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P54609

- CD48A_ARATH

UniProt

P54609 - CD48A_ARATH

Protein

Cell division control protein 48 homolog A

Gene

CDC48A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Probably functions in cell division and growth processes. Interacts with certain SNAREs as part of specialized membrane fusion events where vesicles from the same organelle fuse (homotypic fusion) By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi248 – 2558ATPSequence Analysis
    Nucleotide bindingi521 – 5288ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. nucleoside-triphosphatase activity Source: InterPro
    4. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: TAIR
    3. pollen germination Source: TAIR
    4. pollen tube growth Source: TAIR
    5. protein transport Source: UniProtKB-KW
    6. response to cadmium ion Source: TAIR

    Keywords - Biological processi

    Cell cycle, Cell division, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division control protein 48 homolog A
    Short name:
    AtCDC48a
    Gene namesi
    Name:CDC48A
    Synonyms:CDC48
    Ordered Locus Names:At3g09840
    ORF Names:F8A24.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G09840.

    Subcellular locationi

    Nucleus. Cytoplasmcytoskeletonphragmoplast. Cell membrane; Peripheral membrane protein
    Note: Primarily localized to the nucleus and, during cytokinesis, to the phragmoplast, a site where membrane vesicles are targeted in the deposition of new cell wall materials.

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. cell wall Source: TAIR
    3. cytoplasm Source: TAIR
    4. cytosol Source: TAIR
    5. cytosolic ribosome Source: TAIR
    6. Golgi apparatus Source: TAIR
    7. nuclear envelope Source: TAIR
    8. nucleolus Source: TAIR
    9. nucleus Source: TAIR
    10. phragmoplast Source: TAIR
    11. plasma membrane Source: TAIR
    12. plasmodesma Source: TAIR
    13. spindle Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 809808Cell division control protein 48 homolog APRO_0000084579Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei41 – 411Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated on at least one threonine residue and on Ser-41 by SERK1.1 Publication
    Ubiquitinated.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP54609.
    PRIDEiP54609.

    Expressioni

    Developmental stagei

    Highly expressed in the proliferating cells of the vegetative shoot, root, floral inflorescence and flowers, and in rapidly growing cells.

    Gene expression databases

    ArrayExpressiP54609.
    GenevestigatoriP54609.

    Interactioni

    Subunit structurei

    Homohexamer. Interacts with SERK1, GRF6, KAPP and SYP31, but not with KNOLLE. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself5EBI-1563238,EBI-1563238
    SERK1Q94AG212EBI-1563238,EBI-1555537

    Protein-protein interaction databases

    BioGridi5476. 15 interactions.
    IntActiP54609. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP54609.
    SMRiP54609. Positions 21-767.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    HOGENOMiHOG000223224.
    InParanoidiP54609.
    KOiK13525.
    OMAiASETVIH.
    PhylomeDBiP54609.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR005938. AAA_ATPase_CDC48.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    TIGRFAMsiTIGR01243. CDC48. 1 hit.
    PROSITEiPS00674. AAA. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P54609-1 [UniParc]FASTAAdd to Basket

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    MSTPAESSDS KSKKDFSTAI LERKKSPNRL VVDEAINDDN SVVSLHPATM    50
    EKLQLFRGDT ILIKGKKRKD TVCIALADET CEEPKIRMNK VVRSNLRVRL 100
    GDVISVHQCP DVKYGKRVHI LPVDDTVEGV TGNLFDAYLK PYFLEAYRPV 150
    RKGDLFLVRG GMRSVEFKVI ETDPAEYCVV APDTEIFCEG EPVKREDEER 200
    LDDVGYDDVG GVRKQMAQIR ELVELPLRHP QLFKSIGVKP PKGILLYGPP 250
    GSGKTLIARA VANETGAFFF CINGPEIMSK LAGESESNLR KAFEEAEKNA 300
    PSIIFIDEID SIAPKREKTN GEVERRIVSQ LLTLMDGLKS RAHVIVMGAT 350
    NRPNSIDPAL RRFGRFDREI DIGVPDEIGR LEVLRIHTKN MKLAEDVDLE 400
    RISKDTHGYV GADLAALCTE AALQCIREKM DVIDLEDDSI DAEILNSMAV 450
    TNEHFHTALG NSNPSALRET VVEVPNVSWN DIGGLENVKR ELQETVQYPV 500
    EHPEKFEKFG MSPSKGVLFY GPPGCGKTLL AKAIANECQA NFISVKGPEL 550
    LTMWFGESEA NVREIFDKAR QSAPCVLFFD ELDSIATQRG GGSGGDGGGA 600
    ADRVLNQLLT EMDGMNAKKT VFIIGATNRP DIIDSALLRP GRLDQLIYIP 650
    LPDEDSRLNI FKAALRKSPI AKDVDIGALA KYTQGFSGAD ITEICQRACK 700
    YAIRENIEKD IEKEKRRSEN PEAMEEDGVD EVSEIKAAHF EESMKYARRS 750
    VSDADIRKYQ AFAQTLQQSR GFGSEFRFEN SAGSGATTGV ADPFATSAAA 800
    AGDDDDLYN 809
    Length:809
    Mass (Da):89,393
    Last modified:October 1, 1996 - v1
    Checksum:i93222D637EA193B0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37587 mRNA. Translation: AAC49120.1.
    AC015985 Genomic DNA. Translation: AAF23260.1.
    CP002686 Genomic DNA. Translation: AEE74820.1.
    AY065076 mRNA. Translation: AAL38252.1.
    AY094434 mRNA. Translation: AAM19807.1.
    PIRiS60112.
    RefSeqiNP_187595.1. NM_111819.4.
    UniGeneiAt.21739.
    At.33291.

