P54576 (MCPC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methyl-accepting chemotaxis protein McpC

Gene names

Name:	mcpC
Synonyms:	prg71
Ordered Locus Names:	BSU13950

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase. McpC is required for taxis to cysteine, proline, threonine, glycine, serine, lysine, valine and arginine and for aspartate, glutamine, histidine and glutamate. Primarily mediates response to positive stimulus of PTS carbohydrates. Greatly influences the duration or magnitude of the response to negative PTS carbohydrate stimulus. Ref.1 Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein.
Induction	Induced by SigD. Expression increases in the late-exponential growth-phase and is maximal during the early-stationary phase. Ref.1
Post-translational modification	Some glutamine residues are deamidated to glutamate by CheD and subsequently methylated. Ref.1
Sequence similarities	Contains 1 cache domain. Contains 1 HAMP domain. Contains 1 methyl-accepting transducer domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transducer
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 655	655	Methyl-accepting chemotaxis protein McpC		PRO_0000110558
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Potential
	Topological domain	30 – 276	247	Extracellular Potential
	Transmembrane	277 – 297	21	Helical; Potential
	Topological domain	298 – 655	358	Cytoplasmic Potential
	Domain	148 – 225	78	Cache
	Domain	298 – 350	53	HAMP
	Domain	369 – 619	251	Methyl-accepting transducer
Experimental info
	Sequence conflict	95 – 96	2	KQ → NE in CAA66052. Ref.1
	Sequence conflict	115 – 120	6	EMFTYP → RNVYIS in CAA66052. Ref.1
	Sequence conflict	125 – 135	11	AEDYDPTSRPW → LRITIQHQDM in CAA66052. Ref.1
	Sequence conflict	190	1	M → L in CAA66052. Ref.1
	Sequence conflict	376	1	T → R in CAA66052. Ref.1
	Sequence conflict	438	1	D → H in CAA66052. Ref.1
	Sequence conflict	457	1	V → E in CAA66052. Ref.1
	Sequence conflict	492	1	A → R in CAA66052. Ref.1
	Sequence conflict	645 – 647	3	ELM → DVI in CAA66052. Ref.1
	Sequence conflict	651	1	A → R in CAA66052. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54576 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: CD5485BD8D8D693A	FASTA	655	72,031
	10 20 30 40 50 60 MFKKLHMKIA VFVSIMLIIT VVLLMLSSYL TLKPMITEDG KNTTQNVTQS LEQNIELQLK 70 80 90 100 110 120 SYAISLSRLA NGELTHTFVT KPSKEASRLF HDDIKQIKDN DDYVAMAYIG TAKKEMFTYP 130 140 150 160 170 180 KADFAEDYDP TSRPWYKLAA ETPDQVVWTE PYKDVVTGDM IVTASKAILD RQKVIGVASY 190 200 210 220 230 240 DLKLSAIQSM VNKQKVPYKG FAFLADASGN LLAHPSNQGK NISKDQTLQT IASEKKGIQD 250 260 270 280 290 300 VNGKMVVYQT IGETGWKVGT QFDTDQLMWI SDKMNRANLW ISLIALIITI ILSYFLAKTI 310 320 330 340 350 360 TGPIQQLIVK TKAVSAGDLT VRAESKSKDE VGILTRDFNL MVENMKEMVE QVRLSSGKVS 370 380 390 400 410 420 DTSEQLTAVA AETNETSGQI AKAIEEVAAG ASEQASEVET INEKSESLST KIRQIAEEAG 430 440 450 460 470 480 GIKERSKSSE DASYKGLDAL GQLLMKSNEA NMETKKVETM LLDLENQTKN IEEVVTAISN 490 500 510 520 530 540 ISDQTNLLAL NASIEAARAG ESGRGFAVVA DEVRKLAEQS ALSTKHISET VKLIQLETKE 550 560 570 580 590 600 ASHAMVEASR MNDEQNSAIH ETGEVLNTIT AEMQSLVQGI DHIYAEIQRM SEEQLAISEA 610 620 630 640 650 IQSISAISQE SAAAAEEVNA STDEQLVTLD KVKHSTETLK HASQELMNTI AKFTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional and genetic characterization of mcpC, which encodes a third methyl-accepting chemotaxis protein in Bacillus subtilis." Mueller J., Schiel S., Ordal G.W., Saxild H.H. Microbiology 143:3231-3240(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, METHYLATION, EXPRESSION, INDUCTION. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"Unique regulation of carbohydrate chemotaxis in Bacillus subtilis by the phosphoenolpyruvate-dependent phosphotransferase system and the methyl-accepting chemotaxis protein McpC." Garrity L.F., Schiel S.L., Merrill R., Reizer J., Saier M.H. Jr., Ordal G.W. J. Bacteriol. 180:4475-4480(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN RESPONSE TO PTS SUBSTRATES.
[5]	"Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis." Kristich C.J., Ordal G.W. J. Biol. Chem. 277:25356-25362(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DEAMIDATION BY CHED.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97385 Genomic DNA. Translation: CAA66052.1. AL009126 Genomic DNA. Translation: CAB13268.2.
PIR	A69656.
RefSeq	NP_389278.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P54576.
SMR	P54576. Positions 298-350, 357-654.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU13950.
Proteomic databases
PaxDb	P54576.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB13268; CAB13268; BSU13950.
GeneID	936206.
KEGG	bsu:BSU13950.
PATRIC	18974573. VBIBacSub10457_1478.
Organism-specific databases
GenoList	BSU13950. [Micado]
Phylogenomic databases
eggNOG	COG0840.
HOGENOM	HOG000261838.
KO	K03406.
ProtClustDB	CLSK887208.
Enzyme and pathway databases
BioCyc	BSUB:BSU13950-MONOMER.
Family and domain databases
InterPro	IPR004010. Cache_domain. IPR003660. HAMP_linker_domain. IPR004089. MCPsignal_dom. [Graphical view]
Pfam	PF02743. Cache_1. 1 hit. PF00672. HAMP. 1 hit. PF00015. MCPsignal. 1 hit. [Graphical view]
SMART	SM00304. HAMP. 1 hit. SM00283. MA. 1 hit. [Graphical view]
PROSITE	PS00538. CHEMOTAXIS_TRANSDUC_1. False negative. PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit. PS50885. HAMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCPC_BACSU

Accession

Primary (citable) accession number: P54576

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program