Reviewed, UniProtKB/Swiss-Prot P54608 (YHCX_BACSU)

Names and origin

Protein names

Recommended name:
    UPF0012 hydrolase yhcX
    EC=3.5.-.-

Gene names

Name:	yhcX
Ordered Locus Names:	BSU09250

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Sequence similarities

Belongs to the UPF0012 family. Contains 1 CN hydrolase domain. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	N-acetyltransferase activity Inferred from electronic annotation. Source: InterPro hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	UPF0012 hydrolase yhcX		PRO_0000213260
Regions
	Domain	14 – 212	199	N-acetyltransferase
	Domain	229 – 508	280	CN hydrolase
Sites
	Active site	270	1	Proton acceptor Potential
	Active site	345	1	By similarity
	Active site	379	1	Nucleophile By similarity
Experimental info
	Sequence conflict	137	1	N → S in CAA65708. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54608-1 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 6462F5AA4B67AAD4	FASTA	513	60,218
	10 20 30 40 50 60 MSEKLDLTRF EKKMVIRNIE EKDIDKIIDL QKDCFPGMEP WKREHLISHL EHFPEGQFCA 70 80 90 100 110 120 EFEGEIIGSC SSLLINFDEY DDRHTWQDIT DDGYITNHNP DGLNMYGIEV MVHPKYRRMK 130 140 150 160 170 180 IGHRLYEARK DLARRLNLKS IIIGGRIPNY HKYAEEMTAR EYVEQVTRHQ IYDPVLSFQL 190 200 210 220 230 240 MNGFTLMRIN PNYLPDDTAS IKYATLMEWN NVDYLPQQTK RYYKSAFPVR ICVIQYEMKK 250 260 270 280 290 300 IYSFEEFANQ VEYYVDVASD ARSDFAVFPE IFTTQLMSFL EERSPSLAVQ RITEYTEDYI 310 320 330 340 350 360 SLFTDLAVKY NVNIIGGSHF VEEEGKIYNI AYLFRRDGTI EKQYKLHITP NERKWWGISA 370 380 390 400 410 420 GDQVRVFDTD CGKIAIQICY DIEFPELARI AADKGAKIIF TPFCTEDRQG YLRVRYCSQA 430 440 450 460 470 480 RAVENQIYTV ISGTVGNLPQ TENMDIQYAQ SGIFAPSDFE FARDGIVGET NPNIEMVVIG 490 500 510 DVDLEILRRQ RQNGTVRQLK DRRRDIYHIQ YKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 22 kb DNA sequence in the cspB-glpPFKD region at 75 degrees on the Bacillus subtilis chromosome." Noback M.A., Terpstra P., Holsappel S., Venema G., Bron S. Microbiology 142:3021-3026(1996) [PubMed: 8969498] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 137.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X96983 Genomic DNA. Translation: CAA65708.1. AL009126 Genomic DNA. Translation: CAB12753.2.
PIR	D69824.
RefSeq	NP_388806.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	939265.
GenomeReviews	Gene locus BSU09250 in contig AL009126_GR.
KEGG	bsu:BSU09250.
NMPDR	fig|224308.1.peg.925.
Organism-specific databases
SubtiList	BG11602. yhcX. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG303959.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. IPR003010. Ntlse/CNhydtse. IPR001110. UPF0012_CS. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit. G3DSA:3.60.110.10. Ntlse/CNhydtse. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. PF00795. CN_hydrolase. 1 hit. [Graphical view]
PROSITE	PS50263. CN_HYDROLASE. 1 hit. PS51186. GNAT. 1 hit. PS01227. UPF0012. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YHCX_BACSU

Accession

Primary (citable) accession number: P54608

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 16, 2009
Last modified:	December 15, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)