31, but not with KNOLLE. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1. Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Nucleus. Cytoplasmcytoskeletonphragmoplast. Cell membrane; Peripheral membrane protein. Note: Primarily localized to the nucleus and, during cytokinesis, to the phragmoplast, a site where membrane vesicles are targeted in the deposition of new cell wall materials. Ref.5 Ref.7

Developmental stage

Highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells.

Post-translational modification

Phosphorylated on at least one threonine residue and on Ser-41 by SERK1. Ref.8

Ubiquitinated.

Sequence similarities

Belongs to the AAA ATPase family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Protein transport
Transport
   Cellular componentCell membrane
Cytoplasm
Cytoskeleton
Membrane
Nucleus
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   PTMAcetylation
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from mutant phenotype PubMed 18660433. Source: TAIR

pollen germination

Inferred from mutant phenotype PubMed 18660433. Source: TAIR

pollen tube growth

Inferred from mutant phenotype PubMed 18660433. Source: TAIR

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

response to cadmium ion

Inferred from expression pattern PubMed 16502469. Source: TAIR

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 22430844. Source: TAIR

apoplast

Inferred from direct assay PubMed 21798377. Source: TAIR

cell wall

Inferred from direct assay PubMed 16287169. Source: TAIR

cytoplasm

Inferred from direct assay Ref.1. Source: TAIR

cytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

cytosolic ribosome

Inferred from direct assay PubMed 15821981. Source: TAIR

nuclear envelope

Inferred from direct assay PubMed 18660433. Source: TAIR

nucleolus

Inferred from direct assay PubMed 18660433. Source: TAIR

nucleus

Inferred from direct assay Ref.1. Source: TAIR

phragmoplast

Inferred from direct assay PubMed 18660433. Source: TAIR

plasma membrane

Inferred from direct assay PubMed 17317660PubMed 17644812. Source: TAIR

plasmodesma

Inferred from direct assay PubMed 21533090. Source: TAIR

spindle

Inferred from direct assay PubMed 18660433. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

identical protein binding

Inferred from physical interaction Ref.6Ref.7Ref.8. Source: IntAct

nucleoside-triphosphatase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself5EBI-1563238,EBI-1563238
SERK1Q94AG212EBI-1563238,EBI-1555537

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.10
Chain2 – 809808Cell division control protein 48 homolog A
PRO_0000084579

Regions

Nucleotide binding248 – 2558ATP Potential
Nucleotide binding521 – 5288ATP Potential

Amino acid modifications

Modified residue21N-acetylserine Ref.10
Modified residue411Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P54609 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 93222D637EA193B0

FASTA80989,393
        10         20         30         40         50         60 
MSTPAESSDS KSKKDFSTAI LERKKSPNRL VVDEAINDDN SVVSLHPATM EKLQLFRGDT 

        70         80         90        100        110        120 
ILIKGKKRKD TVCIALADET CEEPKIRMNK VVRSNLRVRL GDVISVHQCP DVKYGKRVHI 

       130        140        150        160        170        180 
LPVDDTVEGV TGNLFDAYLK PYFLEAYRPV RKGDLFLVRG GMRSVEFKVI ETDPAEYCVV 

       190        200        210        220        230        240 
APDTEIFCEG EPVKREDEER LDDVGYDDVG GVRKQMAQIR ELVELPLRHP QLFKSIGVKP 

       250        260        270        280        290        300 
PKGILLYGPP GSGKTLIARA VANETGAFFF CINGPEIMSK LAGESESNLR KAFEEAEKNA 

       310        320        330        340        350        360 
PSIIFIDEID SIAPKREKTN GEVERRIVSQ LLTLMDGLKS RAHVIVMGAT NRPNSIDPAL 

       370        380        390        400        410        420 
RRFGRFDREI DIGVPDEIGR LEVLRIHTKN MKLAEDVDLE RISKDTHGYV GADLAALCTE 

       430        440        450        460        470        480 
AALQCIREKM DVIDLEDDSI DAEILNSMAV TNEHFHTALG NSNPSALRET VVEVPNVSWN 

       490        500        510        520        530        540 
DIGGLENVKR ELQETVQYPV EHPEKFEKFG MSPSKGVLFY GPPGCGKTLL AKAIANECQA 

       550        560        570        580        590        600 
NFISVKGPEL LTMWFGESEA NVREIFDKAR QSAPCVLFFD ELDSIATQRG GGSGGDGGGA 

       610        620        630        640        650        660 
ADRVLNQLLT EMDGMNAKKT VFIIGATNRP DIIDSALLRP GRLDQLIYIP LPDEDSRLNI 

       670        680        690        700        710        720 
FKAALRKSPI AKDVDIGALA KYTQGFSGAD ITEICQRACK YAIRENIEKD IEKEKRRSEN 

       730        740        750        760        770        780 
PEAMEEDGVD EVSEIKAAHF EESMKYARRS VSDADIRKYQ AFAQTLQQSR GFGSEFRFEN 

       790        800 
SAGSGATTGV ADPFATSAAA AGDDDDLYN 

« Hide

References

« Hide 'large scale' references
[1]"The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells."
Feiler H.S., Desprez T., Santoni V., Kronenberger J., Caboche M., Traas J.
EMBO J. 14:5626-5637(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Characterization of AtCDC48. Evidence for multiple membrane fusion mechanisms at the plane of cell division in plants."
Rancour D.M., Dickey C.E., Park S., Bednarek S.Y.
Plant Physiol. 130:1241-1253(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SYP31 AND KNOLLE, SUBUNIT.
[6]"The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP."
Rienties I.M., Vink J., Borst J.W., Russinova E., de Vries S.C.
Planta 221:394-405(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SERK1; GRF6 AND KAPP, SUBUNIT, IDENTIFICATION IN THE SERK1 COMPLEX.
[7]"The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane."
Aker J., Borst J.W., Karlova R., de Vries S.C.
J. Struct. Biol. 156:62-71(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SERK1.
[8]"In vivo hexamerization and characterization of the Arabidopsis AAA ATPase CDC48A complex using forster resonance energy transfer-fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy."
Aker J., Hesselink R., Engel R., Karlova R., Borst J.W., Visser A.J.W.G., de Vries S.C.
Plant Physiol. 145:339-350(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH SERK1, PHOSPHORYLATION AT SER-41.
[9]"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
[10]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37587 mRNA. Translation: AAC49120.1.
AC015985 Genomic DNA. Translation: AAF23260.1.
CP002686 Genomic DNA. Translation: AEE74820.1.
AY065076 mRNA. Translation: AAL38252.1.
AY094434 mRNA. Translation: AAM19807.1.
PIRS60112.
RefSeqNP_187595.1. NM_111819.4.
UniGeneAt.21739.
At.33291.

3D structure databases

ProteinModelPortalP54609.
SMRP54609. Positions 21-767.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid5476. 15 interactions.
IntActP54609. 3 interactions.

Proteomic databases

PaxDbP54609.
PRIDEP54609.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G09840.1; AT3G09840.1; AT3G09840.
GeneID820142.
KEGGath:AT3G09840.

Organism-specific databases

GeneFarm1893. 150.
TAIRAT3G09840.

Phylogenomic databases

eggNOGCOG0464.
HOGENOMHOG000223224.
InParanoidP54609.
KOK13525.
OMADKFLKYG.
PhylomeDBP54609.
ProtClustDBCLSN2684913.

Gene expression databases

ArrayExpressP54609.
GenevestigatorP54609.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsTIGR01243. CDC48. 1 hit.
PROSITEPS00674. AAA. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCD48A_ARATH
AccessionPrimary (citable) accession number: P54609
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names