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P54576 (MCPC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-accepting chemotaxis protein McpC
Gene names
Name:mcpC
Synonyms:prg71
Ordered Locus Names:BSU13950
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. All amino acids serve as attractants in B.subtilis, they appear to cause an increase in the turnover methyl groups, leading to methylation of an unidentified acceptor, while repellents have been shown to cause a decrease in methyl group turnover. The methyl groups are added by a methyltransferase and removed by a methylesterase. McpC is required for taxis to cysteine, proline, threonine, glycine, serine, lysine, valine and arginine and for aspartate, glutamine, histidine and glutamate. Primarily mediates response to positive stimulus of PTS carbohydrates. Greatly influences the duration or magnitude of the response to negative PTS carbohydrate stimulus. Ref.1 Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein.

Induction

Induced by SigD. Expression increases in the late-exponential growth-phase and is maximal during the early-stationary phase. Ref.1

Post-translational modification

Some glutamine residues are deamidated to glutamate by CheD and subsequently methylated. Ref.1

Sequence similarities

Contains 1 cache domain.

Contains 1 HAMP domain.

Contains 1 methyl-accepting transducer domain.

Ontologies

Keywords
   Biological processChemotaxis
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionTransducer
   PTMMethylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchemotaxis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsignal transducer activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 655655Methyl-accepting chemotaxis protein McpC
PRO_0000110558

Regions

Topological domain1 – 88Cytoplasmic Potential
Transmembrane9 – 2921Helical; Potential
Topological domain30 – 276247Extracellular Potential
Transmembrane277 – 29721Helical; Potential
Topological domain298 – 655358Cytoplasmic Potential
Domain148 – 22578Cache
Domain298 – 35053HAMP
Domain369 – 619251Methyl-accepting transducer

Experimental info

Sequence conflict95 – 962KQ → NE in CAA66052. Ref.1
Sequence conflict115 – 1206EMFTYP → RNVYIS in CAA66052. Ref.1
Sequence conflict125 – 13511AEDYDPTSRPW → LRITIQHQDM in CAA66052. Ref.1
Sequence conflict1901M → L in CAA66052. Ref.1
Sequence conflict3761T → R in CAA66052. Ref.1
Sequence conflict4381D → H in CAA66052. Ref.1
Sequence conflict4571V → E in CAA66052. Ref.1
Sequence conflict4921A → R in CAA66052. Ref.1
Sequence conflict645 – 6473ELM → DVI in CAA66052. Ref.1
Sequence conflict6511A → R in CAA66052. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P54576 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: CD5485BD8D8D693A

FASTA65572,031
        10         20         30         40         50         60 
MFKKLHMKIA VFVSIMLIIT VVLLMLSSYL TLKPMITEDG KNTTQNVTQS LEQNIELQLK 

        70         80         90        100        110        120 
SYAISLSRLA NGELTHTFVT KPSKEASRLF HDDIKQIKDN DDYVAMAYIG TAKKEMFTYP 

       130        140        150        160        170        180 
KADFAEDYDP TSRPWYKLAA ETPDQVVWTE PYKDVVTGDM IVTASKAILD RQKVIGVASY 

       190        200        210        220        230        240 
DLKLSAIQSM VNKQKVPYKG FAFLADASGN LLAHPSNQGK NISKDQTLQT IASEKKGIQD 

       250        260        270        280        290        300 
VNGKMVVYQT IGETGWKVGT QFDTDQLMWI SDKMNRANLW ISLIALIITI ILSYFLAKTI 

       310        320        330        340        350        360 
TGPIQQLIVK TKAVSAGDLT VRAESKSKDE VGILTRDFNL MVENMKEMVE QVRLSSGKVS 

       370        380        390        400        410        420 
DTSEQLTAVA AETNETSGQI AKAIEEVAAG ASEQASEVET INEKSESLST KIRQIAEEAG 

       430        440        450        460        470        480 
GIKERSKSSE DASYKGLDAL GQLLMKSNEA NMETKKVETM LLDLENQTKN IEEVVTAISN 

       490        500        510        520        530        540 
ISDQTNLLAL NASIEAARAG ESGRGFAVVA DEVRKLAEQS ALSTKHISET VKLIQLETKE 

       550        560        570        580        590        600 
ASHAMVEASR MNDEQNSAIH ETGEVLNTIT AEMQSLVQGI DHIYAEIQRM SEEQLAISEA 

       610        620        630        640        650 
IQSISAISQE SAAAAEEVNA STDEQLVTLD KVKHSTETLK HASQELMNTI AKFTL 

« Hide

References

« Hide 'large scale' references
[1]"Functional and genetic characterization of mcpC, which encodes a third methyl-accepting chemotaxis protein in Bacillus subtilis."
Mueller J., Schiel S., Ordal G.W., Saxild H.H.
Microbiology 143:3231-3240(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, METHYLATION, EXPRESSION, INDUCTION.
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[4]"Unique regulation of carbohydrate chemotaxis in Bacillus subtilis by the phosphoenolpyruvate-dependent phosphotransferase system and the methyl-accepting chemotaxis protein McpC."
Garrity L.F., Schiel S.L., Merrill R., Reizer J., Saier M.H. Jr., Ordal G.W.
J. Bacteriol. 180:4475-4480(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN RESPONSE TO PTS SUBSTRATES.
[5]"Bacillus subtilis CheD is a chemoreceptor modification enzyme required for chemotaxis."
Kristich C.J., Ordal G.W.
J. Biol. Chem. 277:25356-25362(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEAMIDATION BY CHED.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97385 Genomic DNA. Translation: CAA66052.1.
AL009126 Genomic DNA. Translation: CAB13268.2.
PIRA69656.
RefSeqNP_389278.2. NC_000964.3.

3D structure databases

ProteinModelPortalP54576.
SMRP54576. Positions 298-350, 357-654.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU13950.

Proteomic databases

PaxDbP54576.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB13268; CAB13268; BSU13950.
GeneID936206.
KEGGbsu:BSU13950.
PATRIC18974573. VBIBacSub10457_1478.

Organism-specific databases

GenoListBSU13950. [Micado]

Phylogenomic databases

eggNOGCOG0840.
HOGENOMHOG000261838.
KOK03406.
OrthoDBEOG67HJTT.
PhylomeDBP54576.

Enzyme and pathway databases

BioCycBSUB:BSU13950-MONOMER.

Family and domain databases

InterProIPR004010. Cache_domain.
IPR003660. HAMP_linker_domain.
IPR004089. MCPsignal_dom.
IPR029151. Sensor-like.
[Graphical view]
PfamPF02743. Cache_1. 1 hit.
PF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
[Graphical view]
SMARTSM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
[Graphical view]
SUPFAMSSF103190. SSF103190. 1 hit.
PROSITEPS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMCPC_BACSU
AccessionPrimary (citable) accession number: P54576
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 16, 2009
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList