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Protein

L-cystine uptake protein TcyP

Gene

tcyP

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mediates uptake of L-cystine, the oxidized form of L-cysteine. Although it is more specific for L-cystine, it could also transport a much broader range of amino acids and sulfur compounds including S-methylcysteine.1 Publication

Kineticsi

  1. KM=0.6 µM for L-cystine1 Publication
  1. Vmax=1.9 nmol/min/mg enzyme for the wild-type1 Publication
  2. Vmax=0.85 nmol/min/mg enzyme for tcyP deletion mutant1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU09130-MONOMER.

Protein family/group databases

TCDBi2.A.23.1.4. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cystine uptake protein TcyP
Alternative name(s):
Symporter YhcL
Transporter of cystine TcyP
Gene namesi
Name:tcyP
Synonyms:yhcL
Ordered Locus Names:BSU09130
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalSequence analysisAdd
BLAST
Transmembranei34 – 5421HelicalSequence analysisAdd
BLAST
Transmembranei73 – 9321HelicalSequence analysisAdd
BLAST
Transmembranei105 – 12521HelicalSequence analysisAdd
BLAST
Transmembranei184 – 20421HelicalSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Transmembranei338 – 35821HelicalSequence analysisAdd
BLAST
Transmembranei369 – 38921HelicalSequence analysisAdd
BLAST
Transmembranei394 – 41421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463L-cystine uptake protein TcyPPRO_0000202123Add
BLAST

Proteomic databases

PaxDbiP54596.

Expressioni

Inductioni

More strongly expressed in the presence of methionine than in presence of sulfate. Strongly inhibited by seleno-DL-cystine.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005048.

Structurei

3D structure databases

ProteinModelPortaliP54596.
SMRiP54596. Positions 24-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1823. LUCA.
HOGENOMiHOG000208777.
InParanoidiP54596.
KOiK06956.
OMAiLAIMTKT.
OrthoDBiEOG6HQSKK.
PhylomeDBiP54596.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiIPR001991. Na-dicarboxylate_symporter.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.

Sequencei

Sequence statusi: Complete.

P54596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLLVVLHV FILFLLILGL FVMQKKHVSF SKRVFTALGL GIVFGFALQL
60 70 80 90 100
IYGPTSNIVI QTADWFNIAG GGYVKLLQMV VMPLVFISIL GAFTKLKLTK
110 120 130 140 150
NLGKISGLII GILVATTAVA AAVGIASALS FDLQAIQVDQ GSTELSRGQE
160 170 180 190 200
LQQKSEDMTA KTLPQQIVEL LPGNPFLDFT GARPTSTIAV VIFAAFLGVA
210 220 230 240 250
FLGVKHKQPE QAETFKKLVD AVYAIVMRVV TLILRLTPYG VLAIMTKTIA
260 270 280 290 300
TSDLDSILKL GMFVIASYAA LITMFIIHLL LLTFSGLNPV IYLKKAVPVL
310 320 330 340 350
VFAFTSRSSA GALPLNIKTQ RSMGVPEGIA NFAGSFGLSI GQNGCAGIYP
360 370 380 390 400
AMLAMMIAPT VGQNPFDPVF IITVIAVVAI SSFGVAGVGG GATFAALLVL
410 420 430 440 450
SSLNMPVALA GLLISIEPLI DMGRTALNVS GSMTSGLITS KVTKEIDQGA
460
FHDQSRVIEA EEA
Length:463
Mass (Da):48,982
Last modified:October 1, 1996 - v1
Checksum:iFA69EEAF5EC45F89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96983 Genomic DNA. Translation: CAA65696.1.
AL009126 Genomic DNA. Translation: CAB12741.1.
PIRiH69822.
RefSeqiNP_388794.1. NC_000964.3.
WP_003244685.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB12741; CAB12741; BSU09130.
GeneIDi939257.
KEGGibsu:BSU09130.
PATRICi18973524. VBIBacSub10457_0956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96983 Genomic DNA. Translation: CAA65696.1.
AL009126 Genomic DNA. Translation: CAB12741.1.
PIRiH69822.
RefSeqiNP_388794.1. NC_000964.3.
WP_003244685.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP54596.
SMRiP54596. Positions 24-443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100005048.

Protein family/group databases

TCDBi2.A.23.1.4. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Proteomic databases

PaxDbiP54596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12741; CAB12741; BSU09130.
GeneIDi939257.
KEGGibsu:BSU09130.
PATRICi18973524. VBIBacSub10457_0956.

Phylogenomic databases

eggNOGiCOG1823. LUCA.
HOGENOMiHOG000208777.
InParanoidiP54596.
KOiK06956.
OMAiLAIMTKT.
OrthoDBiEOG6HQSKK.
PhylomeDBiP54596.

Enzyme and pathway databases

BioCyciBSUB:BSU09130-MONOMER.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
InterProiIPR001991. Na-dicarboxylate_symporter.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 22 kb DNA sequence in the cspB-glpPFKD region at 75 degrees on the Bacillus subtilis chromosome."
    Noback M.A., Terpstra P., Holsappel S., Venema G., Bron S.
    Microbiology 142:3021-3026(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Global expression profile of Bacillus subtilis grown in the presence of sulfate or methionine."
    Auger S., Danchin A., Martin-Verstraete I.
    J. Bacteriol. 184:5179-5186(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. "Three different systems participate in L-cystine uptake in Bacillus subtilis."
    Burguiere P., Auger S., Hullo M.-F., Danchin A., Martin-Verstraete I.
    J. Bacteriol. 186:4875-4884(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: 168.

Entry informationi

Entry nameiTCYP_BACSU
AccessioniPrimary (citable) accession number: P54596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.