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Protein

Cell division control protein 48 homolog A

Gene

CDC48A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably functions in cell division and growth processes. Interacts with certain SNAREs as part of specialized membrane fusion events where vesicles from the same organelle fuse (homotypic fusion) (By similarity).By similarity

Kineticsi

  1. KM=40.5 µM for ATP (at 22 degrees Celsius)1 Publication
  1. Vmax=3.47 µmol/min/µg enzyme with ATP as substrate (at 22 degrees Celsius)1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi248 – 255ATPSequence analysis8
Nucleotide bindingi521 – 528ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • hydrolase activity Source: InterPro
  • identical protein binding Source: IntAct

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: TAIR
  • negative regulation of defense response Source: TAIR
  • pollen germination Source: TAIR
  • pollen tube growth Source: TAIR
  • protein destabilization Source: TAIR
  • protein transport Source: UniProtKB-KW
  • response to cadmium ion Source: TAIR

Keywordsi

Biological processCell cycle, Cell division, Protein transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-110320. Translesion Synthesis by POLH.
R-ATH-3371511. HSF1 activation.
R-ATH-382556. ABC-family proteins mediated transport.
R-ATH-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
R-ATH-5358346. Hedgehog ligand biogenesis.
R-ATH-5689877. Josephin domain DUBs.
R-ATH-5689896. Ovarian tumor domain proteases.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-8876725. Protein methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 48 homolog A
Short name:
AtCDC48a
Gene namesi
Name:CDC48A
Synonyms:CDC48
Ordered Locus Names:At3g09840
ORF Names:F8A24.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G09840.
TAIRilocus:2085064. AT3G09840.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi254K → A: Decreases ATPase activity. 1 Publication1
Mutagenesisi308E → Q: Decreases ATPase activity. 1 Publication1
Mutagenesisi527K → A: Abolishes ATPase activity. 1 Publication1
Mutagenesisi581E → Q: Abolishes ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000845792 – 809Cell division control protein 48 homolog AAdd BLAST808

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei41Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated on at least one threonine residue and on Ser-41 by SERK1.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP54609.
PRIDEiP54609.

PTM databases

iPTMnetiP54609.
SwissPalmiP54609.

Expressioni

Developmental stagei

Highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells.

Gene expression databases

ExpressionAtlasiP54609. baseline and differential.
GenevisibleiP54609. AT.

Interactioni

Subunit structurei

Homohexamer (PubMed:15498773, PubMed:17190830). Interacts with SERK1, GRF6, KAPP and SYP31, but not with KNOLLE. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1 (PubMed:12427991, PubMed:15592873, PubMed:16621602, PubMed:17693538, PubMed:23747397). Interacts with PUX1, PUX2, PUX3, PUX4, PUX5, PUX7 and PUX11 via its N-terminus (PubMed:15498773, Ref. 7, PubMed:17190830).8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi5476. 16 interactors.
IntActiP54609. 4 interactors.
STRINGi3702.AT3G09840.1.

Structurei

3D structure databases

ProteinModelPortaliP54609.
SMRiP54609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0730. Eukaryota.
COG0464. LUCA.
HOGENOMiHOG000223224.
InParanoidiP54609.
KOiK13525.
OMAiKARDANR.
OrthoDBiEOG093602AN.
PhylomeDBiP54609.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR005938. AAA_ATPase_CDC48.
IPR009010. Asp_de-COase-like_dom_sf.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like_sf.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
TIGRFAMsiTIGR01243. CDC48. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPAESSDS KSKKDFSTAI LERKKSPNRL VVDEAINDDN SVVSLHPATM
60 70 80 90 100
EKLQLFRGDT ILIKGKKRKD TVCIALADET CEEPKIRMNK VVRSNLRVRL
110 120 130 140 150
GDVISVHQCP DVKYGKRVHI LPVDDTVEGV TGNLFDAYLK PYFLEAYRPV
160 170 180 190 200
RKGDLFLVRG GMRSVEFKVI ETDPAEYCVV APDTEIFCEG EPVKREDEER
210 220 230 240 250
LDDVGYDDVG GVRKQMAQIR ELVELPLRHP QLFKSIGVKP PKGILLYGPP
260 270 280 290 300
GSGKTLIARA VANETGAFFF CINGPEIMSK LAGESESNLR KAFEEAEKNA
310 320 330 340 350
PSIIFIDEID SIAPKREKTN GEVERRIVSQ LLTLMDGLKS RAHVIVMGAT
360 370 380 390 400
NRPNSIDPAL RRFGRFDREI DIGVPDEIGR LEVLRIHTKN MKLAEDVDLE
410 420 430 440 450
RISKDTHGYV GADLAALCTE AALQCIREKM DVIDLEDDSI DAEILNSMAV
460 470 480 490 500
TNEHFHTALG NSNPSALRET VVEVPNVSWN DIGGLENVKR ELQETVQYPV
510 520 530 540 550
EHPEKFEKFG MSPSKGVLFY GPPGCGKTLL AKAIANECQA NFISVKGPEL
560 570 580 590 600
LTMWFGESEA NVREIFDKAR QSAPCVLFFD ELDSIATQRG GGSGGDGGGA
610 620 630 640 650
ADRVLNQLLT EMDGMNAKKT VFIIGATNRP DIIDSALLRP GRLDQLIYIP
660 670 680 690 700
LPDEDSRLNI FKAALRKSPI AKDVDIGALA KYTQGFSGAD ITEICQRACK
710 720 730 740 750
YAIRENIEKD IEKEKRRSEN PEAMEEDGVD EVSEIKAAHF EESMKYARRS
760 770 780 790 800
VSDADIRKYQ AFAQTLQQSR GFGSEFRFEN SAGSGATTGV ADPFATSAAA

AGDDDDLYN
Length:809
Mass (Da):89,393
Last modified:October 1, 1996 - v1
Checksum:i93222D637EA193B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37587 mRNA. Translation: AAC49120.1.
AC015985 Genomic DNA. Translation: AAF23260.1.
CP002686 Genomic DNA. Translation: AEE74820.1.
AY065076 mRNA. Translation: AAL38252.1.
AY094434 mRNA. Translation: AAM19807.1.
PIRiS60112.
RefSeqiNP_187595.1. NM_111819.5.
UniGeneiAt.21739.
At.33291.

Genome annotation databases

EnsemblPlantsiAT3G09840.1; AT3G09840.1; AT3G09840.
GeneIDi820142.
GrameneiAT3G09840.1; AT3G09840.1; AT3G09840.
KEGGiath:AT3G09840.

Similar proteinsi

Entry informationi

Entry nameiCD48A_ARATH
AccessioniPrimary (citable) accession number: P54609
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families