    Genome annotation databases

    EnsemblPlantsiAT3G09840.1; AT3G09840.1; AT3G09840.
    GeneIDi820142.
    KEGGiath:AT3G09840.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37587 mRNA. Translation: AAC49120.1 .
    AC015985 Genomic DNA. Translation: AAF23260.1 .
    CP002686 Genomic DNA. Translation: AEE74820.1 .
    AY065076 mRNA. Translation: AAL38252.1 .
    AY094434 mRNA. Translation: AAM19807.1 .
    PIRi S60112.
    RefSeqi NP_187595.1. NM_111819.4.
    UniGenei At.21739.
    At.33291.

    3D structure databases

    ProteinModelPortali P54609.
    SMRi P54609. Positions 21-767.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5476. 15 interactions.
    IntActi P54609. 3 interactions.

    Proteomic databases

    PaxDbi P54609.
    PRIDEi P54609.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G09840.1 ; AT3G09840.1 ; AT3G09840 .
    GeneIDi 820142.
    KEGGi ath:AT3G09840.

    Organism-specific databases

    GeneFarmi 1893. 150.
    TAIRi AT3G09840.

    Phylogenomic databases

    eggNOGi COG0464.
    HOGENOMi HOG000223224.
    InParanoidi P54609.
    KOi K13525.
    OMAi ASETVIH.
    PhylomeDBi P54609.

    Gene expression databases

    ArrayExpressi P54609.
    Genevestigatori P54609.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR005938. AAA_ATPase_CDC48.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    TIGRFAMsi TIGR01243. CDC48. 1 hit.
    PROSITEi PS00674. AAA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The higher plant Arabidopsis thaliana encodes a functional CDC48 homologue which is highly expressed in dividing and expanding cells."
      Feiler H.S., Desprez T., Santoni V., Kronenberger J., Caboche M., Traas J.
      EMBO J. 14:5626-5637(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Characterization of AtCDC48. Evidence for multiple membrane fusion mechanisms at the plane of cell division in plants."
      Rancour D.M., Dickey C.E., Park S., Bednarek S.Y.
      Plant Physiol. 130:1241-1253(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SYP31 AND KNOLLE, SUBUNIT.
    6. "The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP."
      Rienties I.M., Vink J., Borst J.W., Russinova E., de Vries S.C.
      Planta 221:394-405(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SERK1; GRF6 AND KAPP, SUBUNIT, IDENTIFICATION IN THE SERK1 COMPLEX.
    7. "The Arabidopsis thaliana AAA protein CDC48A interacts in vivo with the somatic embryogenesis receptor-like kinase 1 receptor at the plasma membrane."
      Aker J., Borst J.W., Karlova R., de Vries S.C.
      J. Struct. Biol. 156:62-71(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SERK1.
    8. "In vivo hexamerization and characterization of the Arabidopsis AAA ATPase CDC48A complex using forster resonance energy transfer-fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy."
      Aker J., Hesselink R., Engel R., Karlova R., Borst J.W., Visser A.J.W.G., de Vries S.C.
      Plant Physiol. 145:339-350(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH SERK1, PHOSPHORYLATION AT SER-41.
    9. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
    10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCD48A_ARATH
    AccessioniPrimary (citable) accession number: P54609
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3